##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294599_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2620509 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.69067116350297 31.0 30.0 33.0 25.0 34.0 2 29.824089518486677 31.0 30.0 34.0 25.0 34.0 3 29.810782561708432 31.0 30.0 34.0 24.0 34.0 4 33.33701506081452 35.0 33.0 37.0 26.0 37.0 5 33.79813120275489 35.0 35.0 37.0 28.0 37.0 6 33.840223406979334 37.0 35.0 37.0 28.0 37.0 7 33.7697203100619 37.0 35.0 37.0 28.0 37.0 8 33.58154083805856 37.0 35.0 37.0 27.0 37.0 9 34.61241575587033 38.0 34.0 39.0 25.0 39.0 10 34.818038403989455 38.0 34.0 39.0 27.0 39.0 11 34.99802137676306 39.0 35.0 39.0 27.0 39.0 12 34.80970376365813 38.0 35.0 39.0 27.0 39.0 13 34.61145372902745 38.0 34.0 39.0 25.0 39.0 14 35.501251092822045 39.0 34.0 41.0 25.0 41.0 15 35.47160799676704 39.0 34.0 41.0 25.0 41.0 16 35.59222845638004 39.0 34.0 41.0 26.0 41.0 17 35.624362289921535 39.0 34.0 41.0 26.0 41.0 18 35.538930795505756 39.0 34.0 41.0 25.0 41.0 19 35.809890750232114 39.0 34.0 41.0 26.0 41.0 20 35.96399287314029 39.0 34.0 41.0 27.0 41.0 21 36.02260629518922 39.0 35.0 41.0 27.0 41.0 22 36.06613715121757 39.0 35.0 41.0 27.0 41.0 23 36.08612219992376 39.0 35.0 41.0 27.0 41.0 24 36.12692381518247 39.0 35.0 41.0 27.0 41.0 25 34.97112316729307 38.0 33.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0629764332356402 1101 2 1.0552222770908273 1101 3 1.328889331814029 1101 4 1.1428818728078554 1101 5 0.8566289510281209 1101 6 0.8738523654031454 1101 7 0.9148262600568273 1101 8 0.9519796427516241 1101 9 1.2570839482845244 1101 10 1.2159542280476643 1101 11 1.0290186360674483 1101 12 1.1290828760939249 1101 13 1.1895321125586023 1101 14 1.3311278658933006 1101 15 1.5115268726291902 1101 16 1.3190972551087938 1101 17 1.4478892474734693 1101 18 1.3520906648909303 1101 19 1.2305558408073267 1101 20 1.1258907758115129 1101 21 0.9698938589650936 1101 22 0.9851853165446371 1101 23 1.0256287401460185 1101 24 1.2021260180160809 1101 25 1.0792633892891246 1102 1 0.7670790095547666 1102 2 0.8079361386606898 1102 3 1.037839119419413 1102 4 0.9542114455611639 1102 5 0.8028983787412258 1102 6 0.5016386342290318 1102 7 0.5127227509065619 1102 8 0.5531705070246886 1102 9 0.846386230711687 1102 10 0.7020404371924229 1102 11 0.5854336054996736 1102 12 0.7926079246161848 1102 13 0.7668216864152626 1102 14 0.9818575178871001 1102 15 0.9838582107403582 1102 16 1.0109382425055458 1102 17 0.9482945400364002 1102 18 1.034097116973001 1102 19 0.8165477637935865 1102 20 0.07976077124766334 1102 21 0.35218442332793387 1102 22 0.47654095591011014 1102 23 0.6085993058128167 1102 24 0.6955266138243559 1102 25 0.6763145019159467 1103 1 0.7082435903742308 1103 2 0.769650255301741 1103 3 0.8896553765794444 1103 4 0.6935248408975525 1103 5 0.5194351373803343 1103 6 0.4258276356308528 1103 7 0.4016388766416412 1103 8 0.5400900963483082 1103 9 0.6858334671283473 1103 10 0.6964456110568094 1103 11 0.6322929964410449 1103 12 0.7334373749396192 1103 13 0.6834005435749191 1103 14 0.697355848959127 1103 15 0.9202137158569244 1103 16 0.8008848436911862 1103 17 0.8962365213316446 1103 18 0.8669999647846538 1103 19 0.7195587228579399 1103 20 -0.1133600927916305 1103 21 0.1775616914404594 1103 22 -0.4142807064997882 1103 23 0.23480033344208096 1103 24 0.39299854449503613 1103 25 0.5041130604951292 1104 1 0.3372507395302087 1104 2 0.28880502203377034 1104 3 0.6628562740147537 1104 4 0.47324864077858564 1104 5 0.35698471251725294 1104 6 0.21975061973932242 1104 7 0.2603041868896625 1104 8 0.3793624862844709 1104 9 0.7431356064403687 1104 10 0.5762999434894027 1104 11 0.21859950478410894 1104 12 0.45615414439836854 1104 13 0.44484810012546205 1104 14 0.5896928247266686 1104 15 0.7898124850105219 1104 16 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0.3967539554543009 2207 6 0.3914679758173705 2207 7 0.32755392343401724 2207 8 0.4174848188357032 2207 9 -1.3662061944996609 2207 10 -0.20649678535331617 2207 11 0.07838806245206342 2207 12 0.566333471903242 2207 13 0.7275718593869058 2207 14 0.8465733710213712 2207 15 0.7611246900855733 2207 16 0.8138153177187348 2207 17 0.7931837042112022 2207 18 0.7931645080365826 2207 19 -0.38514737323210824 2207 20 0.23157033452551445 2207 21 -0.36919598866016656 2207 22 0.17388597510073112 2207 23 -0.5130183690909931 2207 24 -1.5983534051816832 2207 25 -0.12905719063394372 2208 1 0.4557781763516324 2208 2 0.11842265462637158 2208 3 0.6451840955948001 2208 4 0.41629046995454644 2208 5 0.3570731863859322 2208 6 0.2355402341616184 2208 7 0.280327231911528 2208 8 0.4731500324283431 2208 9 -0.21439220478104204 2208 10 0.14840693510019776 2208 11 0.2982084165382801 2208 12 0.3682651129315673 2208 13 0.6161299885451683 2208 14 0.7754321942813931 2208 15 0.6704749914246051 2208 16 0.4756557703165285 2208 17 0.4238676195409141 2208 18 0.7661283400009822 2208 19 -1.3151025667901024 2208 20 -0.04507809151402853 2208 21 -0.24272434944266763 2208 22 0.2520093437536204 2208 23 0.39208110776454674 2208 24 0.5512379085573116 2208 25 0.5645598293017571 2209 1 0.19503496235299167 2209 2 -1.4393186582906594 2209 3 -0.212092602643569 2209 4 0.1084451891848488 2209 5 0.11649612635513051 2209 6 0.16887432077147224 2209 7 0.11434871473007746 2209 8 0.30964037903284236 2209 9 -2.058957047922199 2209 10 -0.4307688611209315 2209 11 -0.053110335244866747 2209 12 0.3646371449043855 2209 13 0.45186831409307615 2209 14 0.6113202335371426 2209 15 0.753356751178984 2209 16 0.5813975891842844 2209 17 0.49289756573499943 2209 18 0.6519648896681076 2209 19 0.3272062759543246 2209 20 0.32608918263876063 2209 21 0.4006167959958802 2209 22 0.3519399481544454 2209 23 0.39036960074385263 2209 24 0.44775717470277954 2209 25 0.4215072829289568 2210 1 0.24698624249219137 2210 2 0.1820207593472638 2210 3 0.1587335858588368 2210 4 0.25940458383313825 2210 5 0.15766770197804192 2210 6 0.14976497362921748 2210 7 0.261882060428853 2210 8 0.34483805584296334 2210 9 0.4686633227636179 2210 10 -1.4427775396458102 2210 11 -0.35214564111933555 2210 12 -2.172163939217242 2210 13 -0.6512573049678849 2210 14 -0.30642683250822245 2210 15 -0.6902971879098345 2210 16 -0.041278369232479406 2210 17 0.13067482618285453 2210 18 0.4171418985169737 2210 19 0.37918626159526525 2210 20 0.37750105170130155 2210 21 0.31984077587805615 2210 22 0.2002660704133845 2210 23 0.14631510949997306 2210 24 0.26932976933945696 2210 25 0.19082545471025014 2211 1 -0.3371903499676705 2211 2 -0.011655308269897802 2211 3 -1.3949903531011998 2211 4 -0.7060944522516905 2211 5 -0.4141648656448993 2211 6 -0.3663827164851696 2211 7 -0.4092248821601956 2211 8 -0.035294492492788265 2211 9 0.31159270053392873 2211 10 0.21873958018097994 2211 11 0.055251619032887334 2211 12 -0.19445308888318635 2211 13 -0.10334618781286053 2211 14 -0.534679635150475 2211 15 -0.19821026933644958 2211 16 0.08693612566974451 2211 17 -0.03641691418843607 2211 18 0.03133318301727428 2211 19 0.0072756563951870135 2211 20 0.10342383716284331 2211 21 -0.013608547248175284 2211 22 -0.07244126467445966 2211 23 -0.3325771924219012 2211 24 -0.27614645097538215 2211 25 0.07189592050926308 2212 1 -0.4258403188996489 2212 2 -0.3512354438785472 2212 3 -2.422908327586292 2212 4 -1.3760808627198067 2212 5 -0.6155046499900578 2212 6 -0.36886263924304785 2212 7 -0.36423749347973455 2212 8 -0.3055321408422955 2212 9 -0.9325268016758415 2212 10 -0.23443823427598431 2212 11 -0.26767209333693387 2212 12 0.08275589788570414 2212 13 -0.4851499224175484 2212 14 -0.13867654078680403 2212 15 0.07297259986292914 2212 16 -1.2621678029875625 2212 17 -1.3794569559826115 2212 18 -0.29731103279046067 2212 19 -0.1187680549927066 2212 20 -0.20599799114939543 2212 21 0.047209076818603535 2212 22 -0.2906521435325615 2212 23 0.055030657034059516 2212 24 0.007468570445205103 2212 25 0.10521799307391433 2213 1 -2.7743312455283906 2213 2 -1.2028544975074453 2213 3 -2.212372759790675 2213 4 -0.9653519279957905 2213 5 -0.7765265649086075 2213 6 -0.6009660567443333 2213 7 -0.4234983720597256 2213 8 -0.1919572415824078 2213 9 -0.4061585841673434 2213 10 -0.2677771267207021 2213 11 -0.6033507706891115 2213 12 -0.26323793600805345 2213 13 -0.561441965075467 2213 14 -0.1276402022279015 2213 15 -0.25145338432515274 2213 16 -1.3717288735822635 2213 17 -0.8983406563675445 2213 18 -0.2768948206187076 2213 19 -0.03535659360749577 2213 20 -0.037421694599352406 2213 21 0.1397434907229922 2213 22 0.0020418318931660906 2213 23 0.09951631903408042 2213 24 0.12432484788392628 2213 25 0.19838794863107267 2214 1 -0.9381197659794012 2214 2 -0.7650847797420575 2214 3 -6.321793674273575 2214 4 -2.0287516523267115 2214 5 -1.1231165828007263 2214 6 -0.8160382567807005 2214 7 -0.5658073296391635 2214 8 -0.3757328114164906 2214 9 -0.29734772286109745 2214 10 -0.16967655053623076 2214 11 -0.8270810064236542 2214 12 -0.505357214937689 2214 13 -5.278720142383708 2214 14 -3.7566060683993108 2214 15 -2.2293467645194553 2214 16 -2.829534916925887 2214 17 -3.252073293449058 2214 18 -1.2305318202397686 2214 19 -0.6840270385784777 2214 20 -0.3929369500929525 2214 21 -0.2844273852411945 2214 22 -0.1264864477846359 2214 23 -0.22591165513131273 2214 24 -0.01999992249594129 2214 25 0.08018372586936096 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1016.0 3 129.0 4 131.0 5 290.0 6 45.0 7 49.0 8 53.0 9 188.0 10 590.0 11 1628.0 12 3446.0 13 6873.0 14 11549.0 15 16896.0 16 21543.0 17 24977.0 18 26248.0 19 26237.0 20 25400.0 21 24513.0 22 23506.0 23 23221.0 24 23328.0 25 24372.0 26 25953.0 27 29093.0 28 33505.0 29 40578.0 30 50336.0 31 63280.0 32 82232.0 33 111017.0 34 147220.0 35 202715.0 36 286230.0 37 435444.0 38 826678.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.01155741020803 21.123034180214614 12.910759832393676 20.954648577183672 2 13.794392394904175 22.47367974934767 41.427123813421936 22.304804042326214 3 19.26295560788255 30.816024888879383 27.6450066265725 22.27601287666557 4 11.414729244523725 18.578291301339842 36.94013552664334 33.066843927493096 5 12.072689343047745 40.924083336864946 32.68886198500993 14.314365335077376 6 29.64497938475415 37.477589011006636 18.639680176332238 14.237751427906975 7 24.474672912791636 35.66393271414318 22.74777311872589 17.1136212543393 8 24.877190664619494 37.26026644041365 21.154134736875868 16.708408158090982 9 25.888135686420664 18.260089569656294 21.57457926395249 34.27719547997055 10 15.895906300548809 32.25012181356339 30.683156429999585 21.170815455888214 11 29.83900960474456 29.70349541832989 20.215631364756536 20.241863612169013 12 21.40991030551111 31.5197660077361 29.97315656633154 17.097167120421247 13 26.13146952906842 26.13360785345452 25.69391490156262 22.04100771591444 14 20.686479212711717 25.70703815317785 27.571509524165922 26.03497310994451 15 25.81001157814023 29.12448333694832 23.76527840997614 21.300226674935313 16 20.19697793553866 32.58200477151995 27.028850589108593 20.192166703832797 17 19.965502980971653 31.3803733574871 28.667580324708826 19.986543336832423 18 20.397112741130368 30.02436621472994 30.12468195134553 19.45383909279416 19 20.18721881948961 29.591120910913155 31.31777593543536 18.903884334161877 20 23.17184280505817 27.873860361110303 29.00968072545872 19.944616108372802 21 22.572478640637677 29.705999713617487 28.580783733473343 19.14073791227149 22 20.936564428423047 28.993745035742652 28.621120241950265 21.448570293884035 23 19.310810170936126 30.19720050283417 29.605392789553513 20.886596536676194 24 20.05045725454791 30.184299590521736 28.831211088680575 20.93403206624978 25 21.263350359879265 28.016738357386384 27.15939016044872 23.56052112228563 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3657.0 1 3657.0 2 4072.0 3 4487.0 4 4487.0 5 4487.0 6 6748.5 7 9010.0 8 9010.0 9 9010.0 10 12294.5 11 15579.0 12 15579.0 13 15579.0 14 21960.5 15 28342.0 16 28342.0 17 28342.0 18 41693.0 19 55044.0 20 55044.0 21 55044.0 22 75790.5 23 96537.0 24 96537.0 25 96537.0 26 123731.5 27 150926.0 28 150926.0 29 150926.0 30 179722.0 31 208518.0 32 208518.0 33 208518.0 34 231849.0 35 255180.0 36 255180.0 37 255180.0 38 273382.0 39 291584.0 40 291584.0 41 291584.0 42 297316.0 43 303048.0 44 303048.0 45 303048.0 46 366233.0 47 429418.0 48 429418.0 49 429418.0 50 380905.5 51 332393.0 52 332393.0 53 332393.0 54 264649.0 55 196905.0 56 196905.0 57 196905.0 58 158339.5 59 119774.0 60 119774.0 61 119774.0 62 95540.5 63 71307.0 64 71307.0 65 71307.0 66 51399.5 67 31492.0 68 31492.0 69 31492.0 70 21672.0 71 11852.0 72 11852.0 73 11852.0 74 7900.0 75 3948.0 76 3948.0 77 3948.0 78 2538.0 79 1128.0 80 1128.0 81 1128.0 82 704.5 83 281.0 84 281.0 85 281.0 86 170.0 87 59.0 88 59.0 89 59.0 90 36.5 91 14.0 92 14.0 93 14.0 94 11.5 95 9.0 96 9.0 97 9.0 98 13.0 99 17.0 100 17.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1033387025192434 2 0.05430242750549607 3 0.057546072156210874 4 0.05315761174642026 5 0.049913967095705454 6 0.06929951394938921 7 0.0653308193179264 8 0.06151476678767369 9 0.06735332715896034 10 0.06689540085533001 11 0.06086603785753073 12 0.06178189046479138 13 0.06243061939493434 14 0.0647584114383885 15 0.06796389556380078 16 0.062468779920236875 17 0.06609402982397694 18 0.1439033409158297 19 0.07105489811330547 20 0.0647584114383885 21 0.06235429834432929 22 0.06826917976622099 23 0.06689540085533001 24 0.0784198794966932 25 0.07071145338558273 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2620509.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.35807765702437 #Duplication Level Percentage of deduplicated Percentage of total 1 88.7924241647784 59.8088700224659 2 8.113019508188806 10.929547961310705 3 1.6297743066080026 3.293353929237746 4 0.524089761703279 1.4120671547224342 5 0.24983304975230275 0.8414136983253421 6 0.13747164073199994 0.5555895271238763 7 0.09095039393406645 0.42883705882834633 8 0.0626210013838458 0.3374424219338973 9 0.051033977536413686 0.3093795559840131 >10 0.28146172802216196 3.672149845925442 >50 0.036181292127142314 1.6805501414408002 >100 0.02426488773582955 3.3950885661956756 >500 0.0032652865613610276 1.5149085782158784 >1k 0.0026924292698920923 3.9445888748136038 >5k 5.155715623197624E-4 2.5303140605309955 >10k+ 4.010001040264818E-4 5.345898602945417 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 38876 1.4835285816610437 No Hit CCCATGTACTCTGCGTTGATACCAC 35541 1.3562632297771158 No Hit GAGTACATGGGAAGCAGTGGTATCA 17224 0.6572768878107269 No Hit CATGTACTCTGCGTTGATACCACTG 12414 0.4737247611055715 No Hit GTATCAACGCAGAGTACTTTTTTTT 12148 0.46357406137509927 No Hit GTATCAACGCAGAGTACATGGGAAG 11236 0.42877166229919456 No Hit GCGTTGATACCACTGCTTCCCATGT 11104 0.423734472959261 No Hit ACGCAGAGTACATGGGAAGCAGTGG 9342 0.3564956273762082 No Hit GGTATCAACGCAGAGTACATGGGAA 7821 0.29845346839106446 No Hit TATCAACGCAGAGTACATGGGAAGC 7810 0.29803370261273665 No Hit GCTTCCCATGTACTCTGCGTTGATA 7380 0.28162467673265 No Hit GGTATCAACGCAGAGTACTTTTTTT 7273 0.27754150052527965 No Hit ACTCTGCGTTGATACCACTGCTTCC 7190 0.2743741769251699 No Hit TATCAACGCAGAGTACTTTTTTTTT 6735 0.25701113791252006 No Hit GTGGTATCAACGCAGAGTACATGGG 6576 0.25094361438941826 No Hit ATTCCATTCCATTCCATTCCATTCC 5448 0.20789854184816767 No Hit CAGTGGTATCAACGCAGAGTACATG 4970 0.1896578107535597 No Hit ATACCACTGCTTCCCATGTACTCTG 3942 0.15042879074256182 No Hit CATGGGAAGCAGTGGTATCAACGCA 3936 0.15019982759074668 No Hit GAATGGAATGGAATGGAATGGAATG 3880 0.14806283817380517 No Hit GCAGTGGTATCAACGCAGAGTACAT 3772 0.1439415014411322 No Hit GGGAAGCAGTGGTATCAACGCAGAG 3629 0.13848454632287086 No Hit ACATGGGAAGCAGTGGTATCAACGC 3594 0.13714892793728242 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3385 0.12917337814905425 No Hit GTACTCTGCGTTGATACCACTGCTT 3339 0.127417993985138 No Hit CTGCTTCCCATGTACTCTGCGTTGA 3149 0.12016749417765786 No Hit GTACATGGTAAGCAGTGGTATCAAC 3025 0.1154355890401445 No Hit CCATGTACTCTGCGTTGATACCACT 2946 0.11242090754124485 No Hit GCAGAGTACATGGGAAGCAGTGGTA 2942 0.11226826544003474 No Hit GATACCACTGCTTCCCATGTACTCT 2851 0.10879565763750476 No Hit TACCACTGCTTCCCATGTACTCTGC 2795 0.10665866822056325 No Hit CTTCCCATGTACTCTGCGTTGATAC 2789 0.1064297050687481 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 3.8160525302527104E-5 0.0 0.0 0.0 7 0.0 3.8160525302527104E-5 0.0 0.0 0.0 8 0.0 3.8160525302527104E-5 0.0 0.0 0.0 9 0.0 3.8160525302527104E-5 0.0 1.1448157590758131E-4 0.0 10 0.0 3.8160525302527104E-5 0.0 2.2896315181516262E-4 0.0 11 0.0 3.8160525302527104E-5 0.0 2.2896315181516262E-4 0.0 12 0.0 3.8160525302527104E-5 0.0 2.6712367711768974E-4 0.0 13 0.0 3.8160525302527104E-5 0.0 2.6712367711768974E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTCGT 25 0.006045148 18.99347 10 CCGCAGA 65 3.3856431E-6 14.61064 7 GGAACGA 100 1.0024451E-8 13.305592 15 CAGACGT 50 0.001503051 13.296444 4 CCCCGTA 180 0.0 12.143991 15 GTCAACG 55 0.0030760027 12.087446 3 CCCGTAC 190 0.0 12.005505 16 GACTGTC 110 4.9934715E-7 11.223628 7 ACCGCAG 85 5.343496E-5 11.172843 6 GTCTAGA 230 0.0 11.152813 1 AGACGTG 60 0.005889187 11.08037 5 TGTCCCG 300 0.0 11.079102 11 AGCGTCG 95 1.3541694E-5 11.005046 17 CGGAAGA 95 1.3547433E-5 11.004625 15 AACCGGC 95 1.3619338E-5 10.999371 19 CGCCCTA 305 0.0 10.59502 16 CCGCCCT 305 0.0 10.594616 15 GCGTCGG 100 2.3834518E-5 10.456591 18 GTCCCGC 315 0.0 10.25769 12 ACGTGTC 345 0.0 10.185099 8 >>END_MODULE