##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294599_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2620509 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.17929837294968 31.0 31.0 34.0 28.0 34.0 2 31.35771943542266 31.0 31.0 34.0 28.0 34.0 3 31.443831713609836 31.0 31.0 34.0 28.0 34.0 4 35.003637461271836 37.0 35.0 37.0 32.0 37.0 5 35.26231010845603 37.0 35.0 37.0 32.0 37.0 6 34.821350355980464 37.0 35.0 37.0 32.0 37.0 7 34.673700033085176 37.0 35.0 37.0 31.0 37.0 8 34.698033092044334 37.0 35.0 37.0 32.0 37.0 9 36.10931387757111 39.0 35.0 39.0 30.0 39.0 10 35.8992085125447 39.0 35.0 39.0 30.0 39.0 11 36.0751418903732 39.0 35.0 39.0 30.0 39.0 12 36.054374550898316 39.0 35.0 39.0 30.0 39.0 13 35.878919706057104 39.0 35.0 39.0 30.0 39.0 14 36.932327269244254 40.0 36.0 41.0 30.0 41.0 15 36.938252835613234 40.0 36.0 41.0 30.0 41.0 16 36.95225889321502 39.0 36.0 41.0 30.0 41.0 17 37.073069010638775 40.0 36.0 41.0 31.0 41.0 18 37.115518015774796 40.0 36.0 41.0 31.0 41.0 19 37.131266101356644 40.0 37.0 41.0 30.0 41.0 20 37.23218046570342 40.0 37.0 41.0 31.0 41.0 21 37.089220452973066 40.0 37.0 41.0 30.0 41.0 22 37.1120053394207 40.0 37.0 41.0 30.0 41.0 23 37.13939467485134 40.0 37.0 41.0 30.0 41.0 24 37.07094194295841 40.0 36.0 41.0 30.0 41.0 25 36.068845785303544 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5586472148253065 1101 2 0.5799168839503999 1101 3 0.49114627159237756 1101 4 0.4227600172352268 1101 5 0.4404589673424866 1101 6 0.5489928966264088 1101 7 0.6127958729626997 1101 8 0.5665101545564184 1101 9 0.8143698015376373 1101 10 0.8440085735360725 1101 11 0.7456811727693164 1101 12 0.8348803451583464 1101 13 0.8255789017978401 1101 14 1.0700677596361814 1101 15 1.031058248739555 1101 16 1.1191422691080604 1101 17 1.036399286535861 1101 18 1.034099209110103 1101 19 1.175689253749809 1101 20 1.0781910493043938 1101 21 1.3606609082663752 1101 22 1.2402689972056322 1101 23 1.2287675319957998 1101 24 1.339695714208034 1101 25 1.3156973237279317 1102 1 0.3569926933526375 1102 2 0.3430150744585916 1102 3 0.2684259403871678 1102 4 0.22511673236564178 1102 5 0.2710516912595722 1102 6 0.31370504800585053 1102 7 0.4314749389279058 1102 8 0.424131786208477 1102 9 0.5658110507289322 1102 10 0.5586797881724976 1102 11 0.4440964882518088 1102 12 0.6212284742300795 1102 13 0.613619354572009 1102 14 0.8118500062546659 1102 15 0.7222547802125732 1102 16 0.8102410037677501 1102 17 0.5799006882901878 1102 18 0.6097278043967975 1102 19 0.7546385523711265 1102 20 0.7187031284646466 1102 21 1.0021926445861098 1102 22 0.9198938461220934 1102 23 0.9192340353334316 1102 24 0.9980926990570467 1102 25 0.9355303920904134 1103 1 0.28838789962600586 1103 2 0.3984662307917297 1103 3 0.23731416412360318 1103 4 0.22118895760085877 1103 5 0.2690353566582786 1103 6 0.2686132632224911 1103 7 0.3609752426252584 1103 8 0.3880867264041825 1103 9 0.398637153187849 1103 10 0.5547459144392803 1103 11 0.5248403403352455 1103 12 0.5450674104824103 1103 13 0.6944186447146379 1103 14 0.8450752705589366 1103 15 0.8138226592290607 1103 16 0.7974675745023347 1103 17 0.8619695334458228 1103 18 0.750495553489614 1103 19 0.8020314075703538 1103 20 0.6688536009626773 1103 21 0.9706566381531019 1103 22 0.9004991656110803 1103 23 0.8339604090244208 1103 24 0.9953286934797774 1103 25 0.962176237817161 1104 1 0.18253591700676353 1104 2 0.19038677991855835 1104 3 0.10002536000042284 1104 4 0.13261186357958366 1104 5 0.19228394950921768 1104 6 0.1572611425969086 1104 7 0.2725422916120408 1104 8 0.19804279690109183 1104 9 0.054891604965831675 1104 10 0.38195256214541473 1104 11 0.44337279825468556 1104 12 0.44657672031306106 1104 13 0.06467090007628684 1104 14 0.32334533958093203 1104 15 0.4843097822118665 1104 16 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2207 4 0.11521550483260512 2207 5 0.056143351346769066 2207 6 0.20704748542345186 2207 7 0.21813986694669296 2207 8 0.22931658899698704 2207 9 0.29876232809923664 2207 10 0.46113814398321296 2207 11 0.30789530882930194 2207 12 0.3325452707594536 2207 13 0.3580550859294718 2207 14 0.4112861514341475 2207 15 0.45511064034906923 2207 16 0.4860790380000637 2207 17 0.490023310599895 2207 18 0.3747299687752417 2207 19 0.5160092053048544 2207 20 0.39164024061815184 2207 21 0.8063943223813084 2207 22 0.6408976503074726 2207 23 0.5158865576232543 2207 24 0.7564737856643404 2207 25 0.7358795804633829 2208 1 0.21577839589344094 2208 2 0.2348640829415949 2208 3 0.1769519215907991 2208 4 0.25737149948247406 2208 5 0.1257821919714175 2208 6 0.29986282528039254 2208 7 0.36058450171715606 2208 8 0.29772251195948485 2208 9 0.3711602657718771 2208 10 0.39010694038962157 2208 11 0.038215630039118764 2208 12 -0.1257790031814494 2208 13 0.04356203272411108 2208 14 -0.3117087187964884 2208 15 0.26862002735013846 2208 16 0.3447578729275307 2208 17 0.2593284294664784 2208 18 0.4014530232011282 2208 19 0.5659863808877503 2208 20 0.532708477957641 2208 21 0.7917301858322645 2208 22 0.6917137518886278 2208 23 0.6484835015251846 2208 24 0.790028829902532 2208 25 0.7618845007900958 2209 1 0.07461249333164233 2209 2 0.018653321193855987 2209 3 0.08789863010197507 2209 4 -0.5747969231031433 2209 5 -0.13190460255766112 2209 6 -0.016184067438281602 2209 7 0.10339863607218547 2209 8 0.17681723679541506 2209 9 0.16238622644268474 2209 10 0.2634020637716077 2209 11 0.24784338275475903 2209 12 0.236918618128243 2209 13 0.257023335183149 2209 14 0.3711521375345299 2209 15 -0.3147684677948348 2209 16 -0.19508245128267987 2209 17 0.13154134558505604 2209 18 -0.2924009623064592 2209 19 0.25493653626672597 2209 20 0.25252539410006847 2209 21 0.47086326499754705 2209 22 0.4600162526725029 2209 23 0.4805828737633675 2209 24 0.6211480010723847 2209 25 0.5427638946284858 2210 1 -0.11453909821493369 2210 2 -0.17819229115443136 2210 3 -0.09071267973953212 2210 4 -0.03259194941757215 2210 5 -0.08844478836742553 2210 6 -0.4305659654334377 2210 7 -0.19900057607220134 2210 8 -0.32763369319975055 2210 9 -0.05082909257954071 2210 10 0.1911455842143397 2210 11 0.1682032803486706 2210 12 0.0556985775241543 2210 13 0.19403747090780854 2210 14 0.1442572274300673 2210 15 0.13816397270275615 2210 16 0.1606780804820076 2210 17 -0.8360085041999952 2210 18 -1.4673209338911661 2210 19 -0.3496880777862259 2210 20 -0.1377072908043715 2210 21 -0.3709299897773448 2210 22 -0.1913538514622246 2210 23 -0.02569132875994029 2210 24 0.3965996868739481 2210 25 0.26429623847371175 2211 1 -0.2817261635309656 2211 2 -0.3175460280255855 2211 3 -0.18303566642781277 2211 4 -0.186263093023598 2211 5 -0.16847263480583052 2211 6 -0.1557585929266665 2211 7 -0.1888594684896674 2211 8 -0.16740068259357344 2211 9 -0.21100455269460383 2211 10 -0.10541720714891056 2211 11 -0.3609103798714486 2211 12 -0.21373549005567583 2211 13 -0.09046260419669494 2211 14 -0.27060248713727475 2211 15 -0.39681472694945086 2211 16 -0.3709326933254502 2211 17 -0.41515376163956574 2211 18 -0.45779999395610815 2211 19 -0.400081198747138 2211 20 -0.3764009524288099 2211 21 -0.03753288427818546 2211 22 -0.19366964767215933 2211 23 -0.7508804010104697 2211 24 -0.18225115203000541 2211 25 -0.709036552685113 2212 1 -0.3011004931465955 2212 2 -0.43562173647168123 2212 3 -0.2726042900216683 2212 4 -0.2038345875780294 2212 5 -0.19933035363440155 2212 6 -0.2960961902773249 2212 7 -0.3049529686207677 2212 8 -0.3474443104524312 2212 9 -0.2809536139855595 2212 10 -0.5681249104620036 2212 11 -0.31562796234669577 2212 12 -0.5364640542104695 2212 13 -0.3615696876814951 2212 14 -0.3982910780345321 2212 15 -0.3612572229321884 2212 16 -0.503311760948634 2212 17 -0.701038894714749 2212 18 -0.506425055748231 2212 19 -0.5929663864374675 2212 20 -0.6782911634763735 2212 21 -0.2657046008682755 2212 22 -0.5837563078130188 2212 23 -0.5159485986803745 2212 24 -0.7554287259497059 2212 25 -0.3288538086145394 2213 1 -0.21303290036202327 2213 2 -0.2638880149020295 2213 3 -0.19263421907538714 2213 4 -0.06736666665563718 2213 5 -0.0726231805777644 2213 6 -0.37346775226743034 2213 7 -0.36493728291188887 2213 8 -0.3946141217731878 2213 9 -0.32995117098147375 2213 10 -0.30776918743195836 2213 11 -0.12072852216423513 2213 12 0.021940420723950638 2213 13 -0.04715911875903345 2213 14 -0.7449745422323559 2213 15 -0.41517233980874835 2213 16 -0.5457645911373135 2213 17 -0.35424725321433215 2213 18 -0.362495154640321 2213 19 -0.5126988279090341 2213 20 -0.3649060252347951 2213 21 -0.030112465358179463 2213 22 -0.43120828159804603 2213 23 -0.6402398119789723 2213 24 -0.6932245232614989 2213 25 -0.5469347432664335 2214 1 -0.285511590142697 2214 2 -0.15276047839253337 2214 3 -0.1371436710824554 2214 4 -0.12419530625787445 2214 5 -0.16085741323635716 2214 6 -0.12099700220045406 2214 7 -0.3309405254243387 2214 8 -0.26151703656423564 2214 9 -0.34518098543924935 2214 10 -0.29892168129325825 2214 11 -0.14466460746896814 2214 12 -0.44995861443426577 2214 13 -0.4350710434594518 2214 14 -0.37579277692307045 2214 15 -0.08892714423777903 2214 16 -0.5154434213898469 2214 17 -0.5175468288838303 2214 18 -0.4272947393322184 2214 19 -0.5998763984954536 2214 20 -0.5786928800116016 2214 21 -0.43710815820229953 2214 22 -0.8538585358215727 2214 23 -1.1791745994458793 2214 24 -0.7072501940770834 2214 25 -1.2347318752530754 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 18.0 10 60.0 11 167.0 12 491.0 13 1178.0 14 2586.0 15 4692.0 16 7083.0 17 9327.0 18 11079.0 19 12182.0 20 12943.0 21 13807.0 22 14506.0 23 15053.0 24 16657.0 25 18262.0 26 20566.0 27 22825.0 28 26956.0 29 33745.0 30 43108.0 31 56665.0 32 76228.0 33 102624.0 34 141965.0 35 199317.0 36 289248.0 37 468370.0 38 998796.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.122859958593395 21.259119765922822 12.35765525565903 22.26036501982475 2 13.677190194729343 22.7534040142583 40.129150481833875 23.440255309178486 3 19.249905523647364 30.03881841320231 28.45150105633924 22.259775006811086 4 11.420023540223708 18.48300661477233 36.99921685247974 33.09775299252422 5 12.260052040332976 39.74165337412892 33.248596983614966 14.749697601923135 6 30.34402268269681 37.18886764750222 17.749805640675888 14.717304029125083 7 24.36416337205441 35.15145801382783 22.989892111786023 17.49448650233174 8 25.596548078427777 36.28849728434078 20.980194580895347 17.13476005633609 9 26.23514407915424 16.339872024618167 22.208094320787147 35.216889575440455 10 16.299142247198933 31.03553306493757 31.114534595927474 21.55079009193602 11 30.430132204144495 28.03162421198181 20.402420541379808 21.13582304249389 12 22.06913263651177 30.50380275294127 30.48930153751045 16.937763073036514 13 27.267565194395438 24.55431368486046 26.31744443541312 21.86067668533098 14 21.364398368085862 24.18235430823578 28.734228563382064 25.719018760296297 15 26.392443176137377 27.478280580629555 24.949837166366592 21.17943907686648 16 20.166738662885212 31.342663330926673 28.07478549960542 20.415812506582693 17 19.74087457121847 30.377747512218058 29.77866496826759 20.102712948295885 18 20.065071576062422 28.44805512232594 31.906556728317774 19.580316573293864 19 19.933554436935673 28.2259698659526 33.3811487999768 18.459326897134925 20 22.654479707354227 26.202038842879965 31.448582830949444 19.694898618816367 21 21.932744184129604 28.550102232320373 30.663437768793916 18.853715814756104 22 20.29820421848567 27.897027154817604 30.73722179544569 21.067546831251033 23 18.726536020142674 28.781540664283924 31.850871549235148 20.641051766338254 24 19.47194971356699 29.036278462038656 31.142129693937203 20.34964213045715 25 20.778832724025552 26.73530601497447 29.369957946314766 23.11590331468521 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2918.0 1 2918.0 2 3107.5 3 3297.0 4 3297.0 5 3297.0 6 6348.5 7 9400.0 8 9400.0 9 9400.0 10 11797.0 11 14194.0 12 14194.0 13 14194.0 14 17659.5 15 21125.0 16 21125.0 17 21125.0 18 34217.0 19 47309.0 20 47309.0 21 47309.0 22 71001.5 23 94694.0 24 94694.0 25 94694.0 26 125345.5 27 155997.0 28 155997.0 29 155997.0 30 186814.0 31 217631.0 32 217631.0 33 217631.0 34 238317.0 35 259003.0 36 259003.0 37 259003.0 38 272031.5 39 285060.0 40 285060.0 41 285060.0 42 290295.5 43 295531.0 44 295531.0 45 295531.0 46 364491.5 47 433452.0 48 433452.0 49 433452.0 50 386497.0 51 339542.0 52 339542.0 53 339542.0 54 269981.5 55 200421.0 56 200421.0 57 200421.0 58 160451.5 59 120482.0 60 120482.0 61 120482.0 62 96390.5 63 72299.0 64 72299.0 65 72299.0 66 51768.5 67 31238.0 68 31238.0 69 31238.0 70 21552.0 71 11866.0 72 11866.0 73 11866.0 74 7817.0 75 3768.0 76 3768.0 77 3768.0 78 2386.5 79 1005.0 80 1005.0 81 1005.0 82 611.0 83 217.0 84 217.0 85 217.0 86 129.0 87 41.0 88 41.0 89 41.0 90 28.5 91 16.0 92 16.0 93 16.0 94 9.0 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09803438950219212 2 0.0 3 0.13173013334432357 4 0.011944244419690983 5 0.02358320463696175 6 0.01381411015951481 7 0.021942302048953085 8 0.019805312632011567 9 1.9080262651263553E-4 10 0.39812876048126533 11 0.0670098824312376 12 0.0014882604867985571 13 0.0 14 0.001068494708470759 15 0.0016409025880086657 16 3.8160525302527104E-5 17 7.632105060505421E-5 18 3.8160525302527107E-4 19 6.868894554454878E-4 20 1.1448157590758131E-4 21 2.6712367711768974E-4 22 0.0034344472772274395 23 1.1448157590758131E-4 24 3.8160525302527104E-5 25 0.001869865739823828 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2620509.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.65877206773881 #Duplication Level Percentage of deduplicated Percentage of total 1 87.25541886668418 54.67317402442641 2 9.24420783256225 11.584614230546476 3 1.8941744322537302 3.560599320213752 4 0.6037008616077212 1.5130861873830292 5 0.2631595474820279 0.8244627051562838 6 0.14221596052613358 0.5346646473000927 7 0.09349222063500362 0.4100675420012807 8 0.06629521929980926 0.3323181628230006 9 0.049344487070109216 0.27826784713129127 >10 0.3116186535853608 3.7684937830837075 >50 0.041022456094836075 1.7805902832716543 >100 0.027486315449018653 3.5686589600915726 >500 0.0035015575369988935 1.47311991677794 >1k 0.003132972533102526 4.41593397957619 >5k 5.528775058416222E-4 2.3845673968371477 >10k+ 6.757391738064271E-4 8.897381013380254 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 39627 1.5121871361632415 No Hit GTACATGGGAAGCAGTGGTATCAAC 38748 1.4786440344223202 No Hit GTATCAACGCAGAGTACTTTTTTTT 32801 1.2517033904481916 No Hit GGTATCAACGCAGAGTACTTTTTTT 21351 0.8147653757342561 No Hit TATCAACGCAGAGTACTTTTTTTTT 19947 0.7611879982095082 No Hit GAGTACATGGGAAGCAGTGGTATCA 18406 0.7023826287183139 No Hit CATGTACTCTGCGTTGATACCACTG 14027 0.5352776884185476 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 11932 0.4553313879097534 No Hit GCGTTGATACCACTGCTTCCCATGT 11750 0.44838617230469346 No Hit ACGCAGAGTACATGGGAAGCAGTGG 11297 0.43109945434264874 No Hit GTATCAACGCAGAGTACATGGGAAG 11264 0.4298401570076653 No Hit TATCAACGCAGAGTACATGGGAAGC 8748 0.3338282753465071 No Hit GGTATCAACGCAGAGTACATGGGAA 8325 0.31768637314353815 No Hit ACTCTGCGTTGATACCACTGCTTCC 8084 0.3084896865456291 No Hit GCTTCCCATGTACTCTGCGTTGATA 7909 0.30181159461768686 No Hit GTGGTATCAACGCAGAGTACATGGG 7061 0.2694514691611439 No Hit CAGTGGTATCAACGCAGAGTACATG 6055 0.2310619807068016 No Hit ATTCCATTCCATTCCATTCCATTCC 5421 0.20686820766499942 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 5226 0.19942690523100665 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 5121 0.1954200500742413 No Hit CATGGGAAGCAGTGGTATCAACGCA 4964 0.18942884760174455 No Hit ACATGGGAAGCAGTGGTATCAACGC 4504 0.17187500596258207 No Hit GCAGTGGTATCAACGCAGAGTACAT 4498 0.1716460428107669 No Hit GAATGGAATGGAATGGAATGGAATG 4340 0.16561667981296765 No Hit GGGAAGCAGTGGTATCAACGCAGAG 4233 0.16153350360559723 No Hit ATACCACTGCTTCCCATGTACTCTG 4031 0.15382507749448676 No Hit ATCAACGCAGAGTACTTTTTTTTTT 3484 0.13295127015400443 No Hit GCAGAGTACATGGGAAGCAGTGGTA 3412 0.1302037123322225 No Hit CTGCTTCCCATGTACTCTGCGTTGA 3409 0.1300892307563149 No Hit GTACTCTGCGTTGATACCACTGCTT 3374 0.12875361237072644 No Hit CCATGTACTCTGCGTTGATACCACT 3264 0.12455595458744848 No Hit GTGGTATCAACGCAGAGTACTTTTT 3180 0.12135047046203619 No Hit CTTCCCATGTACTCTGCGTTGATAC 3121 0.11909899946918709 No Hit GATACCACTGCTTCCCATGTACTCT 3108 0.11860291264025424 No Hit AAGCAGTGGTATCAACGCAGAGTAC 3078 0.11745809688117842 No Hit TACCACTGCTTCCCATGTACTCTGC 3029 0.1155882311413546 No Hit ATGGGAAGCAGTGGTATCAACGCAG 3005 0.11467237853409395 No Hit GTACATGGTAAGCAGTGGTATCAAC 2820 0.10761268135312645 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1448157590758131E-4 0.0 10 0.0 0.0 0.0 1.9080262651263553E-4 0.0 11 0.0 0.0 0.0 1.9080262651263553E-4 0.0 12 0.0 0.0 0.0 2.2896315181516262E-4 0.0 13 0.0 3.8160525302527104E-5 0.0 2.2896315181516262E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACGTT 30 7.613125E-4 19.04113 6 TATCGCG 175 0.0 13.056775 7 CGCGCCT 185 0.0 12.813833 12 TCGCGCG 180 0.0 12.693601 9 GCGCCTT 190 0.0 12.476626 13 TGTATCG 210 0.0 12.240726 5 GCGCGCC 195 0.0 12.166232 11 ATCGCGC 195 0.0 11.71762 8 CGCGCGC 215 0.0 11.5128 10 CGATAAG 75 2.111228E-4 11.378683 12 CCCCGTA 195 0.0 11.184176 15 CCCGTAC 195 0.0 11.184176 16 GTATCGC 225 0.0 11.001543 6 CGCCTTT 225 0.0 10.957251 14 AGCGTCG 80 3.838337E-4 10.667516 17 CCGTACA 215 1.8189894E-12 10.143787 17 CACGGTA 150 2.4454494E-8 10.13372 2 GCTACAC 150 2.4454494E-8 10.13372 3 CGTCGGT 75 0.002690046 10.114385 19 CGCAGCG 180 3.783498E-10 10.049485 8 >>END_MODULE