FastQCFastQC Report
Wed 25 May 2016
SRR1294596_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294596_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544212
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63101.1594746165097425No Hit
GGTATCAACGCAGAGTACTTTTTTT40080.7364776961919252No Hit
TATCAACGCAGAGTACTTTTTTTTT36260.6662844626726349No Hit
GTACTGGTTCACTATCGGTCAGTCA28980.5325130647615268No Hit
ATACAGGGTGACAGCCCCGTACACA19880.3652988173726415No Hit
GGATACCACGTGTCCCGCCCTACTC17070.3136645277943155No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16120.29620809537459664No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA13830.25412890564706403No Hit
ACCCTGTATCGCGCGCCTTTCCAGA13500.24806509228021434No Hit
GTTAATGATAGTGTGTCGAAACACA12050.22142106385011723No Hit
GGTATCAACGCAGAGTACATGGGGG11480.21094720439828596No Hit
GTTCACTATCGGTCAGTCAGGAGTA10550.19385827581898232No Hit
GTATTTAGCCTTGGAGGATGGTCCC9540.17529933187801813No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9280.1705217819526214No Hit
ACTTAGATGTTTCAGTTCCCCCGGT8830.16225294554328093No Hit
GTATCAACGCAGAGTACATGGGGGT8580.1576591475380918No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG8370.1538003572137329No Hit
GAGTACTTTTTTTTTTTTTTTTTTT8190.1504928226499967No Hit
CCCATATTCAGACAGGATACCACGT8120.14920655920854373No Hit
GCCCAGAGCCTGAATCAGTGTGTGT8040.1477365438468832No Hit
GTCAGGAGTATTTAGCCTTGGAGGA7970.14645028040543023No Hit
AGCGTACACGGTGGATGCCCTGGCA7540.1385489478365049No Hit
GCCTTGGAGGATGGTCCCCCCATAT7540.1385489478365049No Hit
GGTTAATGAGGCGAACCGGGGGAAC7430.13652767671422167No Hit
CTATCGGTCAGTCAGGAGTATTTAG7280.13377139791110818No Hit
TCTAAGTACCCCGAGGAAAAGAAAT7130.13101511910799468No Hit
TATTCAGACAGGATACCACGTGTCC7090.13028011142716442No Hit
ATCATTAACTGAATCCATAGGTTAA6910.12697257686342822No Hit
GGTATCAACGCAGAGTACATGGGGA6870.12623756918259796No Hit
GTACATGGGGGTTAAGCGACTAAGC6760.12421629806031473No Hit
CGGTACTGGTTCACTATCGGTCAGT6730.12366504229969204No Hit
GTGGATGCCCTGGCAGTCAGAGGCG6690.12293003461886177No Hit
GTGGTATCAACGCAGAGTACATGGG6690.12293003461886177No Hit
GTACTTAGATGTTTCAGTTCCCCCG6660.12237877885823906No Hit
TCACGGTACTGGTTCACTATCGGTC6510.11962250005512559No Hit
TCATTAACCTATGGATTCAGTTAAT6460.11870374045408776No Hit
GGGTTTCCCCATTCGGAAATCGCCG6380.11723372509242722No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6230.11447744628931374No Hit
CACACACACTGATTCAGGCTCTGGG6170.11337493476806831No Hit
ATCAACGCAGAGTACTTTTTTTTTT6020.11061865596495483No Hit
GGTTCACTATCGGTCAGTCAGGAGT5930.10896488868308674No Hit
CATCTAAGTACCCCGAGGAAAAGAA5780.10620860987997326No Hit
TTCCAGACGCTTCCACTAACACACA5710.10492234643852028No Hit
AGCTCACAGCATGTGCATTTTTGTG5700.10473859451831272No Hit
GTCCCGCCCTACTCATCGAGCTCAC5570.10234981955561437No Hit
ACGCAGAGTACATGGGGGTTAAGCG5520.10143105995457653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12800.015.5848811
GTATGGA400.005275632714.2490341
AATCGCC1009.973519E-913.30399518
ATCGCCG1001.002627E-813.29909919
CGAGAGG853.9168226E-612.29781116
AAATCGC1103.7771315E-812.0945417
ACGCTTC1201.2710916E-711.0815627
AGAGATC600.00587431511.0815628
GTACTGG6150.010.8122751
TACTGGT6100.010.74418452
AGCGTAC1951.8189894E-1210.7172211
GAAATCG1252.2184759E-710.64319616
ACTGGTT6150.010.501423
ACGGTGG1856.002665E-1110.2686298
TCCTAGT750.002636257610.13637616
CCGAGAG750.002636257610.13637615
GTATATA750.00264432310.1326461
GAATAAG856.5823604E-410.0581411
AGGGTGA2850.09.9984025
ACAGGGT2950.09.9805373
Produced by FastQC (version 0.11.5)