##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294592_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4485181 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.26914164668048 31.0 31.0 34.0 28.0 34.0 2 31.49147960806933 31.0 31.0 34.0 28.0 34.0 3 31.571101143967212 31.0 31.0 34.0 28.0 34.0 4 35.18828939121966 37.0 35.0 37.0 32.0 37.0 5 35.3786353326655 37.0 35.0 37.0 32.0 37.0 6 34.92569642117007 37.0 35.0 37.0 32.0 37.0 7 34.798228655655144 37.0 35.0 37.0 32.0 37.0 8 34.80895553601962 37.0 35.0 37.0 32.0 37.0 9 36.298399328811925 39.0 37.0 39.0 32.0 39.0 10 36.06262422854284 39.0 35.0 39.0 30.0 39.0 11 36.23024622640647 39.0 37.0 39.0 31.0 39.0 12 36.19404166743772 39.0 35.0 39.0 31.0 39.0 13 36.0395685257741 39.0 35.0 39.0 30.0 39.0 14 37.13289028915444 40.0 37.0 41.0 31.0 41.0 15 37.1615063026442 40.0 37.0 41.0 31.0 41.0 16 37.19573190022878 40.0 37.0 41.0 31.0 41.0 17 37.2505809687502 40.0 37.0 41.0 31.0 41.0 18 37.25493151781389 40.0 37.0 41.0 31.0 41.0 19 37.269384892159316 40.0 37.0 41.0 31.0 41.0 20 37.33307975753933 40.0 37.0 41.0 31.0 41.0 21 37.217493563804894 40.0 37.0 41.0 30.0 41.0 22 37.20240654724971 40.0 37.0 41.0 30.0 41.0 23 37.20497946459686 40.0 37.0 41.0 30.0 41.0 24 37.12890873300319 40.0 36.0 41.0 30.0 41.0 25 36.05021246634194 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4520277291133077 1101 2 0.4508029314348647 1101 3 0.3772431244741803 1101 4 0.2920936605837383 1101 5 0.3732554997993631 1101 6 0.3909788665426248 1101 7 0.47609754610688526 1101 8 0.4852018432894596 1101 9 0.6477242246050494 1101 10 0.6908563132776777 1101 11 0.6212003667135022 1101 12 0.7240840958548134 1101 13 0.6803424126065849 1101 14 0.8687817093898431 1101 15 0.824012328589177 1101 16 0.8835592906134124 1101 17 0.8242203397664412 1101 18 0.8535015437501485 1101 19 0.9401197387278373 1101 20 0.8627796800672911 1101 21 1.1114791616812596 1101 22 1.1290876178591418 1101 23 1.070902675950542 1101 24 1.1755114533519873 1101 25 1.1648480856011574 1102 1 0.4046270121520017 1102 2 0.43318138502350934 1102 3 0.3695724842912611 1102 4 0.3116592641707925 1102 5 0.3559603036779819 1102 6 0.3915389668904439 1102 7 0.46639705563080014 1102 8 0.4934646633260513 1102 9 0.5904410637753443 1102 10 0.7703893107855251 1102 11 0.700619208126156 1102 12 0.7492598181943251 1102 13 0.7460574368201591 1102 14 1.0221117322746593 1102 15 0.9989680852780154 1102 16 0.9378028400213907 1102 17 0.8823171464042119 1102 18 0.8683692993126044 1102 19 1.001074046909622 1102 20 0.9023048001003886 1102 21 1.1657569813906008 1102 22 1.1419960589199576 1102 23 1.086718889488317 1102 24 1.102925077228626 1102 25 1.1873916844859025 1103 1 0.3307670736421997 1103 2 0.3051228582496037 1103 3 0.22965136600178582 1103 4 0.20681282886375385 1103 5 0.24299564119900907 1103 6 0.28326145993904817 1103 7 0.30511229610489465 1103 8 0.3003853946023298 1103 9 0.28493096530993256 1103 10 0.4860470553297844 1103 11 0.4875381464950408 1103 12 0.5108012710987353 1103 13 0.648367055768297 1103 14 0.8276423388661911 1103 15 0.7330620137385253 1103 16 0.7210746722810768 1103 17 0.637872207382479 1103 18 0.6699495669529725 1103 19 0.7864634985549586 1103 20 0.705076403430354 1103 21 0.920361638319946 1103 22 0.8612338309109191 1103 23 0.8067664605726677 1103 24 0.9899654640988871 1103 25 0.9443292572839255 1104 1 0.1469994029591568 1104 2 0.16588148912041234 1104 3 0.09550030466392911 1104 4 0.12165057628686782 1104 5 0.16338763652863975 1104 6 0.13461229581068324 1104 7 0.14607044496938215 1104 8 0.11567100321918389 1104 9 0.0047752449561855315 1104 10 0.39497379893783346 1104 11 0.4449281788025772 1104 12 0.3961311006208774 1104 13 -0.10170838353725742 1104 14 0.2759139967604227 1104 15 0.41330494223920056 1104 16 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0.19388449619206582 2207 2 0.20451438600408522 2207 3 0.2219324552890818 2207 4 0.1484255957708598 2207 5 0.11482426445788008 2207 6 0.337978857945167 2207 7 0.26358788175293313 2207 8 0.22916269519548393 2207 9 0.35839803239601764 2207 10 0.5170743894479273 2207 11 0.40566994148939983 2207 12 0.36214422498864707 2207 13 0.4006065988190741 2207 14 0.40020321701784667 2207 15 0.47273587380252735 2207 16 0.5106468440220127 2207 17 0.47676536833554906 2207 18 0.46777672748345367 2207 19 0.6157626012730262 2207 20 0.5010369028363044 2207 21 0.8147127158945295 2207 22 0.7816617531883807 2207 23 0.5987843230791725 2207 24 0.8110684384947078 2207 25 0.7719790050919357 2208 1 0.22698766604208132 2208 2 0.17973449755476167 2208 3 0.20036274173870083 2208 4 0.19760365697096205 2208 5 0.13927895296980353 2208 6 0.2857010525187107 2208 7 0.24904852269602884 2208 8 0.18756529079662698 2208 9 0.35727797998538335 2208 10 0.46673756769563823 2208 11 0.050293797701307597 2208 12 -0.1645720780745421 2208 13 0.055120830387487274 2208 14 -0.33496020069548393 2208 15 0.19970544339236085 2208 16 0.31912017804890525 2208 17 0.25533031319285016 2208 18 0.3641769171814673 2208 19 0.4820445296320415 2208 20 0.4624308739261025 2208 21 0.7648726798669045 2208 22 0.7134476043785369 2208 23 0.6887644441522411 2208 24 0.7241529853235917 2208 25 0.7911786884912004 2209 1 -0.031076445502641548 2209 2 -0.02010421296726861 2209 3 0.07339872622005217 2209 4 -0.5780637143918383 2209 5 -0.07883854987361616 2209 6 0.11725930856784572 2209 7 0.13386623914578877 2209 8 0.16852812723970345 2209 9 0.21299572505874664 2209 10 0.34443464813469404 2209 11 0.2955438432643618 2209 12 0.3583721584490078 2209 13 0.39425072423141216 2209 14 0.4108303129500044 2209 15 -0.17764115919333534 2209 16 -0.2778568008488307 2209 17 0.16007052849734293 2209 18 -0.2545560820009669 2209 19 0.2547042366763179 2209 20 0.3161359428850474 2209 21 0.5493642756454946 2209 22 0.5944886601285546 2209 23 0.6179788117618017 2209 24 0.698735263973866 2209 25 0.6516181010349626 2210 1 -0.16975172811788397 2210 2 -0.1550652137189097 2210 3 -0.05016800518903253 2210 4 0.01673875177752393 2210 5 -0.04911194365888605 2210 6 -0.47079377345695406 2210 7 -0.1366437532681175 2210 8 -0.24886487503903965 2210 9 -0.00450642151405134 2210 10 0.2290554456784335 2210 11 0.11167088884420195 2210 12 0.11242929276895097 2210 13 0.17073570828536333 2210 14 0.12505635860865993 2210 15 0.07025020203898436 2210 16 0.14865639621925908 2210 17 -0.9483051516201471 2210 18 -1.4940893903969936 2210 19 -0.32247682762433527 2210 20 -0.1280356981159727 2210 21 -0.2883248429097236 2210 22 -0.2416845667706653 2210 23 -0.2607023826483612 2210 24 0.18056046551915017 2210 25 0.1980235041552163 2211 1 -0.2658835858537607 2211 2 -0.2756253393966901 2211 3 -0.14845925595012943 2211 4 -0.06988507271966427 2211 5 -0.11994761378898033 2211 6 -0.13203789574534142 2211 7 -0.1741783642547574 2211 8 -0.14636173186169543 2211 9 -0.08779899327259955 2211 10 0.10643929289210519 2211 11 -0.4208545718880359 2211 12 -0.18725219479998856 2211 13 -0.17984450957711573 2211 14 -0.30537943525706623 2211 15 -0.3622839389014203 2211 16 -0.31685239516259145 2211 17 -0.4703089735670076 2211 18 -0.5563379385800218 2211 19 -0.32035965341204786 2211 20 -0.4229153299284718 2211 21 -0.16275391691611674 2211 22 -0.19811322187640457 2211 23 -0.7569453571641418 2211 24 -0.17214504409916032 2211 25 -0.8220454279509539 2212 1 -0.19361775627330857 2212 2 -0.3353839449600855 2212 3 -0.2093635775238738 2212 4 -0.08899743159772555 2212 5 -0.09555568658569769 2212 6 -0.17799115765573958 2212 7 -0.19786728765674155 2212 8 -0.4126634967671947 2212 9 -0.18272063935746985 2212 10 -0.5093397552340448 2212 11 -0.26558592295923233 2212 12 -0.2796399822006279 2212 13 -0.3688104926716349 2212 14 -0.417554859079452 2212 15 -0.19082965059568835 2212 16 -0.49341115035000627 2212 17 -0.6609026905176805 2212 18 -0.5071625363380221 2212 19 -0.5441352335909713 2212 20 -0.6810403630585569 2212 21 -0.15488190858454942 2212 22 -0.5052806410513995 2212 23 -0.36866512752926894 2212 24 -0.5532848614704236 2212 25 -0.34560567218506577 2213 1 -0.26102292656192105 2213 2 -0.2733139420753794 2213 3 -0.20160861202689162 2213 4 -0.08910325246426964 2213 5 -0.16750869868924667 2213 6 -0.33555274787030953 2213 7 -0.45290400737909664 2213 8 -0.41752437841979173 2213 9 -0.34961862091245166 2213 10 -0.3403645251564953 2213 11 -0.0684131003256283 2213 12 0.015818770189397924 2213 13 0.04170324677892978 2213 14 -0.7179046536961522 2213 15 -0.27711360960488207 2213 16 -0.43243848056349776 2213 17 -0.3904869772224657 2213 18 -0.29151068941481384 2213 19 -0.46269082639239656 2213 20 -0.5080316184445977 2213 21 -0.21400842285910926 2213 22 -0.5180438593630399 2213 23 -0.5814325200998596 2213 24 -0.8473107277349143 2213 25 -0.4738980893493192 2214 1 -0.2655320338538836 2214 2 -0.10457672674195351 2214 3 -0.057508296305631035 2214 4 -0.04902502202070025 2214 5 -0.0393506470334799 2214 6 -0.06206974360598849 2214 7 -0.11860163042026528 2214 8 -0.07683346312372663 2214 9 0.016438747874232718 2214 10 -0.0115949525351553 2214 11 0.029456179901458768 2214 12 -0.1587883456181487 2214 13 -0.22091099349449905 2214 14 -0.04198970464022267 2214 15 0.06798215581118683 2214 16 -0.1987953254594359 2214 17 -0.37203709108325 2214 18 -0.1726008893576534 2214 19 -0.37621926289014596 2214 20 -0.410155687435136 2214 21 -0.38060328570858104 2214 22 -0.643413478133418 2214 23 -0.9443198981526848 2214 24 -0.5029992927799682 2214 25 -0.7695390680464342 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 15.0 9 38.0 10 115.0 11 269.0 12 736.0 13 1662.0 14 3703.0 15 6381.0 16 9489.0 17 12660.0 18 14773.0 19 16368.0 20 18042.0 21 19147.0 22 21101.0 23 23360.0 24 26682.0 25 29907.0 26 34512.0 27 39145.0 28 46722.0 29 58302.0 30 74519.0 31 98615.0 32 131895.0 33 179219.0 34 246665.0 35 344783.0 36 495414.0 37 795655.0 38 1735285.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.044391459671964 20.71097838137967 12.466591920645435 24.778038238302933 2 14.813894913048104 21.665168027778588 39.027388192360576 24.493548866812734 3 18.764296899523522 26.73637979620095 28.95775462937123 25.5415686749043 4 12.294126067917716 17.662891874571596 37.334274021096796 32.70870803641389 5 13.406251442591707 37.876910883458216 34.41466848434151 14.302169189608568 6 31.382037640525358 37.79853903134005 17.036844436139152 13.782578891995446 7 26.326841556884517 33.2491828782667 22.559673971996634 17.864301592852147 8 24.891076854458785 36.618403813453014 20.70568951579182 17.78482981629638 9 25.017357186142615 16.669230669757738 21.61543788490703 36.69797425919262 10 15.385987636953407 29.908034816366207 32.41531545868774 22.29066208799264 11 32.57602394499932 25.84753892668718 22.066242298548595 19.510194829764906 12 21.835671219364716 27.61397389493136 30.84745377255298 19.70290111315095 13 27.448419015513316 23.16261554720212 26.477621856870847 22.911343580413718 14 20.97953643385204 22.84081529637189 28.101941922909017 28.077706346867053 15 24.29907938152168 28.892027155469275 24.25221347753703 22.556679985472016 16 21.61540113132659 30.439333832310293 26.276465789421465 21.668799246941646 17 20.536339559094717 29.511874771608994 28.56745357656692 21.38433209272937 18 20.87728185229017 28.539106192583024 29.770274138873603 20.8133378162532 19 21.174501477541238 28.072480900321374 30.363327546835627 20.38969007530176 20 22.632487672713555 27.01187379213105 29.39138120009667 20.964257335058722 21 22.384609193272674 27.990393231384044 28.847194454793364 20.77780312054992 22 21.374483728587723 27.631233941071702 29.2561232398166 21.738159090523972 23 20.900865026984437 28.01525379817702 29.576774879391632 21.507106295446913 24 21.19480154642623 27.908980241595653 29.265068514530075 21.63114969744804 25 21.819534575932742 27.044953595382417 28.506276540574 22.62923528811085 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2471.0 1 2471.0 2 2675.0 3 2879.0 4 2879.0 5 2879.0 6 6096.0 7 9313.0 8 9313.0 9 9313.0 10 12601.0 11 15889.0 12 15889.0 13 15889.0 14 22249.5 15 28610.0 16 28610.0 17 28610.0 18 49233.5 19 69857.0 20 69857.0 21 69857.0 22 107966.0 23 146075.0 24 146075.0 25 146075.0 26 196965.5 27 247856.0 28 247856.0 29 247856.0 30 300852.5 31 353849.0 32 353849.0 33 353849.0 34 397049.5 35 440250.0 36 440250.0 37 440250.0 38 468255.5 39 496261.0 40 496261.0 41 496261.0 42 505385.0 43 514509.0 44 514509.0 45 514509.0 46 559957.5 47 605406.0 48 605406.0 49 605406.0 50 563279.5 51 521153.0 52 521153.0 53 521153.0 54 455546.5 55 389940.0 56 389940.0 57 389940.0 58 333079.0 59 276218.0 60 276218.0 61 276218.0 62 232014.0 63 187810.0 64 187810.0 65 187810.0 66 144353.0 67 100896.0 68 100896.0 69 100896.0 70 74315.5 71 47735.0 72 47735.0 73 47735.0 74 33391.5 75 19048.0 76 19048.0 77 19048.0 78 12831.0 79 6614.0 80 6614.0 81 6614.0 82 4264.5 83 1915.0 84 1915.0 85 1915.0 86 1185.0 87 455.0 88 455.0 89 455.0 90 294.0 91 133.0 92 133.0 93 133.0 94 83.5 95 34.0 96 34.0 97 34.0 98 19.5 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09428827955884055 2 0.0 3 0.13517849112443847 4 0.011571439368890575 5 0.02372256548843848 6 0.016922393990342864 7 0.025617695250202837 8 0.023544200334390072 9 1.5606950979235845E-4 10 0.3904413222119687 11 0.06485802914085295 12 0.0016721733192038405 13 2.2295644256051207E-5 14 8.69530125985997E-4 15 9.81008347266253E-4 16 8.918257702420483E-5 17 0.0 18 2.0066079830446083E-4 19 6.019823949133825E-4 20 2.0066079830446083E-4 21 2.2295644256051207E-4 22 0.0030545032630790152 23 6.688693276815362E-5 24 2.2295644256051207E-5 25 0.0014938081651554307 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4485181.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.18829274183736 #Duplication Level Percentage of deduplicated Percentage of total 1 82.22838352462932 54.42556320416241 2 12.152042749696415 16.08645925856457 3 3.0349603308395396 6.026365285124129 4 1.075486531834404 2.8473846963583562 5 0.46858756356585074 1.5507505416240426 6 0.2623067374099085 1.041698107430597 7 0.16805681823422314 0.7786375717783958 8 0.10656728623797845 0.5642805390577994 9 0.07376906932014134 0.4394383879908966 >10 0.3817233913289475 4.5474012657316525 >50 0.025310963652625484 1.1707090545659742 >100 0.01836778826885433 2.398216759487054 >500 0.0019307099446990582 0.8989269043055399 >1k 0.0019645815155536045 2.682297243769405 >5k 3.048488558616828E-4 1.3732083087719802 >10k+ 2.371046656701977E-4 3.168662871277148 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 29995 0.6687578494602558 No Hit CCCATGTACTCTGCGTTGATACCAC 27079 0.6037437508096105 No Hit GTACATGGGAAGCAGTGGTATCAAC 24153 0.5385066957164047 No Hit GGTATCAACGCAGAGTACTTTTTTT 19097 0.4257799183578099 No Hit TATCAACGCAGAGTACTTTTTTTTT 17966 0.40056354470421596 No Hit GAGTACATGGGAAGCAGTGGTATCA 11996 0.26745854849559025 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 11050 0.24636686902936583 No Hit CATGTACTCTGCGTTGATACCACTG 9900 0.22072687813490693 No Hit GCGTTGATACCACTGCTTCCCATGT 8868 0.1977177732626621 No Hit ACGCAGAGTACATGGGAAGCAGTGG 7505 0.16732881014166429 No Hit GTATCAACGCAGAGTACATGGGAAG 7400 0.16498776749477892 No Hit ACTCTGCGTTGATACCACTGCTTCC 5953 0.1327259702562728 No Hit TATCAACGCAGAGTACATGGGAAGC 5748 0.12815536318378234 No Hit GGTATCAACGCAGAGTACATGGGAA 5702 0.12712976354800398 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 5107 0.11386385521565351 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 5068 0.1129943250896675 No Hit GTGGTATCAACGCAGAGTACATGGG 4887 0.10895881347932224 No Hit GCTTCCCATGTACTCTGCGTTGATA 4546 0.10135599878800879 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.2295644256051207E-5 0.0 4 0.0 0.0 0.0 2.2295644256051207E-5 0.0 5 0.0 2.2295644256051207E-5 0.0 2.2295644256051207E-5 0.0 6 0.0 2.2295644256051207E-5 0.0 2.2295644256051207E-5 0.0 7 0.0 2.2295644256051207E-5 0.0 2.2295644256051207E-5 0.0 8 0.0 2.2295644256051207E-5 0.0 2.2295644256051207E-5 0.0 9 0.0 2.2295644256051207E-5 0.0 1.1147822128025602E-4 0.0 10 0.0 2.2295644256051207E-5 0.0 1.1147822128025602E-4 0.0 11 2.2295644256051207E-5 2.2295644256051207E-5 0.0 1.1147822128025602E-4 0.0 12 2.2295644256051207E-5 2.2295644256051207E-5 0.0 1.1147822128025602E-4 0.0 13 2.2295644256051207E-5 2.2295644256051207E-5 0.0 1.1147822128025602E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGCTA 255 0.0 10.824231 10 GCGTAAG 80 3.7306515E-4 10.700766 1 CGCTATC 285 0.0 10.314786 12 CGCCCTA 435 0.0 10.245945 16 GCGCTAT 270 0.0 10.192093 11 TATTCCG 235 0.0 10.1264 5 GACCGTC 85 6.479159E-4 10.078747 7 TTGTGCG 265 0.0 10.057617 7 ACCGTCC 100 2.70318E-4 9.518816 8 CAGCGTA 180 4.0563464E-9 9.517858 10 ATCGCCG 150 2.7216265E-7 9.483054 19 TAGACAG 620 0.0 9.365287 5 AATCGCC 175 2.7534043E-8 9.2120075 18 GTGCGCT 290 0.0 9.189656 9 CTTGGAC 385 0.0 9.13293 3 GTCCCGC 525 0.0 9.031381 12 GTCGTAG 95 0.0018043716 9.01117 1 CTAGCAC 395 0.0 8.901717 3 TGTCCCG 525 0.0 8.856578 11 TTATACT 690 0.0 8.829047 4 >>END_MODULE