FastQCFastQC Report
Wed 25 May 2016
SRR1294597_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294597_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605926
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93060.579478755559098No Hit
GGTATCAACGCAGAGTACTTTTTTT52680.32803504021978597No Hit
TATCAACGCAGAGTACTTTTTTTTT51710.3219949113470982No Hit
GTACATGGGAAGCAGTGGTATCAAC32690.20355856994656044No Hit
CCCATGTACTCTGCGTTGATACCAC32620.20312268435780978No Hit
ATTCCATTCCATTCCATTCCATTCC31070.1934709320354736No Hit
GAATGGAATGGAATGGAATGGAATG24390.1518749929946959No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22310.138922964071819No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA21800.1357472262109213No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17180.10697877735337744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCG307.736433E-418.9950475
ACAGGTC855.3370342E-511.1735568
ATCGCCG803.7766524E-410.68571219
GTCCTAG909.5247255E-510.5544481
GTCTATA1355.6943463E-810.5544471
GTATTAG1659.295036E-1010.3625491
GTCTAGA750.002649957310.132271
CTAAGGC951.6482288E-49.9973943
GTCCTAC800.0045194969.4990021
GTCCTAA900.00111462419.4990021
CTCTTAC1304.2427437E-69.4990021
GTCCAGA1401.0596141E-69.4990021
ACTGATC1401.061866E-69.4975238
AGTAGAC900.00111621369.4975233
TATTCCG1002.762188E-49.4975225
TCTTACA2054.2382453E-109.2658762
CTATTAG1651.066619E-79.2111541
GTACAAA3850.09.1289111
GGGTAAT1252.7565697E-59.1190431
GGTATCA25800.09.0940071
Produced by FastQC (version 0.11.5)