##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294593_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4126829 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.366283894971176 31.0 31.0 34.0 28.0 34.0 2 31.597213986816513 31.0 31.0 34.0 29.0 34.0 3 31.672336799028987 31.0 31.0 34.0 29.0 34.0 4 35.29672443418421 37.0 35.0 37.0 32.0 37.0 5 35.45942344594361 37.0 35.0 37.0 33.0 37.0 6 35.049753212454405 37.0 35.0 37.0 32.0 37.0 7 34.93316781480405 37.0 35.0 37.0 32.0 37.0 8 34.95054047550795 37.0 35.0 37.0 32.0 37.0 9 36.477380090137004 39.0 37.0 39.0 32.0 39.0 10 36.22985614378497 39.0 37.0 39.0 32.0 39.0 11 36.407304979198315 39.0 37.0 39.0 32.0 39.0 12 36.37142028419399 39.0 37.0 39.0 32.0 39.0 13 36.23567707796955 39.0 35.0 39.0 31.0 39.0 14 37.356178799751575 40.0 37.0 41.0 31.0 41.0 15 37.39043512585571 40.0 37.0 41.0 31.0 41.0 16 37.42053014554274 40.0 37.0 41.0 31.0 41.0 17 37.4617140666599 40.0 37.0 41.0 32.0 41.0 18 37.4663803612895 40.0 37.0 41.0 32.0 41.0 19 37.48109044498815 40.0 37.0 41.0 31.0 41.0 20 37.54859457467222 40.0 37.0 41.0 32.0 41.0 21 37.39717080596264 40.0 37.0 41.0 31.0 41.0 22 37.393013861247944 40.0 37.0 41.0 31.0 41.0 23 37.400845055610496 40.0 37.0 41.0 31.0 41.0 24 37.34105168883906 40.0 37.0 41.0 31.0 41.0 25 36.3102066986541 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5036790548614647 1101 2 0.5130050100161796 1101 3 0.45213266260888574 1101 4 0.4132961466878129 1101 5 0.42464839324875925 1101 6 0.4701351851023148 1101 7 0.5574255454001857 1101 8 0.5860439296225266 1101 9 0.722547550009871 1101 10 0.7610571344796142 1101 11 0.7056808198952709 1101 12 0.839392913125593 1101 13 0.8381254859978782 1101 14 1.0187020268055207 1101 15 0.9755791757821157 1101 16 1.0312033299336107 1101 17 1.0194039893591125 1101 18 0.9958925713976683 1101 19 1.1340370021443604 1101 20 1.0186687174769844 1101 21 1.2821102022901414 1101 22 1.1945264933856947 1101 23 1.1715464305786298 1101 24 1.275785434960305 1101 25 1.1650040434329938 1102 1 0.43757595662683 1102 2 0.4179212866867914 1102 3 0.38015840851100435 1102 4 0.33291229073716977 1102 5 0.3628913988950231 1102 6 0.47123893966425356 1102 7 0.46097063724286613 1102 8 0.4804304823696697 1102 9 0.6237174611314416 1102 10 0.7469527531713211 1102 11 0.6619461727158722 1102 12 0.6709988936539304 1102 13 0.7467627224963351 1102 14 0.9405491752636763 1102 15 0.861068364561568 1102 16 0.8847782954533159 1102 17 0.8329790257498431 1102 18 0.8754771254587013 1102 19 1.0002317396935538 1102 20 0.908931470255439 1102 21 1.1431555591288216 1102 22 1.080216555296154 1102 23 0.9714336840245039 1102 24 1.1499185899040825 1102 25 1.1454580771035126 1103 1 0.2889120425864462 1103 2 0.28965918181148353 1103 3 0.24137721740471463 1103 4 0.21951066455493873 1103 5 0.2051648860501203 1103 6 0.31647625098653265 1103 7 0.2930171572079132 1103 8 0.29150772851520657 1103 9 0.285912489724069 1103 10 0.45465732674660586 1103 11 0.48122450455961996 1103 12 0.5176446438462534 1103 13 0.6393620622643539 1103 14 0.7402673256681069 1103 15 0.7418135902030656 1103 16 0.7563035819397257 1103 17 0.7008804116892904 1103 18 0.7512186416520592 1103 19 0.8850536861828928 1103 20 0.7551226885726408 1103 21 0.9466267250158467 1103 22 0.8903608378913788 1103 23 0.9702826970374474 1103 24 1.056351681666868 1103 25 0.94839572974481 1104 1 0.2138007535193438 1104 2 0.23737732589702532 1104 3 0.11531696646322942 1104 4 0.19872160778171377 1104 5 0.18084152667156417 1104 6 0.1849512172857004 1104 7 0.22856245855995638 1104 8 0.22740626327073699 1104 9 0.018688943646914424 1104 10 0.4086157922228395 1104 11 0.438996887760041 1104 12 0.4677922069482321 1104 13 0.014171122650381562 1104 14 0.31802951354853093 1104 15 0.5090025925562571 1104 16 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0.20817282043632446 2207 3 0.2073096785941395 2207 4 0.16367550281839982 2207 5 0.13104549347213634 2207 6 0.3382961069057586 2207 7 0.27200882073871213 2207 8 0.24963451742814868 2207 9 0.26441861299906577 2207 10 0.4796850695616044 2207 11 0.35782650268626526 2207 12 0.2996674487949562 2207 13 0.3974883920516703 2207 14 0.37541298088778063 2207 15 0.36221788430368207 2207 16 0.4898766186007819 2207 17 0.46619283266527134 2207 18 0.5086104002516763 2207 19 0.6198069738354164 2207 20 0.4812707992796348 2207 21 0.8374102789451285 2207 22 0.7066245761077212 2207 23 0.564549654976588 2207 24 0.7848353107675408 2207 25 0.747006236721468 2208 1 0.18330898628787295 2208 2 0.1937925149988935 2208 3 0.15201196738177103 2208 4 0.14278296990013217 2208 5 0.133004684926604 2208 6 0.25923829850497526 2208 7 0.2539732659376881 2208 8 0.1790832617165421 2208 9 0.3692723376107381 2208 10 0.46088501041304397 2208 11 0.06250756560966408 2208 12 -0.24940985849054442 2208 13 0.05209328349032205 2208 14 -0.40914580820361834 2208 15 0.151226191072368 2208 16 0.24442860159086166 2208 17 0.25439808419491783 2208 18 0.3054136442840871 2208 19 0.5475839149181354 2208 20 0.37663957914419655 2208 21 0.6731689617454037 2208 22 0.6351375546893578 2208 23 0.620761806167387 2208 24 0.6568947348188914 2208 25 0.6355747174288808 2209 1 0.009964952525702842 2209 2 0.016874770513279458 2209 3 0.08165211585015086 2209 4 -0.5608298167829844 2209 5 0.0035462585303065453 2209 6 0.10143367216693377 2209 7 0.12697901482147245 2209 8 0.1462845641884769 2209 9 0.26187841559731595 2209 10 0.28702001546108136 2209 11 0.29190427889978565 2209 12 0.32566751019254525 2209 13 0.3657188302391958 2209 14 0.39533598650043444 2209 15 -0.21171848339817245 2209 16 -0.21218723114241556 2209 17 0.15202516403201116 2209 18 -0.21517278841998433 2209 19 0.2614633456251454 2209 20 0.2856011221452448 2209 21 0.5552110333409956 2209 22 0.5519547251648333 2209 23 0.5426163891579066 2209 24 0.6382733173366262 2209 25 0.6672475110720342 2210 1 -0.13863219990310682 2210 2 -0.10265980713687739 2210 3 -0.07352735292228374 2210 4 0.03629748639959729 2210 5 -0.02800794103897175 2210 6 -0.45132117348991585 2210 7 -0.06599777388395722 2210 8 -0.1901918655794077 2210 9 0.0670566879112684 2210 10 0.34423705302987884 2210 11 0.2106378275956473 2210 12 0.1531393934181935 2210 13 0.2952139247594161 2210 14 0.241555775923743 2210 15 0.14545308874827612 2210 16 0.26805505170037236 2210 17 -0.8315259247452076 2210 18 -1.4076817618586048 2210 19 -0.2913854220226213 2210 20 -0.08448083526082684 2210 21 -0.33739724615170275 2210 22 -0.25690744281794053 2210 23 -0.16515401045923284 2210 24 0.29766611465655757 2210 25 0.21505260798517156 2211 1 -0.26080680005888013 2211 2 -0.25139488656233056 2211 3 -0.17090015445826623 2211 4 -0.17932940414361553 2211 5 -0.13332745416182945 2211 6 -0.10819618481053794 2211 7 -0.17741167379158185 2211 8 -0.08143994107434338 2211 9 -0.03954984363250702 2211 10 -0.040062789387114606 2211 11 -0.2971736252471828 2211 12 -0.22759300528491622 2211 13 -0.13439103095432614 2211 14 -0.16286700840490198 2211 15 -0.18210256340989162 2211 16 -0.20890445671311397 2211 17 -0.27069417629839876 2211 18 -0.45055258002231113 2211 19 -0.19460400957174784 2211 20 -0.3048724833616845 2211 21 0.04926815260574813 2211 22 -0.0514394031867198 2211 23 -0.6481622609256377 2211 24 -0.13854949330590927 2211 25 -0.8329187825489797 2212 1 -0.22755545076769934 2212 2 -0.3698226163946572 2212 3 -0.173581888703076 2212 4 -0.05755593206276188 2212 5 -0.08329791177587254 2212 6 -0.09691508144415906 2212 7 -0.16164821283048525 2212 8 -0.39268321966294195 2212 9 -0.14234474864100832 2212 10 -0.4875825798512068 2212 11 -0.16569563875178517 2212 12 -0.34289811655786195 2212 13 -0.35883236414720443 2212 14 -0.30178088276208115 2212 15 -0.17591429619452725 2212 16 -0.4129990850381233 2212 17 -0.5166921923695753 2212 18 -0.3672734085731548 2212 19 -0.4306631554623621 2212 20 -0.6382125458221495 2212 21 -0.09852786599740426 2212 22 -0.35109871709301643 2212 23 -0.18557899050711768 2212 24 -0.48037392258738976 2212 25 -0.13273337232874383 2213 1 -0.23408444595733613 2213 2 -0.2110983610286894 2213 3 -0.1339934260370832 2213 4 -0.08235420657221226 2213 5 -0.12066206217750874 2213 6 -0.3288016132578946 2213 7 -0.3488589636856574 2213 8 -0.4135366652556556 2213 9 -0.35699295460862857 2213 10 -0.3719601680681919 2213 11 -0.18862191879582468 2213 12 -0.03885237779454087 2213 13 -0.01632256519066999 2213 14 -0.7016612077535811 2213 15 -0.2900170313110806 2213 16 -0.44904025384393975 2213 17 -0.3582060812270669 2213 18 -0.2738944962441394 2213 19 -0.37270405145739716 2213 20 -0.43974759635620586 2213 21 -0.14862481933395344 2213 22 -0.49169160577491056 2213 23 -0.684133168694764 2213 24 -0.8550814234521695 2213 25 -0.6263128985573871 2214 1 -0.2949690225067698 2214 2 -0.2325391556318408 2214 3 -0.19627130725408648 2214 4 -0.17566927060840243 2214 5 -0.2520217802837692 2214 6 -0.26374654369298867 2214 7 -0.2714346200549258 2214 8 -0.33433205176111613 2214 9 -0.34691505750947726 2214 10 -0.26919277532158503 2214 11 -0.16763262686053793 2214 12 -0.3846771613037774 2214 13 -0.36966136492203105 2214 14 -0.31759760140189286 2214 15 -0.1679142862468197 2214 16 -0.39105040543987 2214 17 -0.6469562244427607 2214 18 -0.4607843320040672 2214 19 -0.7663011034450378 2214 20 -0.6159324830081871 2214 21 -0.7377830952947733 2214 22 -0.8810769572005412 2214 23 -1.100148609618664 2214 24 -0.6568451583706576 2214 25 -1.0660756439754948 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 14.0 9 41.0 10 120.0 11 211.0 12 525.0 13 1228.0 14 2600.0 15 4497.0 16 6776.0 17 8826.0 18 10692.0 19 12055.0 20 13605.0 21 15074.0 22 16995.0 23 19204.0 24 21999.0 25 25479.0 26 29411.0 27 33708.0 28 40659.0 29 50756.0 30 65613.0 31 87061.0 32 118037.0 33 161555.0 34 223809.0 35 317344.0 36 457265.0 37 736300.0 38 1645368.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.72961495185291 22.014525258635516 12.883884520725406 24.37197526878617 2 13.868323596640423 22.29154636647169 40.27419115257744 23.565938884310448 3 17.982163806186982 27.78539790532089 29.796779973771017 24.435658314721106 4 11.776633679874793 18.259878467333188 38.75316324705583 31.210324605736183 5 13.152847585297403 38.073608964404485 35.07115782064647 13.702385629651642 6 30.478311362118387 39.01303538175046 17.560686211499483 12.947967044631667 7 25.154102969601123 33.5668781318355 24.149782778615965 17.129236119947404 8 23.675663413431792 38.225667327589825 21.57903194025662 16.51963731872177 9 24.127319216908695 17.06178892048704 22.93507064997199 35.87582121263228 10 14.818598977935382 30.44623311023259 33.306233319442754 21.428934592389275 11 31.88214418555254 26.844218868433266 22.574668574773323 18.698968371240866 12 20.622653424310318 28.181880304103125 32.417141979248605 18.778324292337956 13 26.20757453791364 24.08446588249662 27.492387612174586 22.21557196741515 14 19.88557678677227 23.234333748911077 29.14838064013512 27.731708824181535 15 23.538203709824533 29.70425795270492 24.54053676820527 22.21700156926528 16 20.954772505552693 31.646379081647737 26.815208588876775 20.58363982392279 17 19.77921536328031 30.620813520896323 29.673499761438993 19.926471354384372 18 20.072191283639146 29.943510902114806 30.445883919168637 19.538413895077415 19 20.436957041668716 29.071392379543248 30.802267997120296 19.68938258166774 20 21.858558023932694 28.18965097364244 29.612440368777627 20.33935063364724 21 21.685538321963815 29.553962078344025 28.864763798989244 19.895735800702912 22 20.26377894464674 29.0578239872751 29.90336376675577 20.775033301322384 23 19.812184866618978 29.522533551224868 30.0390324375355 20.626249144620658 24 20.19967389004972 28.987001884497758 29.76495997289929 21.04836425255323 25 21.15902484888211 28.172179284157366 28.56146914820276 22.10732671875776 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1802.0 1 1802.0 2 2089.5 3 2377.0 4 2377.0 5 2377.0 6 4805.5 7 7234.0 8 7234.0 9 7234.0 10 10906.5 11 14579.0 12 14579.0 13 14579.0 14 23607.5 15 32636.0 16 32636.0 17 32636.0 18 55203.0 19 77770.0 20 77770.0 21 77770.0 22 116843.0 23 155916.0 24 155916.0 25 155916.0 26 208441.5 27 260967.0 28 260967.0 29 260967.0 30 316433.5 31 371900.0 32 371900.0 33 371900.0 34 417149.5 35 462399.0 36 462399.0 37 462399.0 38 489466.5 39 516534.0 40 516534.0 41 516534.0 42 508916.5 43 501299.0 44 501299.0 45 501299.0 46 527785.0 47 554271.0 48 554271.0 49 554271.0 50 498183.0 51 442095.0 52 442095.0 53 442095.0 54 369940.0 55 297785.0 56 297785.0 57 297785.0 58 247208.5 59 196632.0 60 196632.0 61 196632.0 62 161249.0 63 125866.0 64 125866.0 65 125866.0 66 93991.0 67 62116.0 68 62116.0 69 62116.0 70 44929.0 71 27742.0 72 27742.0 73 27742.0 74 18960.5 75 10179.0 76 10179.0 77 10179.0 78 6752.0 79 3325.0 80 3325.0 81 3325.0 82 2186.0 83 1047.0 84 1047.0 85 1047.0 86 653.5 87 260.0 88 260.0 89 260.0 90 170.0 91 80.0 92 80.0 93 80.0 94 47.5 95 15.0 96 15.0 97 15.0 98 9.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09552128280575715 2 0.0 3 0.1316749494587733 4 0.012867022113104276 5 0.024231680062343268 6 0.012624705312480842 7 0.02069385477324115 8 0.018949173808752433 9 1.2115840031171633E-4 10 0.3908569994055969 11 0.06472281744651887 12 0.0017204492844263718 13 7.26950401870298E-5 14 0.0010661939227431037 15 0.001381205763553566 16 7.26950401870298E-5 17 4.8463360124686534E-5 18 3.63475200935149E-4 19 3.877068809974923E-4 20 1.453900803740596E-4 21 1.2115840031171633E-4 22 0.002810874887231819 23 1.2115840031171633E-4 24 0.0 25 0.0020354611252368345 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4126829.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.20290517465442 #Duplication Level Percentage of deduplicated Percentage of total 1 86.09746656950519 61.30389747926457 2 10.116570892424814 14.40658475891986 3 2.0818394393857393 4.446990485743156 4 0.6855640289371427 1.9525660217426164 5 0.31261272099443116 1.112946696467859 6 0.15884312903431805 0.6786055352565566 7 0.09893219439539785 0.49309817593791944 8 0.06947378120219347 0.3957388044051576 9 0.05166889407695287 0.3311077828896484 >10 0.28159567268406444 3.675829094237356 >50 0.023552687094415003 1.1677498015647096 >100 0.017682068675008298 2.476448712088829 >500 0.002116025640154092 1.0502473707709559 >1k 0.0015016954395530194 2.1928875425212535 >5k 3.75423859888253E-4 1.8281272196722935 >10k+ 2.04776650848138E-4 2.487174518517306 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 27166 0.6582778205736172 No Hit GTACATGGGAAGCAGTGGTATCAAC 24097 0.5839107944622857 No Hit GTATCAACGCAGAGTACTTTTTTTT 17359 0.42063773420221673 No Hit GAGTACATGGGAAGCAGTGGTATCA 11788 0.2856430445749024 No Hit GGTATCAACGCAGAGTACTTTTTTT 11316 0.2742056915854764 No Hit TATCAACGCAGAGTACTTTTTTTTT 10622 0.2573889056222102 No Hit CATGTACTCTGCGTTGATACCACTG 9797 0.237397769570777 No Hit GCGTTGATACCACTGCTTCCCATGT 8877 0.21510462391342117 No Hit GTATCAACGCAGAGTACATGGGAAG 7529 0.18244031918938244 No Hit ACGCAGAGTACATGGGAAGCAGTGG 7483 0.18132566190651467 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 6589 0.1596625399307798 No Hit ATTCCATTCCATTCCATTCCATTCC 6522 0.15803901736660278 No Hit TATCAACGCAGAGTACATGGGAAGC 5964 0.14451773989181524 No Hit ACTCTGCGTTGATACCACTGCTTCC 5854 0.1418522550849575 No Hit GAATGGAATGGAATGGAATGGAATG 5729 0.13882329507716457 No Hit GCTTCCCATGTACTCTGCGTTGATA 5521 0.13378310562419718 No Hit GGTATCAACGCAGAGTACATGGGAA 5363 0.12995450017434693 No Hit GTGGTATCAACGCAGAGTACATGGG 4401 0.10664362395437271 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.4231680062343267E-5 0.0 2 0.0 0.0 0.0 2.4231680062343267E-5 0.0 3 0.0 0.0 0.0 4.8463360124686534E-5 0.0 4 0.0 0.0 0.0 9.692672024937307E-5 0.0 5 0.0 0.0 0.0 9.692672024937307E-5 0.0 6 0.0 0.0 0.0 9.692672024937307E-5 0.0 7 0.0 0.0 0.0 1.2115840031171633E-4 0.0 8 0.0 0.0 0.0 1.2115840031171633E-4 0.0 9 0.0 0.0 0.0 1.2115840031171633E-4 0.0 10 0.0 0.0 0.0 1.453900803740596E-4 0.0 11 0.0 0.0 0.0 1.6962176043640287E-4 0.0 12 0.0 0.0 0.0 1.9385344049874614E-4 0.0 13 0.0 0.0 0.0 1.9385344049874614E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGGCG 55 0.0030257276 12.114342 4 TGTACCG 105 3.390569E-6 10.878185 5 TCGGAAC 70 0.0015141481 10.838248 13 TGTCCCG 475 0.0 10.589123 11 GTCCCGC 485 0.0 10.559168 12 TTCCGAA 110 6.1771934E-6 10.345851 16 CCCGTAC 465 0.0 10.197524 16 TAGGACC 290 0.0 10.174854 4 CGCCCTA 495 0.0 10.154262 16 CCCCGTA 455 0.0 10.004658 15 CCGTACA 480 0.0 9.878851 17 CCTAGAC 340 0.0 9.7806425 3 TCCCGCC 525 0.0 9.754424 13 CGCCTTT 360 0.0 9.746897 14 CCGCCCT 530 0.0 9.662518 15 CTAGACA 540 0.0 9.518412 4 GACCGTG 80 0.004446527 9.518412 7 GGAACGA 170 1.7151251E-8 9.4835825 15 CCCGCCC 555 0.0 9.227157 14 GAGCCGC 125 2.6942595E-5 9.137675 6 >>END_MODULE