FastQCFastQC Report
Wed 25 May 2016
SRR1294599_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294599_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2620509
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC396271.5121871361632415No Hit
GTACATGGGAAGCAGTGGTATCAAC387481.4786440344223202No Hit
GTATCAACGCAGAGTACTTTTTTTT328011.2517033904481916No Hit
GGTATCAACGCAGAGTACTTTTTTT213510.8147653757342561No Hit
TATCAACGCAGAGTACTTTTTTTTT199470.7611879982095082No Hit
GAGTACATGGGAAGCAGTGGTATCA184060.7023826287183139No Hit
CATGTACTCTGCGTTGATACCACTG140270.5352776884185476No Hit
ACGCAGAGTACTTTTTTTTTTTTTT119320.4553313879097534No Hit
GCGTTGATACCACTGCTTCCCATGT117500.44838617230469346No Hit
ACGCAGAGTACATGGGAAGCAGTGG112970.43109945434264874No Hit
GTATCAACGCAGAGTACATGGGAAG112640.4298401570076653No Hit
TATCAACGCAGAGTACATGGGAAGC87480.3338282753465071No Hit
GGTATCAACGCAGAGTACATGGGAA83250.31768637314353815No Hit
ACTCTGCGTTGATACCACTGCTTCC80840.3084896865456291No Hit
GCTTCCCATGTACTCTGCGTTGATA79090.30181159461768686No Hit
GTGGTATCAACGCAGAGTACATGGG70610.2694514691611439No Hit
CAGTGGTATCAACGCAGAGTACATG60550.2310619807068016No Hit
ATTCCATTCCATTCCATTCCATTCC54210.20686820766499942No Hit
GAGTACTTTTTTTTTTTTTTTTTTT52260.19942690523100665No Hit
GTACTTTTTTTTTTTTTTTTTTTTT51210.1954200500742413No Hit
CATGGGAAGCAGTGGTATCAACGCA49640.18942884760174455No Hit
ACATGGGAAGCAGTGGTATCAACGC45040.17187500596258207No Hit
GCAGTGGTATCAACGCAGAGTACAT44980.1716460428107669No Hit
GAATGGAATGGAATGGAATGGAATG43400.16561667981296765No Hit
GGGAAGCAGTGGTATCAACGCAGAG42330.16153350360559723No Hit
ATACCACTGCTTCCCATGTACTCTG40310.15382507749448676No Hit
ATCAACGCAGAGTACTTTTTTTTTT34840.13295127015400443No Hit
GCAGAGTACATGGGAAGCAGTGGTA34120.1302037123322225No Hit
CTGCTTCCCATGTACTCTGCGTTGA34090.1300892307563149No Hit
GTACTCTGCGTTGATACCACTGCTT33740.12875361237072644No Hit
CCATGTACTCTGCGTTGATACCACT32640.12455595458744848No Hit
GTGGTATCAACGCAGAGTACTTTTT31800.12135047046203619No Hit
CTTCCCATGTACTCTGCGTTGATAC31210.11909899946918709No Hit
GATACCACTGCTTCCCATGTACTCT31080.11860291264025424No Hit
AAGCAGTGGTATCAACGCAGAGTAC30780.11745809688117842No Hit
TACCACTGCTTCCCATGTACTCTGC30290.1155882311413546No Hit
ATGGGAAGCAGTGGTATCAACGCAG30050.11467237853409395No Hit
GTACATGGTAAGCAGTGGTATCAAC28200.10761268135312645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGTT307.613125E-419.041136
TATCGCG1750.013.0567757
CGCGCCT1850.012.81383312
TCGCGCG1800.012.6936019
GCGCCTT1900.012.47662613
TGTATCG2100.012.2407265
GCGCGCC1950.012.16623211
ATCGCGC1950.011.717628
CGCGCGC2150.011.512810
CGATAAG752.111228E-411.37868312
CCCCGTA1950.011.18417615
CCCGTAC1950.011.18417616
GTATCGC2250.011.0015436
CGCCTTT2250.010.95725114
AGCGTCG803.838337E-410.66751617
CCGTACA2151.8189894E-1210.14378717
CACGGTA1502.4454494E-810.133722
GCTACAC1502.4454494E-810.133723
CGTCGGT750.00269004610.11438519
CGCAGCG1803.783498E-1010.0494858
Produced by FastQC (version 0.11.5)