FastQCFastQC Report
Wed 25 May 2016
SRR1294593_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294593_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4126829
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC271660.6582778205736172No Hit
GTACATGGGAAGCAGTGGTATCAAC240970.5839107944622857No Hit
GTATCAACGCAGAGTACTTTTTTTT173590.42063773420221673No Hit
GAGTACATGGGAAGCAGTGGTATCA117880.2856430445749024No Hit
GGTATCAACGCAGAGTACTTTTTTT113160.2742056915854764No Hit
TATCAACGCAGAGTACTTTTTTTTT106220.2573889056222102No Hit
CATGTACTCTGCGTTGATACCACTG97970.237397769570777No Hit
GCGTTGATACCACTGCTTCCCATGT88770.21510462391342117No Hit
GTATCAACGCAGAGTACATGGGAAG75290.18244031918938244No Hit
ACGCAGAGTACATGGGAAGCAGTGG74830.18132566190651467No Hit
ACGCAGAGTACTTTTTTTTTTTTTT65890.1596625399307798No Hit
ATTCCATTCCATTCCATTCCATTCC65220.15803901736660278No Hit
TATCAACGCAGAGTACATGGGAAGC59640.14451773989181524No Hit
ACTCTGCGTTGATACCACTGCTTCC58540.1418522550849575No Hit
GAATGGAATGGAATGGAATGGAATG57290.13882329507716457No Hit
GCTTCCCATGTACTCTGCGTTGATA55210.13378310562419718No Hit
GGTATCAACGCAGAGTACATGGGAA53630.12995450017434693No Hit
GTGGTATCAACGCAGAGTACATGGG44010.10664362395437271No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGCG550.003025727612.1143424
TGTACCG1053.390569E-610.8781855
TCGGAAC700.001514148110.83824813
TGTCCCG4750.010.58912311
GTCCCGC4850.010.55916812
TTCCGAA1106.1771934E-610.34585116
CCCGTAC4650.010.19752416
TAGGACC2900.010.1748544
CGCCCTA4950.010.15426216
CCCCGTA4550.010.00465815
CCGTACA4800.09.87885117
CCTAGAC3400.09.78064253
TCCCGCC5250.09.75442413
CGCCTTT3600.09.74689714
CCGCCCT5300.09.66251815
CTAGACA5400.09.5184124
GACCGTG800.0044465279.5184127
GGAACGA1701.7151251E-89.483582515
CCCGCCC5550.09.22715714
GAGCCGC1252.6942595E-59.1376756
Produced by FastQC (version 0.11.5)