FastQCFastQC Report
Wed 25 May 2016
SRR1294595_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294595_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315789
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT273322.077232747803789No Hit
GGTATCAACGCAGAGTACTTTTTTT172551.31138047209697No Hit
TATCAACGCAGAGTACTTTTTTTTT168311.279156460496326No Hit
ACGCAGAGTACTTTTTTTTTTTTTT97760.7429762674714563No Hit
GTACTTTTTTTTTTTTTTTTTTTTT52360.3979361432570116No Hit
GAGTACTTTTTTTTTTTTTTTTTTT44210.33599612095860354No Hit
GCAGAGTACTTTTTTTTTTTTTTTT32990.2507240902606725No Hit
ATCAACGCAGAGTACTTTTTTTTTT32430.24646808872851195No Hit
GTACTGGTTCACTATCGGTCAGTCA28760.21857607868738835No Hit
ATACAGGGTGACAGCCCCGTACACA20420.15519205586914012No Hit
GTGGTATCAACGCAGAGTACTTTTT20300.15428005554082No Hit
CCCATGTACTCTGCGTTGATACCAC20190.15344405523985988No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA18680.1419680511084984No Hit
GTTAATGATAGTGTGTCGAAACACA17070.1297320467035368No Hit
GGATACCACGTGTCCCGCCCTACTC16830.1279080460468966No Hit
GTACATGGGAAGCAGTGGTATCAAC16560.1258560453081763No Hit
CAACGCAGAGTACTTTTTTTTTTTT14820.11263204054753459No Hit
GGTATCAACGCAGAGTACATGGGGG14580.11080803989089437No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA350.002197496616.25515
GGTATCA36200.013.2635531
GTCTAAG604.0632795E-412.676981
GAAATCG1551.8189894E-1212.2354116
CCAAGAC1405.2750693E-1112.2144573
AATCGCC1501.4551915E-1112.01109418
TCAGAAC1459.640644E-1111.7932693
ATCGCCG1451.0004442E-1011.77130819
ACCGGGG2050.011.56351615
TAATACC1251.770968E-811.4253914
CTAAGAC1251.8297214E-811.400163
ACTGATC1104.824542E-711.2522788
CGGGGGA2050.011.10139617
CTAGGAC951.3594274E-511.00015453
CTAGACA1303.1579475E-810.9859524
CAGTACT700.001463712410.8813244
ACGCTTC1158.561783E-710.7630487
TGACTAT1252.1706364E-710.6636986
CAAGACA1701.3278623E-1010.64129454
GAACCGG2050.010.63843413
Produced by FastQC (version 0.11.5)