FastQCFastQC Report
Wed 25 May 2016
SRR1294595_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294595_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315789
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT102340.7777842800023408No Hit
GGTATCAACGCAGAGTACTTTTTTT61010.46367616692342006No Hit
TATCAACGCAGAGTACTTTTTTTTT57110.4340361562530162No Hit
GTACTGGTTCACTATCGGTCAGTCA30150.22914008249042972No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA28250.2147000772920278No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28060.21325607677218764No Hit
ATACAGGGTGACAGCCCCGTACACA19290.146604052777459No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17490.13292404785265724No Hit
GTTAATGATAGTGTGTCGAAACACA17480.13284804782529722No Hit
CCCATGTACTCTGCGTTGATACCAC17060.1296560466761768No Hit
GTACATGGGAAGCAGTGGTATCAAC16030.1218280438580958No Hit
GGATACCACGTGTCCCGCCCTACTC15340.11658404197025511No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14450.10982003953521424No Hit
GGTATCAACGCAGAGTACATGGGGG13570.10313203712753337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG1102.748493E-912.9530671
GGTATCA22750.011.0229181
GCTATAC700.001495402210.8534273
GCTACAC1053.4785207E-610.8534273
AGGGTGA4050.010.5519425
ACGCTTC1451.4493708E-810.4791717
ACCGTGT1002.4066761E-510.4464248
TCTATAC1002.4066761E-510.4464243
TCTAGAC1106.066495E-610.3600893
ATTTTCG750.002635432210.13873315
GTACAAG1502.445995E-810.1321771
CTAGTCC750.002654634610.1298663
CTACACT1502.4547262E-810.1298664
GTCCTGG1701.5588739E-910.0576761
GTGTAGG951.6452782E-49.9988591
CGCCTTT3350.09.93066114
GTATCAA44550.09.8933721
GGACAGA1252.579136E-69.8766186
GTATAGA1356.4466076E-79.8507281
AATCGCC1554.017602E-89.812798518
Produced by FastQC (version 0.11.5)