FastQCFastQC Report
Wed 25 May 2016
SRR1294591_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294591_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4840099
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC412130.8514908476045635No Hit
CCCATGTACTCTGCGTTGATACCAC402350.8312846493429162No Hit
GAGTACATGGGAAGCAGTGGTATCA187770.38794661018297355No Hit
CATGTACTCTGCGTTGATACCACTG146200.30205993720376384No Hit
GCGTTGATACCACTGCTTCCCATGT131720.2721431937652515No Hit
GTATCAACGCAGAGTACATGGGAAG119880.2476808842133188No Hit
GTATCAACGCAGAGTACTTTTTTTT114530.23662739129922755No Hit
ACGCAGAGTACATGGGAAGCAGTGG101570.2098510794923823No Hit
TATCAACGCAGAGTACATGGGAAGC90120.1861945385827852No Hit
ACTCTGCGTTGATACCACTGCTTCC85690.1770418332352293No Hit
GGTATCAACGCAGAGTACATGGGAA80740.16681476969789252No Hit
ATTCCATTCCATTCCATTCCATTCC80570.16646353721277188No Hit
GTACTGGTTCACTATCGGTCAGTCA79830.16493464286577608No Hit
GCTTCCCATGTACTCTGCGTTGATA68820.14218717427060892No Hit
GGTATCAACGCAGAGTACTTTTTTT67080.13859220648172693No Hit
GTGGTATCAACGCAGAGTACATGGG65710.13576168586634282No Hit
TATCAACGCAGAGTACTTTTTTTTT61660.12739408842670366No Hit
GAATGGAATGGAATGGAATGGAATG59800.12355119182479532No Hit
CAGTGGTATCAACGCAGAGTACATG53790.11113409043905921No Hit
GGATACCACGTGTCCCGCCCTACTC48810.10084504469846589No Hit
ATACCACTGCTTCCCATGTACTCTG48670.10055579441660181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG3150.011.15628919
TATTCCG3100.011.0295025
GTCTTAG4150.010.9879341
GTCTAGG3750.010.8933151
AATCGCC3400.010.34356718
AAATCGC3250.010.2354117
GGACCGT1409.741598E-810.1759236
CCGTACA10000.010.16961117
TATACCG750.002654795510.1308035
CCCCGTA10200.010.06307415
GACCGTT856.614735E-410.0561037
CCCGTAC10300.09.96568316
TGTCCCG9950.09.73525511
GTTACGG800.00450149239.50430917
TGGTTCG1106.86144E-59.4976275
CGCAGAA2002.6739144E-109.4975292
CCGTCTG1401.0643453E-69.4971369
AGCGACT8650.09.33920515
GTACTGG22800.09.3333181
CGCCCTA10450.09.27693316
Produced by FastQC (version 0.11.5)