FastQCFastQC Report
Wed 25 May 2016
SRR1294598_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294598_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3552459
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT356861.0045436133112304No Hit
GGTATCAACGCAGAGTACTTTTTTT217300.6116889737502952No Hit
TATCAACGCAGAGTACTTTTTTTTT213080.5998098781717115No Hit
CCCATGTACTCTGCGTTGATACCAC132160.37202399802503No Hit
ACGCAGAGTACTTTTTTTTTTTTTT123810.34851915250816407No Hit
GTACATGGGAAGCAGTGGTATCAAC113450.3193562543578969No Hit
GTACTTTTTTTTTTTTTTTTTTTTT65590.184632672748651No Hit
GAGTACTTTTTTTTTTTTTTTTTTT61380.17278172668565633No Hit
GAGTACATGGGAAGCAGTGGTATCA54610.15372450463186205No Hit
CATGTACTCTGCGTTGATACCACTG46030.1295722202564477No Hit
ATCAACGCAGAGTACTTTTTTTTTT42370.11926949755085141No Hit
GCGTTGATACCACTGCTTCCCATGT40340.11355514588627202No Hit
GCAGAGTACTTTTTTTTTTTTTTTT39030.10986755934410504No Hit
GTATCAACGCAGAGTACATGGGAAG36980.10409690864834752No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGA350.002135696616.3256656
GACCGTG751.4430356E-512.6977397
CTGCTCG550.003016195312.11936959
AGACCGT657.864549E-411.7209896
ACCGTTC657.864549E-411.7209898
GGAGCGT600.005771945711.110526
TACCGTC700.001462027410.8837777
CCCCGGT3350.010.75572319
TCGCGCG3300.010.6765879
CGCGCCT3550.010.41671912
TATCGCG3400.010.3635957
ACACCGT750.002595702210.1581916
ATACCGT856.450436E-410.0834986
CAGTGCG856.4563076E-410.08259
CCCCCGG3600.010.00851518
CGCCTTT4000.09.95597914
GCGCGCC3650.09.8775311
CTAGGAC3200.09.7933573
GCGCCTT3600.09.74527113
ATCGCGC3750.09.6502828
Produced by FastQC (version 0.11.5)