FastQCFastQC Report
Wed 25 May 2016
SRR1294594_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294594_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2919419
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC101780.3486310118554411No Hit
CCCATGTACTCTGCGTTGATACCAC91990.3150969422340541No Hit
GTATCAACGCAGAGTACTTTTTTTT89320.30595128688276674No Hit
GGTATCAACGCAGAGTACTTTTTTT54370.18623568593614004No Hit
TATCAACGCAGAGTACTTTTTTTTT48450.1659576785654954No Hit
GAGTACATGGGAAGCAGTGGTATCA46480.15920976057222344No Hit
GTATCAACGCAGAGTACATGGGAAG34850.11937306703833879No Hit
ATTCCATTCCATTCCATTCCATTCC33940.11625600847291877No Hit
CATGTACTCTGCGTTGATACCACTG32570.11156329392937431No Hit
GTGGTATCAACGCAGAGTACATGGG31180.10680207260417227No Hit
GCGTTGATACCACTGCTTCCCATGT29610.10142429024405199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGC456.783242E-414.77248610
TAGACGT550.00307622112.0874074
TCGGAAC600.00585670311.08867713
GCGTGCG600.005892690711.0795549
GTCTTAC3050.09.9662071
CCTACAC3650.09.3669893
CTAGTAC2357.2759576E-129.295023
GTCCTAC2151.07320375E-109.27813151
GCCTAGA3200.09.2021951
CCTAGTA3100.09.1907292
GTTCTAG4150.09.1557011
GGAACGA1356.8097634E-69.15255815
GTATTAC2700.09.1472231
TGTATCG1151.1148946E-49.0841695
CTGTCGC4400.09.0650899
ACAGCGT950.00183012588.9969318
TCTGGAC2750.08.9790643
GCACTGT4550.08.7663916
GTCGCCC3800.08.746716511
ACCGCGC1856.512164E-88.73528818
Produced by FastQC (version 0.11.5)