FastQCFastQC Report
Wed 25 May 2016
SRR1294598_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294598_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3552459
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT136820.38514167228953244No Hit
GTACATGGGAAGCAGTGGTATCAAC120250.3384979249584584No Hit
CCCATGTACTCTGCGTTGATACCAC113550.3196377495137875No Hit
GGTATCAACGCAGAGTACTTTTTTT75630.2128947864000682No Hit
TATCAACGCAGAGTACTTTTTTTTT73410.2066455939392967No Hit
GAGTACATGGGAAGCAGTGGTATCA51790.14578634123574685No Hit
CATGTACTCTGCGTTGATACCACTG39590.11144393221709244No Hit
GTATCAACGCAGAGTACATGGGAAG37640.10595477667722555No Hit
GCGTTGATACCACTGCTTCCCATGT37140.10454730089777249No Hit
ACGCAGAGTACTTTTTTTTTTTTTT36100.10161975127651016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCTA4500.010.77073116
GTACCGT1158.8575507E-710.7362256
CCCGCCC4600.010.330132514
TGTCCCG5050.09.59078811
CCCCGTA4400.09.50372115
TCTAGAC2900.09.4979653
GTCCCGC5300.09.3238812
CCGTACA4600.09.2972517
GGCGCGA1451.6988361E-69.17600715
CCGCCCT5200.09.13819315
GTCTCGC2401.0913936E-119.1051661
ACCGGGG2201.6734703E-109.07173315
CGCCTTT2950.09.02048114
CGTTCCG950.00182939168.99743810
GTTCTAG3400.08.9421581
CTGCGCA850.0074560758.9386319
CTAGACA4800.08.9039664
AGTACCG1401.08967815E-58.8190415
GTCTTAC3450.08.8125611
TCCCGCC5400.08.79974213
Produced by FastQC (version 0.11.5)