FastQCFastQC Report
Wed 25 May 2016
SRR1294593_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294593_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4126829
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC252110.6109048860517361No Hit
CCCATGTACTCTGCGTTGATACCAC245270.5943304168890934No Hit
GAGTACATGGGAAGCAGTGGTATCA112150.2717582918991797No Hit
CATGTACTCTGCGTTGATACCACTG88790.21515308727354585No Hit
GCGTTGATACCACTGCTTCCCATGT82240.19928133683271101No Hit
GTATCAACGCAGAGTACATGGGAAG74890.18147105198688873No Hit
ATTCCATTCCATTCCATTCCATTCC69590.1686282615538468No Hit
ACGCAGAGTACATGGGAAGCAGTGG63900.15484043559837346No Hit
GTATCAACGCAGAGTACTTTTTTTT63600.15411348519650317No Hit
TATCAACGCAGAGTACATGGGAAGC53910.13063298721609254No Hit
ACTCTGCGTTGATACCACTGCTTCC52530.12728901536748918No Hit
GCTTCCCATGTACTCTGCGTTGATA52040.12610166304443438No Hit
GAATGGAATGGAATGGAATGGAATG51490.12476892064100548No Hit
GGTATCAACGCAGAGTACATGGGAA50160.12154610719271382No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCGTA4450.010.89267415
CCCGTAC4200.010.86255716
AGCGTCG1106.0159964E-610.36880517
CCGTACA4400.010.36880517
GTGCGCT951.650945E-49.99661811
TAACACG1356.4786764E-79.8487594
GTACTGG11300.09.6679191
GCCCCGT4050.09.62173114
CGCAGAA1606.673508E-89.4973632
TATACTG7100.09.4970185
GTCTTGC6100.09.34404851
TTAGACA5000.09.1171374
CTGTCGC2501.8189894E-129.1170269
TGTGCGC1252.7670792E-59.11702610
CGTCGGT1151.11395595E-49.08509519
TCTGTCG2304.1836756E-119.0839948
TTACACC4000.09.0221664
GTTCTAC2950.09.0167421
GCTATAC3500.08.9546573
ATCGCCG850.00745161768.93934419
Produced by FastQC (version 0.11.5)