##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294593_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4126829 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.18360586300038 31.0 30.0 33.0 26.0 34.0 2 30.278674982656174 31.0 30.0 34.0 26.0 34.0 3 30.333102001561006 31.0 30.0 34.0 26.0 34.0 4 33.915211170610654 35.0 35.0 37.0 28.0 37.0 5 34.23473591951593 35.0 35.0 37.0 30.0 37.0 6 34.30309760835741 37.0 35.0 37.0 30.0 37.0 7 34.27477634765094 37.0 35.0 37.0 30.0 37.0 8 34.18767993536926 37.0 35.0 37.0 29.0 37.0 9 35.34353882848065 39.0 35.0 39.0 28.0 39.0 10 35.44564700887776 39.0 35.0 39.0 28.0 39.0 11 35.666303595327065 39.0 35.0 39.0 30.0 39.0 12 35.45159588633307 39.0 35.0 39.0 28.0 39.0 13 35.313815522765786 39.0 35.0 39.0 27.0 39.0 14 36.240789477829104 39.0 36.0 41.0 27.0 41.0 15 36.20499662089222 39.0 36.0 41.0 27.0 41.0 16 36.309235735234004 39.0 36.0 41.0 27.0 41.0 17 36.28080688586806 39.0 36.0 41.0 27.0 41.0 18 36.14888816570786 39.0 36.0 41.0 27.0 41.0 19 36.41081687658975 39.0 36.0 41.0 27.0 41.0 20 36.54086660726674 39.0 36.0 41.0 27.0 41.0 21 36.56550756040534 39.0 36.0 41.0 28.0 41.0 22 36.564496372396334 39.0 36.0 41.0 28.0 41.0 23 36.528742043830746 39.0 36.0 41.0 28.0 41.0 24 36.58425415736877 39.0 36.0 41.0 28.0 41.0 25 35.307463672471044 39.0 34.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9928614591723459 1101 2 0.9985971324967196 1101 3 1.3257848342075178 1101 4 1.0456298975025788 1101 5 0.7659779642499629 1101 6 0.7726406029805446 1101 7 0.7954405503509108 1101 8 0.9692409098646024 1101 9 1.2733009513452842 1101 10 1.1897419210857834 1101 11 1.002336754537069 1101 12 1.2490106957924212 1101 13 1.3158614300665619 1101 14 1.3408518583232762 1101 15 1.4636009896844797 1101 16 1.4842677615185806 1101 17 1.395152285297634 1101 18 1.4762265550467433 1101 19 1.3406167215121911 1101 20 1.2283346096126593 1101 21 1.149198912449819 1101 22 1.0853039426427245 1101 23 1.1310630909479826 1101 24 1.1947823931813417 1101 25 1.0953150571994499 1102 1 0.9032498262176887 1102 2 0.9140608970668751 1102 3 1.186391437784593 1102 4 1.0245842078782204 1102 5 0.8282190385067736 1102 6 0.7734598103836703 1102 7 0.7599329967323598 1102 8 0.813387687451609 1102 9 1.194702274846243 1102 10 1.1040698388458168 1102 11 0.8192071394468456 1102 12 1.0528846445902644 1102 13 1.0394385247983209 1102 14 1.3320618639559854 1102 15 1.3629898657686468 1102 16 1.2779726736816457 1102 17 1.2574095520426098 1102 18 1.4330731581061542 1102 19 1.1520804176764017 1102 20 0.4310248736596023 1102 21 0.608696954560763 1102 22 0.6559016811791807 1102 23 0.8516998701742651 1102 24 0.873826740570145 1102 25 1.017476765154143 1103 1 0.6811780496409874 1103 2 0.7002892285371871 1103 3 0.9700949171971551 1103 4 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0.25571139930827513 2207 7 0.18544677054274672 2207 8 0.25604379767965924 2207 9 -1.4272550548682332 2207 10 -0.33537778240433624 2207 11 -0.0697867255550193 2207 12 0.27582441337150243 2207 13 0.43804840653584165 2207 14 0.6876112139467097 2207 15 0.5944566186806028 2207 16 0.6327861995564064 2207 17 0.6424177226486165 2207 18 0.6206307690960884 2207 19 -0.6314888575579758 2207 20 0.10889826213669807 2207 21 -0.5376879631579499 2207 22 0.09416201124349044 2207 23 -0.6357298403092315 2207 24 -1.8372737496662168 2207 25 -0.4237195829606364 2208 1 0.4106570794121289 2208 2 0.14399003469890914 2208 3 0.6291665015219969 2208 4 0.5261865965313888 2208 5 0.4286006039836323 2208 6 0.2970789885583969 2208 7 0.3418501503345581 2208 8 0.49473200512652227 2208 9 -0.1917812023451475 2208 10 0.021043773770188068 2208 11 0.23012059051433909 2208 12 0.3123251617804357 2208 13 0.5220758906993623 2208 14 0.8162957077320812 2208 15 0.6654372481965751 2208 16 0.4531838165757307 2208 17 0.41286514200814395 2208 18 0.7630514309010863 2208 19 -1.3736890316953065 2208 20 -0.12293802645348961 2208 21 -0.2599512592195623 2208 22 0.14524460647277238 2208 23 0.24760693294265224 2208 24 0.37985312826361195 2208 25 0.4348600141836627 2209 1 0.15165148799190575 2209 2 -1.4514320260588889 2209 3 -0.26311472693812377 2209 4 0.09947821468736606 2209 5 0.12756060134216796 2209 6 0.16836348955273905 2209 7 0.21680564379605727 2209 8 0.33933209003974696 2209 9 -1.9839761242212361 2209 10 -0.23885467883926026 2209 11 -0.020788927640126076 2209 12 0.3459899933519921 2209 13 0.509749947541458 2209 14 0.5912463708847824 2209 15 0.7545572619196079 2209 16 0.6495880288639739 2209 17 0.4435670604711248 2209 18 0.736986228882003 2209 19 0.438035594313952 2209 20 0.35241660364380323 2209 21 0.427728995955853 2209 22 0.3522966096167508 2209 23 0.360877772889161 2209 24 0.45175228886797925 2209 25 0.47161052921252633 2210 1 0.18332054757553706 2210 2 0.17084543266329177 2210 3 0.09903052312169791 2210 4 0.2622103276330492 2210 5 0.1598715609018697 2210 6 0.1389760762959753 2210 7 0.041031535157628696 2210 8 0.20546453799380515 2210 9 0.31929798441719726 2210 10 -1.5862625515360804 2210 11 -0.5050219977525927 2210 12 -2.311595553429541 2210 13 -0.8239572790677769 2210 14 -0.3580501115272767 2210 15 -0.6859410630385128 2210 16 -0.12354429944423373 2210 17 0.06273133422082822 2210 18 0.30045057441921585 2210 19 0.24221243087772137 2210 20 0.2266036101046751 2210 21 0.27433344678141225 2210 22 0.21186349164594276 2210 23 0.1528196250068916 2210 24 0.1194479098299297 2210 25 0.09307200324937526 2211 1 -0.29280644258910726 2211 2 -0.023535420096219895 2211 3 -1.3784777635706895 2211 4 -0.5441497314152954 2211 5 -0.43143968364159235 2211 6 -0.3224159068951664 2211 7 -0.46450633004550923 2211 8 -0.019698795882916897 2211 9 0.11684172728520537 2211 10 0.12871675437418872 2211 11 -0.16106883794169136 2211 12 -0.20843211993137345 2211 13 -0.20461645601356793 2211 14 -0.8163031137551755 2211 15 -0.3098507969587132 2211 16 -0.16197426557294392 2211 17 -0.21599790376573225 2211 18 -0.017906803906782898 2211 19 -0.08848555314661866 2211 20 0.04403550526669875 2211 21 -0.06340424091443708 2211 22 -0.18972374956472748 2211 23 -0.4003198087810773 2211 24 -0.23050952464633667 2211 25 0.012370260779853481 2212 1 -0.3015810219908346 2212 2 -0.284979919824071 2212 3 -2.374767029418873 2212 4 -1.368464718921885 2212 5 -0.6224049754109799 2212 6 -0.43218380665161504 2212 7 -0.23184422967344176 2212 8 -0.269639878074301 2212 9 -0.8621418932098308 2212 10 -0.2265478667794909 2212 11 -0.2987676747943553 2212 12 -0.04954447601533474 2212 13 -0.3258693567480151 2212 14 -0.09406766938841571 2212 15 0.17165204683112734 2212 16 -1.157377765791665 2212 17 -1.2638814617355436 2212 18 -0.22098265250284044 2212 19 -0.02654388729200008 2212 20 -0.15029915658859494 2212 21 -0.08338403127125815 2212 22 -0.36813916863604845 2212 23 -0.002148451609230051 2212 24 -0.12165344691388924 2212 25 0.06225596174898129 2213 1 -3.0305894753571465 2213 2 -1.322461258285042 2213 3 -2.291302000822725 2213 4 -0.8553132795435303 2213 5 -0.6801786886597156 2213 6 -0.5156077629054252 2213 7 -0.2655487966918244 2213 8 -0.13227625520733 2213 9 -0.20815075428508578 2213 10 -0.0889035201048074 2213 11 -0.4036231039174325 2213 12 -0.1344270656378228 2213 13 -0.5438496977916429 2213 14 -0.09625731508859303 2213 15 -0.11112688524275427 2213 16 -1.5905293257493724 2213 17 -0.8658957398382583 2213 18 -0.06933527476465429 2213 19 -0.0659333940244764 2213 20 -0.0633184862126015 2213 21 0.17300874692980273 2213 22 0.05968353751131872 2213 23 0.030173597757460868 2213 24 0.1142408411667688 2213 25 0.17123030623062618 2214 1 -0.8296510931016279 2214 2 -0.572939901926965 2214 3 -6.434265037060033 2214 4 -2.111401787270651 2214 5 -1.2491387759796524 2214 6 -0.6400211349063483 2214 7 -0.5363506610532696 2214 8 -0.38267066632162994 2214 9 -0.1303118130349148 2214 10 -0.033121520859303644 2214 11 -0.6130855509165016 2214 12 -0.3984391058078742 2214 13 -5.121112345383555 2214 14 -3.665362129543176 2214 15 -1.9686004042014318 2214 16 -2.6837658321045907 2214 17 -3.167599295418988 2214 18 -0.9541149688726165 2214 19 -0.6625529435150881 2214 20 -0.32688870433553774 2214 21 -0.3776230322633296 2214 22 -0.3189401534304537 2214 23 -0.1653923367093313 2214 24 -0.11544479347080028 2214 25 0.12250151082364624 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1594.0 3 194.0 4 194.0 5 428.0 6 50.0 7 55.0 8 109.0 9 329.0 10 790.0 11 1950.0 12 4156.0 13 7479.0 14 11351.0 15 15602.0 16 19679.0 17 22332.0 18 23844.0 19 24733.0 20 26184.0 21 26937.0 22 28276.0 23 30216.0 24 32376.0 25 35972.0 26 40929.0 27 46496.0 28 54149.0 29 67215.0 30 82989.0 31 103839.0 32 136143.0 33 180519.0 34 243735.0 35 331782.0 36 464635.0 37 703823.0 38 1355745.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.87942908038126 21.797042363935546 13.610568862740227 22.712959692942967 2 14.007166178585203 21.80668288944549 41.71376355780716 22.472387374162142 3 18.1424132843744 28.018169276853566 29.60484126127852 24.234576177493516 4 11.833543003063708 17.960788325860946 38.88544759537629 31.320221075699056 5 12.893277460662253 38.585235117355644 34.902026859125954 13.619460562856148 6 30.20061647569111 38.771917573048874 18.09267891470793 12.93478703655208 7 25.117052493345405 33.37418148119231 24.436438616969326 17.072327408492953 8 23.343669525061326 38.34128247820319 21.962649566246068 16.352398430489416 9 24.150063079527246 17.713720542782177 22.69517251880929 35.44104385888129 10 14.852167435833586 30.66807075090946 33.269643580666994 21.210118232589966 11 31.540416518563525 27.42173486523301 22.705920747322384 18.331927868881078 12 20.507304796276742 28.42313143056655 32.3533555025101 18.71620827064661 13 25.8392945079282 24.615725183769378 27.35471750906269 22.19026279923973 14 19.70679367440922 23.74986755209355 28.844547498547012 27.698791274950214 15 23.372215050342714 30.24139382541587 24.253733876607246 22.132657247634167 16 20.925395694285598 32.046030230827625 26.605441413193358 20.42313266169342 17 19.92528512113344 30.787505588946445 29.45061990023791 19.8365893896822 18 20.20876304920722 30.337156085747424 29.962023227710183 19.492057637335172 19 20.444376118596644 29.441160573798097 30.199132935292162 19.915330372313093 20 21.983484460277136 28.710062006620863 28.90908063721847 20.397372895883528 21 21.830219078804603 29.905717676753596 28.37006872656429 19.893994517877513 22 20.493393279655802 29.2928406302996 29.358405910186235 20.855360179858366 23 20.010591309306967 29.9234797300574 29.33353798225859 20.732390978377047 24 20.376578047229753 29.3041630717913 29.060560252536057 21.258698628442897 25 21.321654097197538 28.500970171294277 27.89590433010169 22.281471401406495 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3997.0 1 3997.0 2 3991.0 3 3985.0 4 3985.0 5 3985.0 6 5775.5 7 7566.0 8 7566.0 9 7566.0 10 11732.5 11 15899.0 12 15899.0 13 15899.0 14 26673.5 15 37448.0 16 37448.0 17 37448.0 18 60516.5 19 83585.0 20 83585.0 21 83585.0 22 121282.0 23 158979.0 24 158979.0 25 158979.0 26 209668.5 27 260358.0 28 260358.0 29 260358.0 30 314312.5 31 368267.0 32 368267.0 33 368267.0 34 414424.0 35 460581.0 36 460581.0 37 460581.0 38 488854.5 39 517128.0 40 517128.0 41 517128.0 42 509675.0 43 502222.0 44 502222.0 45 502222.0 46 525328.0 47 548434.0 48 548434.0 49 548434.0 50 492009.0 51 435584.0 52 435584.0 53 435584.0 54 365564.0 55 295544.0 56 295544.0 57 295544.0 58 245812.0 59 196080.0 60 196080.0 61 196080.0 62 160487.5 63 124895.0 64 124895.0 65 124895.0 66 93647.0 67 62399.0 68 62399.0 69 62399.0 70 45210.5 71 28022.0 72 28022.0 73 28022.0 74 19368.0 75 10714.0 76 10714.0 77 10714.0 78 7159.5 79 3605.0 80 3605.0 81 3605.0 82 2356.5 83 1108.0 84 1108.0 85 1108.0 86 706.0 87 304.0 88 304.0 89 304.0 90 195.5 91 87.0 92 87.0 93 87.0 94 50.0 95 13.0 96 13.0 97 13.0 98 19.0 99 25.0 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09879255961417349 2 0.052728135815658946 3 0.055345157262392024 4 0.05059574797017274 5 0.04783333644306561 6 0.0664190350508829 7 0.06326891664277827 8 0.06023995663498536 9 0.06469858576645653 10 0.06469858576645653 11 0.059391847832803345 12 0.06019149327486067 13 0.060627663515982845 14 0.062178491039972826 15 0.0653528411281398 16 0.060482273435608795 17 0.06341430672315233 18 0.1427003638871395 19 0.06784870417456115 20 0.06280851472159375 21 0.06091844367673097 22 0.0653043777680151 23 0.06525591440789041 24 0.0703445672209825 25 0.0682121793754963 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4126829.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.47513820298272 #Duplication Level Percentage of deduplicated Percentage of total 1 87.1981645407057 64.06897190674779 2 9.317883525899072 13.692655596494607 3 1.9380606232195008 4.2719781641043495 4 0.61036121673069 1.7938549901211256 5 0.26940950980180245 0.9897450482942635 6 0.13983802017174707 0.6164770714890362 7 0.09929886502131782 0.5107198481588459 8 0.06485950040579529 0.3812448604873778 9 0.051155039600198605 0.33827612439632826 >10 0.26834482286895434 3.601255186132535 >50 0.022410173922789853 1.1380234042247448 >100 0.01649837178986065 2.405282237976946 >500 0.0018247183681226252 0.9289199945625156 >1k 0.0014265977940862713 2.0627496647015615 >5k 3.649436217429829E-4 1.7140177254056028 >10k+ 9.953007865717714E-5 1.4858281767023311 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 25211 0.6109048860517361 No Hit CCCATGTACTCTGCGTTGATACCAC 24527 0.5943304168890934 No Hit GAGTACATGGGAAGCAGTGGTATCA 11215 0.2717582918991797 No Hit CATGTACTCTGCGTTGATACCACTG 8879 0.21515308727354585 No Hit GCGTTGATACCACTGCTTCCCATGT 8224 0.19928133683271101 No Hit GTATCAACGCAGAGTACATGGGAAG 7489 0.18147105198688873 No Hit ATTCCATTCCATTCCATTCCATTCC 6959 0.1686282615538468 No Hit ACGCAGAGTACATGGGAAGCAGTGG 6390 0.15484043559837346 No Hit GTATCAACGCAGAGTACTTTTTTTT 6360 0.15411348519650317 No Hit TATCAACGCAGAGTACATGGGAAGC 5391 0.13063298721609254 No Hit ACTCTGCGTTGATACCACTGCTTCC 5253 0.12728901536748918 No Hit GCTTCCCATGTACTCTGCGTTGATA 5204 0.12610166304443438 No Hit GAATGGAATGGAATGGAATGGAATG 5149 0.12476892064100548 No Hit GGTATCAACGCAGAGTACATGGGAA 5016 0.12154610719271382 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 2.4231680062343267E-5 0.0 9 0.0 0.0 0.0 7.26950401870298E-5 0.0 10 0.0 0.0 0.0 9.692672024937307E-5 0.0 11 0.0 0.0 0.0 9.692672024937307E-5 0.0 12 0.0 0.0 0.0 9.692672024937307E-5 0.0 13 0.0 0.0 0.0 9.692672024937307E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCGTA 445 0.0 10.892674 15 CCCGTAC 420 0.0 10.862557 16 AGCGTCG 110 6.0159964E-6 10.368805 17 CCGTACA 440 0.0 10.368805 17 GTGCGCT 95 1.650945E-4 9.996618 11 TAACACG 135 6.4786764E-7 9.848759 4 GTACTGG 1130 0.0 9.667919 1 GCCCCGT 405 0.0 9.621731 14 CGCAGAA 160 6.673508E-8 9.497363 2 TATACTG 710 0.0 9.497018 5 GTCTTGC 610 0.0 9.3440485 1 TTAGACA 500 0.0 9.117137 4 CTGTCGC 250 1.8189894E-12 9.117026 9 TGTGCGC 125 2.7670792E-5 9.117026 10 CGTCGGT 115 1.11395595E-4 9.085095 19 TCTGTCG 230 4.1836756E-11 9.083994 8 TTACACC 400 0.0 9.022166 4 GTTCTAC 295 0.0 9.016742 1 GCTATAC 350 0.0 8.954657 3 ATCGCCG 85 0.0074516176 8.939344 19 >>END_MODULE