##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294596_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	544212
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.042213328629284	31.0	28.0	33.0	20.0	34.0
2	29.029964425628247	31.0	28.0	33.0	18.0	34.0
3	29.067080475990974	31.0	28.0	34.0	18.0	34.0
4	32.243752434712945	35.0	32.0	37.0	18.0	37.0
5	33.085830521928955	35.0	33.0	37.0	26.0	37.0
6	33.054032252137034	35.0	33.0	37.0	25.0	37.0
7	32.94588505949887	35.0	33.0	37.0	23.0	37.0
8	32.73409259626763	35.0	32.0	37.0	22.0	37.0
9	33.43686651525508	38.0	32.0	39.0	17.0	39.0
10	33.71784341396368	38.0	32.0	39.0	17.0	39.0
11	33.93267880899355	38.0	33.0	39.0	18.0	39.0
12	33.799576268072	38.0	33.0	39.0	18.0	39.0
13	33.551954017919485	37.0	32.0	39.0	17.0	39.0
14	34.292031414228276	38.0	32.0	41.0	17.0	41.0
15	34.31280273128854	38.0	32.0	41.0	17.0	41.0
16	34.50432184516328	38.0	32.0	41.0	17.0	41.0
17	34.59028099343638	38.0	33.0	41.0	18.0	41.0
18	34.515220171550794	38.0	32.0	40.0	18.0	41.0
19	34.868163509808674	39.0	33.0	41.0	21.0	41.0
20	34.982683219039636	39.0	33.0	41.0	22.0	41.0
21	35.06919362307336	39.0	33.0	41.0	23.0	41.0
22	35.15533101070906	39.0	33.0	41.0	24.0	41.0
23	35.20058359609858	39.0	33.0	41.0	24.0	41.0
24	35.2750508992819	39.0	33.0	41.0	25.0	41.0
25	34.21226654318538	38.0	31.0	40.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.3056344468860566
1101	2	1.2189486119286705
1101	3	1.4615391090023593
1101	4	1.250291224303588
1101	5	0.9784970641428004
1101	6	0.9510330867536041
1101	7	0.6575788766225372
1101	8	1.1230592200878888
1101	9	1.2109825147714517
1101	10	1.2829376968494302
1101	11	1.0223818909453826
1101	12	1.2648834819804406
1101	13	1.382450488354074
1101	14	1.2553820661138744
1101	15	1.6479986883399036
1101	16	1.4205253386731087
1101	17	1.293985552190172
1101	18	1.2957925236554146
1101	19	1.3789302420725278
1101	20	1.1486297930115157
1101	21	1.0612770800695515
1101	22	1.153028164467095
1101	23	1.24639470035941
1101	24	1.2866999538760737
1101	25	1.189632517026098
1102	1	1.2607424856309883
1102	2	1.4762527987593081
1102	3	1.5836274064983904
1102	4	1.4737048873852672
1102	5	1.3649096208716145
1102	6	0.9159515402679688
1102	7	1.0540295159740722
1102	8	1.0084896064082471
1102	9	1.4773200351862954
1102	10	1.3257844105298275
1102	11	1.0592565569698138
1102	12	1.2875674790000602
1102	13	1.5215969408749785
1102	14	1.6570314350411621
1102	15	1.9082079922407331
1102	16	1.489926402879206
1102	17	1.5615253846567327
1102	18	1.6185879085834287
1102	19	1.3998135933433318
1102	20	0.6056852386007776
1102	21	0.6789230320974866
1102	22	1.0003627571862452
1102	23	1.0204936248640024
1102	24	1.0928473622256476
1102	25	1.0336461695710923
1103	1	0.8064171290141289
1103	2	0.740973249563698
1103	3	1.065041330658687
1103	4	0.8557557686159427
1103	5	0.5351656843940447
1103	6	0.4495707069879984
1103	7	0.5448895021877789
1103	8	0.30686406635076935
1103	9	0.510723555588882
1103	10	0.4223449930197205
1103	11	0.4570232521382067
1103	12	0.9512854098501009
1103	13	0.6214054017441129
1103	14	0.4862461434383718
1103	15	0.9199404702711789
1103	16	0.9112230305098095
1103	17	0.616191255284015
1103	18	1.0197088927593185
1103	19	0.7034652670977621
1103	20	-0.2549111882621631
1103	21	0.22599796401351568
1103	22	-0.10801411347493683
1103	23	0.4665905206870562
1103	24	0.5457705469356711
1103	25	0.49402845681574803
1104	1	0.47988242927783986
1104	2	0.4071531073598713
1104	3	0.6902958621538318
1104	4	0.5985203979179445
1104	5	0.4637587047602736
1104	6	0.31895107811253354
1104	7	0.2907984531656638
1104	8	0.4288982412607325
1104	9	0.9903647449974073
1104	10	0.4696451973867184
1104	11	0.4209975412118041
1104	12	0.6963663221206531
1104	13	0.5803391326843865
1104	14	0.882943446609957
1104	15	0.8202605586942155
1104	16	0.6188901311318133
1104	17	0.28691188077313967
1104	18	0.51508745388211
1104	19	-0.12245558983421034
1104	20	0.3579960157673199
1104	21	-0.4938634670005655
1104	22	-0.023632054746315134
1104	23	-0.4802555141851528
1104	24	-0.6042954736197217
1104	25	-2.1852813316588424
1105	1	-0.08205507704231252
1105	2	-0.042277236119417694
1105	3	0.7690618310755291
1105	4	0.4427581031122685
1105	5	0.2291574081456318
1105	6	0.18552280202575133
1105	7	0.018781846610679054
1105	8	0.2018221409921921
1105	9	0.6195430688364638
1105	10	-1.4471843887977016
1105	11	-0.5574521391288627
1105	12	0.056266772677389554
1105	13	0.19891645204035058
1105	14	0.7903854978228608
1105	15	-0.20236840053096472
1105	16	0.21016157752005427
1105	17	0.20036229144267992
1105	18	-0.30844823672810406
1105	19	-1.430755496576829
1105	20	-0.22032052000194113
1105	21	-1.3984263370806786
1105	22	-0.7944042932604845
1105	23	-0.288223963977849
1105	24	-0.5824895388549436
1105	25	-0.5964368242937539
1106	1	-0.7324290812700482
1106	2	-0.6508226757409439
1106	3	-0.04785891541921572
1106	4	-0.05826898274614223
1106	5	-0.21176889857727588
1106	6	-0.5650461726259621
1106	7	-0.4337520760045024
1106	8	-0.12466430861663724
1106	9	-0.272112431536101
1106	10	-0.6953554084535796
1106	11	-0.7887527272381192
1106	12	-0.5091367969123368
1106	13	-0.48196631946049706
1106	14	-0.8586165712727762
1106	15	-0.8152993421986707
1106	16	-0.7875294780385218
1106	17	-0.05050356272285228
1106	18	-0.35169244224761087
1106	19	-0.6411050255670858
1106	20	-0.7241799536816131
1106	21	-0.3865628824691285
1106	22	-0.9100369984031644
1106	23	-1.0741210677769502
1106	24	-0.42264470503818075
1106	25	-0.5578073096731018
1107	1	0.38988146875531626
1107	2	0.5357430602942657
1107	3	0.5637069978116287
1107	4	0.33745037582592374
1107	5	0.4430621124807459
1107	6	0.4621356371793368
1107	7	0.40182151637871755
1107	8	0.25729555606665144
1107	9	0.6685523457188651
1107	10	-1.3970369443650625
1107	11	-0.28433549329229635
1107	12	0.11884148194093314
1107	13	0.355395429976781
1107	14	0.2853250088464776
1107	15	0.5464369319746112
1107	16	0.3701067929958626
1107	17	-0.04404149119454104
1107	18	-0.22662270310996036
1107	19	0.08875264077657619
1107	20	0.27387291946960346
1107	21	0.42085280531824054
1107	22	0.22567156642360686
1107	23	0.4322088664379038
1107	24	0.45693516918329635
1107	25	0.29923024263757725
1108	1	-0.3519941115603764
1108	2	0.07706153754589096
1108	3	0.4764275177744892
1108	4	-0.15558583587323938
1108	5	-0.017476420481187915
1108	6	0.06102143105120206
1108	7	0.09727184135550004
1108	8	0.24986751521242923
1108	9	0.5264910643818226
1108	10	0.6020345505281597
1108	11	0.24305540735765874
1108	12	-0.19406473904328436
1108	13	-0.0011505310260062629
1108	14	0.41438721868863126
1108	15	0.031116597712774308
1108	16	0.3608217419280848
1108	17	0.05623706033748732
1108	18	0.014788624652346982
1108	19	-0.08023528865703611
1108	20	-0.012106653418051394
1108	21	0.2416513197100727
1108	22	0.0678256982713279
1108	23	0.06730635947411656
1108	24	0.28423321932257295
1108	25	-0.10095545838377973
1109	1	-0.2032404901367677
1109	2	-0.3412452344528276
1109	3	0.00822501233284001
1109	4	-0.11990181174225256
1109	5	0.37332782730283043
1109	6	0.008119472057636301
1109	7	-0.26307693154635103
1109	8	-0.08887702411204401
1109	9	0.08941631211002488
1109	10	0.13949947789674155
1109	11	-0.07052309191425365
1109	12	-0.21635900920275475
1109	13	-0.3495961728868693
1109	14	0.05421095493229444
1109	15	-0.791541463791809
1109	16	-0.1851265763614336
1109	17	0.19502066790962402
1109	18	0.1012943504338395
1109	19	0.07505084820360253
1109	20	0.02741062540239625
1109	21	0.0503234559314123
1109	22	-0.2977668452745448
1109	23	-0.23410263434092116
1109	24	0.11941848907106589
1109	25	0.028225985720723656
1110	1	-0.11702253287522879
1110	2	0.017254032507054262
1110	3	0.18520755265004496
1110	4	-0.1272659771871858
1110	5	0.08420258850945572
1110	6	0.1275250431625281
1110	7	-0.18210221032373397
1110	8	0.06631156711812736
1110	9	0.3248288137760298
1110	10	0.29373733830812654
1110	11	-0.24409544075249556
1110	12	0.374618821887033
1110	13	0.030805444244059288
1110	14	0.05474140941716854
1110	15	0.05506478259852088
1110	16	-0.20482447497976608
1110	17	0.0457381586910941
1110	18	-4.567377736287032
1110	19	-1.6364823163083742
1110	20	-1.0091463073800497
1110	21	-0.4524692047820835
1110	22	-0.3463962823885893
1110	23	-0.287107210288319
1110	24	-0.01029584741656464
1110	25	-0.10015377348670995
1111	1	-0.27148786143193604
1111	2	-0.524453775315493
1111	3	-1.3606824213402362
1111	4	-0.6955890865752252
1111	5	-0.43644520066460757
1111	6	-0.6570408890159101
1111	7	-0.816591094850402
1111	8	-2.202075339688566
1111	9	-0.5693999735822715
1111	10	-0.4511573959510855
1111	11	-0.4400114244596196
1111	12	-0.11929392287422047
1111	13	-0.7758824882288309
1111	14	-0.49380940768053705
1111	15	-0.26584997423515944
1111	16	-2.6281380147550024
1111	17	-1.7748880375105074
1111	18	-0.7251085111802951
1111	19	-0.3486479932971349
1111	20	-0.49051844263057376
1111	21	-0.44650267388940534
1111	22	-0.4438198481735398
1111	23	-0.39596818099057884
1111	24	-0.08171362235889745
1111	25	-0.4203873701888696
1112	1	-0.20685108680930497
1112	2	-0.16743930354764558
1112	3	0.18077342671882946
1112	4	-0.05546171144578693
1112	5	-1.2693926763134833
1112	6	-0.21196878273807584
1112	7	-0.3328328971285046
1112	8	0.1710630723737694
1112	9	-0.05435783792312776
1112	10	-0.027100502287851214
1112	11	-0.22051242207712107
1112	12	-1.2255465011051072
1112	13	-0.8837072611340417
1112	14	-2.5085905632331027
1112	15	-3.5513348500170494
1112	16	-0.7756296591822363
1112	17	-0.1700287563002334
1112	18	0.31131294643569873
1112	19	0.20433307301706805
1112	20	0.06399482947065138
1112	21	0.24770020025925277
1112	22	0.6266468852815805
1112	23	0.3677943102750021
1112	24	0.09669364130677849
1112	25	0.16703675615917746
1113	1	-0.6086962972319192
1113	2	0.02569899314713453
1113	3	-0.3874727932010309
1113	4	-2.0914084471833156
1113	5	-0.42262151782684043
1113	6	-0.638857715903157
1113	7	-0.5559904539680716
1113	8	-1.0806803028005696
1113	9	-2.6331528612788198
1113	10	-0.6136881359466102
1113	11	-0.6923029513990286
1113	12	-2.5158212348944886
1113	13	-1.7833718689576479
1113	14	-0.9547230376082538
1113	15	-0.40807368688140855
1113	16	-0.771777630225607
1113	17	-0.8143470135892059
1113	18	-0.2823035331640469
1113	19	-0.04299928623997573
1113	20	0.056727998982061933
1113	21	-0.0371949758493173
1113	22	-0.3384468695611247
1113	23	-0.23176071396621722
1113	24	0.17735933389715797
1113	25	0.44703941273086656
1201	1	1.1915415179240867
1201	2	1.0935807068421326
1201	3	1.3045718569764375
1201	4	1.137466997765273
1201	5	0.8705149432595718
1201	6	0.6151843778886956
1201	7	0.37465358206576127
1201	8	0.6847993250467184
1201	9	1.1444161280322263
1201	10	0.9614375190635442
1201	11	0.690406308114845
1201	12	0.9874379021471285
1201	13	0.9751337148607391
1201	14	1.357968245727882
1201	15	1.0997991660347566
1201	16	1.0341407589459095
1201	17	0.9434634574070202
1201	18	1.242830015068634
1201	19	1.1188499684870479
1201	20	1.079610705695373
1201	21	0.7563845241786922
1201	22	0.8664866491356236
1201	23	0.9073076820800452
1201	24	0.761098786260284
1201	25	0.9507441349148991
1202	1	1.0662880737178675
1202	2	1.3834441405176428
1202	3	1.3213618133280995
1202	4	1.4855322234172945
1202	5	1.1780448133870394
1202	6	0.9357347852023139
1202	7	1.1992281240170755
1202	8	1.2449681121850205
1202	9	1.2643663128974154
1202	10	0.9649221746521732
1202	11	1.0695560669441377
1202	12	1.0829696102940503
1202	13	1.2192583858339887
1202	14	1.6008401418742366
1202	15	1.5842032751361899
1202	16	1.401087289037008
1202	17	1.3671176952530288
1202	18	1.3825943824543288
1202	19	1.4435673545053405
1202	20	1.1832165707155866
1202	21	0.8383215452003441
1202	22	0.7847252939568605
1202	23	0.8350248859605998
1202	24	1.129793653766633
1202	25	0.9580407392941552
1203	1	0.5906352589698649
1203	2	0.7403986983048547
1203	3	1.0275227639700972
1203	4	1.0096154229245258
1203	5	0.6974459323932791
1203	6	0.4416332166870163
1203	7	0.6057790036029118
1203	8	0.6896759312961223
1203	9	1.0708537625751333
1203	10	0.9822618216445633
1203	11	0.8088481451719147
1203	12	0.7698980994889695
1203	13	1.085918086210171
1203	14	1.272790189904974
1203	15	1.3905348998951155
1203	16	1.2158783736517762
1203	17	1.1654179203742032
1203	18	0.9911374377960058
1203	19	0.7974173299399965
1203	20	0.29272299070224506
1203	21	0.6202528209114604
1203	22	0.5835562404190782
1203	23	0.6820130652655791
1203	24	0.8728841935441523
1203	25	0.9804969433258037
1204	1	0.1413545778189551
1204	2	0.12615178109992442
1204	3	0.27932106109282273
1204	4	0.4006092573343878
1204	5	0.010966190760868244
1204	6	-0.1315978461432863
1204	7	0.10416420618658861
1204	8	0.18534533822506916
1204	9	-0.5936280342539888
1204	10	-0.5815300162884967
1204	11	-0.23329806739414494
1204	12	0.11746122338796994
1204	13	0.1545249564391753
1204	14	-1.4589405793257981
1204	15	0.07829179948407727
1204	16	-0.01922879017676138
1204	17	-0.17046728220947216
1204	18	0.20941400855349457
1204	19	0.4179032371625482
1204	20	-0.8800750128209529
1204	21	-0.9550114186657552
1204	22	-1.867221798336054
1204	23	-1.6658876980932007
1204	24	-0.5851457536079323
1204	25	-3.2994502087587527
1205	1	-0.5135974389751681
1205	2	-0.3755819870812118
1205	3	0.07590788070595877
1205	4	0.13777562410601973
1205	5	0.12792150640039068
1205	6	0.09325033199436206
1205	7	-0.022016630520568015
1205	8	0.11912476542555339
1205	9	0.4313138610826428
1205	10	0.34830016814695597
1205	11	0.15481484308718763
1205	12	0.22338789905148815
1205	13	0.5040132605327443
1205	14	0.5447200144663782
1205	15	-0.2542168224982291
1205	16	-0.5325684962796302
1205	17	-0.9645837164574829
1205	18	-0.8041637392347809
1205	19	-0.6172125972953921
1205	20	-0.043244153621671444
1205	21	-0.2445188410679151
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2111	18	-1.0877710804389409
2111	19	-0.906465604026053
2111	20	-0.812801695274544
2111	21	-0.4280673349441173
2111	22	-0.7305171921180431
2111	23	-0.4432958410922083
2111	24	-0.6927062913819739
2111	25	-0.4360803363273078
2112	1	-1.0872383313295089
2112	2	-0.06542387575350972
2112	3	-0.03547396897998567
2112	4	0.2951288837984549
2112	5	-0.7772658446933889
2112	6	-0.6875055701065662
2112	7	-0.19047017178112213
2112	8	0.20635673423646494
2112	9	-2.424599069391693
2112	10	-0.7052519219548472
2112	11	-0.7502100648616974
2112	12	-0.399419118492375
2112	13	0.11918545026633609
2112	14	0.01970377015188518
2112	15	-0.48596904255454376
2112	16	-0.8837705755518854
2112	17	-0.873723850743815
2112	18	-0.2731912462739814
2112	19	0.14968148977413165
2112	20	0.3552288808162771
2112	21	0.31201137335703066
2112	22	0.14714983825780337
2112	23	0.2789682689849897
2112	24	0.2676003685826345
2112	25	0.452330418021873
2113	1	0.30367142763580546
2113	2	-0.03696635076820698
2113	3	0.3532679475397096
2113	4	0.13875028297541547
2113	5	0.10201472555939972
2113	6	-0.46690004394548623
2113	7	0.2026074296298077
2113	8	0.14104588767561665
2113	9	-0.6477031258290822
2113	10	0.17847456246608573
2113	11	0.01404625495017342
2113	12	-0.06360562642888112
2113	13	0.23547733739155774
2113	14	0.39332590419068936
2113	15	-2.0773858561935796
2113	16	-0.3035236619716386
2113	17	-1.6516486008907876
2113	18	0.35179038217987113
2113	19	0.3678803433896505
2113	20	0.5258086868127521
2113	21	0.3465682516639106
2113	22	0.6281536595394002
2113	23	0.9537551590496562
2113	24	0.6176701804579992
2113	25	1.0477338571753165
2114	1	-0.550355675585319
2114	2	-0.6163238704466529
2114	3	-3.0113555102842184
2114	4	-0.9976311160085665
2114	5	-0.853857057503177
2114	6	-1.6608374078711243
2114	7	-3.2074509078904256
2114	8	-1.1576634993617283
2114	9	-0.6571515710922995
2114	10	-0.6908015917236128
2114	11	-0.46624738653924425
2114	12	-0.2806878432546469
2114	13	-3.2059234609113503
2114	14	-2.5964946870000674
2114	15	-1.3968186703508962
2114	16	-1.642523271193987
2114	17	-1.6629113709460555
2114	18	-0.6153267255072876
2114	19	-0.5351527191905916
2114	20	0.0803204240985167
2114	21	-0.08953436933759917
2114	22	-0.19967738495342502
2114	23	0.12408885524794755
2114	24	-0.26522736601662444
2114	25	-0.3486769199139488
2201	1	1.264114099850321
2201	2	1.124779488344135
2201	3	1.4447150118023089
2201	4	0.990853842845965
2201	5	0.6320453543157569
2201	6	0.6471082778298296
2201	7	0.7559939298609706
2201	8	1.0312938072317905
2201	9	1.4182931756041128
2201	10	1.0985017238160637
2201	11	0.8232926719635856
2201	12	1.140818206210973
2201	13	1.1391123350799575
2201	14	1.585411549128274
2201	15	1.3467010601263496
2201	16	1.381265982041306
2201	17	1.3642321203297385
2201	18	1.4697948898081634
2201	19	1.3342493910087043
2201	20	1.2025799535718775
2201	21	1.3308175581826376
2201	22	1.0339731232472147
2201	23	1.1190637582174787
2201	24	1.262893828169517
2201	25	1.1495975881045766
2202	1	0.9855964310665719
2202	2	0.8627909224580854
2202	3	1.0629564338852546
2202	4	0.9629577757708034
2202	5	0.7880352072391048
2202	6	0.7176752270851168
2202	7	0.4518698146991085
2202	8	0.9404532226557194
2202	9	1.2000051217350745
2202	10	1.0638654983793572
2202	11	0.7852140880776659
2202	12	0.9763413682191242
2202	13	0.7110931600895114
2202	14	-1.0274019330537172
2202	15	0.6841072136568371
2202	16	0.8544974715538203
2202	17	1.085657560766954
2202	18	0.9868210875455858
2202	19	0.798994828088432
2202	20	0.7490186840681332
2202	21	0.6615684232022687
2202	22	0.5512958991441792
2202	23	0.8609614853842302
2202	24	1.1931942301355107
2202	25	1.01183516772835
2203	1	0.967026721209546
2203	2	0.7713814300917079
2203	3	1.2680159826760935
2203	4	0.990853842845965
2203	5	0.8951521504322599
2203	6	0.9121568215191473
2203	7	0.7977415026765016
2203	8	0.9613908946104317
2203	9	1.3377106513322659
2203	10	1.2538415296413064
2203	11	0.9776616040024209
2203	12	1.1971288858226217
2203	13	1.2556171894488841
2203	14	0.8329843646622521
2203	15	1.144759312553532
2203	16	1.2288387975752926
2203	17	1.417630178582165
2203	18	1.3833871228178722
2203	19	1.1109484201349176
2203	20	0.16568674968838337
2203	21	0.9434389174059348
2203	22	-0.1291336728692869
2203	23	0.08217055433398457
2203	24	0.41240839127630835
2203	25	0.9360053550948706
2204	1	0.7452370161353556
2204	2	0.46183755758758593
2204	3	0.6890271568838244
2204	4	0.6431527986986865
2204	5	0.25962366536173676
2204	6	0.5852562902863241
2204	7	0.3289102148948615
2204	8	0.7017096343063471
2204	9	1.1699967843236578
2204	10	-1.061591699887913
2204	11	-0.04610648539124185
2204	12	0.223956143562134
2204	13	0.7316475979486086
2204	14	0.9699600012257292
2204	15	0.6934587080472099
2204	16	0.7099983506069805
2204	17	0.6753055770067817
2204	18	0.7826360695114047
2204	19	0.6851927872351169
2204	20	-0.3303290041126772
2204	21	0.20932094708170013
2204	22	0.4540409012285309
2204	23	0.35486701888793704
2204	24	0.5111169459566511
2204	25	-0.06458083285163951
2205	1	0.3670943679601173
2205	2	0.32765495162909986
2205	3	0.53147720965476
2205	4	0.42857691038791046
2205	5	0.20968476855206575
2205	6	0.1343897359507693
2205	7	0.41997155879472814
2205	8	0.4675696154199045
2205	9	0.854247899919784
2205	10	0.6981644428343259
2205	11	0.45394998876951576
2205	12	0.3922611780946994
2205	13	0.5341741222880501
2205	14	0.7776969612494327
2205	15	0.15019487187782943
2205	16	-0.10807060341749519
2205	17	0.4191841483030956
2205	18	-0.10746151043193919
2205	19	-0.7558389308754982
2205	20	0.20960378237796107
2205	21	-0.8294490271808925
2205	22	-1.6584047076740163
2205	23	-1.932276314383678
2205	24	-1.041760458824207
2205	25	-2.0040720099212805
2206	1	-0.10353990822597581
2206	2	-0.4639179942574607
2206	3	0.3545848459805008
2206	4	0.16161163990756933
2206	5	0.11901588443002709
2206	6	-0.0693020667014892
2206	7	-0.0161557393545948
2206	8	-0.006196526989363349
2206	9	0.10061124416669998
2206	10	-0.32546296387188534
2206	11	0.21263453982164293
2206	12	0.3289486297868791
2206	13	0.42255592989050683
2206	14	0.03163974742709996
2206	15	-0.047729303306937254
2206	16	-0.7600133374878624
2206	17	-0.24140839861519225
2206	18	0.002351380915648349
2206	19	-0.7661930868674034
2206	20	-0.41167814420284543
2206	21	-2.7237146181211713
2206	22	-1.4148562435368746
2206	23	-2.3475248662347923
2206	24	-1.194948340401666
2206	25	-2.0544706308145706
2207	1	0.5742613550565849
2207	2	0.48515592751113346
2207	3	0.8392831714731486
2207	4	0.41043553700267665
2207	5	0.3255000769490266
2207	6	0.22561190507204287
2207	7	0.10380675694437969
2207	8	0.45246715692681505
2207	9	-0.9716985232445552
2207	10	-0.21364683070084567
2207	11	0.07410113191954792
2207	12	0.2674540744031262
2207	13	0.5448130605283978
2207	14	0.6352437215034854
2207	15	0.5425468752633194
2207	16	0.8197724966405104
2207	17	0.5363492748394236
2207	18	0.4623021984305353
2207	19	-0.7142636201437114
2207	20	-0.13015365165423276
2207	21	-0.31286815300948234
2207	22	0.28367897375430573
2207	23	-0.19544160143892242
2207	24	-1.7086873606997273
2207	25	-0.042265478990380245
2208	1	0.2757532044403028
2208	2	-0.24079782700162156
2208	3	0.26466388553618714
2208	4	-0.17654878525422646
2208	5	-0.1476538411459103
2208	6	-0.14112392646512717
2208	7	-0.10111428448414728
2208	8	0.09485321087541365
2208	9	-0.8804846965276951
2208	10	-0.5047812127926221
2208	11	-0.22544449175427417
2208	12	-0.10931792622707093
2208	13	0.09748032163304998
2208	14	0.38144663715474536
2208	15	0.06126329147269871
2208	16	-0.10048789401251668
2208	17	-0.016101776209609397
2208	18	0.285494330395764
2208	19	-1.7097542158443204
2208	20	-0.6490820878018724
2208	21	-0.5976503171883394
2208	22	-0.26514092159332137
2208	23	0.09790851471112916
2208	24	0.14558706181830416
2208	25	0.09939317058508124
2209	1	0.31262698175228465
2209	2	-1.3298728719442217
2209	3	-0.18472367703396486
2209	4	0.4101313648732656
2209	5	0.16646692302950328
2209	6	0.1475281389323797
2209	7	-0.16095191069743464
2209	8	0.23089419100420372
2209	9	-1.9346586151871108
2209	10	-0.055714931554554425
2209	11	-0.13168184590031728
2209	12	0.21441184755980913
2209	13	0.36795424021070744
2209	14	0.6014393286175732
2209	15	0.7484570829766497
2209	16	0.7655304329210821
2209	17	0.36211256994200625
2209	18	0.7703282982529132
2209	19	0.3327988017068009
2209	20	0.5857925511653832
2209	21	0.42953596957610074
2209	22	0.5975525931835719
2209	23	0.5728702348170458
2209	24	0.4600683477938361
2209	25	0.6162604405194401
2210	1	0.08836138763064127
2210	2	0.00543831434987041
2210	3	-0.12608422693441668
2210	4	-0.06359840717815501
2210	5	-0.20472243407409962
2210	6	-0.05549212034243567
2210	7	-0.20981675413583645
2210	8	-0.031899814995689724
2210	9	-0.06740244186986644
2210	10	-1.5631009634082247
2210	11	-1.3252878526766168
2210	12	-2.331216769802218
2210	13	-0.983720301013399
2210	14	-0.5542197468610581
2210	15	-0.5307346753960545
2210	16	-0.10343332773496172
2210	17	-0.07386437066753615
2210	18	0.1821434922585894
2210	19	0.33840476589578117
2210	20	0.006463448717511255
2210	21	0.11327605267049279
2210	22	0.057217087078441864
2210	23	-0.13097351561803805
2210	24	-0.1868812132702189
2210	25	-0.2179496181118168
2211	1	0.02602554269223134
2211	2	0.21990641278808098
2211	3	-1.196140255047684
2211	4	-0.2726177324388459
2211	5	-0.4002647266839219
2211	6	-0.20403189090392004
2211	7	0.0319839779369957
2211	8	0.3249073657928747
2211	9	0.5168303176060931
2211	10	0.4592084600713733
2211	11	0.1293779367558443
2211	12	0.21939912171315257
2211	13	0.2549343976723222
2211	14	-0.077884183890788
2211	15	0.28223429244390985
2211	16	0.6097711357162225
2211	17	0.3601185337087145
2211	18	0.7242872855655023
2211	19	0.41363282068115126
2211	20	0.4900857993918848
2211	21	0.2841800738494342
2211	22	-0.008217796557488555
2211	23	-0.3459455015614026
2211	24	-0.15306853410296384
2211	25	-0.04039062672229221
2212	1	-0.14938553261142218
2212	2	0.0402609634613178
2212	3	-2.31515458371468
2212	4	-0.966461123120947
2212	5	-0.38368814703506615
2212	6	-0.25170128007415116
2212	7	-0.12800431359255526
2212	8	-0.38451511106016056
2212	9	-0.46772700558747715
2212	10	0.15818993681641302
2212	11	-0.08210488987353415
2212	12	0.2815051686266088
2212	13	-0.09710975349199202
2212	14	0.10022381822356152
2212	15	0.16478298774517697
2212	16	-0.5478697779991677
2212	17	-1.1000612993034906
2212	18	0.02803967983403055
2212	19	0.2699426063479322
2212	20	0.03301212128388187
2212	21	0.3637976729309429
2212	22	0.13320177022910684
2212	23	0.2541221347484921
2212	24	0.10237221023746912
2212	25	0.22453625217460882
2213	1	-2.9938671824028056
2213	2	-1.316786349338205
2213	3	-2.203503109231349
2213	4	-0.9893778451527133
2213	5	-0.9350625302678566
2213	6	-1.017307594353035
2213	7	-0.4958588938365125
2213	8	-0.31591553803580297
2213	9	-0.6227138508398085
2213	10	-0.2212957435542222
2213	11	-0.45964171873779947
2213	12	-0.2327524518257036
2213	13	-0.5740911596769394
2213	14	-0.19918178206700077
2213	15	-0.3619561407638656
2213	16	-1.7414615999095773
2213	17	-1.1401013393435306
2213	18	-0.308615559476074
2213	19	-0.37726212050281305
2213	20	0.08654352879759131
2213	21	-0.08038101903535733
2213	22	0.20109266997841502
2213	23	-0.052724534930035816
2213	24	0.23634689288471122
2213	25	0.27515324084469484
2214	1	-0.5321767585195651
2214	2	-0.5636929913299156
2214	3	-5.958146221152344
2214	4	-1.566983806501586
2214	5	-1.0677490920659487
2214	6	-0.34456680062925216
2214	7	-0.25376803170784257
2214	8	-0.18023921899465023
2214	9	0.2911527567886125
2214	10	0.15610812534303165
2214	11	-0.19465839893405956
2214	12	-0.15314732685195764
2214	13	-4.941906990630766
2214	14	-3.2839205851422832
2214	15	-1.8236276421329052
2214	16	-2.424821708600497
2214	17	-2.877208957358519
2214	18	-0.7442212975392763
2214	19	-0.6464689454752275
2214	20	-0.24684467982691416
2214	21	-0.23150189416379874
2214	22	0.008188991252424671
2214	23	-0.08356255631625942
2214	24	-0.04966880144856134
2214	25	0.13804070297601356
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	187.0
3	39.0
4	29.0
5	45.0
6	9.0
7	9.0
8	17.0
9	51.0
10	161.0
11	490.0
12	1198.0
13	2454.0
14	3940.0
15	5895.0
16	7504.0
17	8547.0
18	8510.0
19	8170.0
20	7531.0
21	7083.0
22	6430.0
23	5850.0
24	5808.0
25	5559.0
26	5792.0
27	6224.0
28	6930.0
29	8308.0
30	10100.0
31	12827.0
32	16813.0
33	22646.0
34	30415.0
35	41646.0
36	58729.0
37	88056.0
38	150210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.30747838370239	21.50446856371405	13.361548096607054	20.826504955976507
2	17.042329131796855	23.37460105013899	37.73440602432675	21.848663793737405
3	19.478002835022643	29.172787459713078	27.666658699676972	23.68255100558731
4	12.677203426132069	20.9060975811537	34.285404375874414	32.13129461683982
5	13.16021693170328	40.659251769464106	30.917547568710358	15.262983730122254
6	27.83702483350556	40.49942816376213	16.697802015952576	14.965744986779733
7	24.9688353809438	37.669474853186145	19.181409339457165	18.180280426412885
8	23.60338662082533	37.57053161857309	19.611329184324468	19.214752576277107
9	24.243254424270283	21.01898414157665	19.93675017237417	34.8010112617789
10	16.61625642845454	32.950092944834346	27.98715136493764	22.44649926177347
11	29.61267417895273	30.768014502425956	20.210182217981316	19.409129100639998
12	20.834076413798684	32.14262732415652	26.597010150632368	20.426286111412427
13	26.82353287258941	27.76487719317599	24.465391806994997	20.946198127239597
14	20.82079247787692	29.128553804176953	25.635377806868682	24.415275911077444
15	23.76994020695894	32.280703689811645	23.70411637946885	20.245239723760562
16	20.517412240140175	33.05742426443145	23.859576869970343	22.565586625458035
17	19.739982275697482	31.54594896724681	25.4579084587579	23.256160298297804
18	19.787555452516454	29.78713225620673	28.39628838436529	22.029023906911533
19	21.153075296497196	30.48230210535993	28.88130918451779	19.48331341362508
20	23.060686792022665	27.22475643097606	29.253104022681015	20.46145275432026
21	24.296010296010294	28.15260158117301	27.466629895201322	20.08475822761537
22	23.512625993329387	28.071368946712315	27.511868470502577	20.904136589455725
23	23.323962514272267	28.254030708584686	27.947533219216492	20.474473557926554
24	22.715980368179387	27.730222448608068	28.02867172602246	21.52512545719009
25	22.35367017432891	29.158032350415642	28.32895411845393	20.15934335680151
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1838.0
1	1838.0
2	1890.0
3	1942.0
4	1942.0
5	1942.0
6	2595.5
7	3249.0
8	3249.0
9	3249.0
10	3874.5
11	4500.0
12	4500.0
13	4500.0
14	5541.5
15	6583.0
16	6583.0
17	6583.0
18	9405.0
19	12227.0
20	12227.0
21	12227.0
22	16368.0
23	20509.0
24	20509.0
25	20509.0
26	26931.5
27	33354.0
28	33354.0
29	33354.0
30	38411.5
31	43469.0
32	43469.0
33	43469.0
34	46436.0
35	49403.0
36	49403.0
37	49403.0
38	49052.0
39	48701.0
40	48701.0
41	48701.0
42	51799.0
43	54897.0
44	54897.0
45	54897.0
46	61238.0
47	67579.0
48	67579.0
49	67579.0
50	65114.0
51	62649.0
52	62649.0
53	62649.0
54	55799.5
55	48950.0
56	48950.0
57	48950.0
58	41767.5
59	34585.0
60	34585.0
61	34585.0
62	30736.0
63	26887.0
64	26887.0
65	26887.0
66	19269.5
67	11652.0
68	11652.0
69	11652.0
70	9400.0
71	7148.0
72	7148.0
73	7148.0
74	4974.5
75	2801.0
76	2801.0
77	2801.0
78	1877.0
79	953.0
80	953.0
81	953.0
82	585.0
83	217.0
84	217.0
85	217.0
86	142.0
87	67.0
88	67.0
89	67.0
90	52.5
91	38.0
92	38.0
93	38.0
94	23.5
95	9.0
96	9.0
97	9.0
98	7.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09646975810897224
2	0.05218554533894879
3	0.05530932798247742
4	0.050531778057080694
5	0.04814300309438234
6	0.06431317207264815
7	0.059903125987666575
8	0.05641183950372281
9	0.061924397109949805
10	0.06265940479078007
11	0.055676831822892556
12	0.05604433566330768
13	0.05677934334413795
14	0.05935187022704388
15	0.06174064518974223
16	0.05751435102496821
17	0.059903125987666575
18	0.13395514983131573
19	0.06651819511513896
20	0.05898436638662874
21	0.05733059910476065
22	0.0613731413493271
23	0.059719374067459
24	0.07368452000323404
25	0.06523193167368599
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	544212.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.61669875443311
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.30532767653044	56.176934292093414
2	8.113977343969554	10.323689047832127
3	1.7440035675494348	3.3284324875034703
4	0.5711612656587899	1.453415767104639
5	0.2861054857972221	0.9100543250976308
6	0.16909032803284488	0.6454181076452261
7	0.12967630911455624	0.5774705084769233
8	0.09319305624175774	0.47429076679494747
9	0.06189359807372021	0.3543719745135438
>10	0.3922769275095479	4.914535104225733
>50	0.05534617118894267	2.4934158946318523
>100	0.06316997372727925	8.229046515321265
>500	0.01130105034570589	4.903877585138352
>1k	0.0031874757385324305	4.05184682810845
>5k	2.897705216847664E-4	1.1632007955124803
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6310	1.1594746165097425	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4008	0.7364776961919252	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3626	0.6662844626726349	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2898	0.5325130647615268	No Hit
ATACAGGGTGACAGCCCCGTACACA	1988	0.3652988173726415	No Hit
GGATACCACGTGTCCCGCCCTACTC	1707	0.3136645277943155	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1612	0.29620809537459664	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	1383	0.25412890564706403	No Hit
ACCCTGTATCGCGCGCCTTTCCAGA	1350	0.24806509228021434	No Hit
GTTAATGATAGTGTGTCGAAACACA	1205	0.22142106385011723	No Hit
GGTATCAACGCAGAGTACATGGGGG	1148	0.21094720439828596	No Hit
GTTCACTATCGGTCAGTCAGGAGTA	1055	0.19385827581898232	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	954	0.17529933187801813	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	928	0.1705217819526214	No Hit
ACTTAGATGTTTCAGTTCCCCCGGT	883	0.16225294554328093	No Hit
GTATCAACGCAGAGTACATGGGGGT	858	0.1576591475380918	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG	837	0.1538003572137329	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	819	0.1504928226499967	No Hit
CCCATATTCAGACAGGATACCACGT	812	0.14920655920854373	No Hit
GCCCAGAGCCTGAATCAGTGTGTGT	804	0.1477365438468832	No Hit
GTCAGGAGTATTTAGCCTTGGAGGA	797	0.14645028040543023	No Hit
AGCGTACACGGTGGATGCCCTGGCA	754	0.1385489478365049	No Hit
GCCTTGGAGGATGGTCCCCCCATAT	754	0.1385489478365049	No Hit
GGTTAATGAGGCGAACCGGGGGAAC	743	0.13652767671422167	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	728	0.13377139791110818	No Hit
TCTAAGTACCCCGAGGAAAAGAAAT	713	0.13101511910799468	No Hit
TATTCAGACAGGATACCACGTGTCC	709	0.13028011142716442	No Hit
ATCATTAACTGAATCCATAGGTTAA	691	0.12697257686342822	No Hit
GGTATCAACGCAGAGTACATGGGGA	687	0.12623756918259796	No Hit
GTACATGGGGGTTAAGCGACTAAGC	676	0.12421629806031473	No Hit
CGGTACTGGTTCACTATCGGTCAGT	673	0.12366504229969204	No Hit
GTGGATGCCCTGGCAGTCAGAGGCG	669	0.12293003461886177	No Hit
GTGGTATCAACGCAGAGTACATGGG	669	0.12293003461886177	No Hit
GTACTTAGATGTTTCAGTTCCCCCG	666	0.12237877885823906	No Hit
TCACGGTACTGGTTCACTATCGGTC	651	0.11962250005512559	No Hit
TCATTAACCTATGGATTCAGTTAAT	646	0.11870374045408776	No Hit
GGGTTTCCCCATTCGGAAATCGCCG	638	0.11723372509242722	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	623	0.11447744628931374	No Hit
CACACACACTGATTCAGGCTCTGGG	617	0.11337493476806831	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	602	0.11061865596495483	No Hit
GGTTCACTATCGGTCAGTCAGGAGT	593	0.10896488868308674	No Hit
CATCTAAGTACCCCGAGGAAAAGAA	578	0.10620860987997326	No Hit
TTCCAGACGCTTCCACTAACACACA	571	0.10492234643852028	No Hit
AGCTCACAGCATGTGCATTTTTGTG	570	0.10473859451831272	No Hit
GTCCCGCCCTACTCATCGAGCTCAC	557	0.10234981955561437	No Hit
ACGCAGAGTACATGGGGGTTAAGCG	552	0.10143105995457653	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.8375192020756616E-4	0.0
4	0.0	0.0	0.0	3.675038404151323E-4	0.0
5	0.0	0.0	0.0	3.675038404151323E-4	0.0
6	0.0	0.0	0.0	5.512557606226985E-4	0.0
7	0.0	0.0	0.0	7.350076808302646E-4	0.0
8	0.0	0.0	0.0	9.187596010378308E-4	0.0
9	0.0	0.0	0.0	9.187596010378308E-4	0.0
10	0.0	0.0	0.0	9.187596010378308E-4	0.0
11	0.0	0.0	0.0	9.187596010378308E-4	0.0
12	0.0	0.0	0.0	9.187596010378308E-4	0.0
13	0.0	0.0	0.0	9.187596010378308E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1280	0.0	15.584881	1
GTATGGA	40	0.0052756327	14.249034	1
AATCGCC	100	9.973519E-9	13.303995	18
ATCGCCG	100	1.002627E-8	13.299099	19
CGAGAGG	85	3.9168226E-6	12.297811	16
AAATCGC	110	3.7771315E-8	12.09454	17
ACGCTTC	120	1.2710916E-7	11.081562	7
AGAGATC	60	0.005874315	11.081562	8
GTACTGG	615	0.0	10.812275	1
TACTGGT	610	0.0	10.7441845	2
AGCGTAC	195	1.8189894E-12	10.717221	1
GAAATCG	125	2.2184759E-7	10.643196	16
ACTGGTT	615	0.0	10.50142	3
ACGGTGG	185	6.002665E-11	10.268629	8
TCCTAGT	75	0.0026362576	10.136376	16
CCGAGAG	75	0.0026362576	10.136376	15
GTATATA	75	0.002644323	10.132646	1
GAATAAG	85	6.5823604E-4	10.058141	1
AGGGTGA	285	0.0	9.998402	5
ACAGGGT	295	0.0	9.980537	3
>>END_MODULE