FastQCFastQC Report
Wed 25 May 2016
SRR1294597_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294597_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605926
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT250771.5615289870143456No Hit
GGTATCAACGCAGAGTACTTTTTTT159800.995064529747946No Hit
TATCAACGCAGAGTACTTTTTTTTT155980.971277630476124No Hit
ACGCAGAGTACTTTTTTTTTTTTTT89780.5590544022576382No Hit
GTACTTTTTTTTTTTTTTTTTTTTT50480.3143357788590508No Hit
GAGTACTTTTTTTTTTTTTTTTTTT42200.2627767406468293No Hit
CCCATGTACTCTGCGTTGATACCAC37930.2361877197330388No Hit
GCAGAGTACTTTTTTTTTTTTTTTT31510.19621078430762062No Hit
GTACATGGGAAGCAGTGGTATCAAC31160.1940313563638673No Hit
ATTCCATTCCATTCCATTCCATTCC30010.18687037883439211No Hit
ATCAACGCAGAGTACTTTTTTTTTT29900.18618541576635536No Hit
GAATGGAATGGAATGGAATGGAATG26650.1659478705743602No Hit
GTGGTATCAACGCAGAGTACTTTTT18920.11781364770232251No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA16360.1018726890280125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGAC250.005954602719.0426126
GCACCGG350.002138002516.3222396
AGGACCG602.5146675E-514.281965
AAATCGC707.4027685E-613.54638717
GGTATCA38400.011.7836651
CCTACAC907.4771033E-611.6088433
AATCGCC907.6158376E-611.58968518
GCCTTAA1001.91455E-611.407381
TACACTA951.3267958E-511.0246715
TTAGACC803.6935447E-410.711474
TCGCGTT803.6956798E-410.710810
CGAGTAC1252.1733467E-710.66319710
TATAACG909.53345E-510.55349353
CCTAGAC1002.4036772E-510.447963
CTACACT1105.884973E-610.3868794
CCGGGGG1106.179129E-610.34451316
ATTACAC1606.188202E-910.0917793
ACGGTTC856.458762E-410.0813837
TAGGACC951.6071458E-410.0224284
TACACTG1951.5643309E-109.7654425
Produced by FastQC (version 0.11.5)