##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294595_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1315789 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.24100824676297 31.0 28.0 33.0 22.0 34.0 2 29.26546049556578 31.0 28.0 34.0 22.0 34.0 3 29.271822457856086 31.0 28.0 34.0 20.0 34.0 4 32.630125346845126 35.0 33.0 37.0 20.0 37.0 5 33.25179645064672 35.0 33.0 37.0 26.0 37.0 6 33.1324422076792 35.0 33.0 37.0 25.0 37.0 7 33.074829626938666 35.0 33.0 37.0 25.0 37.0 8 32.87668767560756 35.0 33.0 37.0 23.0 37.0 9 33.74891262960855 37.0 33.0 39.0 18.0 39.0 10 33.97299339027762 37.0 33.0 39.0 21.0 39.0 11 34.169668541080675 38.0 33.0 39.0 23.0 39.0 12 34.00151012054364 37.0 33.0 39.0 22.0 39.0 13 33.75562267202416 37.0 33.0 39.0 18.0 39.0 14 34.556517800346406 38.0 33.0 41.0 17.0 41.0 15 34.53049919097971 38.0 32.0 40.0 18.0 41.0 16 34.698035931292935 38.0 33.0 40.0 19.0 41.0 17 34.74625262865094 38.0 33.0 40.0 20.0 41.0 18 34.667546240316646 38.0 33.0 40.0 19.0 41.0 19 34.98821087575592 39.0 33.0 41.0 22.0 41.0 20 35.10365491731577 39.0 33.0 41.0 23.0 41.0 21 35.14820537335393 39.0 33.0 41.0 23.0 41.0 22 35.196469190728905 39.0 33.0 41.0 24.0 41.0 23 35.21540459754566 39.0 33.0 41.0 24.0 41.0 24 35.28842618383343 39.0 33.0 41.0 25.0 41.0 25 34.06302454268884 38.0 31.0 40.0 20.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0757719550254663 1101 2 0.9519847847098362 1101 3 1.2753175257129037 1101 4 1.0860566198379757 1101 5 0.7884228287252029 1101 6 0.8010744777832954 1101 7 0.7639704367022162 1101 8 0.9231034249415941 1101 9 1.286366552904667 1101 10 1.0955041571202386 1101 11 1.0261178719727582 1101 12 1.1146170662073374 1101 13 1.2654172043348026 1101 14 1.1327820705962992 1101 15 1.4216937564896028 1101 16 1.3690563991659914 1101 17 1.143052724613213 1101 18 1.287397639787919 1101 19 1.203173062243863 1101 20 1.1002371686497199 1101 21 0.8597166221550978 1101 22 0.8568123414385553 1101 23 1.03086486239836 1101 24 0.9989316512439501 1101 25 1.1023285092503698 1102 1 1.03082639367679 1102 2 0.9733690988720589 1102 3 1.2404951116126988 1102 4 1.1220706936146172 1102 5 0.8133104316161095 1102 6 0.90801852631337 1102 7 0.8228916901006897 1102 8 1.0438717814899832 1102 9 1.3323564767562814 1102 10 1.089763994783425 1102 11 0.8498523678809633 1102 12 1.0200320168193642 1102 13 1.16361714773182 1102 14 1.4674574259230582 1102 15 1.3920201023174883 1102 16 1.282691894345028 1102 17 1.2728731310444275 1102 18 1.378725633641288 1102 19 1.1687518413699607 1102 20 0.4396381238165432 1102 21 0.7877409226127199 1102 22 0.773318854357619 1102 23 0.8835121757490043 1102 24 0.854233208575252 1102 25 1.0467875982692902 1103 1 0.8022649694479078 1103 2 0.8068940299558562 1103 3 1.0288979532017777 1103 4 0.7846110176223604 1103 5 0.6590331702274028 1103 6 0.6278795666039514 1103 7 0.44941092699546914 1103 8 0.5737043377273068 1103 9 0.5646204674337554 1103 10 0.6204607071233283 1103 11 0.6111861831580114 1103 12 0.8415462595013992 1103 13 0.6480588969908823 1103 14 0.7352679879620609 1103 15 0.9413459819084551 1103 16 0.7505819574346546 1103 17 0.8523165057165656 1103 18 0.970681411919216 1103 19 0.7348374993280018 1103 20 -0.11827098544398496 1103 21 0.42029008152699987 1103 22 -0.1622697286885355 1103 23 0.21437024966073182 1103 24 0.46661479264515293 1103 25 0.6916676126358396 1104 1 0.3204921981368827 1104 2 0.4237684850616823 1104 3 0.7016878049905628 1104 4 0.6220698035183645 1104 5 0.29938131532147594 1104 6 0.28159014434814367 1104 7 0.260372219245113 1104 8 0.32531843713132247 1104 9 0.5399287036168943 1104 10 0.6212974348096338 1104 11 0.3882644717658721 1104 12 0.5090152954711158 1104 13 0.48269173245741115 1104 14 0.745534177142936 1104 15 0.6242177321691784 1104 16 0.4207885481171729 1104 17 0.4694277464605392 1104 18 0.6032577545448419 1104 19 -0.09743322388299447 1104 20 0.13313169821167747 1104 21 -0.4205772049095913 1104 22 -0.16500281574778342 1104 23 -0.21070273265119255 1104 24 -0.675058969080844 1104 25 -1.8026259173082906 1105 1 -0.14113901528867956 1105 2 -0.03377271591498854 1105 3 0.41712217639403804 1105 4 0.3512507931934934 1105 5 0.15119706666411048 1105 6 0.02443731475255362 1105 7 -0.18634934367841538 1105 8 0.06044817736695052 1105 9 0.5178705214075663 1105 10 -1.6428439977325269 1105 11 -0.6359674167357028 1105 12 0.1236060663198586 1105 13 0.25433317722292514 1105 14 0.49661925593150613 1105 15 -0.3264399866883636 1105 16 0.034256347786048025 1105 17 -0.4156344183534557 1105 18 -0.5459431615599044 1105 19 -1.6148005567460046 1105 20 -0.5027761254118133 1105 21 -1.7112310091400644 1105 22 -0.9142870399111303 1105 23 -0.6378092065642704 1105 24 -0.8764127486911235 1105 25 -0.9092991893166698 1106 1 -0.4698249745318215 1106 2 -0.5549628097485844 1106 3 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1109 1 -0.1928521208875864 1109 2 -0.32402631221486544 1109 3 0.09696535598039802 1109 4 0.1270395803839932 1109 5 0.15303183551891664 1109 6 0.11635071870005476 1109 7 -0.2046917848895049 1109 8 -0.2754596494109549 1109 9 0.14732082537830138 1109 10 0.12555444006029148 1109 11 -0.09628120748689639 1109 12 -0.0025264214426030662 1109 13 0.08589575921256198 1109 14 0.5740191254976992 1109 15 -0.5530430856156556 1109 16 -0.2393807313962668 1109 17 0.06506782525790555 1109 18 0.24732765350145058 1109 19 0.18029858044187108 1109 20 0.07973150196728795 1109 21 0.2615750417890865 1109 22 0.0940989541968662 1109 23 0.06581355488429352 1109 24 0.15546512328884887 1109 25 0.03549822599506314 1110 1 -0.32437918491849516 1110 2 -0.03160424086935265 1110 3 0.031507536857397156 1110 4 -0.12039655750565004 1110 5 -0.2978192841779972 1110 6 -0.16592768634139787 1110 7 -0.2870617162447644 1110 8 -0.28078768122010445 1110 9 0.04687069596609916 1110 10 -0.0425944027320071 1110 11 -0.38349828239593364 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0.1987496913293043 2207 12 0.55656426033174 2207 13 0.7043833246716176 2207 14 0.9161703975287381 2207 15 0.7939012536497714 2207 16 0.9145388102296224 2207 17 0.7775646639339371 2207 18 0.9099071509759611 2207 19 -0.2545599994271015 2207 20 0.3339569597341452 2207 21 -0.28687287316594023 2207 22 0.33626637219890654 2207 23 -0.49557714189294444 2207 24 -1.6897294499502777 2207 25 -0.3776754575010415 2208 1 0.46657609803989786 2208 2 0.040384570344535575 2208 3 0.7016388721078286 2208 4 0.38616333156279836 2208 5 0.440517175829342 2208 6 0.19478470223582178 2208 7 0.29692627613167843 2208 8 0.3693505723102035 2208 9 -0.2222090009743951 2208 10 0.14738649355131628 2208 11 0.18398341143208086 2208 12 0.13333965245499968 2208 13 0.44371102230701354 2208 14 0.6319824390484285 2208 15 0.6340595073944897 2208 16 0.4527351563746862 2208 17 0.31903476916465223 2208 18 0.6225167227263881 2208 19 -1.5197692051118636 2208 20 -0.18247167033990053 2208 21 -0.24137036815043444 2208 22 0.07450708562709707 2208 23 0.3649893506200428 2208 24 0.2942495212666216 2208 25 0.42407848735233244 2209 1 0.23248276374612686 2209 2 -1.2572868465724305 2209 3 -0.1784121426879146 2209 4 0.12089979513630311 2209 5 0.2123637483844334 2209 6 0.2319774533996508 2209 7 0.18011294751118356 2209 8 0.34953292116853873 2209 9 -1.5872932652886647 2209 10 -0.1524749411951376 2209 11 0.31128783005269867 2209 12 0.5398437038763007 2209 13 0.7690360210645935 2209 14 0.7493904563452176 2209 15 0.9207162011599763 2209 16 0.7021718773115779 2209 17 0.5359868305566238 2209 18 0.7281084435432277 2209 19 0.5167846047206623 2209 20 0.5041084118890709 2209 21 0.5436841561460923 2209 22 0.5751665479056882 2209 23 0.40012009557788986 2209 24 0.5113621206815395 2209 25 0.6306794758198393 2210 1 0.11300392383638069 2210 2 0.19751806234837588 2210 3 -0.021727865543013536 2210 4 0.3081646677354968 2210 5 0.047036028391033824 2210 6 0.10398174595372467 2210 7 0.0657554548030177 2210 8 0.10342923947793281 2210 9 0.20718805109458316 2210 10 -1.518561562734952 2210 11 -0.5229408860333677 2210 12 -2.2523587065579065 2210 13 -0.8395479864198663 2210 14 -0.6261485893869931 2210 15 -0.7152653078378819 2210 16 -0.2543632610957758 2210 17 9.029056212881414E-4 2210 18 0.2663143760730833 2210 19 0.1486144434747203 2210 20 0.2879425431971967 2210 21 0.22501319692535304 2210 22 0.19363773826395203 2210 23 0.08575764992272639 2210 24 0.05052452231051063 2210 25 -0.11949827994484252 2211 1 -0.2388479280154705 2211 2 0.03630478010061111 2211 3 -1.432238721226284 2211 4 -0.6112095340524952 2211 5 -0.4126574581989999 2211 6 -0.4459799398700355 2211 7 -0.4083569334473012 2211 8 -0.1358427911734097 2211 9 0.18444220179701176 2211 10 0.0072749634463988855 2211 11 -0.2664785616400138 2211 12 -0.23699472188357618 2211 13 -0.3790243593445126 2211 14 -0.6781792407279923 2211 15 -0.2186369553474563 2211 16 -0.1882074501123796 2211 17 -0.17830526806964286 2211 18 0.00671028922761252 2211 19 0.033493115249939365 2211 20 0.22772542953180874 2211 21 0.014744997691636286 2211 22 -0.09770325790463374 2211 23 -0.38724362721647765 2211 24 -0.35408595023099565 2211 25 -0.3412862748362855 2212 1 -0.6081989566629815 2212 2 -0.3839389377518394 2212 3 -2.467791373968332 2212 4 -1.7435470246294607 2212 5 -0.8079586028850017 2212 6 -0.7193279649941786 2212 7 -0.64085007999347 2212 8 -0.4837008554411071 2212 9 -1.0952877180646041 2212 10 -0.45535679244476057 2212 11 -0.5117291292437258 2212 12 -0.23487685284489856 2212 13 -0.7896436212893647 2212 14 -0.355880153188906 2212 15 -0.12360358039725838 2212 16 -1.3825927275161973 2212 17 -1.6071667875740303 2212 18 -0.461946730027222 2212 19 -0.32848787724569206 2212 20 -0.29411231800339266 2212 21 -0.1609825939950582 2212 22 -0.49695282137183483 2212 23 -0.2594395612041325 2212 24 -0.3262171633256514 2212 25 -0.24101583925134662 2213 1 -3.00731443222649 2213 2 -1.440699343206468 2213 3 -2.3964008879305396 2213 4 -1.1039563759707711 2213 5 -0.8217459817838204 2213 6 -0.44440182037520515 2213 7 -0.3333457664644186 2213 8 -0.2713965482181919 2213 9 -0.37972586166388567 2213 10 -0.13023799099083533 2213 11 -0.6538030409932531 2213 12 -0.06274261489872401 2213 13 -0.6719295291784704 2213 14 -0.20761924049212155 2213 15 -0.2164059174740629 2213 16 -1.392577389036724 2213 17 -1.043823983638113 2213 18 -0.2745613168572518 2213 19 -0.2026494329449875 2213 20 -0.12996961675035834 2213 21 0.05653899744692126 2213 22 -0.04036422830439079 2213 23 0.07559191666349818 2213 24 0.13305897288266522 2213 25 0.05534446584057662 2214 1 -0.906721552438615 2214 2 -0.6352307247774078 2214 3 -6.439241769223052 2214 4 -2.109713689855319 2214 5 -1.2826267334123003 2214 6 -1.0308709342024756 2214 7 -0.5692948351034985 2214 8 -0.2947248251298049 2214 9 -0.2062387623002806 2214 10 -0.2570291997009946 2214 11 -0.7061594400506479 2214 12 -0.6220001691067196 2214 13 -5.239887591200361 2214 14 -4.053344187541057 2214 15 -2.3300718396092677 2214 16 -2.4607057057768813 2214 17 -3.024885758442135 2214 18 -1.0994340560651281 2214 19 -0.5984722918228798 2214 20 -0.1791086933428545 2214 21 -0.18468151375977016 2214 22 -0.1916054282252233 2214 23 -0.12132671879103185 2214 24 -0.11877141899821453 2214 25 0.04872204901030841 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 500.0 3 78.0 4 69.0 5 128.0 6 23.0 7 25.0 8 68.0 9 220.0 10 551.0 11 1393.0 12 2842.0 13 5065.0 14 8229.0 15 11685.0 16 15173.0 17 17125.0 18 17846.0 19 17435.0 20 16600.0 21 15701.0 22 15325.0 23 14748.0 24 14415.0 25 14762.0 26 15554.0 27 16619.0 28 18871.0 29 22404.0 30 27486.0 31 33952.0 32 44081.0 33 57211.0 34 76020.0 35 101203.0 36 140567.0 37 205011.0 38 366804.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.687741923704266 21.593212138179517 13.582646614893978 22.136399323222243 2 15.831988672915218 23.146003507016314 38.40296925353477 22.619038566533696 3 18.719919819136795 28.339874390411758 28.989975110777195 23.95023067967426 4 12.507518411077442 19.995209507986054 35.664984925158066 31.832287155778438 5 12.713396737725411 40.24245032528461 32.84325205526949 14.200900881720496 6 28.669622031231345 39.719156709612996 17.237319729225938 14.37390152992972 7 24.915395507938754 36.626178188847874 20.827078304594423 17.631347998618953 8 23.7597500222434 37.483355475335685 20.33548365509579 18.421410847325127 9 24.20430702025898 20.192817374097192 20.773535788787633 34.8293398168562 10 15.462952400508012 32.277991984364185 30.605887765888678 21.65316784923912 11 30.11049345632353 29.209435669690727 21.94074569775135 18.739325176234402 12 20.783805261616966 30.54445073935642 28.668321932783016 20.003422066243598 13 26.048209061575793 26.691412271356015 25.56599677105601 21.694381896012185 14 20.519850093843445 27.225929993094827 26.79412847273977 25.460091440321957 15 22.836874470502522 32.37049081088974 24.06462416896464 20.7280105496431 16 20.494196878740546 32.85178048064537 25.3272668371126 21.32675580350149 17 19.594251349098673 31.46213702097111 27.072395037993786 21.871216591936435 18 19.938427582007765 30.029682624248423 28.99101910343253 21.040870690311287 19 20.743838610612965 30.32964174732029 29.397923159644744 19.528596482421996 20 22.117099338463493 28.579989338151695 29.069886818624624 20.233024504760188 21 22.56018640332533 28.764302303121376 28.53479625064069 20.140715042912614 22 22.160495235487822 28.601826713209068 28.310176207098475 20.927501844204635 23 21.918946872100626 28.691499231904118 28.639101403866334 20.750452492128918 24 21.723165753894524 28.52351468864722 28.52184150543899 21.231478052019266 25 21.619886803530658 28.968607068923298 28.697480078060238 20.71402604948581 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4058.0 1 4058.0 2 4025.5 3 3993.0 4 3993.0 5 3993.0 6 5373.5 7 6754.0 8 6754.0 9 6754.0 10 8388.0 11 10022.0 12 10022.0 13 10022.0 14 13120.5 15 16219.0 16 16219.0 17 16219.0 18 23928.0 19 31637.0 20 31637.0 21 31637.0 22 43581.0 23 55525.0 24 55525.0 25 55525.0 26 71307.0 27 87089.0 28 87089.0 29 87089.0 30 100973.0 31 114857.0 32 114857.0 33 114857.0 34 122859.5 35 130862.0 36 130862.0 37 130862.0 38 130727.0 39 130592.0 40 130592.0 41 130592.0 42 133152.5 43 135713.0 44 135713.0 45 135713.0 46 143651.5 47 151590.0 48 151590.0 49 151590.0 50 144008.0 51 136426.0 52 136426.0 53 136426.0 54 121999.5 55 107573.0 56 107573.0 57 107573.0 58 93338.5 59 79104.0 60 79104.0 61 79104.0 62 68125.5 63 57147.0 64 57147.0 65 57147.0 66 43492.5 67 29838.0 68 29838.0 69 29838.0 70 22732.5 71 15627.0 72 15627.0 73 15627.0 74 11367.0 75 7107.0 76 7107.0 77 7107.0 78 4890.5 79 2674.0 80 2674.0 81 2674.0 82 1824.0 83 974.0 84 974.0 85 974.0 86 612.0 87 250.0 88 250.0 89 250.0 90 184.5 91 119.0 92 119.0 93 119.0 94 69.5 95 20.0 96 20.0 97 20.0 98 19.5 99 19.0 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.10070003625201306 2 0.05403601945296701 3 0.05791202084832751 4 0.05198401871424674 5 0.04810801731888623 6 0.06779202440512878 7 0.06437202317392834 8 0.0601920216691278 9 0.06581602369376853 10 0.06528402350224846 11 0.05920402131344767 12 0.06034402172384782 13 0.061408022106887956 14 0.06330802279088821 15 0.0655880236116885 16 0.061332022079527954 17 0.06361202290032825 18 0.1435640516830586 19 0.06893202481552894 20 0.062320022435208074 21 0.0601160216417678 22 0.06528402350224846 23 0.06452402322864836 24 0.07068002544480916 25 0.06893202481552894 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1315789.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.69041134077415 #Duplication Level Percentage of deduplicated Percentage of total 1 88.08697131388554 62.269042359415415 2 8.330043931841253 11.777084640571555 3 1.7438054291759129 3.6981096926016144 4 0.6099827646454409 1.7247973017427536 5 0.3170654997193567 1.1206745298564718 6 0.1937028753072053 0.8215761560014218 7 0.1198441128075461 0.5930280741794902 8 0.08771578880904055 0.49605321535932406 9 0.06813640940743937 0.43349317274657545 >10 0.3697948786096652 4.873905345229062 >50 0.03461460228777428 1.6797204310699767 >100 0.03202582940356719 4.573640481437087 >500 0.004342458000156 2.230905508882276 >1k 0.0016284217500584998 2.0161031518350967 >5k 2.1712290000779996E-4 0.9064828300969193 >10k+ 1.0856145000389998E-4 0.7853831089749299 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 10234 0.7777842800023408 No Hit GGTATCAACGCAGAGTACTTTTTTT 6101 0.46367616692342006 No Hit TATCAACGCAGAGTACTTTTTTTTT 5711 0.4340361562530162 No Hit GTACTGGTTCACTATCGGTCAGTCA 3015 0.22914008249042972 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 2825 0.2147000772920278 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2806 0.21325607677218764 No Hit ATACAGGGTGACAGCCCCGTACACA 1929 0.146604052777459 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1749 0.13292404785265724 No Hit GTTAATGATAGTGTGTCGAAACACA 1748 0.13284804782529722 No Hit CCCATGTACTCTGCGTTGATACCAC 1706 0.1296560466761768 No Hit GTACATGGGAAGCAGTGGTATCAAC 1603 0.1218280438580958 No Hit GGATACCACGTGTCCCGCCCTACTC 1534 0.11658404197025511 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1445 0.10982003953521424 No Hit GGTATCAACGCAGAGTACATGGGGG 1357 0.10313203712753337 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 1.520000547200197E-4 0.0 6 0.0 0.0 0.0 1.520000547200197E-4 0.0 7 0.0 0.0 0.0 1.520000547200197E-4 0.0 8 0.0 0.0 0.0 1.520000547200197E-4 0.0 9 0.0 0.0 0.0 1.520000547200197E-4 0.0 10 0.0 0.0 0.0 2.2800008208002955E-4 0.0 11 0.0 0.0 0.0 2.2800008208002955E-4 0.0 12 0.0 0.0 0.0 2.2800008208002955E-4 0.0 13 0.0 0.0 0.0 3.040001094400394E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 110 2.748493E-9 12.953067 1 GGTATCA 2275 0.0 11.022918 1 GCTATAC 70 0.0014954022 10.853427 3 GCTACAC 105 3.4785207E-6 10.853427 3 AGGGTGA 405 0.0 10.551942 5 ACGCTTC 145 1.4493708E-8 10.479171 7 ACCGTGT 100 2.4066761E-5 10.446424 8 TCTATAC 100 2.4066761E-5 10.446424 3 TCTAGAC 110 6.066495E-6 10.360089 3 ATTTTCG 75 0.0026354322 10.138733 15 GTACAAG 150 2.445995E-8 10.132177 1 CTAGTCC 75 0.0026546346 10.129866 3 CTACACT 150 2.4547262E-8 10.129866 4 GTCCTGG 170 1.5588739E-9 10.057676 1 GTGTAGG 95 1.6452782E-4 9.998859 1 CGCCTTT 335 0.0 9.930661 14 GTATCAA 4455 0.0 9.893372 1 GGACAGA 125 2.579136E-6 9.876618 6 GTATAGA 135 6.4466076E-7 9.850728 1 AATCGCC 155 4.017602E-8 9.8127985 18 >>END_MODULE