FastQCFastQC Report
Wed 25 May 2016
SRR1294592_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294592_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4485181
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC252890.5638345475912789No Hit
CCCATGTACTCTGCGTTGATACCAC238880.5325983499885512No Hit
GAGTACATGGGAAGCAGTGGTATCA112740.2513610933427213No Hit
GTATCAACGCAGAGTACTTTTTTTT107410.23947751495424602No Hit
CATGTACTCTGCGTTGATACCACTG87680.19548820883705698No Hit
GCGTTGATACCACTGCTTCCCATGT81350.18137506602297654No Hit
GTATCAACGCAGAGTACATGGGAAG77360.1724791039648121No Hit
ACGCAGAGTACATGGGAAGCAGTGG62770.1399497589952334No Hit
GGTATCAACGCAGAGTACTTTTTTT60750.13544603885551107No Hit
TATCAACGCAGAGTACTTTTTTTTT59060.1316780749762384No Hit
TATCAACGCAGAGTACATGGGAAGC55820.12445428623727782No Hit
ACTCTGCGTTGATACCACTGCTTCC52750.1176095234506701No Hit
GGTATCAACGCAGAGTACATGGGAA51000.11370778570586114No Hit
GTGGTATCAACGCAGAGTACATGGG45750.10200257247143427No Hit
ATTCCATTCCATTCCATTCCATTCC45020.10037499044074252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATCG2800.011.88096613
CGCTATC3150.011.16408712
TGCGCTA3150.011.15512110
GCGCTAT3050.010.89774811
TTGTGCG3200.010.3872637
GTGCGCT3750.010.383199
CGCCCTA4650.010.22018516
CGGTCTC3550.09.90737818
GCGCCTT3800.09.75489913
TATCGCG3650.09.6270227
CGGACTG800.00452991069.4970325
CCCCGTA6250.09.27665915
TCGGTCT3400.09.22532417
CGCCTTT4650.09.19816714
TGTGCGC4200.09.0445918
TAGACAG5300.08.9594655
TGCGCAA850.00745908738.93828410
TCGCGCG3850.08.8801449
CCGTACA6650.08.86169217
GTATTAG6450.08.8365191
Produced by FastQC (version 0.11.5)