##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294598_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3552459 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.887130857808632 31.0 30.0 33.0 25.0 34.0 2 29.989366520486232 31.0 30.0 34.0 25.0 34.0 3 30.023909635551036 31.0 30.0 34.0 25.0 34.0 4 33.62129640342084 35.0 35.0 37.0 27.0 37.0 5 34.062118099040696 35.0 35.0 37.0 28.0 37.0 6 34.06244378893606 37.0 35.0 37.0 29.0 37.0 7 34.001101490545 37.0 35.0 37.0 29.0 37.0 8 33.8007495653011 37.0 35.0 37.0 28.0 37.0 9 34.9264413748336 39.0 35.0 39.0 27.0 39.0 10 35.08130762381776 39.0 35.0 39.0 27.0 39.0 11 35.282117541680286 39.0 35.0 39.0 27.0 39.0 12 35.08853219699369 39.0 35.0 39.0 27.0 39.0 13 34.91881341909928 38.0 35.0 39.0 27.0 39.0 14 35.80273523213076 39.0 35.0 41.0 27.0 41.0 15 35.78723104193462 39.0 34.0 41.0 27.0 41.0 16 35.92147664476916 39.0 35.0 41.0 27.0 41.0 17 35.92008436972812 39.0 35.0 41.0 27.0 41.0 18 35.824506067487334 39.0 35.0 41.0 27.0 41.0 19 36.10710581036966 39.0 35.0 41.0 27.0 41.0 20 36.21737365582544 39.0 35.0 41.0 27.0 41.0 21 36.26936975205062 39.0 35.0 41.0 27.0 41.0 22 36.28769846464097 39.0 35.0 41.0 27.0 41.0 23 36.282339641358284 39.0 35.0 41.0 27.0 41.0 24 36.30100727411632 39.0 35.0 41.0 27.0 41.0 25 35.12317636881946 38.0 33.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0192029794121105 1101 2 0.993042585550377 1101 3 1.3270548892750789 1101 4 1.1118886330142175 1101 5 0.8337243133292347 1101 6 0.8461063434685485 1101 7 0.9174848526299328 1101 8 1.0226108303689045 1101 9 1.2731514082229936 1101 10 1.2545723024467321 1101 11 0.9971555176258917 1101 12 1.0903214106823569 1101 13 1.2713364779814924 1101 14 1.402978960673245 1101 15 1.512649332986868 1101 16 1.3820087817360545 1101 17 1.429295340019408 1101 18 1.4309045511028913 1101 19 1.3366124976615552 1101 20 1.2053471788310972 1101 21 1.0596036685502384 1101 22 1.0386532307456804 1101 23 1.0710332028396934 1101 24 1.0571673292887027 1101 25 1.1202233540021211 1102 1 0.9943353863303912 1102 2 0.9161118775158812 1102 3 1.2536876950487645 1102 4 1.1074266833335642 1102 5 0.8732197878485621 1102 6 0.797889332064905 1102 7 0.6484032784116494 1102 8 0.866689477417296 1102 9 1.2299524023355062 1102 10 1.0480329593066955 1102 11 0.8592986804109017 1102 12 1.0094829232491165 1102 13 1.0782614472424044 1102 14 1.2751752869457889 1102 15 1.4147682390373362 1102 16 1.2634858027681943 1102 17 1.3093177476949478 1102 18 1.3647220459381444 1102 19 1.0470454785484336 1102 20 0.3604193636007551 1102 21 0.6461495746332488 1102 22 0.7078045704467115 1102 23 0.8742020885292234 1102 24 0.9061428198291566 1102 25 0.9644278465464282 1103 1 0.7256544848611775 1103 2 0.7706992356523124 1103 3 0.9955615950834513 1103 4 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0.271219338953685 2207 7 0.2872162970923924 2207 8 0.2917513207194631 2207 9 -1.3151198761685308 2207 10 -0.15347518814338912 2207 11 0.11345498926381481 2207 12 0.4207972341371118 2207 13 0.5251651315435097 2207 14 0.7250079345358671 2207 15 0.7546926306050423 2207 16 0.6849710911142992 2207 17 0.7295580579321879 2207 18 0.8003318637471253 2207 19 -0.4656667054654662 2207 20 0.23271725696202594 2207 21 -0.40856064397149083 2207 22 0.27016866722096466 2207 23 -0.4625921605239256 2207 24 -1.50783459757384 2207 25 -0.12527153224851162 2208 1 0.43494613824622874 2208 2 0.1802058533081734 2208 3 0.6361117815710635 2208 4 0.5146279399291487 2208 5 0.39966835309235194 2208 6 0.26601370567026805 2208 7 0.32688781995105387 2208 8 0.471947884837256 2208 9 -0.2169004734415978 2208 10 -0.031652751657084366 2208 11 0.1305047107586006 2208 12 0.23423346691126312 2208 13 0.5475471931696774 2208 14 0.780022374506899 2208 15 0.6050884103489551 2208 16 0.34885901966264043 2208 17 0.3889693146212423 2208 18 0.668264320591355 2208 19 -1.4798113306217928 2208 20 -0.1733135202901579 2208 21 -0.3142268339864387 2208 22 0.15524110143150693 2208 23 0.3294121183016401 2208 24 0.45153437830673226 2208 25 0.3842063414083725 2209 1 0.1711380835713605 2209 2 -1.3580434102354992 2209 3 -0.20768091807603994 2209 4 0.1796962410587568 2209 5 0.11670709797462564 2209 6 0.23431210109635003 2209 7 0.07737652533572259 2209 8 0.2770837646031481 2209 9 -2.029806019066605 2209 10 -0.3894583162811287 2209 11 0.0036388182152862214 2209 12 0.2757692766011459 2209 13 0.48223706904805397 2209 14 0.5697676665963414 2209 15 0.7930265655442597 2209 16 0.6097533035837372 2209 17 0.43994193862894093 2209 18 0.7041381585358337 2209 19 0.35565104056310304 2209 20 0.38301692719844027 2209 21 0.4489415706758706 2209 22 0.340065758340657 2209 23 0.44243398729798145 2209 24 0.4923389312843369 2209 25 0.5346662950644188 2210 1 0.16616420682177946 2210 2 0.17454629784539577 2210 3 0.07971025759997374 2210 4 0.2644092323512197 2210 5 0.19772065525625493 2210 6 0.1403304461945254 2210 7 0.15195749388036717 2210 8 0.2608931042760858 2210 9 0.4115209010070302 2210 10 -1.450607051573222 2210 11 -0.3932139824146219 2210 12 -2.235626920750178 2210 13 -0.7960141926988129 2210 14 -0.5370939709977023 2210 15 -0.8417704891406501 2210 16 -0.04121395475569756 2210 17 0.15077369897417014 2210 18 0.3999150814670287 2210 19 0.3295259991920716 2210 20 0.2512172128277186 2210 21 0.31468705560169496 2210 22 0.2870571071581267 2210 23 0.1442494891256345 2210 24 0.14142923022080112 2210 25 0.11553673523629726 2211 1 -0.3240482889647218 2211 2 -0.0835942184962164 2211 3 -1.6488172205135179 2211 4 -0.7143381430950058 2211 5 -0.5693943628093407 2211 6 -0.49038663063129206 2211 7 -0.42718955490959587 2211 8 -0.07241008025017948 2211 9 0.09641995298638761 2211 10 -0.07181659350990088 2211 11 -0.2933192522343191 2211 12 -0.40238262369739886 2211 13 -0.41234013554141313 2211 14 -0.8198509742074123 2211 15 -0.3825493030521727 2211 16 -0.1854962179840598 2211 17 -0.24172146480746193 2211 18 -0.10133263918162783 2211 19 -0.09286428735302366 2211 20 -0.06851376279557542 2211 21 -0.2876052239120952 2211 22 -0.21696509341938253 2211 23 -0.6004213637409705 2211 24 -0.31251661970142663 2211 25 -0.2203409994776706 2212 1 -0.3562227122865842 2212 2 -0.3438301474098786 2212 3 -2.4332835019863666 2212 4 -1.37003186160009 2212 5 -0.5716096272314886 2212 6 -0.31695813073923773 2212 7 -0.2670317690697104 2212 8 -0.16266344550093947 2212 9 -0.8393084837181348 2212 10 -0.22328666899288407 2212 11 -0.28365816707128744 2212 12 0.06828273673816199 2212 13 -0.34020084235165626 2212 14 -0.052132444851139326 2212 15 0.13249692811340452 2212 16 -1.04231619532041 2212 17 -1.2743887361324227 2212 18 -0.1734067633373968 2212 19 -0.16331228894631522 2212 20 -0.08629217410371837 2212 21 0.068151774069797 2212 22 -0.17527711751302633 2212 23 0.06354176497585229 2212 24 -0.14335617432130476 2212 25 0.06676020281400241 2213 1 -2.9149855958655557 2213 2 -1.3351974494291206 2213 3 -2.328736395225391 2213 4 -1.008060991108124 2213 5 -0.9147977522843362 2213 6 -0.6139861361084584 2213 7 -0.35629622501543423 2213 8 -0.22773567260992422 2213 9 -0.3130792977652206 2213 10 -0.26457576448025577 2213 11 -0.32326072488061186 2213 12 -0.19293418105694116 2213 13 -0.5352306186696225 2213 14 -0.11292708763964754 2213 15 -0.2188360970060259 2213 16 -1.3975538732617707 2213 17 -0.9277730675187428 2213 18 -0.23167127016403555 2213 19 -0.14630612942695365 2213 20 -0.19982580254637128 2213 21 0.014979824755975812 2213 22 0.039381857716023205 2213 23 0.09629238369146265 2213 24 0.19477856054335518 2213 25 0.09417916031159734 2214 1 -1.0436731162998036 2214 2 -0.758532870502691 2214 3 -6.4711919716583495 2214 4 -2.170651049525464 2214 5 -1.4502842154554187 2214 6 -0.792794390954576 2214 7 -0.7271017382253149 2214 8 -0.5593365754590991 2214 9 -0.3876872556007456 2214 10 -0.33561959520785223 2214 11 -1.0171538877282913 2214 12 -0.6886409205187789 2214 13 -5.302034761061389 2214 14 -4.220001375739923 2214 15 -2.3183385845682167 2214 16 -3.05002154283158 2214 17 -3.4971675834584417 2214 18 -1.3579606467175367 2214 19 -0.8623450953258853 2214 20 -0.489559241448454 2214 21 -0.5184110199122074 2214 22 -0.4900840353050171 2214 23 -0.4991634330285777 2214 24 -0.3431643456698339 2214 25 -0.349191476606741 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1404.0 3 195.0 4 170.0 5 350.0 6 62.0 7 51.0 8 112.0 9 286.0 10 791.0 11 2007.0 12 4404.0 13 8200.0 14 13064.0 15 18623.0 16 23898.0 17 27480.0 18 28522.0 19 28446.0 20 28195.0 21 28257.0 22 27916.0 23 28789.0 24 29910.0 25 32449.0 26 35191.0 27 40369.0 28 46658.0 29 56938.0 30 70676.0 31 87867.0 32 115308.0 33 152873.0 34 206329.0 35 280273.0 36 393876.0 37 592586.0 38 1139934.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.401159148796594 21.543159103147506 13.568403614619093 22.487278133436806 2 14.67210099720773 22.20464417264655 41.08357268834879 22.039682141796934 3 18.12867918363668 28.464079901306338 29.352745339935442 24.054495575121535 4 11.759654524925303 18.435448315758315 38.57224815375703 31.23264900555936 5 12.484341027634144 39.45396374778915 34.550931089256146 13.510764135320558 6 29.248320998212407 39.68075163393169 18.045952339503994 13.024975028351903 7 24.849809078382084 34.5600239764652 23.576622721080923 17.013544224071786 8 23.350833297420802 37.87771882489699 21.73291049499295 17.03853738268926 9 24.29446294002671 18.335358160636176 22.13342339660015 35.23675550273697 10 14.663586196134371 31.154075074356914 32.977794753791926 21.20454397571679 11 30.904082919685987 27.86571490457076 22.981094417500135 18.249107758243113 12 20.568753057846212 29.056080723478317 31.53745338764998 18.837712831025495 13 25.749026694841014 25.508087544792318 26.99918372497447 21.743702035392197 14 19.99149905610792 24.61614773727858 28.608070432207366 26.784282774406133 15 22.69674473500005 31.160940763557033 24.704437486884153 21.437877014558765 16 20.776774998218432 32.26229866518996 26.247076608558906 20.713849728032702 17 19.37179485012729 30.88256088622834 28.955344371752602 20.79029989189177 18 19.759994925896954 30.327462878003058 29.93072439868355 19.981817797416433 19 20.277394440449097 29.50574414437559 30.339161281399267 19.87770013377604 20 22.00778552073416 28.692516999138075 29.21682844250151 20.08286903762626 21 22.118326975120723 29.359253283699466 28.6475315638499 19.87488817732991 22 20.93916854809292 28.948706428491956 29.551964687871035 20.560160335544097 23 20.620270026272507 29.47036124908066 29.351716670436517 20.55765205421032 24 20.746600993770205 28.78631912317443 29.377070941489936 21.090008941565433 25 21.18490307732828 28.678350251197536 28.719082702933385 21.4176639685408 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3704.0 1 3704.0 2 3821.5 3 3939.0 4 3939.0 5 3939.0 6 6479.0 7 9019.0 8 9019.0 9 9019.0 10 13322.0 11 17625.0 12 17625.0 13 17625.0 14 27844.5 15 38064.0 16 38064.0 17 38064.0 18 59761.5 19 81459.0 20 81459.0 21 81459.0 22 115568.0 23 149677.0 24 149677.0 25 149677.0 26 194671.0 27 239665.0 28 239665.0 29 239665.0 30 283903.5 31 328142.0 32 328142.0 33 328142.0 34 360488.0 35 392834.0 36 392834.0 37 392834.0 38 407368.0 39 421902.0 40 421902.0 41 421902.0 42 415831.0 43 409760.0 44 409760.0 45 409760.0 46 419092.5 47 428425.0 48 428425.0 49 428425.0 50 393404.5 51 358384.0 52 358384.0 53 358384.0 54 311763.5 55 265143.0 56 265143.0 57 265143.0 58 224209.0 59 183275.0 60 183275.0 61 183275.0 62 151061.0 63 118847.0 64 118847.0 65 118847.0 66 89301.5 67 59756.0 68 59756.0 69 59756.0 70 43321.0 71 26886.0 72 26886.0 73 26886.0 74 18714.0 75 10542.0 76 10542.0 77 10542.0 78 7150.0 79 3758.0 80 3758.0 81 3758.0 82 2467.5 83 1177.0 84 1177.0 85 1177.0 86 743.5 87 310.0 88 310.0 89 310.0 90 213.5 91 117.0 92 117.0 93 117.0 94 71.0 95 25.0 96 25.0 97 25.0 98 24.5 99 24.0 100 24.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10268943286889448 2 0.05475080782072362 3 0.058522842909657785 4 0.05263959415154404 5 0.05007798823293949 6 0.06848777142818538 7 0.06505353052631994 8 0.06097185076590609 9 0.06648915582136204 10 0.06620766066547143 11 0.060014767235878015 12 0.06097185076590609 13 0.06150669156209825 14 0.06375865280922313 15 0.06654545485254018 16 0.062379326545359146 17 0.06510982955749807 18 0.141704661475333 19 0.07026119091029621 20 0.06415274602747 21 0.06178818671798886 22 0.06767143547610262 23 0.06643285679018392 24 0.0772704202919724 25 0.06936040641144627 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3552459.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.3617840553082 #Duplication Level Percentage of deduplicated Percentage of total 1 87.3139518900143 65.80135187350827 2 9.324528371928164 14.054261871656898 3 1.9017673197523237 4.299617342238507 4 0.5783865641742504 1.7435297339916607 5 0.2660196679700712 1.0023858386012647 6 0.1457154369655512 0.6588825176473645 7 0.09473772126102697 0.49977225840958467 8 0.06038337049366623 0.3640478822140275 9 0.04368490673192493 0.2962955256846846 >10 0.23302797483421173 3.1893878981591683 >50 0.019097435733902827 1.0135192096932393 >100 0.01488718643670083 2.158218325552315 >500 0.0020004371051303097 1.0603587034081783 >1k 0.0015852520239149957 2.232902982122021 >5k 1.1323228742249966E-4 0.5712533833115483 >10k+ 1.1323228742249966E-4 1.0542146538013548 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 13682 0.38514167228953244 No Hit GTACATGGGAAGCAGTGGTATCAAC 12025 0.3384979249584584 No Hit CCCATGTACTCTGCGTTGATACCAC 11355 0.3196377495137875 No Hit GGTATCAACGCAGAGTACTTTTTTT 7563 0.2128947864000682 No Hit TATCAACGCAGAGTACTTTTTTTTT 7341 0.2066455939392967 No Hit GAGTACATGGGAAGCAGTGGTATCA 5179 0.14578634123574685 No Hit CATGTACTCTGCGTTGATACCACTG 3959 0.11144393221709244 No Hit GTATCAACGCAGAGTACATGGGAAG 3764 0.10595477667722555 No Hit GCGTTGATACCACTGCTTCCCATGT 3714 0.10454730089777249 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3610 0.10161975127651016 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.8149515589060986E-5 0.0 5 0.0 0.0 0.0 2.8149515589060986E-5 0.0 6 0.0 0.0 0.0 2.8149515589060986E-5 0.0 7 0.0 0.0 0.0 8.444854676718295E-5 0.0 8 0.0 0.0 0.0 1.1259806235624394E-4 0.0 9 0.0 0.0 0.0 1.1259806235624394E-4 0.0 10 0.0 0.0 0.0 1.1259806235624394E-4 0.0 11 0.0 0.0 0.0 1.1259806235624394E-4 0.0 12 0.0 0.0 0.0 1.688970935343659E-4 0.0 13 0.0 0.0 0.0 2.2519612471248789E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCCCTA 450 0.0 10.770731 16 GTACCGT 115 8.8575507E-7 10.736225 6 CCCGCCC 460 0.0 10.3301325 14 TGTCCCG 505 0.0 9.590788 11 CCCCGTA 440 0.0 9.503721 15 TCTAGAC 290 0.0 9.497965 3 GTCCCGC 530 0.0 9.32388 12 CCGTACA 460 0.0 9.29725 17 GGCGCGA 145 1.6988361E-6 9.176007 15 CCGCCCT 520 0.0 9.138193 15 GTCTCGC 240 1.0913936E-11 9.105166 1 ACCGGGG 220 1.6734703E-10 9.071733 15 CGCCTTT 295 0.0 9.020481 14 CGTTCCG 95 0.0018293916 8.997438 10 GTTCTAG 340 0.0 8.942158 1 CTGCGCA 85 0.007456075 8.938631 9 CTAGACA 480 0.0 8.903966 4 AGTACCG 140 1.08967815E-5 8.819041 5 GTCTTAC 345 0.0 8.812561 1 TCCCGCC 540 0.0 8.799742 13 >>END_MODULE