##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294594_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2919419 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.71867758619095 31.0 30.0 33.0 25.0 34.0 2 29.757285610595808 31.0 30.0 34.0 25.0 34.0 3 29.661231566965892 31.0 30.0 34.0 23.0 34.0 4 33.106005338733496 35.0 33.0 37.0 26.0 37.0 5 33.5966423456174 35.0 33.0 37.0 27.0 37.0 6 33.730826921384015 36.0 35.0 37.0 28.0 37.0 7 33.58271183410124 36.0 35.0 37.0 27.0 37.0 8 33.43781348275119 36.0 33.0 37.0 26.0 37.0 9 34.415949543385175 38.0 34.0 39.0 25.0 39.0 10 34.54422232642865 38.0 34.0 39.0 26.0 39.0 11 34.82010941218098 38.0 34.0 39.0 27.0 39.0 12 34.57293009328226 38.0 34.0 39.0 25.0 39.0 13 34.41668016821155 38.0 34.0 39.0 25.0 39.0 14 35.25347714733651 39.0 34.0 41.0 24.0 41.0 15 35.25185627688249 39.0 34.0 41.0 25.0 41.0 16 35.36975884585255 39.0 34.0 41.0 25.0 41.0 17 35.34653367673499 39.0 34.0 41.0 25.0 41.0 18 35.25837332702157 39.0 34.0 41.0 25.0 41.0 19 35.54246923788603 39.0 34.0 41.0 25.0 41.0 20 35.665352592416504 39.0 34.0 41.0 25.0 41.0 21 35.715280334888554 39.0 34.0 41.0 26.0 41.0 22 35.70467342988451 39.0 34.0 41.0 26.0 41.0 23 35.69723427846431 39.0 34.0 41.0 26.0 41.0 24 35.70296041780916 39.0 34.0 41.0 26.0 41.0 25 34.396679955840526 38.0 32.0 40.0 20.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.096410974306039 1101 2 1.0628326629995506 1101 3 1.4133659584446363 1101 4 1.212666988648671 1101 5 0.8251032321266578 1101 6 0.8061338180222037 1101 7 0.8704188617191875 1101 8 1.074736647405544 1101 9 1.4071237935024783 1101 10 1.2946084711222028 1101 11 1.0407226398194638 1101 12 1.203123095433277 1101 13 1.3162125693225235 1101 14 1.32448338047017 1101 15 1.5016348792483214 1101 16 1.5086977692696166 1101 17 1.436792598310447 1101 18 1.404282274505512 1101 19 1.3439978920610542 1101 20 1.1801228975578084 1101 21 1.086383078080111 1101 22 1.127849012497478 1101 23 1.1339729091917476 1101 24 1.1725535031778804 1101 25 1.0980126448208836 1102 1 1.0354735062465927 1102 2 1.0060116936420407 1102 3 1.3007376000017672 1102 4 1.1472825436136773 1102 5 0.9243284820295585 1102 6 0.790493167150359 1102 7 0.7874236723926131 1102 8 0.8373195224983832 1102 9 1.1851940048041527 1102 10 1.1185299876427095 1102 11 0.907078248069837 1102 12 1.1033109910702876 1102 13 1.1269498765719206 1102 14 1.234209151642922 1102 15 1.3420930856711806 1102 16 1.1581838244492673 1102 17 1.2779577556794308 1102 18 1.317922680073906 1102 19 1.0504914494382191 1102 20 0.39706059047875897 1102 21 0.6771129921557559 1102 22 0.6551509469878241 1102 23 0.9009831710531913 1102 24 0.9974213118684361 1102 25 0.9216309390078692 1103 1 0.7330509499410844 1103 2 0.729529779316298 1103 3 1.0064255837654557 1103 4 0.838913560037696 1103 5 0.5386825070547516 1103 6 0.512464146553981 1103 7 0.44797320469619706 1103 8 0.4991270918539996 1103 9 0.5288136123692482 1103 10 0.7116926021644119 1103 11 0.5888686060879209 1103 12 0.7293702686199026 1103 13 0.684750048099346 1103 14 0.46790911459128637 1103 15 0.7125667353517073 1103 16 0.7808329641460645 1103 17 0.8712481075450356 1103 18 0.9130459846975825 1103 19 0.6955021440227185 1103 20 -0.1517818533202302 1103 21 0.2384207647387555 1103 22 -0.34988715839001117 1103 23 0.17693860829870545 1103 24 0.4127812587109503 1103 25 0.42625614416400737 1104 1 0.2705901874054071 1104 2 0.3111134331646852 1104 3 0.6381316407068773 1104 4 0.4081665723375778 1104 5 0.3372273841912943 1104 6 0.20691629599716777 1104 7 0.11957845317370186 1104 8 0.30839955169967226 1104 9 0.555249043963947 1104 10 0.46056112793444015 1104 11 0.2875050534271253 1104 12 0.5470708042688344 1104 13 0.22972264280505783 1104 14 0.6367488810505151 1104 15 0.5048441601825147 1104 16 0.4545015403628838 1104 17 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0.14355600307983707 2207 12 0.4409496928676262 2207 13 0.6584853660392085 2207 14 0.8685370179945124 2207 15 0.9342257491529935 2207 16 0.8985597692358809 2207 17 0.8908957196181362 2207 18 0.8894799219424883 2207 19 -0.3387479866877996 2207 20 0.25813909685007275 2207 21 -0.3089638788358613 2207 22 0.3366622945480984 2207 23 -0.5067484598066798 2207 24 -1.552694312561492 2207 25 -0.1341147114375545 2208 1 0.4240897262729604 2208 2 0.10186857729800991 2208 3 0.5627847049981369 2208 4 0.39162937840680456 2208 5 0.3504598765132343 2208 6 0.24310970045583957 2208 7 0.2890966699081332 2208 8 0.44056892313451357 2208 9 -0.22613060022484888 2208 10 0.058306428182767434 2208 11 0.15415757295333776 2208 12 0.2112269452445119 2208 13 0.4120099434918174 2208 14 0.666142736469773 2208 15 0.5254396898560643 2208 16 0.3044090286245762 2208 17 0.24436727240288292 2208 18 0.5842817240504559 2208 19 -1.401600776453428 2208 20 -0.1639309379317453 2208 21 -0.27963708507624574 2208 22 0.07476439536421253 2208 23 0.2739550150924046 2208 24 0.4366538650497418 2208 25 0.39894648504902364 2209 1 0.23719693734320657 2209 2 -1.2666485676750234 2209 3 -0.1597859492508249 2209 4 0.2853229428513586 2209 5 0.20006108100972853 2209 6 0.21773799586343756 2209 7 0.2570207717810291 2209 8 0.4383782771304112 2209 9 -1.8343695688058475 2209 10 -0.23723016552826692 2209 11 0.13303481838638476 2209 12 0.45909647564720046 2209 13 0.5613955100999561 2209 14 0.8285402406014271 2209 15 0.8453589969835349 2209 16 0.7876028360803673 2209 17 0.6232089941582402 2209 18 0.796072962411742 2209 19 0.4954079085468308 2209 20 0.46436957082404007 2209 21 0.5551318455913119 2209 22 0.3687071500787269 2209 23 0.41828169989346264 2209 24 0.4946580418918316 2209 25 0.6187098292248692 2210 1 0.085916972311189 2210 2 0.13503294985109804 2210 3 0.051808516960836215 2210 4 0.2239591389248332 2210 5 0.17174307249944576 2210 6 0.10967297859009761 2210 7 0.03144174625018792 2210 8 0.19054666059855663 2210 9 0.33864031070207545 2210 10 -1.4141607772572868 2210 11 -0.4646424966624849 2210 12 -2.2999522794084086 2210 13 -0.7742681442647239 2210 14 -0.5268824296041714 2210 15 -0.7100196999325377 2210 16 -0.07964390936957955 2210 17 -0.020628425794569694 2210 18 0.3773353381279563 2210 19 0.338403516484135 2210 20 0.21120323066563174 2210 21 0.3473633188808307 2210 22 0.25278486957653 2210 23 0.19772932164944024 2210 24 0.17876130276140145 2210 25 0.06923197912375656 2211 1 -0.485729574683063 2211 2 -0.20697470026565767 2211 3 -1.5362451300176332 2211 4 -0.6482158060342087 2211 5 -0.43668360838048415 2211 6 -0.5213134498732828 2211 7 -0.5455733533794103 2211 8 -0.1400233196312044 2211 9 0.07199889975176887 2211 10 0.010217566600452699 2211 11 -0.33930199282673357 2211 12 -0.27682086728498945 2211 13 -0.3346613303093875 2211 14 -0.9946182668460608 2211 15 -0.465734721793126 2211 16 -0.17486788002933906 2211 17 -0.19108142421529806 2211 18 -0.10087551451373145 2211 19 -0.18174698828165248 2211 20 -0.059130465923431075 2211 21 -0.19672411177393911 2211 22 -0.25698579791375664 2211 23 -0.63512249080717 2211 24 -0.39736600392289034 2211 25 -0.24587901823037583 2212 1 -0.3158726805127152 2212 2 -0.26111205285076977 2212 3 -2.2576340093947174 2212 4 -1.2595818756136943 2212 5 -0.6008549216862988 2212 6 -0.3719872688831174 2212 7 -0.23042719668247003 2212 8 -0.26500153900378365 2212 9 -0.8265730934507758 2212 10 -0.1605813752432823 2212 11 -0.1306930821714687 2212 12 0.006665512731139245 2212 13 -0.2854911927467967 2212 14 0.013582399985459404 2212 15 0.14175167945082734 2212 16 -1.114304120946393 2212 17 -1.4022318292559959 2212 18 -0.3343757810618584 2212 19 -0.15180164233579063 2212 20 -0.2521111209356022 2212 21 -0.046989453201305764 2212 22 -0.2948111189876883 2212 23 -0.06720761992349367 2212 24 -0.08311053115723865 2212 25 0.00895618628891981 2213 1 -3.10368997943403 2213 2 -1.4485677771133822 2213 3 -2.4680463460283875 2213 4 -1.2413017653855647 2213 5 -0.9699520331591387 2213 6 -0.7186201713790012 2213 7 -0.4385862091941988 2213 8 -0.3136675407972902 2213 9 -0.4776167705097052 2213 10 -0.2610088564070594 2213 11 -0.5958399798571747 2213 12 -0.3591654027002349 2213 13 -0.8167146489185058 2213 14 -0.36649642446062813 2213 15 -0.38942994504409967 2213 16 -1.907893659223106 2213 17 -1.2096777613149712 2213 18 -0.5028074030960994 2213 19 -0.3969590105766514 2213 20 -0.22541466355323792 2213 21 -0.16743009970488032 2213 22 -0.05741149838570436 2213 23 -0.05749735050621041 2213 24 -0.0170364998631527 2213 25 -0.07412198933388936 2214 1 -0.7712295885765279 2214 2 -0.5732928216698205 2214 3 -6.307986838790029 2214 4 -1.9921833861155704 2214 5 -1.1668230350252031 2214 6 -0.7096429150432897 2214 7 -0.3157318643207887 2214 8 -0.2459470782207589 2214 9 0.010660740558705584 2214 10 0.08383426219376844 2214 11 -0.6379073847908714 2214 12 -0.3316600391242588 2214 13 -4.937655144861026 2214 14 -3.3790295522265197 2214 15 -1.7329039048187695 2214 16 -2.480389720125501 2214 17 -3.121338804058034 2214 18 -0.9631838775176504 2214 19 -0.5699386749723132 2214 20 -0.07052829474638145 2214 21 -0.11378624944770621 2214 22 -0.021907688735844033 2214 23 -0.07087921844544809 2214 24 0.08047070447079818 2214 25 0.29459559427503734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1074.0 3 144.0 4 165.0 5 298.0 6 51.0 7 115.0 8 256.0 9 558.0 10 1311.0 11 2956.0 12 5809.0 13 9793.0 14 14801.0 15 19719.0 16 23448.0 17 25973.0 18 26798.0 19 27266.0 20 26983.0 21 27066.0 22 27173.0 23 27944.0 24 29161.0 25 31144.0 26 33857.0 27 37807.0 28 43379.0 29 52307.0 30 63487.0 31 78189.0 32 100661.0 33 130655.0 34 172560.0 35 230716.0 36 318500.0 37 471195.0 38 856100.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.79488164138574 20.745432975834543 13.192219601173985 22.26746578160573 2 15.237411768253981 21.277473676129688 41.247904303255375 22.23721025236096 3 19.211106149241537 27.73977168469333 28.25570711153959 24.79341505452554 4 12.134763612261942 17.330177224403936 38.04901336182833 32.48604580150579 5 12.831105685830076 39.21692267306734 33.88828612219357 14.063685518909017 6 30.61788577200674 38.189897380651324 17.771872378946085 13.420344468395854 7 26.061676678313216 33.81180846462266 22.439044119108775 17.687470737955348 8 24.581679765539825 36.99708775565252 20.856426730680976 17.56480574812668 9 25.01023122920764 17.828628110413643 21.788165226982475 35.37297543339624 10 15.069440183825659 31.062224533143766 32.39077211095485 21.47756317207572 11 31.605473363553543 27.235241255594318 22.07592708488279 19.08335829596935 12 21.152005264429626 29.058212047523817 30.785397377039065 19.00438531100749 13 26.407147927258066 24.92534249228062 26.405639867880286 22.26186971258102 14 20.551431361386232 24.178890440007855 28.178640920452956 27.091037278152953 15 23.416916907824763 30.557915818200325 24.251050293074357 21.774116980900555 16 21.22306806197666 32.08159636478173 25.735490436062364 20.959845137179244 17 19.371763997798162 30.699665406026806 28.51229415204318 21.416276444131853 18 19.710321407745344 30.232223098823447 29.71306555071519 20.344389942716017 19 20.378911533380727 29.086891277418523 30.316795899998013 20.217401289202737 20 22.54505521924897 28.32475677276376 29.077213722588308 20.05297428539896 21 22.833251248157367 28.991250302892585 27.993032870743097 20.18246557820695 22 21.02120083934128 28.652495299099307 29.64840758916973 20.677896272389688 23 20.781247911356157 29.13335547510028 29.179935446124045 20.90546116741952 24 20.75056405939026 28.4951971825482 29.481504130950903 21.272734627110637 25 21.3890311360095 28.454051437929866 28.394581858590595 21.762335567470036 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3000.0 1 3000.0 2 3122.0 3 3244.0 4 3244.0 5 3244.0 6 5106.0 7 6968.0 8 6968.0 9 6968.0 10 10253.0 11 13538.0 12 13538.0 13 13538.0 14 20805.5 15 28073.0 16 28073.0 17 28073.0 18 43766.0 19 59459.0 20 59459.0 21 59459.0 22 84956.5 23 110454.0 24 110454.0 25 110454.0 26 143399.5 27 176345.0 28 176345.0 29 176345.0 30 210459.0 31 244573.0 32 244573.0 33 244573.0 34 273334.5 35 302096.0 36 302096.0 37 302096.0 38 319515.0 39 336934.0 40 336934.0 41 336934.0 42 337130.5 43 337327.0 44 337327.0 45 337327.0 46 348310.0 47 359293.0 48 359293.0 49 359293.0 50 337953.0 51 316613.0 52 316613.0 53 316613.0 54 280214.5 55 243816.0 56 243816.0 57 243816.0 58 209250.0 59 174684.0 60 174684.0 61 174684.0 62 142668.5 63 110653.0 64 110653.0 65 110653.0 66 83178.0 67 55703.0 68 55703.0 69 55703.0 70 39631.0 71 23559.0 72 23559.0 73 23559.0 74 16199.0 75 8839.0 76 8839.0 77 8839.0 78 5873.5 79 2908.0 80 2908.0 81 2908.0 82 1915.5 83 923.0 84 923.0 85 923.0 86 593.0 87 263.0 88 263.0 89 263.0 90 181.0 91 99.0 92 99.0 93 99.0 94 65.5 95 32.0 96 32.0 97 32.0 98 27.5 99 23.0 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09793044437951524 2 0.05257895492219514 3 0.055216466016012086 4 0.050695018426611595 5 0.04771497342450673 6 0.06638307142619816 7 0.06223841113591437 8 0.05953239326043984 9 0.06443062814895703 10 0.0641223476314979 11 0.059087099179665536 12 0.05905284578883675 13 0.060354474640330835 14 0.06206714418177041 15 0.06508144257470408 16 0.059600900042097414 17 0.06347153320575087 18 0.14108971682379268 19 0.0670338858519452 20 0.060868275502762706 21 0.058847325443864 22 0.06484166883890254 23 0.06357429337823724 24 0.07443261827096419 25 0.06717089941526037 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2919419.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.6470238942879 #Duplication Level Percentage of deduplicated Percentage of total 1 92.44957293485997 75.48232490429224 2 5.800460833338365 9.471807285149172 3 0.8566377840605066 2.098257768718141 4 0.2889001988548998 0.9435136575588212 5 0.14292296865034562 0.5834617518218665 6 0.08576907064107711 0.42016736160137325 7 0.06260551815995746 0.3578087965984225 8 0.042119892143153245 0.27511710721894894 9 0.036230560458053715 0.26623056918799387 >10 0.20213802479685286 3.127489323603847 >50 0.017174120155275717 0.9798061441094806 >100 0.012760286770544376 2.060094932362343 >500 0.0015654509863079155 0.9215073810263125 >1k 9.731181803427087E-4 1.8466764718301036 >5k 1.269284583055707E-4 0.8141432730010828 >10k+ 4.23094861018569E-5 0.35159327191976497 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 10178 0.3486310118554411 No Hit CCCATGTACTCTGCGTTGATACCAC 9199 0.3150969422340541 No Hit GTATCAACGCAGAGTACTTTTTTTT 8932 0.30595128688276674 No Hit GGTATCAACGCAGAGTACTTTTTTT 5437 0.18623568593614004 No Hit TATCAACGCAGAGTACTTTTTTTTT 4845 0.1659576785654954 No Hit GAGTACATGGGAAGCAGTGGTATCA 4648 0.15920976057222344 No Hit GTATCAACGCAGAGTACATGGGAAG 3485 0.11937306703833879 No Hit ATTCCATTCCATTCCATTCCATTCC 3394 0.11625600847291877 No Hit CATGTACTCTGCGTTGATACCACTG 3257 0.11156329392937431 No Hit GTGGTATCAACGCAGAGTACATGGG 3118 0.10680207260417227 No Hit GCGTTGATACCACTGCTTCCCATGT 2961 0.10142429024405199 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.425339082879162E-5 0.0 8 0.0 0.0 0.0 3.425339082879162E-5 0.0 9 0.0 0.0 0.0 3.425339082879162E-5 0.0 10 0.0 0.0 0.0 3.425339082879162E-5 0.0 11 0.0 0.0 0.0 3.425339082879162E-5 0.0 12 0.0 0.0 0.0 3.425339082879162E-5 0.0 13 0.0 0.0 0.0 3.425339082879162E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGCGC 45 6.783242E-4 14.772486 10 TAGACGT 55 0.003076221 12.087407 4 TCGGAAC 60 0.005856703 11.088677 13 GCGTGCG 60 0.0058926907 11.079554 9 GTCTTAC 305 0.0 9.966207 1 CCTACAC 365 0.0 9.366989 3 CTAGTAC 235 7.2759576E-12 9.29502 3 GTCCTAC 215 1.07320375E-10 9.2781315 1 GCCTAGA 320 0.0 9.202195 1 CCTAGTA 310 0.0 9.190729 2 GTTCTAG 415 0.0 9.155701 1 GGAACGA 135 6.8097634E-6 9.152558 15 GTATTAC 270 0.0 9.147223 1 TGTATCG 115 1.1148946E-4 9.084169 5 CTGTCGC 440 0.0 9.065089 9 ACAGCGT 95 0.0018301258 8.996931 8 TCTGGAC 275 0.0 8.979064 3 GCACTGT 455 0.0 8.766391 6 GTCGCCC 380 0.0 8.7467165 11 ACCGCGC 185 6.512164E-8 8.735288 18 >>END_MODULE