##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294592_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4485181 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.891227132193773 31.0 30.0 33.0 25.0 34.0 2 29.984579217650303 31.0 30.0 34.0 25.0 34.0 3 29.992032205612215 31.0 30.0 34.0 25.0 34.0 4 33.600030411258764 35.0 35.0 37.0 28.0 37.0 5 34.02751795301015 35.0 35.0 37.0 28.0 37.0 6 34.21060822294574 37.0 35.0 37.0 30.0 37.0 7 34.130047594511794 37.0 35.0 37.0 29.0 37.0 8 33.93892331212497 37.0 35.0 37.0 28.0 37.0 9 35.12511713574101 39.0 35.0 39.0 27.0 39.0 10 35.22838855332706 39.0 35.0 39.0 27.0 39.0 11 35.466669684010526 39.0 35.0 39.0 28.0 39.0 12 35.25439642235174 39.0 35.0 39.0 27.0 39.0 13 35.106747085569125 38.0 35.0 39.0 27.0 39.0 14 35.99741526596139 39.0 35.0 41.0 27.0 41.0 15 35.980688181814735 39.0 35.0 41.0 27.0 41.0 16 36.08440751889388 39.0 35.0 41.0 27.0 41.0 17 36.07094585480497 39.0 35.0 41.0 27.0 41.0 18 35.93816503726382 39.0 35.0 41.0 27.0 41.0 19 36.19845464430532 39.0 35.0 41.0 27.0 41.0 20 36.3262296437981 39.0 36.0 41.0 27.0 41.0 21 36.36121039485363 39.0 36.0 41.0 27.0 41.0 22 36.36839628099735 39.0 36.0 41.0 27.0 41.0 23 36.33631819986752 39.0 36.0 41.0 27.0 41.0 24 36.35815656045988 39.0 35.0 41.0 27.0 41.0 25 35.122974747284445 38.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9003695436253736 1101 2 0.8829582747694822 1101 3 1.1991362451735519 1101 4 1.053184261495744 1101 5 0.7018925972152985 1101 6 0.6558157290484132 1101 7 0.6994023160343588 1101 8 0.8429102218931988 1101 9 1.0876997744837595 1101 10 0.9765588830444898 1101 11 0.8281941584019421 1101 12 1.037382884414889 1101 13 1.144029349042178 1101 14 1.1834426661992765 1101 15 1.3316208294931045 1101 16 1.2390417646525265 1101 17 1.1797874901256549 1101 18 1.2186273462817994 1101 19 1.1250941711785316 1101 20 1.0208431723728069 1101 21 0.9547716036372123 1101 22 0.8779472779758564 1101 23 0.8803033378008536 1101 24 0.96213790748957 1101 25 1.0057364630365626 1102 1 0.7558365427922027 1102 2 0.7188505159536902 1102 3 1.0602010789893903 1102 4 0.9654351157201688 1102 5 0.7441122783755247 1102 6 0.6402574034405646 1102 7 0.61558854319928 1102 8 0.7531934678900711 1102 9 1.0374805599629795 1102 10 0.9330129881669222 1102 11 0.6367914346719417 1102 12 0.8774161203477959 1102 13 0.9142179112039059 1102 14 1.1624418959390468 1102 15 1.2240842620839487 1102 16 1.0180884955337604 1102 17 1.1204632902970957 1102 18 1.2787782584488596 1102 19 0.9310232541103503 1102 20 0.08615532493855227 1102 21 0.4607567983193235 1102 22 0.5892603621774057 1102 23 0.7435341642029627 1102 24 0.7843221063690464 1102 25 0.7738238175652938 1103 1 0.6598693881098185 1103 2 0.6585904169833405 1103 3 0.9132549953190328 1103 4 0.82791119539921 1103 5 0.5278315995006153 1103 6 0.44109475644430063 1103 7 0.4434312530187725 1103 8 0.5646739557286793 1103 9 0.697618534595982 1103 10 0.6605067957227035 1103 11 0.5744008598959738 1103 12 0.7949216004453632 1103 13 0.689330771927473 1103 14 0.5550351298421461 1103 15 0.8609747221231672 1103 16 0.8253664876887754 1103 17 0.8766494034059349 1103 18 0.926981202825381 1103 19 0.7787373671036022 1103 20 -0.14468926960880424 1103 21 0.30368144820133125 1103 22 -0.2506776364749683 1103 23 0.26800533032142226 1103 24 0.4648777072392747 1103 25 0.3881693605357981 1104 1 0.329089015853107 1104 2 0.34388731439672426 1104 3 0.5845855299392966 1104 4 0.5268565936531928 1104 5 0.3070957010760438 1104 6 0.19997586998316308 1104 7 0.2617968654966347 1104 8 0.42245331472943803 1104 9 0.6805273637562834 1104 10 0.618393467974407 1104 11 0.41085244582477287 1104 12 0.4969164448669545 1104 13 0.4424425723072787 1104 14 0.7770332881217072 1104 15 0.7604411755053917 1104 16 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0.31365985628635684 2207 8 0.34717235617878117 2207 9 -1.343030048868357 2207 10 -0.2671572527789152 2207 11 0.13282273134708333 2207 12 0.484058739148729 2207 13 0.6338706038126745 2207 14 0.8156031939686343 2207 15 0.8354966014421379 2207 16 0.7829433563910939 2207 17 0.786592690516855 2207 18 0.8215825920214783 2207 19 -0.3393882424696528 2207 20 0.30778606169472056 2207 21 -0.36690823520902427 2207 22 0.28171806124301213 2207 23 -0.4835054711557518 2207 24 -1.5079497130788937 2207 25 -0.08497626258599666 2208 1 0.45345758829007465 2208 2 0.19143700687092036 2208 3 0.6081580109016365 2208 4 0.4929844669003529 2208 5 0.4099516434952477 2208 6 0.2959409808850566 2208 7 0.3079885512034082 2208 8 0.4953816828726829 2208 9 -0.14988071042142792 2208 10 0.09640671734552342 2208 11 0.3155578692483516 2208 12 0.24444704835273967 2208 13 0.551596683259028 2208 14 0.7739462980616665 2208 15 0.6453149730946564 2208 16 0.5223759634547207 2208 17 0.449636537562526 2208 18 0.6763500432543808 2208 19 -1.3872288108513757 2208 20 -0.0925920924798973 2208 21 -0.19409414394591096 2208 22 0.17125877930246958 2208 23 0.4070349338644945 2208 24 0.47511095279486426 2208 25 0.5825316969513423 2209 1 0.08352698673897763 2209 2 -1.4878629580345368 2209 3 -0.3600662343296257 2209 4 0.07085998199198684 2209 5 0.07922546843828826 2209 6 0.08024657232531496 2209 7 0.07415565133861435 2209 8 0.23704552551822644 2209 9 -2.2090604692780715 2209 10 -0.46719489051371 2209 11 -0.06558342990048516 2209 12 0.26686082243161025 2209 13 0.335589972561948 2209 14 0.5560476334776681 2209 15 0.7301317357359665 2209 16 0.634390773014232 2209 17 0.3899788107929254 2209 18 0.5850427426267615 2209 19 0.42682273510848745 2209 20 0.31556296750670754 2209 21 0.4135816436544104 2209 22 0.3670210574122521 2209 23 0.35198639296233836 2209 24 0.4331610891754494 2209 25 0.44010587355982267 2210 1 0.1860142114051655 2210 2 0.2519649029294442 2210 3 0.07328642132975105 2210 4 0.3219323141908177 2210 5 0.1696825093102916 2210 6 0.15502615515201512 2210 7 0.15948556082357612 2210 8 0.21314714732173456 2210 9 0.37857187416447147 2210 10 -1.4796417352997224 2210 11 -0.5200864878503069 2210 12 -2.2652489398070585 2210 13 -0.7149231939716287 2210 14 -0.4393593757746288 2210 15 -0.7823760654709275 2210 16 -0.07969576926719668 2210 17 0.10856339760304223 2210 18 0.3744596743397679 2210 19 0.22112393265530983 2210 20 0.30628545358893433 2210 21 0.308753692426329 2210 22 0.22184199435506002 2210 23 0.1087551604782675 2210 24 0.12451318011225965 2210 25 0.10136411436711512 2211 1 -0.3085714439470735 2211 2 -0.03056478410954 2211 3 -1.4409260255324199 2211 4 -0.6218459585210923 2211 5 -0.5809283832572447 2211 6 -0.4215618756564581 2211 7 -0.4290889587003477 2211 8 -0.09906845969255329 2211 9 0.09224412505624713 2211 10 0.06365813339711224 2211 11 -0.2835716274666851 2211 12 -0.21962474148560318 2211 13 -0.23267525650098975 2211 14 -0.7451088662312415 2211 15 -0.2948960045033715 2211 16 -0.11812701477970933 2211 17 -0.19760893847868743 2211 18 -0.005827743600491431 2211 19 -0.07295302330125963 2211 20 -0.1113407427609161 2211 21 -0.2004771115272206 2211 22 -0.19624192018238062 2211 23 -0.5164176834556997 2211 24 -0.3356731959058905 2211 25 -0.09871334368860119 2212 1 -0.2997476218258939 2212 2 -0.31694382356162976 2212 3 -2.4123313982944943 2212 4 -1.3835489429768586 2212 5 -0.6592296813559315 2212 6 -0.5066973261072292 2212 7 -0.24820105218854138 2212 8 -0.40901745804483625 2212 9 -0.8500366368948349 2212 10 -0.22533068021029123 2212 11 -0.305830099462149 2212 12 -0.06549643093028834 2212 13 -0.3870520138795399 2212 14 -0.11370124603599407 2212 15 0.006914170921426432 2212 16 -1.1676585981648202 2212 17 -1.3140029577922263 2212 18 -0.1753737809560718 2212 19 -0.09234936330954469 2212 20 -0.20935035364558985 2212 21 -0.10912988987632133 2212 22 -0.2957444887722218 2212 23 -0.02132495867903117 2212 24 -0.16431184992668335 2212 25 0.030697825327941075 2213 1 -2.940568599456274 2213 2 -1.3597612921519584 2213 3 -2.3202406634542214 2213 4 -0.9230362049342702 2213 5 -0.8559707743792728 2213 6 -0.5672863693667836 2213 7 -0.32537872181426053 2213 8 -0.20887461022929443 2213 9 -0.290380308278948 2213 10 -0.19267810904871396 2213 11 -0.48132025020792923 2213 12 -0.13178290869502263 2213 13 -0.6492007567190683 2213 14 -0.17207534347822673 2213 15 -0.3936614744756568 2213 16 -1.5760198873475701 2213 17 -0.9409379272457343 2213 18 -0.3044191326672703 2213 19 -0.19272233379061277 2213 20 -0.15540974225658033 2213 21 -0.0022878682415949925 2213 22 -0.05972715426701569 2213 23 0.04858908619949176 2213 24 0.1063256227243059 2213 25 0.13492929322310943 2214 1 -0.7620010966938899 2214 2 -0.5319739723489043 2214 3 -6.517937109553234 2214 4 -2.01110494150916 2214 5 -1.039426479029359 2214 6 -0.586328636997834 2214 7 -0.4930745046824683 2214 8 -0.37474721658654175 2214 9 -0.09075196385143158 2214 10 -0.07467768031957434 2214 11 -0.7551509118049751 2214 12 -0.4314234092747782 2214 13 -5.123798401319856 2214 14 -3.823028181979801 2214 15 -2.0617732699399625 2214 16 -2.804541293414104 2214 17 -3.315988600973064 2214 18 -1.182711200667086 2214 19 -0.6728102916192356 2214 20 -0.18847118175315103 2214 21 -0.2963162169551694 2214 22 -0.290125959195322 2214 23 -0.08212009473141535 2214 24 0.02014804843583562 2214 25 0.35618662855882377 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1700.0 3 240.0 4 248.0 5 495.0 6 71.0 7 78.0 8 161.0 9 431.0 10 1075.0 11 2616.0 12 5673.0 13 9859.0 14 15130.0 15 21323.0 16 25957.0 17 29146.0 18 30578.0 19 30951.0 20 31699.0 21 31850.0 22 32604.0 23 33971.0 24 36251.0 25 39490.0 26 44540.0 27 50805.0 28 59821.0 29 73575.0 30 90638.0 31 113981.0 32 149721.0 33 199059.0 34 268635.0 35 368835.0 36 517945.0 37 786617.0 38 1379412.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.198431319686236 20.618277152120346 13.157292740947918 23.0259987872455 2 15.11579297711177 21.213997057214776 40.42892120777331 23.241288757900147 3 19.17536885833774 27.11744283501644 28.45021608710676 25.25697221953906 4 12.344534750268519 17.69730186720025 37.33287381090206 32.625289571629175 5 13.346321563994735 38.514171098004404 34.1380935629963 14.001413775004556 6 31.144095590735432 37.58216000149929 17.725298007098445 13.54844640066683 7 26.418120927999478 33.08371373809843 22.68719071313372 17.81097462076837 8 24.6106001012848 36.82619394477524 20.97322645630364 17.589979497636314 9 24.97497865990923 17.538899650035898 21.43352756943394 36.05259412062093 10 15.407020197625727 30.29663938101963 32.28659522869299 22.009745192661654 11 32.2475489370417 26.618676467274653 22.074034181806663 19.059740413876984 12 21.604127420683326 27.955640846936873 30.653407138835075 19.786824593544726 13 26.871031568530036 23.957351485485365 26.240722897318335 22.930894048666264 14 20.721375347784697 23.598224928769813 27.611723358928085 28.068676364517405 15 24.066652491994397 29.673107218306832 23.759100775449767 22.501139514249004 16 21.65379213874818 31.05565923346269 25.811173342643894 21.47937528514523 17 20.75480243332659 29.928837222480897 28.070711665702497 21.24564867849002 18 21.125302344876676 29.281608701370004 28.91186210059385 20.68122685315947 19 21.435838511395456 28.707332582188954 29.265202306980065 20.591626599435525 20 22.96543160188671 27.802008878473604 28.09980228909163 21.13275723054805 21 22.75515241915675 28.54835424433603 27.781757532612485 20.914735803894736 22 21.7908778781193 28.230997847678463 28.142602513042327 21.835521761159914 23 21.27425598903394 28.675456116114617 28.370716827837803 21.67957106701364 24 21.51291100422442 28.476345537292296 28.0561792513137 21.95456420716958 25 22.138190414930012 27.639845579003993 27.35138273189867 22.870581274167325 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4488.0 1 4488.0 2 4398.5 3 4309.0 4 4309.0 5 4309.0 6 6482.0 7 8655.0 8 8655.0 9 8655.0 10 12294.0 11 15933.0 12 15933.0 13 15933.0 14 25442.0 15 34951.0 16 34951.0 17 34951.0 18 55862.0 19 76773.0 20 76773.0 21 76773.0 22 112098.0 23 147423.0 24 147423.0 25 147423.0 26 195594.5 27 243766.0 28 243766.0 29 243766.0 30 295692.5 31 347619.0 32 347619.0 33 347619.0 34 392110.0 35 436601.0 36 436601.0 37 436601.0 38 468020.5 39 499440.0 40 499440.0 41 499440.0 42 509049.0 43 518658.0 44 518658.0 45 518658.0 46 561841.0 47 605024.0 48 605024.0 49 605024.0 50 560338.5 51 515653.0 52 515653.0 53 515653.0 54 451161.0 55 386669.0 56 386669.0 57 386669.0 58 330824.5 59 274980.0 60 274980.0 61 274980.0 62 230475.0 63 185970.0 64 185970.0 65 185970.0 66 143086.0 67 100202.0 68 100202.0 69 100202.0 70 74368.0 71 48534.0 72 48534.0 73 48534.0 74 33933.5 75 19333.0 76 19333.0 77 19333.0 78 13230.5 79 7128.0 80 7128.0 81 7128.0 82 4662.0 83 2196.0 84 2196.0 85 2196.0 86 1386.0 87 576.0 88 576.0 89 576.0 90 396.0 91 216.0 92 216.0 93 216.0 94 130.0 95 44.0 96 44.0 97 44.0 98 42.0 99 40.0 100 40.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.099706121113061 2 0.052461650934488485 3 0.055694519351615915 4 0.05052192988421203 5 0.04789104386199799 6 0.06851451479884535 7 0.0645235944770122 8 0.06178123023351789 9 0.06704300227794598 10 0.06624035908472813 11 0.0610454739730682 12 0.061736638945005785 13 0.06184811716628604 14 0.06425604674593957 15 0.06706529792220203 16 0.0627845342250402 17 0.06514787251618162 18 0.14353935772045764 19 0.06980766216569632 20 0.06418915981317141 21 0.06180352587777394 22 0.06786794111541987 23 0.06664168068133705 24 0.07544846016247728 25 0.07018668811804919 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4485181.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.10779637809188 #Duplication Level Percentage of deduplicated Percentage of total 1 83.5602226776528 57.746628541152454 2 11.324105916414558 15.651680116710457 3 2.771114432443996 5.74516835813194 4 0.9353742414216191 2.5856661045390963 5 0.4568496014647717 1.5785934616719934 6 0.2309997813268954 0.9578331510793702 7 0.15024560645371857 0.7268199944254556 8 0.09888844837785779 0.5467170203713957 9 0.07319639376239853 0.4552597328168225 >10 0.3532779640383069 4.3335829782470014 >50 0.024594258113052533 1.1695770454432506 >100 0.01712166606171982 2.2830394888473435 >500 0.001825241778195647 0.8974974325746568 >1k 0.0017600539889947872 2.392687687325284 >5k 2.933423314989776E-4 1.325669485994855 >10k+ 1.3037436955510114E-4 1.6035794006685307 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 25289 0.5638345475912789 No Hit CCCATGTACTCTGCGTTGATACCAC 23888 0.5325983499885512 No Hit GAGTACATGGGAAGCAGTGGTATCA 11274 0.2513610933427213 No Hit GTATCAACGCAGAGTACTTTTTTTT 10741 0.23947751495424602 No Hit CATGTACTCTGCGTTGATACCACTG 8768 0.19548820883705698 No Hit GCGTTGATACCACTGCTTCCCATGT 8135 0.18137506602297654 No Hit GTATCAACGCAGAGTACATGGGAAG 7736 0.1724791039648121 No Hit ACGCAGAGTACATGGGAAGCAGTGG 6277 0.1399497589952334 No Hit GGTATCAACGCAGAGTACTTTTTTT 6075 0.13544603885551107 No Hit TATCAACGCAGAGTACTTTTTTTTT 5906 0.1316780749762384 No Hit TATCAACGCAGAGTACATGGGAAGC 5582 0.12445428623727782 No Hit ACTCTGCGTTGATACCACTGCTTCC 5275 0.1176095234506701 No Hit GGTATCAACGCAGAGTACATGGGAA 5100 0.11370778570586114 No Hit GTGGTATCAACGCAGAGTACATGGG 4575 0.10200257247143427 No Hit ATTCCATTCCATTCCATTCCATTCC 4502 0.10037499044074252 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.2295644256051207E-5 0.0 3 0.0 0.0 0.0 6.688693276815362E-5 0.0 4 0.0 0.0 0.0 6.688693276815362E-5 0.0 5 0.0 0.0 0.0 6.688693276815362E-5 0.0 6 0.0 0.0 0.0 6.688693276815362E-5 0.0 7 0.0 0.0 0.0 1.3377386553630724E-4 0.0 8 0.0 0.0 0.0 1.7836515404840965E-4 0.0 9 0.0 0.0 2.2295644256051207E-5 2.0066079830446083E-4 0.0 10 0.0 0.0 2.2295644256051207E-5 2.4525208681656327E-4 0.0 11 0.0 2.2295644256051207E-5 2.2295644256051207E-5 2.675477310726145E-4 0.0 12 0.0 2.2295644256051207E-5 2.2295644256051207E-5 2.675477310726145E-4 0.0 13 0.0 2.2295644256051207E-5 2.2295644256051207E-5 2.675477310726145E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTATCG 280 0.0 11.880966 13 CGCTATC 315 0.0 11.164087 12 TGCGCTA 315 0.0 11.155121 10 GCGCTAT 305 0.0 10.897748 11 TTGTGCG 320 0.0 10.387263 7 GTGCGCT 375 0.0 10.38319 9 CGCCCTA 465 0.0 10.220185 16 CGGTCTC 355 0.0 9.907378 18 GCGCCTT 380 0.0 9.754899 13 TATCGCG 365 0.0 9.627022 7 CGGACTG 80 0.0045299106 9.497032 5 CCCCGTA 625 0.0 9.276659 15 TCGGTCT 340 0.0 9.225324 17 CGCCTTT 465 0.0 9.198167 14 TGTGCGC 420 0.0 9.044591 8 TAGACAG 530 0.0 8.959465 5 TGCGCAA 85 0.0074590873 8.938284 10 TCGCGCG 385 0.0 8.880144 9 CCGTACA 665 0.0 8.861692 17 GTATTAG 645 0.0 8.836519 1 >>END_MODULE