FastQCFastQC Report
Wed 25 May 2016
SRR1294594_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294594_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2919419
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT240310.8231432350066913No Hit
GGTATCAACGCAGAGTACTTTTTTT166810.571380812415073No Hit
TATCAACGCAGAGTACTTTTTTTTT144490.49492724408521005No Hit
GTACATGGGAAGCAGTGGTATCAAC103760.35541318323954185No Hit
CCCATGTACTCTGCGTTGATACCAC103620.35493363576793874No Hit
ACGCAGAGTACTTTTTTTTTTTTTT91510.3134527794742721No Hit
GAGTACATGGGAAGCAGTGGTATCA50520.17304813046705525No Hit
CATGTACTCTGCGTTGATACCACTG38480.13180704790919015No Hit
GAGTACTTTTTTTTTTTTTTTTTTT37070.12697731980233054No Hit
GTATCAACGCAGAGTACATGGGAAG36180.12392876801856807No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34290.11745487715192646No Hit
GTGGTATCAACGCAGAGTACATGGG33960.11632451525457634No Hit
ATTCCATTCCATTCCATTCCATTCC32710.11204284140097738No Hit
GGTATCAACGCAGAGTACATGGGAA32610.11170030749268947No Hit
GCGTTGATACCACTGCTTCCCATGT32440.11111799984860002No Hit
ACGCAGAGTACATGGGAAGCAGTGG31290.10717885990328899No Hit
GTGGTATCAACGCAGAGTACTTTTT29270.10025967495587307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG550.003020770112.1167997
TCGCGCG550.003023494512.115349
CGCGCGC700.001467164410.87908110
TAACCCG1158.5849024E-710.7621255
TATTCCG1158.5849024E-710.7621255
GTCTAGC1605.366019E-1010.6975081
CCCGTAC1054.1899708E-59.93440216
TGTATCG1252.5025201E-69.90115455
GGACTGT3150.09.6714586
TTATACT4900.09.5203424
GTCCTAG2800.09.5088961
GTATACG1002.730903E-49.5088961
TCGCTCT2054.0745363E-109.2881384
ATTACAC3400.09.2205133
CAGGACT3850.09.1494194
CCGTACA1252.8149472E-59.10352517
CCCCGTA1151.1330619E-49.07069615
CGCTCTG2106.4210326E-109.0669925
CTAGGAC3250.09.0614653
AGCACCG950.00178928779.0192715
Produced by FastQC (version 0.11.5)