##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294595_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1315789 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.905107885838838 31.0 30.0 33.0 28.0 34.0 2 31.08482363053651 31.0 31.0 34.0 28.0 34.0 3 31.15335969520949 31.0 31.0 34.0 28.0 34.0 4 34.72678294164186 37.0 35.0 37.0 32.0 37.0 5 35.01951908702687 37.0 35.0 37.0 32.0 37.0 6 34.39170718101459 37.0 35.0 37.0 30.0 37.0 7 34.234808924531215 36.0 35.0 37.0 30.0 37.0 8 34.233352764006995 37.0 35.0 37.0 30.0 37.0 9 35.55805756090072 39.0 35.0 39.0 29.0 39.0 10 35.372959494265416 39.0 35.0 39.0 28.0 39.0 11 35.528450230242086 39.0 35.0 39.0 29.0 39.0 12 35.532713071776705 39.0 35.0 39.0 29.0 39.0 13 35.31196339230682 39.0 35.0 39.0 27.0 39.0 14 36.26617185582187 39.0 36.0 41.0 27.0 41.0 15 36.29244430527995 39.0 36.0 41.0 27.0 41.0 16 36.36190377028536 39.0 36.0 41.0 27.0 41.0 17 36.48896517602746 39.0 36.0 41.0 28.0 41.0 18 36.537344513444026 39.0 36.0 41.0 28.0 41.0 19 36.58058548901078 39.0 36.0 41.0 28.0 41.0 20 36.66634391988381 39.0 36.0 41.0 29.0 41.0 21 36.516448305921394 39.0 36.0 41.0 27.0 41.0 22 36.50795530286391 39.0 36.0 41.0 28.0 41.0 23 36.51534174552303 39.0 36.0 41.0 28.0 41.0 24 36.42369255252932 39.0 35.0 41.0 27.0 41.0 25 35.23625672505242 38.0 33.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.410915709936301 1101 2 0.41814425031606106 1101 3 0.36291709509256265 1101 4 0.2541226831305252 1101 5 0.3460184787385714 1101 6 0.42975255512430977 1101 7 0.4257510898203378 1101 8 0.4500159715026726 1101 9 0.633365933763713 1101 10 0.7027860744818497 1101 11 0.6183585308866952 1101 12 0.6833923698324185 1101 13 0.590496769275326 1101 14 0.9137659183583438 1101 15 0.8945953079445914 1101 16 0.9097636616482845 1101 17 0.8655926835379049 1101 18 0.8847330227626387 1101 19 1.147034932750337 1101 20 0.996252864047861 1101 21 1.1370098136468272 1101 22 1.002366009220772 1101 23 1.1007071731817746 1101 24 1.1722487681101512 1101 25 1.1438035500271653 1102 1 0.3750693676231194 1102 2 0.2964148009963381 1102 3 0.27409366404586066 1102 4 0.17588404399974422 1102 5 0.28203054314336384 1102 6 0.2447299360681754 1102 7 0.27339421584439094 1102 8 0.30453489552832025 1102 9 0.5837174205796813 1102 10 0.5482068011865024 1102 11 0.48099463602928694 1102 12 0.5754536822383116 1102 13 0.5483246519320986 1102 14 0.8375632156510449 1102 15 0.7955749959197931 1102 16 0.8418250966599174 1102 17 0.6091857673481229 1102 18 0.5890311372566472 1102 19 0.9162342253152218 1102 20 0.8276165021143527 1102 21 1.0958011730754293 1102 22 1.1412255238178375 1102 23 1.0388513854586279 1102 24 1.1140086577104995 1102 25 1.0771254401581203 1103 1 0.2650254618079444 1103 2 0.27305718914760035 1103 3 0.3102569748025559 1103 4 0.3392947504907795 1103 5 0.23797808031486056 1103 6 0.3673521140430438 1103 7 0.3589848306568868 1103 8 0.37649481598794665 1103 9 0.2895525551940352 1103 10 0.5420607080110074 1103 11 0.5673568304942336 1103 12 0.4699766628264257 1103 13 0.6526450391998679 1103 14 0.67822194051449 1103 15 0.7369688597232837 1103 16 0.7378373292040976 1103 17 0.7540074503584933 1103 18 0.7237873842827298 1103 19 0.8224362576058866 1103 20 0.7403703254398124 1103 21 0.9902445215312241 1103 22 0.8268626070927283 1103 23 0.7827959025077647 1103 24 1.0461239034296952 1103 25 0.8795808953196556 1104 1 0.20135996268806977 1104 2 0.22621112917126496 1104 3 0.09447936055508066 1104 4 0.14754337924026117 1104 5 0.07952652435877638 1104 6 0.15924865212213035 1104 7 0.3499513947377153 1104 8 0.1878717595768009 1104 9 0.008713459651346511 1104 10 0.36117977608400764 1104 11 0.44348954022822795 1104 12 0.45926051710943483 1104 13 -0.02676475823671609 1104 14 0.30571824193558683 1104 15 0.4516222208667031 1104 16 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0.19463643754053095 2207 5 0.16594599537187804 2207 6 0.3118558950087973 2207 7 0.24515271138022854 2207 8 0.3247958468505132 2207 9 0.33476571619340945 2207 10 0.622152836711166 2207 11 0.4370870067526198 2207 12 0.3181026467236876 2207 13 0.4828866167958097 2207 14 0.5794258715987652 2207 15 0.6919273671092867 2207 16 0.6158754639357795 2207 17 0.5073138930364678 2207 18 0.5601505834509268 2207 19 0.6448171580978794 2207 20 0.5615930730836354 2207 21 0.9051321578767073 2207 22 0.7934744758309407 2207 23 0.6661525612322379 2207 24 0.9577617111391916 2207 25 0.8543929732254512 2208 1 0.08148305275784296 2208 2 0.04125124742175501 2208 3 0.048857801801087675 2208 4 0.02599081644765988 2208 5 0.092603091341104 2208 6 0.17962516976877652 2208 7 0.09777265067474872 2208 8 -0.11326134994826731 2208 9 0.1635263592543481 2208 10 0.036362405388032926 2208 11 -0.28559385289123895 2208 12 -0.5385934477743604 2208 13 -0.24802239598091802 2208 14 -0.6932763961841175 2208 15 -0.03259819252963325 2208 16 0.14020824360298434 2208 17 0.053753333189085595 2208 18 0.17968875105367488 2208 19 0.34560209473647774 2208 20 0.3054041714784006 2208 21 0.6061679589160534 2208 22 0.6392438106390088 2208 23 0.48775097584890403 2208 24 0.41893557155243144 2208 25 0.5060931522740475 2209 1 0.012135512786667135 2209 2 -0.0028866578111141905 2209 3 -0.03451531704228117 2209 4 -0.5733068366818799 2209 5 0.008415556127438606 2209 6 0.1271017354053754 2209 7 0.07041096883933307 2209 8 0.15235564932526557 2209 9 0.21199334677370274 2209 10 0.31089345288327763 2209 11 0.30111482727402006 2209 12 0.16767390080198652 2209 13 0.3075078457530509 2209 14 0.36771157993675985 2209 15 -0.2703142418089399 2209 16 -0.2525545906441593 2209 17 0.1457767991017036 2209 18 -0.22961271031697095 2209 19 0.2333614474024941 2209 20 0.3469078571522104 2209 21 0.7384952632788355 2209 22 0.674347473530382 2209 23 0.4999553555495311 2209 24 0.6519364097005109 2209 25 0.8367273528477384 2210 1 -0.14308874564710194 2210 2 -0.19376608106795246 2210 3 -0.1021956868623235 2210 4 -0.1332930784957611 2210 5 -0.07195701555271228 2210 6 -0.6281087648422741 2210 7 -0.40413473585496007 2210 8 -0.4268623353756311 2210 9 -0.2149270208450318 2210 10 0.018661875094494462 2210 11 -0.07337914664045542 2210 12 -0.1734608743892494 2210 13 -0.025668644258509232 2210 14 -0.07472196802427788 2210 15 -0.3013835280342434 2210 16 -0.09720375689808236 2210 17 -1.1233630391020313 2210 18 -1.6417776984849297 2210 19 -0.4405618369516944 2210 20 -0.3432291743815483 2210 21 -0.6090915508755899 2210 22 -0.5569155279555034 2210 23 -0.26054874438993636 2210 24 0.033610505787684986 2210 25 0.025324017863418646 2211 1 -0.20018963964454528 2211 2 -0.2167418154229992 2211 3 -0.08602710448684547 2211 4 -0.0974948664906563 2211 5 -0.17700171542499987 2211 6 -0.09255320928671296 2211 7 -0.14594827353057127 2211 8 -0.1504766393347623 2211 9 -0.010891260947197168 2211 10 -0.17741730753641605 2211 11 -0.35563968303892324 2211 12 -0.08523609788861108 2211 13 -0.21513224579107515 2211 14 -0.2166376768365339 2211 15 -0.2952788026191726 2211 16 -0.22777846954176084 2211 17 -0.45156227281850647 2211 18 -0.3794533051260487 2211 19 -0.2251339953169591 2211 20 -0.28813338894093476 2211 21 0.1055316930579977 2211 22 -0.10181974251489123 2211 23 -0.7998718606095991 2211 24 -0.23592972409737456 2211 25 -1.0173579744737395 2212 1 -0.15243361815780077 2212 2 -0.2410333289884683 2212 3 -0.24608350200642093 2212 4 -0.1207991272580955 2212 5 -0.054175085961738034 2212 6 -0.2028568643139863 2212 7 -0.11944662292564345 2212 8 -0.4139712417307706 2212 9 -0.23461835500872752 2212 10 -0.5619490141093593 2212 11 -0.1845808818120389 2212 12 -0.34896174054338047 2212 13 -0.2850445837852007 2212 14 -0.4258760289270782 2212 15 -0.28866838536935546 2212 16 -0.5214935820924325 2212 17 -0.7114350229529123 2212 18 -0.4429015513675765 2212 19 -0.3965549077186381 2212 20 -0.7606564818591082 2212 21 -0.021320072400591528 2212 22 -0.31390818354112326 2212 23 -0.4518753202893748 2212 24 -0.5907484685712916 2212 25 -0.3924065151434917 2213 1 -0.04265424804830786 2213 2 -0.10894556605505556 2213 3 -0.11548199356148103 2213 4 0.10895294205344896 2213 5 -0.026571325900000886 2213 6 -0.23215391041107836 2213 7 -0.22800061019409412 2213 8 -0.16869879669266652 2213 9 -0.19928482227862787 2213 10 -0.20260456641435098 2213 11 0.19503733833239778 2213 12 0.34230558849533566 2213 13 0.27372642222005084 2213 14 -0.3232722651471036 2213 15 -0.05881473376864221 2213 16 -0.32164820134252636 2213 17 -0.16265849461910875 2213 18 -0.13363211542591813 2213 19 -0.1252314392203715 2213 20 -0.37849572989435387 2213 21 -0.11659087397706713 2213 22 -0.4180852086019087 2213 23 -0.48828149060286563 2213 24 -0.7590084502210388 2213 25 -0.5320501858901565 2214 1 -0.173975031293768 2214 2 -0.17509136801624336 2214 3 -0.093892527472903 2214 4 -0.1712992316137374 2214 5 -0.06980342426174246 2214 6 -0.005244015108509359 2214 7 -0.2353298606922607 2214 8 -0.15040747796231102 2214 9 -0.2606115552196613 2214 10 -0.008957341566258492 2214 11 0.10149128289450715 2214 12 -0.03160665595355994 2214 13 -0.20465337979276654 2214 14 -0.3529718792773835 2214 15 -0.13878620227398386 2214 16 -0.12995491060770092 2214 17 -0.497225303922761 2214 18 -0.32690963553379504 2214 19 -0.40344670264806126 2214 20 -0.4816885283041614 2214 21 -0.6056008397171482 2214 22 -0.6732414065497494 2214 23 -0.9026064890355414 2214 24 -0.49871111295566095 2214 25 -0.8070259998859584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 6.0 9 16.0 10 43.0 11 130.0 12 349.0 13 849.0 14 1768.0 15 3275.0 16 5067.0 17 6608.0 18 8025.0 19 8677.0 20 9097.0 21 9199.0 22 9466.0 23 9882.0 24 10877.0 25 11545.0 26 12814.0 27 14272.0 28 16227.0 29 19935.0 30 24806.0 31 32043.0 32 42124.0 33 56510.0 34 75785.0 35 103802.0 36 145801.0 37 226126.0 38 450665.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.24310668267234 21.26228762435966 13.188095553253094 23.306510139714913 2 15.626745625628423 22.737536185513026 37.88874963994987 23.74696854890868 3 18.49671834871112 27.46344525825169 30.035118424807383 24.004717968229812 4 12.104363450901582 19.16659129103633 36.386971138607805 32.34207411945428 5 12.434919242467204 39.03142652763514 33.432866001105296 15.100788228792359 6 29.13366255732093 39.19630227292248 16.809501839058267 14.860533330698326 7 24.911118594438538 36.01390175877621 21.131539919286485 17.94343972749877 8 24.96051045275773 35.48875061001658 20.33943527286935 19.211303664356333 9 25.16492068607005 17.00377873571954 21.82478279109641 36.00651778711399 10 16.19403879158053 30.51275183354586 31.055775965153664 22.237433409719948 11 30.942640872893644 26.866561562897612 22.50262569292816 19.688171871280584 12 21.964619848818103 28.80033379795496 29.791467479779836 19.443578873447102 13 27.501466801642817 24.149179047080533 26.914442144175197 21.43491200710145 14 21.37275958695003 24.76939411281429 28.870937464137054 24.98690883609863 15 23.349035094119365 29.788049029622066 26.388534218590397 20.474381657668175 16 20.076638485835105 30.902470610767086 27.494626793982007 21.526264109415802 17 19.105722878060234 29.3523505668462 29.166302499868902 22.37562405522466 18 19.322899012453394 27.07339947377461 32.486513764396335 21.117187749375656 19 19.984693513059153 27.742285576182073 33.4642820771222 18.808738833636575 20 21.114071741149395 25.941604485835846 33.41060020398454 19.533723569030222 21 21.340248338255613 26.46668987206126 32.70949835307565 19.483563436607472 22 20.984161004453767 26.40468481614909 32.57018863909283 20.040965540304313 23 20.84179150304494 26.40879350716566 32.83581182089226 19.91360316889714 24 20.726803461649247 26.252841451022924 32.6576677567604 20.36268733056744 25 20.67675858735404 26.639341770458714 32.73036665926924 19.953532982918013 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2552.0 1 2552.0 2 2533.5 3 2515.0 4 2515.0 5 2515.0 6 5179.0 7 7843.0 8 7843.0 9 7843.0 10 8704.0 11 9565.0 12 9565.0 13 9565.0 14 10797.5 15 12030.0 16 12030.0 17 12030.0 18 19727.5 19 27425.0 20 27425.0 21 27425.0 22 42096.0 23 56767.0 24 56767.0 25 56767.0 26 75724.0 27 94681.0 28 94681.0 29 94681.0 30 109724.5 31 124768.0 32 124768.0 33 124768.0 34 129753.0 35 134738.0 36 134738.0 37 134738.0 38 130392.0 39 126046.0 40 126046.0 41 126046.0 42 128695.5 43 131345.0 44 131345.0 45 131345.0 46 140448.5 47 149552.0 48 149552.0 49 149552.0 50 142693.0 51 135834.0 52 135834.0 53 135834.0 54 121759.5 55 107685.0 56 107685.0 57 107685.0 58 93134.5 59 78584.0 60 78584.0 61 78584.0 62 68516.5 63 58449.0 64 58449.0 65 58449.0 66 43825.0 67 29201.0 68 29201.0 69 29201.0 70 22480.0 71 15759.0 72 15759.0 73 15759.0 74 11301.5 75 6844.0 76 6844.0 77 6844.0 78 4630.5 79 2417.0 80 2417.0 81 2417.0 82 1618.5 83 820.0 84 820.0 85 820.0 86 530.5 87 241.0 88 241.0 89 241.0 90 164.0 91 87.0 92 87.0 93 87.0 94 58.5 95 30.0 96 30.0 97 30.0 98 20.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09538003433681236 2 0.0 3 0.1264640455270564 4 0.011856004268161536 5 0.023408008426883033 6 0.012920004651201675 7 0.02067200744192268 8 0.019076006867362473 9 3.8000013680004923E-4 10 0.4066001463760527 11 0.06824802456928884 12 0.0017480006292802267 13 7.600002736000985E-5 14 0.0012160004377601576 15 9.88000355680128E-4 16 7.600002736000985E-5 17 0.0 18 2.2800008208002955E-4 19 5.320001915200689E-4 20 2.2800008208002955E-4 21 2.2800008208002955E-4 22 0.0037240013406404826 23 0.0 24 0.0 25 0.0012920004651201676 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1315789.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.64017400468937 #Duplication Level Percentage of deduplicated Percentage of total 1 86.61314876000033 55.98689006940466 2 9.338185273434313 12.072438419256436 3 1.9988007549721478 3.8760848580631233 4 0.6975437149153477 1.8035738843202205 5 0.3365153702459693 1.0876206043975962 6 0.19625919228878208 0.7611737003739998 7 0.14463264654982347 0.6544355607817528 8 0.09703060622145909 0.5017660215948488 9 0.07607023802922028 0.4425474080508244 >10 0.4165786721925142 5.05591831004598 >50 0.04081133785796935 1.8396028446421897 >100 0.0366049232064614 4.873035966110561 >500 0.004856953235020951 2.2897223282224015 >1k 0.002369245480498025 2.902622385579505 >5k 2.3692454804980252E-4 1.1495321915348369 >10k+ 3.5538682207470374E-4 4.703035447621011 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 27332 2.077232747803789 No Hit GGTATCAACGCAGAGTACTTTTTTT 17255 1.31138047209697 No Hit TATCAACGCAGAGTACTTTTTTTTT 16831 1.279156460496326 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 9776 0.7429762674714563 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 5236 0.3979361432570116 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 4421 0.33599612095860354 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 3299 0.2507240902606725 No Hit ATCAACGCAGAGTACTTTTTTTTTT 3243 0.24646808872851195 No Hit GTACTGGTTCACTATCGGTCAGTCA 2876 0.21857607868738835 No Hit ATACAGGGTGACAGCCCCGTACACA 2042 0.15519205586914012 No Hit GTGGTATCAACGCAGAGTACTTTTT 2030 0.15428005554082 No Hit CCCATGTACTCTGCGTTGATACCAC 2019 0.15344405523985988 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1868 0.1419680511084984 No Hit GTTAATGATAGTGTGTCGAAACACA 1707 0.1297320467035368 No Hit GGATACCACGTGTCCCGCCCTACTC 1683 0.1279080460468966 No Hit GTACATGGGAAGCAGTGGTATCAAC 1656 0.1258560453081763 No Hit CAACGCAGAGTACTTTTTTTTTTTT 1482 0.11263204054753459 No Hit GGTATCAACGCAGAGTACATGGGGG 1458 0.11080803989089437 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAATTA 35 0.0021974966 16.255 15 GGTATCA 3620 0.0 13.263553 1 GTCTAAG 60 4.0632795E-4 12.67698 1 GAAATCG 155 1.8189894E-12 12.23541 16 CCAAGAC 140 5.2750693E-11 12.214457 3 AATCGCC 150 1.4551915E-11 12.011094 18 TCAGAAC 145 9.640644E-11 11.793269 3 ATCGCCG 145 1.0004442E-10 11.771308 19 ACCGGGG 205 0.0 11.563516 15 TAATACC 125 1.770968E-8 11.425391 4 CTAAGAC 125 1.8297214E-8 11.40016 3 ACTGATC 110 4.824542E-7 11.252278 8 CGGGGGA 205 0.0 11.101396 17 CTAGGAC 95 1.3594274E-5 11.0001545 3 CTAGACA 130 3.1579475E-8 10.985952 4 CAGTACT 70 0.0014637124 10.881324 4 ACGCTTC 115 8.561783E-7 10.763048 7 TGACTAT 125 2.1706364E-7 10.663698 6 CAAGACA 170 1.3278623E-10 10.6412945 4 GAACCGG 205 0.0 10.638434 13 >>END_MODULE