##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294598_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3552459 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.19612161604117 31.0 31.0 34.0 28.0 34.0 2 31.41911053723632 31.0 31.0 34.0 28.0 34.0 3 31.48423190809521 31.0 31.0 34.0 28.0 34.0 4 35.08771192010942 37.0 35.0 37.0 32.0 37.0 5 35.300418667745355 37.0 35.0 37.0 32.0 37.0 6 34.80405628889735 37.0 35.0 37.0 32.0 37.0 7 34.66758715582643 37.0 35.0 37.0 31.0 37.0 8 34.674107146627165 37.0 35.0 37.0 31.0 37.0 9 36.13323475372974 39.0 35.0 39.0 30.0 39.0 10 35.91168511726666 39.0 35.0 39.0 30.0 39.0 11 36.07506715770682 39.0 35.0 39.0 30.0 39.0 12 36.05265479488996 39.0 35.0 39.0 30.0 39.0 13 35.88100439723583 39.0 35.0 39.0 30.0 39.0 14 36.950273599216764 40.0 36.0 41.0 30.0 41.0 15 36.97986859243133 40.0 36.0 41.0 30.0 41.0 16 37.0413837288481 40.0 36.0 41.0 30.0 41.0 17 37.107710461964516 40.0 36.0 41.0 31.0 41.0 18 37.129435976600995 40.0 36.0 41.0 31.0 41.0 19 37.17160310646794 40.0 37.0 41.0 30.0 41.0 20 37.229443323624565 40.0 37.0 41.0 31.0 41.0 21 37.086060388029814 40.0 37.0 41.0 30.0 41.0 22 37.08601365983394 40.0 36.0 41.0 30.0 41.0 23 37.095220803392806 40.0 36.0 41.0 30.0 41.0 24 37.026613959513675 40.0 36.0 41.0 30.0 41.0 25 35.94333699558531 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4746789543940757 1101 2 0.47996798058072443 1101 3 0.3946546356041445 1101 4 0.35211621732301523 1101 5 0.3969664060329592 1101 6 0.43439175077249104 1101 7 0.5349679359532615 1101 8 0.522979529197066 1101 9 0.667556693824487 1101 10 0.7408975230544144 1101 11 0.751437259356976 1101 12 0.7953939173328806 1101 13 0.7785223897938351 1101 14 0.9854631266740412 1101 15 0.9636766468231954 1101 16 1.0061010745144259 1101 17 0.9902189491463247 1101 18 1.0183493268734338 1101 19 1.0874638591040053 1101 20 1.0154115545536797 1101 21 1.3259166987413664 1101 22 1.2436850940072972 1101 23 1.185044814907819 1101 24 1.239843621786811 1101 25 1.2177551328751548 1102 1 0.3931523007255642 1102 2 0.39538776411308874 1102 3 0.3948724474557004 1102 4 0.3304441509782947 1102 5 0.3496414439493094 1102 6 0.49461371692436984 1102 7 0.5119180773545722 1102 8 0.604456306931958 1102 9 0.7089580980753212 1102 10 0.7820849473851723 1102 11 0.6887593292447178 1102 12 0.7492424386483023 1102 13 0.771291474257211 1102 14 1.0025023270290134 1102 15 0.9850843707197612 1102 16 0.9341921431032603 1102 17 0.898982424391896 1102 18 0.9134472220786236 1102 19 1.0512572158121216 1102 20 0.9418477761597543 1102 21 1.261013997868325 1102 22 1.1682777307988914 1102 23 1.0417729110856513 1102 24 1.10470678685882 1102 25 0.992824343336089 1103 1 0.3080252147587501 1103 2 0.30865790184603625 1103 3 0.24009065499937776 1103 4 0.20639524296509393 1103 5 0.24936160972742272 1103 6 0.26936463711373193 1103 7 0.2691842395170383 1103 8 0.3480091141700896 1103 9 0.25708528910032413 1103 10 0.561118557972776 1103 11 0.5317557573683658 1103 12 0.4557007290634516 1103 13 0.6713108074997365 1103 14 0.7816314078953468 1103 15 0.7517493262810007 1103 16 0.7100754184828659 1103 17 0.5956711614564654 1103 18 0.6743787683146465 1103 19 0.7955105541082759 1103 20 0.6993502834811665 1103 21 0.9524042980103218 1103 22 0.7972808409834329 1103 23 0.7931153775515085 1103 24 0.9472765425655041 1103 25 0.8003873148266365 1104 1 0.18201872277234088 1104 2 0.1723372251110078 1104 3 0.0593997619620481 1104 4 0.10169155848152656 1104 5 0.104965916069375 1104 6 0.08979537963647033 1104 7 0.18097110077615497 1104 8 0.07388759978030635 1104 9 -0.04554214066040174 1104 10 0.29545596474233804 1104 11 0.3675187471370833 1104 12 0.3613363209745657 1104 13 -0.10377853045461904 1104 14 0.28603826415582745 1104 15 0.43363076971004944 1104 16 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0.18191716771014654 2207 5 0.20272001263855088 2207 6 0.363595596958767 2207 7 0.28279494585441256 2207 8 0.27818783618172915 2207 9 0.4136925284585189 2207 10 0.5724429164145945 2207 11 0.5020335947967141 2207 12 0.4042904177834714 2207 13 0.45432183936087966 2207 14 0.5289032384469508 2207 15 0.48891715745913444 2207 16 0.5922603985204518 2207 17 0.567572436916322 2207 18 0.5664705490945039 2207 19 0.6356135502852212 2207 20 0.5548988454444554 2207 21 0.9401657604808236 2207 22 0.8360934043645969 2207 23 0.7991312960699446 2207 24 0.9683256260809685 2207 25 0.8899037110886638 2208 1 0.12693492208742896 2208 2 0.1558377100823769 2208 3 0.13830500514338695 2208 4 0.15191400488100726 2208 5 0.08383034966675496 2208 6 0.21954780705583943 2208 7 0.2612610570019953 2208 8 0.20280675206925736 2208 9 0.2538542414142455 2208 10 0.30789712715120743 2208 11 -0.044205379121855515 2208 12 -0.27239027340606015 2208 13 -0.08187746652111372 2208 14 -0.4509670100220404 2208 15 0.09781652196870283 2208 16 0.2087230307496668 2208 17 0.2246690372333049 2208 18 0.31975984298563276 2208 19 0.4169662223916504 2208 20 0.3759283472998334 2208 21 0.6464286270266726 2208 22 0.6093841353771126 2208 23 0.643262291814942 2208 24 0.694453112693175 2208 25 0.5015362984165463 2209 1 0.055024909155772406 2209 2 0.014397433093630951 2209 3 0.09493082971079403 2209 4 -0.5453203997599658 2209 5 -0.029281529143808882 2209 6 0.13397382915408684 2209 7 0.14601570021921617 2209 8 0.1982457744512871 2209 9 0.28203105231550296 2209 10 0.38193259467916363 2209 11 0.3559581575323918 2209 12 0.34399346799383324 2209 13 0.38895911997956034 2209 14 0.4106138256071077 2209 15 -0.22391193287165123 2209 16 -0.1556190925425156 2209 17 0.15509635833947755 2209 18 -0.22085892599637447 2209 19 0.23849456021534365 2209 20 0.2988382325585448 2209 21 0.6216178170621944 2209 22 0.5557288605799968 2209 23 0.5709065530875321 2209 24 0.7234391161086364 2209 25 0.6992259712178281 2210 1 -0.1432904321228179 2210 2 -0.18337302043477877 2210 3 -0.1164826324353676 2210 4 -0.03516736315398106 2210 5 -0.07573329939420859 2210 6 -0.5408911209881495 2210 7 -0.2306467626145121 2210 8 -0.33706001459600543 2210 9 -0.00841294344415644 2210 10 0.11987390361010597 2210 11 0.0757624771219696 2210 12 0.013379691403940797 2210 13 0.1288002727234101 2210 14 0.061015858920761445 2210 15 0.07093036687862053 2210 16 0.12523163917753521 2210 17 -0.9907722136685635 2210 18 -1.4275940983713014 2210 19 -0.36020251690025873 2210 20 -0.17337149720800937 2210 21 -0.373532876976185 2210 22 -0.35218264210349304 2210 23 -0.2383093848413509 2210 24 0.20494544836416395 2210 25 0.20768603907666972 2211 1 -0.2646377165980489 2211 2 -0.3148206911306133 2211 3 -0.29685750196915706 2211 4 -0.24786815590121591 2211 5 -0.21155243430337833 2211 6 -0.2285050039469212 2211 7 -0.33918957516146975 2211 8 -0.25530364154995056 2211 9 -0.22356678866079704 2211 10 -0.037647465097229826 2211 11 -0.47651107008344695 2211 12 -0.24652144194302394 2211 13 -0.24011299618116055 2211 14 -0.37715703199391726 2211 15 -0.405557878640181 2211 16 -0.4296264420113687 2211 17 -0.467941726337493 2211 18 -0.5501304698470193 2211 19 -0.3562551947911956 2211 20 -0.5020787513325047 2211 21 -0.06839931071017702 2211 22 -0.20939278712912568 2211 23 -0.886471283427646 2211 24 -0.2460670269809384 2211 25 -0.9470507812975839 2212 1 -0.16860531772659826 2212 2 -0.369171435606944 2212 3 -0.17184389347951878 2212 4 -0.012839862196166507 2212 5 -0.09961908997291147 2212 6 -0.15265268493156015 2212 7 -0.3047543955238865 2212 8 -0.43967782664968524 2212 9 -0.16284331721600864 2212 10 -0.5144724141297559 2212 11 -0.19846188109425356 2212 12 -0.36378660170852584 2212 13 -0.36739113090594344 2212 14 -0.3074546239165059 2212 15 -0.16985127528609212 2212 16 -0.2835417883786917 2212 17 -0.5370577510328545 2212 18 -0.5499064814635233 2212 19 -0.47232480736484916 2212 20 -0.6565241897483887 2212 21 -0.1126712215738408 2212 22 -0.47626301565244233 2212 23 -0.29821986032903425 2212 24 -0.6155437961857331 2212 25 -0.2574748199957142 2213 1 -0.20204330468717302 2213 2 -0.26567840574350754 2213 3 -0.1399752350628809 2213 4 -0.013249691510829109 2213 5 -0.08757187980287995 2213 6 -0.3371860575054271 2213 7 -0.49069742573507114 2213 8 -0.32800848964999574 2213 9 -0.3346670027587635 2213 10 -0.32767714686035276 2213 11 -0.06757288165834296 2213 12 -0.044517485949313595 2213 13 0.017496504628553566 2213 14 -0.5646785976868429 2213 15 -0.2314640392949059 2213 16 -0.36184913034860244 2213 17 -0.24300009307048498 2213 18 -0.2785722410588747 2213 19 -0.35360396094066715 2213 20 -0.3409890012016419 2213 21 -0.1219035822763459 2213 22 -0.4266918978572676 2213 23 -0.5915790221336437 2213 24 -0.7712934313256383 2213 25 -0.4512382105536119 2214 1 -0.3268846561247578 2214 2 -0.2331805624185641 2214 3 -0.20989404954155688 2214 4 -0.13392974334981034 2214 5 -0.2006171049833867 2214 6 -0.22474115612160972 2214 7 -0.2817579583845742 2214 8 -0.26801786478540635 2214 9 -0.385369153131208 2214 10 -0.19736355252009474 2214 11 -0.09826660228718254 2214 12 -0.39437154108929207 2214 13 -0.41403861470746506 2214 14 -0.4951812703882652 2214 15 -0.2937031210043344 2214 16 -0.5214990202498484 2214 17 -0.5794091010787454 2214 18 -0.6463272900419881 2214 19 -0.7782077826870406 2214 20 -0.6564197650388337 2214 21 -0.7792242631160136 2214 22 -0.9445641360329731 2214 23 -1.255182619349469 2214 24 -0.8793698347400749 2214 25 -0.9405485866225902 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 11.0 9 39.0 10 110.0 11 231.0 12 650.0 13 1527.0 14 3229.0 15 5762.0 16 8954.0 17 11536.0 18 13756.0 19 15334.0 20 16240.0 21 17360.0 22 18618.0 23 20681.0 24 22544.0 25 25664.0 26 29060.0 27 32470.0 28 38704.0 29 47683.0 30 60791.0 31 79554.0 32 106487.0 33 143064.0 34 195520.0 35 271415.0 36 387150.0 37 617947.0 38 1360366.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.4935089085567 21.581174426035144 12.826512775374088 24.09880389003407 2 14.377393236628489 22.52265261893241 39.93842011969737 23.161534024741734 3 17.797651516112527 28.141072533506982 29.917249136338075 24.144026814042416 4 11.59558687526411 18.442298385632373 38.64347286531884 31.318641873784674 5 12.529581379660062 38.71881267345845 34.810565138444375 13.941040808437108 6 29.53614177303004 39.701105180501926 17.46789953764523 13.294853508822808 7 24.833943871761587 34.597650013656164 23.452390673262922 17.116015441319327 8 23.867286146648492 37.332641434898996 21.331626675556603 17.46844574289591 9 24.647152462170528 17.024058010587044 22.48324046328525 35.84554906395718 10 14.868428601669347 30.576985525973292 33.08629163017283 21.468294242184527 11 31.363085815367107 26.81787965680613 23.037789633201697 18.78124489462506 12 21.122324775392045 28.349470725805453 31.87335815778125 18.654846341021255 13 26.497471045383815 24.391491407634604 27.441719193707115 21.669318353274466 14 20.44499368034658 23.580189223653804 29.373354989992713 26.601462106006906 15 22.97753476360635 30.01495880838482 25.5891051232089 21.418401304799936 16 20.64497895400282 31.495075098727444 26.99666737697318 20.863278570296558 17 19.114860254426798 30.1426674953891 29.761269386587756 20.98120286359634 18 19.450683485852444 29.26002357245744 31.277363150118042 20.011929791572072 19 19.92130463825778 28.53526020054425 32.062307719807805 19.48112744139017 20 21.50887203356552 27.572931958321778 31.116537718282146 19.801658289830552 21 21.58470306838856 28.45474856655306 30.276054476191227 19.684493888867152 22 20.392026819361444 28.151208816451266 31.190513120999313 20.266251243187977 23 20.090320586568676 28.534757774689464 31.102389135181653 20.27253250356021 24 20.28197932755874 27.955565426652356 31.128072132570704 20.634383113218195 25 20.75218401520999 27.73849919012544 30.471663961454816 21.037652833209755 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2436.0 1 2436.0 2 2733.0 3 3030.0 4 3030.0 5 3030.0 6 7041.5 7 11053.0 8 11053.0 9 11053.0 10 14411.0 11 17769.0 12 17769.0 13 17769.0 14 24624.0 15 31479.0 16 31479.0 17 31479.0 18 52979.0 19 74479.0 20 74479.0 21 74479.0 22 111564.0 23 148649.0 24 148649.0 25 148649.0 26 196931.5 27 245214.0 28 245214.0 29 245214.0 30 291738.0 31 338262.0 32 338262.0 33 338262.0 34 367743.5 35 397225.0 36 397225.0 37 397225.0 38 406620.0 39 416015.0 40 416015.0 41 416015.0 42 410374.5 43 404734.0 44 404734.0 45 404734.0 46 415457.5 47 426181.0 48 426181.0 49 426181.0 50 393580.5 51 360980.0 52 360980.0 53 360980.0 54 314521.0 55 268062.0 56 268062.0 57 268062.0 58 226196.0 59 184330.0 60 184330.0 61 184330.0 62 152325.5 63 120321.0 64 120321.0 65 120321.0 66 90104.5 67 59888.0 68 59888.0 69 59888.0 70 43426.0 71 26964.0 72 26964.0 73 26964.0 74 18735.5 75 10507.0 76 10507.0 77 10507.0 78 6987.5 79 3468.0 80 3468.0 81 3468.0 82 2244.5 83 1021.0 84 1021.0 85 1021.0 86 656.0 87 291.0 88 291.0 89 291.0 90 186.5 91 82.0 92 82.0 93 82.0 94 49.5 95 17.0 96 17.0 97 17.0 98 9.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09252745774124346 2 0.0 3 0.14254914694300483 4 0.011766497516227492 5 0.022970004720673766 6 0.01804383949258809 7 0.026713890294018876 8 0.024715274687195546 9 1.1259806235624394E-4 10 0.3994416262087754 11 0.06415274602747 12 0.0013230272326858663 13 8.444854676718296E-5 14 0.0013793262638639883 15 0.0010415320767952565 16 5.629903117812197E-5 17 8.444854676718296E-5 18 2.2519612471248789E-4 19 7.318874053155856E-4 20 1.1259806235624394E-4 21 2.2519612471248789E-4 22 0.0028149515589060985 23 5.629903117812197E-5 24 0.0 25 0.0016045223885764763 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3552459.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.1909260457138 #Duplication Level Percentage of deduplicated Percentage of total 1 86.20440075922141 62.23175520024027 2 10.272366669277888 14.831433250725906 3 1.9662244131684805 4.258306836009684 4 0.6408332388810536 1.850493798227895 5 0.2757218728649467 0.9952308666589533 6 0.15443500233526253 0.6689283499472743 7 0.09439613034512752 0.47701808453226624 8 0.06095264485637856 0.35201823016939854 9 0.04420733144195075 0.2872231375323802 >10 0.24530513555355254 3.212570699137587 >50 0.019997613361650533 1.0247770830496143 >100 0.017000914207640945 2.3806630389800034 >500 0.001961450236160802 1.0022370756634829 >1k 0.0018437632086810555 2.6374778984593314 >5k 1.1768701332006737E-4 0.5142305939980301 >10k+ 2.3537402664013473E-4 3.275635856667923 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 35686 1.0045436133112304 No Hit GGTATCAACGCAGAGTACTTTTTTT 21730 0.6116889737502952 No Hit TATCAACGCAGAGTACTTTTTTTTT 21308 0.5998098781717115 No Hit CCCATGTACTCTGCGTTGATACCAC 13216 0.37202399802503 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 12381 0.34851915250816407 No Hit GTACATGGGAAGCAGTGGTATCAAC 11345 0.3193562543578969 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 6559 0.184632672748651 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 6138 0.17278172668565633 No Hit GAGTACATGGGAAGCAGTGGTATCA 5461 0.15372450463186205 No Hit CATGTACTCTGCGTTGATACCACTG 4603 0.1295722202564477 No Hit ATCAACGCAGAGTACTTTTTTTTTT 4237 0.11926949755085141 No Hit GCGTTGATACCACTGCTTCCCATGT 4034 0.11355514588627202 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 3903 0.10986755934410504 No Hit GTATCAACGCAGAGTACATGGGAAG 3698 0.10409690864834752 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.8149515589060986E-5 0.0 3 0.0 0.0 0.0 2.8149515589060986E-5 0.0 4 0.0 0.0 0.0 5.629903117812197E-5 0.0 5 0.0 0.0 0.0 5.629903117812197E-5 0.0 6 0.0 0.0 0.0 5.629903117812197E-5 0.0 7 0.0 0.0 0.0 5.629903117812197E-5 0.0 8 0.0 0.0 0.0 5.629903117812197E-5 0.0 9 0.0 0.0 0.0 8.444854676718295E-5 0.0 10 0.0 0.0 0.0 1.1259806235624394E-4 0.0 11 0.0 0.0 0.0 1.1259806235624394E-4 0.0 12 0.0 0.0 0.0 1.688970935343659E-4 0.0 13 0.0 0.0 0.0 2.2519612471248789E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCGA 35 0.0021356966 16.325665 6 GACCGTG 75 1.4430356E-5 12.697739 7 CTGCTCG 55 0.0030161953 12.1193695 9 AGACCGT 65 7.864549E-4 11.720989 6 ACCGTTC 65 7.864549E-4 11.720989 8 GGAGCGT 60 0.0057719457 11.11052 6 TACCGTC 70 0.0014620274 10.883777 7 CCCCGGT 335 0.0 10.755723 19 TCGCGCG 330 0.0 10.676587 9 CGCGCCT 355 0.0 10.416719 12 TATCGCG 340 0.0 10.363595 7 ACACCGT 75 0.0025957022 10.158191 6 ATACCGT 85 6.450436E-4 10.083498 6 CAGTGCG 85 6.4563076E-4 10.0825 9 CCCCCGG 360 0.0 10.008515 18 CGCCTTT 400 0.0 9.955979 14 GCGCGCC 365 0.0 9.87753 11 CTAGGAC 320 0.0 9.793357 3 GCGCCTT 360 0.0 9.745271 13 ATCGCGC 375 0.0 9.650282 8 >>END_MODULE