##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294594_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2919419 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.123194375319198 31.0 31.0 34.0 28.0 34.0 2 31.325680212398424 31.0 31.0 34.0 28.0 34.0 3 31.400223469121766 31.0 31.0 34.0 28.0 34.0 4 35.00424228245414 37.0 35.0 37.0 32.0 37.0 5 35.2112259322831 37.0 35.0 37.0 32.0 37.0 6 34.72388067625784 37.0 35.0 37.0 32.0 37.0 7 34.574115260604934 37.0 35.0 37.0 31.0 37.0 8 34.58267175763397 37.0 35.0 37.0 31.0 37.0 9 36.01089634615655 39.0 35.0 39.0 30.0 39.0 10 35.78446670382018 39.0 35.0 39.0 30.0 39.0 11 35.95619539367251 39.0 35.0 39.0 30.0 39.0 12 35.91200749190164 39.0 35.0 39.0 30.0 39.0 13 35.75726129068832 39.0 35.0 39.0 30.0 39.0 14 36.80645361285927 40.0 36.0 41.0 30.0 41.0 15 36.8505079264059 40.0 36.0 41.0 30.0 41.0 16 36.889857536722204 40.0 36.0 41.0 30.0 41.0 17 36.94136299037582 40.0 36.0 41.0 30.0 41.0 18 36.966632059324134 40.0 36.0 41.0 30.0 41.0 19 36.997143267204876 40.0 36.0 41.0 30.0 41.0 20 37.06583056423213 40.0 36.0 41.0 30.0 41.0 21 36.91735410367611 40.0 36.0 41.0 30.0 41.0 22 36.897255584073406 40.0 36.0 41.0 30.0 41.0 23 36.91171085753707 40.0 36.0 41.0 30.0 41.0 24 36.84250462163876 40.0 36.0 41.0 29.0 41.0 25 35.74859449774082 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5246315261993395 1101 2 0.5436821491175721 1101 3 0.5066903064813282 1101 4 0.4009807925179274 1101 5 0.41514339015453317 1101 6 0.5211196532823621 1101 7 0.5361339357645889 1101 8 0.5014596884815106 1101 9 0.7479154627993339 1101 10 0.784831202062847 1101 11 0.7673085853465338 1101 12 0.887009620694343 1101 13 0.773683773958318 1101 14 1.0413909863043855 1101 15 1.0255093543624838 1101 16 0.9362563669178314 1101 17 1.0791493587892163 1101 18 0.9776941530510399 1101 19 1.1570002903286039 1101 20 0.9917425655709806 1101 21 1.3115802198794313 1101 22 1.2184354601135183 1101 23 1.1235828661556937 1101 24 1.3092988787724522 1101 25 1.2617804212596724 1102 1 0.39692722589338914 1102 2 0.40320673738252566 1102 3 0.3782986454361996 1102 4 0.35519507190494437 1102 5 0.37797906409171844 1102 6 0.47576491722207237 1102 7 0.5342422031726173 1102 8 0.6058729362311936 1102 9 0.7882738443416102 1102 10 0.8690554639379258 1102 11 0.7165776151032048 1102 12 0.8285166697618536 1102 13 0.8372548472290973 1102 14 1.0779105390344057 1102 15 1.0066192843828574 1102 16 1.0268005999016054 1102 17 0.951918630442357 1102 18 0.9698418870603192 1102 19 1.026675161033232 1102 20 1.054605836147509 1102 21 1.3977319877678482 1102 22 1.265704925965231 1102 23 1.1800018103968597 1102 24 1.1620086302664205 1102 25 1.2378369296374672 1103 1 0.3270755047303382 1103 2 0.3297733900058972 1103 3 0.23951778314662775 1103 4 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0.2039354098965731 2207 5 0.15338243512147898 2207 6 0.35472460344639956 2207 7 0.4101284863976744 2207 8 0.375689356529449 2207 9 0.45641488294224786 2207 10 0.6316524542962156 2207 11 0.5450416516843717 2207 12 0.5425675682085966 2207 13 0.612156125836826 2207 14 0.605572832263789 2207 15 0.562981812391051 2207 16 0.6218441158124293 2207 17 0.6213881527658458 2207 18 0.6557319791772471 2207 19 0.7207799813426519 2207 20 0.6571331580966273 2207 21 1.051727485721834 2207 22 0.9288081311366341 2207 23 0.82213667084158 2207 24 0.9676947282060411 2207 25 0.9303376584613972 2208 1 0.18799833590222192 2208 2 0.1770279159211654 2208 3 0.1684038114958888 2208 4 0.15903883264671492 2208 5 0.04423455191516723 2208 6 0.23001485304336455 2208 7 0.2705155740124212 2208 8 0.1862072746683623 2208 9 0.33470877148246103 2208 10 0.4692373398706877 2208 11 0.06698466040249684 2208 12 -0.21482462608241093 2208 13 0.028109272514207362 2208 14 -0.3772644404066554 2208 15 0.17155203814981945 2208 16 0.20685342224188474 2208 17 0.21792523541044062 2208 18 0.37050629985777306 2208 19 0.4910396612877719 2208 20 0.339385257190294 2208 21 0.6796331569081175 2208 22 0.6382133779747861 2208 23 0.6812309958276401 2208 24 0.7871266538232717 2208 25 0.7396665667123088 2209 1 0.004818529259853932 2209 2 -0.0034427996108945536 2209 3 0.02705496540730934 2209 4 -0.5052238518942644 2209 5 -0.05389944918414358 2209 6 0.10310111073487604 2209 7 0.08626763614952182 2209 8 0.1953380645148286 2209 9 0.28112364100341125 2209 10 0.33761894382785584 2209 11 0.29591637585893693 2209 12 0.3151217535112849 2209 13 0.30575214652095184 2209 14 0.37645465174578874 2209 15 -0.14365195179956913 2209 16 -0.23551954098272176 2209 17 0.10322035803197593 2209 18 -0.2887191467848922 2209 19 0.18873071446250833 2209 20 0.22245584134873297 2209 21 0.4739625911484424 2209 22 0.45922926177944845 2209 23 0.41824012581052017 2209 24 0.5125890322715421 2209 25 0.5519844523854402 2210 1 -0.1712510485965666 2210 2 -0.14477611187917816 2210 3 -0.07552129006960229 2210 4 -0.042972419470942214 2210 5 -0.04840673769105308 2210 6 -0.5293045705088062 2210 7 -0.11405044608842729 2210 8 -0.23051822972177405 2210 9 -0.10145977659011862 2210 10 0.1331312984790145 2210 11 0.18316438521181766 2210 12 0.1302614674479159 2210 13 0.15991881318761614 2210 14 0.044952661098669466 2210 15 -0.010053462163568838 2210 16 0.08863206417400704 2210 17 -1.022058753865565 2210 18 -1.4542562002058546 2210 19 -0.269061510427953 2210 20 -0.13288651835804188 2210 21 -0.44060547002445816 2210 22 -0.2563873122010065 2210 23 -0.14023513328957904 2210 24 0.2593739791876146 2210 25 0.1449905928471864 2211 1 -0.23257078399959497 2211 2 -0.198306404052488 2211 3 -0.10396081150624781 2211 4 -0.04747239352410304 2211 5 -0.11839113801137557 2211 6 -0.01139490732854398 2211 7 -0.24657965385670622 2211 8 -0.15433176067289622 2211 9 -0.04228195114482958 2211 10 -0.015178829563716079 2211 11 -0.41496032869525123 2211 12 -0.09392907016042784 2211 13 -0.08121888206233763 2211 14 -0.08265841445345501 2211 15 -0.09400843227584943 2211 16 -0.1633892421417329 2211 17 -0.3908506899578228 2211 18 -0.3065516969728108 2211 19 -0.1733350469354562 2211 20 -0.31525057495158393 2211 21 -0.019862573038288645 2211 22 -0.18782870699337195 2211 23 -0.7758203482750687 2211 24 -0.25776264101127566 2211 25 -0.8173445784516957 2212 1 -0.24527980378859837 2212 2 -0.30548234843458744 2212 3 -0.2142557922767523 2212 4 -0.05025601772700128 2212 5 -0.10417361629108512 2212 6 -0.17025784422519052 2212 7 -0.27998485009842256 2212 8 -0.5033087756194305 2212 9 -0.2890312833214139 2212 10 -0.6297200376262921 2212 11 -0.23822862192675132 2212 12 -0.36020303690578714 2212 13 -0.35047940085761553 2212 14 -0.40089881016022844 2212 15 -0.29026227808076044 2212 16 -0.521113919343037 2212 17 -0.6179056597757793 2212 18 -0.407559127594169 2212 19 -0.48106678744102993 2212 20 -0.6135696727995708 2212 21 -0.1322868746967245 2212 22 -0.35978402799275244 2212 23 -0.3505632168332937 2212 24 -0.5474273905615377 2212 25 -0.2857969743712516 2213 1 -0.28349560097266746 2213 2 -0.26641854535125375 2213 3 -0.14733794490158658 2213 4 -0.12654874954964157 2213 5 -0.16339277251224615 2213 6 -0.3672740020262424 2213 7 -0.3882903200324179 2213 8 -0.4193928358751293 2213 9 -0.4727456329565598 2213 10 -0.37333694938180173 2213 11 -0.17365474505739087 2213 12 -0.01589262378439571 2213 13 -0.0784376319615987 2213 14 -0.6982518027411899 2213 15 -0.3470206144223411 2213 16 -0.4987718801438987 2213 17 -0.4768802370231242 2213 18 -0.32977595108418 2213 19 -0.4655828155572337 2213 20 -0.434017342236487 2213 21 -0.23982806859007155 2213 22 -0.4795219821324608 2213 23 -0.6229162293657708 2213 24 -0.8632578138555473 2213 25 -0.6049377530602698 2214 1 -0.2031402918103815 2214 2 -0.06022640700591353 2214 3 -0.0529222735373196 2214 4 -0.07768762001020946 2214 5 -0.04303569035842969 2214 6 -0.11361229738478329 2214 7 -0.0414613679381759 2214 8 -0.0886931970979532 2214 9 -0.0912750955257593 2214 10 -0.14422301780351177 2214 11 0.08331389304585457 2214 12 -0.24497294873695097 2214 13 -0.07010719944709365 2214 14 -0.1671237693801828 2214 15 0.01530237512351107 2214 16 -0.17247083696933885 2214 17 -0.21924921647564588 2214 18 -0.23144199609741634 2214 19 -0.4238798972989173 2214 20 -0.355467386540532 2214 21 -0.535417634604066 2214 22 -0.7674232372915313 2214 23 -0.9730801895926717 2214 24 -0.48511453982062136 2214 25 -0.7491829226610136 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 11.0 9 28.0 10 96.0 11 220.0 12 619.0 13 1597.0 14 3252.0 15 5560.0 16 8193.0 17 10608.0 18 12238.0 19 13796.0 20 14641.0 21 15621.0 22 16661.0 23 18325.0 24 20034.0 25 22354.0 26 25214.0 27 28128.0 28 33322.0 29 41099.0 30 52103.0 31 66789.0 32 88808.0 33 119250.0 34 162357.0 35 225203.0 36 320864.0 37 507700.0 38 1084727.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.64187919932499 20.94498247841988 12.539698267453266 23.87344005480187 2 14.990311428404077 21.74627896852079 39.81059245007311 23.452817153002016 3 18.90859235436327 27.173986120296256 28.8163354069651 25.101086118375378 4 11.781719645505962 17.484353000421365 38.034250399953415 32.69967695411926 5 12.918046378887105 38.41656716750292 34.20258772057551 14.462798733034465 6 30.729076737130313 38.32848227720086 17.164516579923205 13.777924405745622 7 26.00215370668132 33.784928781380955 22.311592636282935 17.90132487565479 8 24.72968782922856 36.72702916140615 20.533549407195874 18.009733602169415 9 24.912413869642243 16.75087312102574 22.076329069004306 36.26038394032771 10 15.116496203569426 30.501436965909328 32.49984610691769 21.882220723603556 11 31.867985726296837 26.371595454104547 21.97808902247207 19.782329797126543 12 21.436863326474555 28.556885331199545 30.988640369010746 19.01761097331516 13 26.949730031715234 23.923303865121014 26.730919221200672 22.396046881963077 14 20.800084401135578 23.337462046891954 28.727762493012243 27.134691058960218 15 23.643727636380827 29.64383998969638 24.9028041058286 21.809628268094194 16 21.138077710115795 31.400650952554177 26.266846702797203 21.194424634532822 17 19.21461743402281 30.160429236238183 29.069184337426158 21.555768992312853 18 19.407495299552682 29.31404126117566 30.78009442341465 20.49836901585701 19 20.018126987563214 28.22230153825918 31.71876862537696 20.040802848800645 20 22.10533418875644 27.366220538169834 30.59560260915465 19.932842663919082 21 22.35354242566654 28.217874010245897 29.35803511117992 20.07054845290764 22 20.555490091568892 27.965815467374043 30.927965712704143 20.550728728352922 23 20.334519646092875 28.357065297440236 30.594811965431372 20.71360309103552 24 20.2210578344923 27.86220673511184 30.892161003378416 21.02457442701745 25 20.943771730008393 27.750286878928527 29.75292445236097 21.553016938702108 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1807.0 1 1807.0 2 1993.5 3 2180.0 4 2180.0 5 2180.0 6 4557.5 7 6935.0 8 6935.0 9 6935.0 10 9847.5 11 12760.0 12 12760.0 13 12760.0 14 17981.0 15 23202.0 16 23202.0 17 23202.0 18 38304.0 19 53406.0 20 53406.0 21 53406.0 22 81076.5 23 108747.0 24 108747.0 25 108747.0 26 144013.5 27 179280.0 28 179280.0 29 179280.0 30 215584.0 31 251888.0 32 251888.0 33 251888.0 34 278604.0 35 305320.0 36 305320.0 37 305320.0 38 320223.5 39 335127.0 40 335127.0 41 335127.0 42 334419.5 43 333712.0 44 333712.0 45 333712.0 46 347745.0 47 361778.0 48 361778.0 49 361778.0 50 340157.0 51 318536.0 52 318536.0 53 318536.0 54 282178.5 55 245821.0 56 245821.0 57 245821.0 58 211320.0 59 176819.0 60 176819.0 61 176819.0 62 144383.0 63 111947.0 64 111947.0 65 111947.0 66 83729.5 67 55512.0 68 55512.0 69 55512.0 70 39265.0 71 23018.0 72 23018.0 73 23018.0 74 15659.0 75 8300.0 76 8300.0 77 8300.0 78 5380.0 79 2460.0 80 2460.0 81 2460.0 82 1566.0 83 672.0 84 672.0 85 672.0 86 408.5 87 145.0 88 145.0 89 145.0 90 91.5 91 38.0 92 38.0 93 38.0 94 22.5 95 7.0 96 7.0 97 7.0 98 4.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09351175696260113 2 0.0 3 0.13985659475395618 4 0.011269365582672443 5 0.021716649785453887 6 0.015996333517045686 7 0.02500497530501788 8 0.022744251510317635 9 2.3977373580154132E-4 10 0.39583218441751594 11 0.06460189510310099 12 0.0015756559781244144 13 6.850678165758325E-5 14 9.248415523773737E-4 15 0.001952443277241122 16 1.712669541439581E-4 17 3.4253390828791624E-5 18 5.48054253260666E-4 19 5.138008624318743E-4 20 2.0552034497274972E-4 21 2.3977373580154132E-4 22 0.0029800450021048707 23 3.0828051745912456E-4 24 6.850678165758325E-5 25 0.002363483967186622 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2919419.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.39945721592613 #Duplication Level Percentage of deduplicated Percentage of total 1 91.48715866490083 71.72543581555539 2 6.579361988744119 10.316368174892704 3 0.9498116182421925 2.23394145982705 4 0.31699715247022586 0.9940961877063953 5 0.15764878542402952 0.617978960399696 6 0.09526764289964763 0.4481358896143832 7 0.07239113046004408 0.39727977347203264 8 0.04754222116077011 0.2981827467075115 9 0.03890671906388885 0.27452390909952706 >10 0.21776905229234678 3.272203035311839 >50 0.01976865638360683 1.0773842348732734 >100 0.014211807517503562 2.159375736432755 >500 0.0016262324490448335 0.8934694800718601 >1k 0.0012306623935578955 2.1868620877199296 >5k 8.790445668270682E-5 0.48941135497110416 >10k+ 2.1976114170676705E-4 2.6153511533444935 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 24031 0.8231432350066913 No Hit GGTATCAACGCAGAGTACTTTTTTT 16681 0.571380812415073 No Hit TATCAACGCAGAGTACTTTTTTTTT 14449 0.49492724408521005 No Hit GTACATGGGAAGCAGTGGTATCAAC 10376 0.35541318323954185 No Hit CCCATGTACTCTGCGTTGATACCAC 10362 0.35493363576793874 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 9151 0.3134527794742721 No Hit GAGTACATGGGAAGCAGTGGTATCA 5052 0.17304813046705525 No Hit CATGTACTCTGCGTTGATACCACTG 3848 0.13180704790919015 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 3707 0.12697731980233054 No Hit GTATCAACGCAGAGTACATGGGAAG 3618 0.12392876801856807 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 3429 0.11745487715192646 No Hit GTGGTATCAACGCAGAGTACATGGG 3396 0.11632451525457634 No Hit ATTCCATTCCATTCCATTCCATTCC 3271 0.11204284140097738 No Hit GGTATCAACGCAGAGTACATGGGAA 3261 0.11170030749268947 No Hit GCGTTGATACCACTGCTTCCCATGT 3244 0.11111799984860002 No Hit ACGCAGAGTACATGGGAAGCAGTGG 3129 0.10717885990328899 No Hit GTGGTATCAACGCAGAGTACTTTTT 2927 0.10025967495587307 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.425339082879162E-5 0.0 4 0.0 0.0 0.0 3.425339082879162E-5 0.0 5 0.0 0.0 0.0 3.425339082879162E-5 0.0 6 0.0 0.0 0.0 3.425339082879162E-5 0.0 7 0.0 0.0 0.0 3.425339082879162E-5 0.0 8 0.0 0.0 0.0 3.425339082879162E-5 0.0 9 0.0 0.0 0.0 6.850678165758323E-5 0.0 10 0.0 0.0 0.0 6.850678165758323E-5 0.0 11 0.0 0.0 0.0 6.850678165758323E-5 0.0 12 0.0 0.0 0.0 1.3701356331516647E-4 0.0 13 0.0 0.0 0.0 1.3701356331516647E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCGCG 55 0.0030207701 12.116799 7 TCGCGCG 55 0.0030234945 12.11534 9 CGCGCGC 70 0.0014671644 10.879081 10 TAACCCG 115 8.5849024E-7 10.762125 5 TATTCCG 115 8.5849024E-7 10.762125 5 GTCTAGC 160 5.366019E-10 10.697508 1 CCCGTAC 105 4.1899708E-5 9.934402 16 TGTATCG 125 2.5025201E-6 9.9011545 5 GGACTGT 315 0.0 9.671458 6 TTATACT 490 0.0 9.520342 4 GTCCTAG 280 0.0 9.508896 1 GTATACG 100 2.730903E-4 9.508896 1 TCGCTCT 205 4.0745363E-10 9.288138 4 ATTACAC 340 0.0 9.220513 3 CAGGACT 385 0.0 9.149419 4 CCGTACA 125 2.8149472E-5 9.103525 17 CCCCGTA 115 1.1330619E-4 9.070696 15 CGCTCTG 210 6.4210326E-10 9.066992 5 CTAGGAC 325 0.0 9.061465 3 AGCACCG 95 0.0017892877 9.019271 5 >>END_MODULE