FastQCFastQC Report
Wed 25 May 2016
SRR1294599_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294599_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2620509
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC388761.4835285816610437No Hit
CCCATGTACTCTGCGTTGATACCAC355411.3562632297771158No Hit
GAGTACATGGGAAGCAGTGGTATCA172240.6572768878107269No Hit
CATGTACTCTGCGTTGATACCACTG124140.4737247611055715No Hit
GTATCAACGCAGAGTACTTTTTTTT121480.46357406137509927No Hit
GTATCAACGCAGAGTACATGGGAAG112360.42877166229919456No Hit
GCGTTGATACCACTGCTTCCCATGT111040.423734472959261No Hit
ACGCAGAGTACATGGGAAGCAGTGG93420.3564956273762082No Hit
GGTATCAACGCAGAGTACATGGGAA78210.29845346839106446No Hit
TATCAACGCAGAGTACATGGGAAGC78100.29803370261273665No Hit
GCTTCCCATGTACTCTGCGTTGATA73800.28162467673265No Hit
GGTATCAACGCAGAGTACTTTTTTT72730.27754150052527965No Hit
ACTCTGCGTTGATACCACTGCTTCC71900.2743741769251699No Hit
TATCAACGCAGAGTACTTTTTTTTT67350.25701113791252006No Hit
GTGGTATCAACGCAGAGTACATGGG65760.25094361438941826No Hit
ATTCCATTCCATTCCATTCCATTCC54480.20789854184816767No Hit
CAGTGGTATCAACGCAGAGTACATG49700.1896578107535597No Hit
ATACCACTGCTTCCCATGTACTCTG39420.15042879074256182No Hit
CATGGGAAGCAGTGGTATCAACGCA39360.15019982759074668No Hit
GAATGGAATGGAATGGAATGGAATG38800.14806283817380517No Hit
GCAGTGGTATCAACGCAGAGTACAT37720.1439415014411322No Hit
GGGAAGCAGTGGTATCAACGCAGAG36290.13848454632287086No Hit
ACATGGGAAGCAGTGGTATCAACGC35940.13714892793728242No Hit
ACGCAGAGTACTTTTTTTTTTTTTT33850.12917337814905425No Hit
GTACTCTGCGTTGATACCACTGCTT33390.127417993985138No Hit
CTGCTTCCCATGTACTCTGCGTTGA31490.12016749417765786No Hit
GTACATGGTAAGCAGTGGTATCAAC30250.1154355890401445No Hit
CCATGTACTCTGCGTTGATACCACT29460.11242090754124485No Hit
GCAGAGTACATGGGAAGCAGTGGTA29420.11226826544003474No Hit
GATACCACTGCTTCCCATGTACTCT28510.10879565763750476No Hit
TACCACTGCTTCCCATGTACTCTGC27950.10665866822056325No Hit
CTTCCCATGTACTCTGCGTTGATAC27890.1064297050687481No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCGT250.00604514818.9934710
CCGCAGA653.3856431E-614.610647
GGAACGA1001.0024451E-813.30559215
CAGACGT500.00150305113.2964444
CCCCGTA1800.012.14399115
GTCAACG550.003076002712.0874463
CCCGTAC1900.012.00550516
GACTGTC1104.9934715E-711.2236287
ACCGCAG855.343496E-511.1728436
GTCTAGA2300.011.1528131
AGACGTG600.00588918711.080375
TGTCCCG3000.011.07910211
AGCGTCG951.3541694E-511.00504617
CGGAAGA951.3547433E-511.00462515
AACCGGC951.3619338E-510.99937119
CGCCCTA3050.010.5950216
CCGCCCT3050.010.59461615
GCGTCGG1002.3834518E-510.45659118
GTCCCGC3150.010.2576912
ACGTGTC3450.010.1850998
Produced by FastQC (version 0.11.5)