FastQCFastQC Report
Wed 25 May 2016
SRR1294596_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294596_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544212
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT164753.0273128854196525No Hit
GGTATCAACGCAGAGTACTTTTTTT116402.13887235121607No Hit
TATCAACGCAGAGTACTTTTTTTTT103591.903486141430178No Hit
ACGCAGAGTACTTTTTTTTTTTTTT61191.1243779997500973No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30380.5582383335905861No Hit
GTACTGGTTCACTATCGGTCAGTCA27560.5064202920920523No Hit
GAGTACTTTTTTTTTTTTTTTTTTT26650.4896988673531638No Hit
ATACAGGGTGACAGCCCCGTACACA22400.4116043012649482No Hit
GCAGAGTACTTTTTTTTTTTTTTTT20090.36915760769700046No Hit
ATCAACGCAGAGTACTTTTTTTTTT17820.3274459218098829No Hit
GGATACCACGTGTCCCGCCCTACTC17710.3254246506875997No Hit
ACCCTGTATCGCGCGCCTTTCCAGA14490.2662565323807634No Hit
GTGGTATCAACGCAGAGTACTTTTT13640.2506376191631203No Hit
GGTATCAACGCAGAGTACATGGGGG11610.21333597936098433No Hit
GTTAATGATAGTGTGTCGAAACACA11190.20561839871226653No Hit
ACTTAGATGTTTCAGTTCCCCCGGT10870.1997383372656244No Hit
GTTCACTATCGGTCAGTCAGGAGTA9960.1830169125267359No Hit
CCCATATTCAGACAGGATACCACGT9140.16794925506971548No Hit
GTGTGTGTGTTAGTGGAAGCGTCTG8940.16427421666556416No Hit
AGCGTACACGGTGGATGCCCTGGCA8850.16262044938369605No Hit
CTATCGGTCAGTCAGGAGTATTTAG8480.15582162833601612No Hit
GCCCAGAGCCTGAATCAGTGTGTGT8450.15527037257539342No Hit
TCTAAGTACCCCGAGGAAAAGAAAT8250.1515953341712421No Hit
CAACGCAGAGTACTTTTTTTTTTTT8220.1510440784106194No Hit
GCCTTGGAGGATGGTCCCCCCATAT8080.14847155152771346No Hit
GTCAGGAGTATTTAGCCTTGGAGGA8040.1477365438468832No Hit
GTATTTAGCCTTGGAGGATGGTCCC7920.1455315208043924No Hit
GTATCAACGCAGAGTACATGGGGGT7830.1438777535225243No Hit
CACACACACTGATTCAGGCTCTGGG7570.1391002035971276No Hit
CGGTACTGGTTCACTATCGGTCAGT7470.13726268439505193No Hit
ATCATTAACTGAATCCATAGGTTAA7350.13505766135256114No Hit
TCACGGTACTGGTTCACTATCGGTC7320.13450640559193844No Hit
GTACTTAGATGTTTCAGTTCCCCCG7150.1313826229484098No Hit
GGTTAATGAGGCGAACCGGGGGAAC7140.13119887102820224No Hit
GGTATCAACGCAGAGTACATGGGGA7030.129177599905919No Hit
TCATTAACCTATGGATTCAGTTAAT6740.12384879421989961No Hit
TATTCAGACAGGATACCACGTGTCC6740.12384879421989961No Hit
AACGCAGAGTACTTTTTTTTTTTTT6670.12256253077844663No Hit
ACGCAGAGTACATGGGGGTTAAGCG6650.1221950269380315No Hit
GTGGTATCAACGCAGAGTACATGGG6640.12201127501782394No Hit
CATCTAAGTACCCCGAGGAAAAGAA6630.12182752309761638No Hit
GTGGATGCCCTGGCAGTCAGAGGCG6560.1205412596561634No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA6400.11760122893284236No Hit
AGCTCACAGCATGTGCATTTTTGTG6350.11668246933180453No Hit
GGGTTTCCCCATTCGGAAATCGCCG6270.11521245397014399No Hit
CCCCAGTAGCGGCGAGCGAACGGGG6110.11227242324682292No Hit
GGTTCACTATCGGTCAGTCAGGAGT6050.11116991172557752No Hit
GTACATGGGGGTTAAGCGACTAAGC5790.10639236180018082No Hit
CCTATGGATTCAGTTAATGATAGTG5650.10381983491727488No Hit
TCAACGCAGAGTACTTTTTTTTTTT5650.10381983491727488No Hit
ATGATAGTGTGTCGAAACACACTGG5490.10087980419395381No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC402.752282E-416.6287083
TAATACC350.002132761316.325434
GGTATCA21300.015.9363591
CTATACT551.09991815E-515.5833654
ATCGCCG1400.013.54327219
AATCGCC1501.8189894E-1212.64038818
AGGACCG550.003008621512.1203965
AACACTA550.003008621512.1203965
CAGGACA802.787922E-511.903964
GAAATCG1603.6379788E-1211.85036416
AAATCGC1603.6379788E-1211.85036417
AGTGTCC657.846638E-411.7208218
GTGTAAA657.950571E-411.7024591
AAAACCG600.00576186711.1103635
AGACCAA600.00576186711.1103636
CATGCAA700.001459865110.88261610
TACAGGG4000.010.6898832
ACAGGGT4050.010.5579093
GTATCAA45400.010.3669221
ATACAGG4350.010.2732791
Produced by FastQC (version 0.11.5)