FastQCFastQC Report
Wed 25 May 2016
SRR1294592_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294592_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4485181
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT299950.6687578494602558No Hit
CCCATGTACTCTGCGTTGATACCAC270790.6037437508096105No Hit
GTACATGGGAAGCAGTGGTATCAAC241530.5385066957164047No Hit
GGTATCAACGCAGAGTACTTTTTTT190970.4257799183578099No Hit
TATCAACGCAGAGTACTTTTTTTTT179660.40056354470421596No Hit
GAGTACATGGGAAGCAGTGGTATCA119960.26745854849559025No Hit
ACGCAGAGTACTTTTTTTTTTTTTT110500.24636686902936583No Hit
CATGTACTCTGCGTTGATACCACTG99000.22072687813490693No Hit
GCGTTGATACCACTGCTTCCCATGT88680.1977177732626621No Hit
ACGCAGAGTACATGGGAAGCAGTGG75050.16732881014166429No Hit
GTATCAACGCAGAGTACATGGGAAG74000.16498776749477892No Hit
ACTCTGCGTTGATACCACTGCTTCC59530.1327259702562728No Hit
TATCAACGCAGAGTACATGGGAAGC57480.12815536318378234No Hit
GGTATCAACGCAGAGTACATGGGAA57020.12712976354800398No Hit
GAGTACTTTTTTTTTTTTTTTTTTT51070.11386385521565351No Hit
GTACTTTTTTTTTTTTTTTTTTTTT50680.1129943250896675No Hit
GTGGTATCAACGCAGAGTACATGGG48870.10895881347932224No Hit
GCTTCCCATGTACTCTGCGTTGATA45460.10135599878800879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTA2550.010.82423110
GCGTAAG803.7306515E-410.7007661
CGCTATC2850.010.31478612
CGCCCTA4350.010.24594516
GCGCTAT2700.010.19209311
TATTCCG2350.010.12645
GACCGTC856.479159E-410.0787477
TTGTGCG2650.010.0576177
ACCGTCC1002.70318E-49.5188168
CAGCGTA1804.0563464E-99.51785810
ATCGCCG1502.7216265E-79.48305419
TAGACAG6200.09.3652875
AATCGCC1752.7534043E-89.212007518
GTGCGCT2900.09.1896569
CTTGGAC3850.09.132933
GTCCCGC5250.09.03138112
GTCGTAG950.00180437169.011171
CTAGCAC3950.08.9017173
TGTCCCG5250.08.85657811
TTATACT6900.08.8290474
Produced by FastQC (version 0.11.5)