##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294591_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4840099 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.164062140051268 31.0 30.0 34.0 26.0 34.0 2 30.138873812291855 31.0 30.0 34.0 25.0 34.0 3 30.16146281305403 31.0 30.0 34.0 25.0 34.0 4 33.766228335412144 35.0 35.0 37.0 28.0 37.0 5 34.14200866552523 37.0 35.0 37.0 30.0 37.0 6 34.21902857772124 37.0 35.0 37.0 30.0 37.0 7 34.08791948263868 37.0 35.0 37.0 29.0 37.0 8 33.9903669325772 37.0 35.0 37.0 28.0 37.0 9 35.073483827500226 39.0 35.0 39.0 27.0 39.0 10 35.171169019476665 38.0 35.0 39.0 27.0 39.0 11 35.43911891058427 39.0 35.0 39.0 28.0 39.0 12 35.213795420300286 39.0 35.0 39.0 27.0 39.0 13 35.067332093827005 38.0 35.0 39.0 27.0 39.0 14 35.96995082125386 39.0 35.0 41.0 27.0 41.0 15 35.944308163944584 39.0 35.0 41.0 27.0 41.0 16 36.0500254230337 39.0 35.0 41.0 27.0 41.0 17 36.04729056161868 39.0 35.0 41.0 27.0 41.0 18 35.91888037827326 39.0 35.0 41.0 27.0 41.0 19 36.1780385070636 39.0 35.0 41.0 27.0 41.0 20 36.319137067237676 39.0 36.0 41.0 27.0 41.0 21 36.34791953635659 39.0 36.0 41.0 27.0 41.0 22 36.35453469030282 39.0 36.0 41.0 27.0 41.0 23 36.335832593506865 39.0 36.0 41.0 27.0 41.0 24 36.39148951292112 39.0 36.0 41.0 27.0 41.0 25 35.15184916672159 38.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.021328885044717 1101 2 1.031657215674933 1101 3 1.2982023891932606 1101 4 1.1416176571109133 1101 5 0.8058757345000132 1101 6 0.8466548792439283 1101 7 0.8390822811293646 1101 8 0.9382497361732192 1101 9 1.2389853599474776 1101 10 1.1914041366500427 1101 11 0.917010069975376 1101 12 1.1347932243010277 1101 13 1.157591016484112 1101 14 1.3166013912643635 1101 15 1.4532946628945567 1101 16 1.3081827719455958 1101 17 1.2916043848664103 1101 18 1.3719290908932251 1101 19 1.3606878191129042 1101 20 1.205826745923133 1101 21 1.0667706522748404 1101 22 1.0049144405202526 1101 23 1.0402436783284799 1101 24 1.0590410745138001 1101 25 1.1261278695710502 1102 1 0.9480129766387932 1102 2 0.8912770055563861 1102 3 1.1598538150504574 1102 4 1.0363163633349828 1102 5 0.8316063736610815 1102 6 0.743612374634381 1102 7 0.658865680404837 1102 8 0.7304654179430514 1102 9 1.0246366838627594 1102 10 1.008107763418515 1102 11 0.7811266619831301 1102 12 0.9573225916997075 1102 13 0.9692505496787618 1102 14 1.2551255197348183 1102 15 1.241791403707964 1102 16 1.1750583518326039 1102 17 1.2464254620231472 1102 18 1.3496024253986434 1102 19 1.032668546919119 1102 20 0.3662552565664612 1102 21 0.5673664215374288 1102 22 0.6384410879903228 1102 23 0.7892792006552369 1102 24 0.9124609673713664 1102 25 0.8817039263689495 1103 1 0.5985222665249061 1103 2 0.5957064227067193 1103 3 0.922542111822434 1103 4 0.7755029020917235 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-1.3154952918062364 2207 10 -0.19308202985696 2207 11 0.10016171891186332 2207 12 0.4811384940232841 2207 13 0.6774283000266124 2207 14 0.8503569360348848 2207 15 0.9073427939704999 2207 16 0.8706046329823209 2207 17 0.8893948681930866 2207 18 0.9148103848873035 2207 19 -0.32799055677165256 2207 20 0.3218952669209756 2207 21 -0.32931787371538945 2207 22 0.28275632398815986 2207 23 -0.3923881739861841 2207 24 -1.5589662719333077 2207 25 -0.18414516720646645 2208 1 0.4270719890737418 2208 2 0.128451519647669 2208 3 0.6139297295598354 2208 4 0.45167736779519174 2208 5 0.3942157273174587 2208 6 0.25748542622960713 2208 7 0.30878643248838955 2208 8 0.49316422470498367 2208 9 -0.1622110395073122 2208 10 0.05063860192731795 2208 11 0.20972282891064964 2208 12 0.20285041168466478 2208 13 0.43848816934130497 2208 14 0.8144849397551681 2208 15 0.7211727739581022 2208 16 0.49729808580985235 2208 17 0.518625507233601 2208 18 0.7563342987015176 2208 19 -1.408533016021579 2208 20 -0.14087403151401645 2208 21 -0.28834568463386745 2208 22 0.13540947281564542 2208 23 0.3159865089186624 2208 24 0.4496150278787212 2208 25 0.5281000429013218 2209 1 0.12513258946896855 2209 2 -1.4207981802200322 2209 3 -0.25499187192406936 2209 4 0.10949484980256585 2209 5 0.08149917997574363 2209 6 0.15084763458285266 2209 7 0.06590884442134382 2209 8 0.21519381299409446 2209 9 -2.021867564063058 2209 10 -0.410721706642569 2209 11 -0.012072674554396201 2209 12 0.23586825062597683 2209 13 0.3879592996960781 2209 14 0.570381758740595 2209 15 0.782486567634443 2209 16 0.6932417380882683 2209 17 0.45210622957581137 2209 18 0.644536966480679 2209 19 0.4389550572658081 2209 20 0.3114008304822775 2209 21 0.42431520403531664 2209 22 0.3153064052819019 2209 23 0.2801135453994803 2209 24 0.37650496331302463 2209 25 0.4045873867274139 2210 1 0.17343472480683886 2210 2 0.22851245024250488 2210 3 0.09524951040724616 2210 4 0.2892786284805453 2210 5 0.10574903422630655 2210 6 0.12736145320380388 2210 7 0.1070485513522712 2210 8 0.24409848974417514 2210 9 0.45685314076006023 2210 10 -1.3496271204457457 2210 11 -0.41702246140702215 2210 12 -2.2997035977897013 2210 13 -0.6531182228527896 2210 14 -0.4604960633114814 2210 15 -0.7301652093330873 2210 16 -0.13333129523481801 2210 17 0.10296207497417242 2210 18 0.47564991971338344 2210 19 0.33446100638828824 2210 20 0.3207463672066879 2210 21 0.292303812731582 2210 22 0.2444708600225951 2210 23 0.15216012715477234 2210 24 0.14501154347971834 2210 25 0.027222798254001646 2211 1 -0.32802250234844266 2211 2 -0.061458719638729065 2211 3 -1.481409979929051 2211 4 -0.6111038638665391 2211 5 -0.4480379978701592 2211 6 -0.3607618480741337 2211 7 -0.43772954269748965 2211 8 0.01751805443891641 2211 9 0.157450602110913 2211 10 0.09929942675458392 2211 11 -0.19888509758153106 2211 12 -0.2552256493248848 2211 13 -0.2664476300958114 2211 14 -0.8512402665016978 2211 15 -0.3895300027989208 2211 16 -0.11322891542830149 2211 17 -0.18494452634539016 2211 18 -0.053429420860332755 2211 19 -0.06873968428205046 2211 20 -0.06754146876260592 2211 21 -0.09900959575824686 2211 22 -0.19499352427344263 2211 23 -0.5143708508225942 2211 24 -0.38133895038853893 2211 25 -0.2285743032657379 2212 1 -0.3509101687249263 2212 2 -0.38480431799210635 2212 3 -2.322181358211342 2212 4 -1.3651506867341823 2212 5 -0.6287689060701993 2212 6 -0.39367693359686484 2212 7 -0.28476713557176936 2212 8 -0.29961640699099235 2212 9 -0.894396090328577 2212 10 -0.22261694140700428 2212 11 -0.2624968036903397 2212 12 0.09766678797810613 2212 13 -0.39236220625602414 2212 14 -0.03827993326203227 2212 15 0.07636712291998293 2212 16 -1.1532726400358655 2212 17 -1.33356384747146 2212 18 -0.21697832077393286 2212 19 -0.11269325253646656 2212 20 -0.22998154222915446 2212 21 -0.0017106575815475367 2212 22 -0.292532827044532 2212 23 -0.02215567324601153 2212 24 -0.16997172458820842 2212 25 0.03305715588398073 2213 1 -3.2100730656142176 2213 2 -1.480539021574561 2213 3 -2.3877035087879044 2213 4 -1.10683085033385 2213 5 -0.7581095921402081 2213 6 -0.7242846813682036 2213 7 -0.3726530348873638 2213 8 -0.23464191523564182 2213 9 -0.28704752116357213 2213 10 -0.19415723668183915 2213 11 -0.5105023410412812 2213 12 -0.2670110151211986 2213 13 -0.6323452517675179 2213 14 -0.2858617821499223 2213 15 -0.3065944277894701 2213 16 -1.4824994506009759 2213 17 -1.064136830424772 2213 18 -0.2513686090733387 2213 19 -0.21054134728432672 2213 20 -0.1941922839944823 2213 21 -0.015897711688040772 2213 22 -0.11711074917846531 2213 23 0.005938325748452655 2213 24 0.0033887270903250055 2213 25 0.020711363796486637 2214 1 -0.7975324979208942 2214 2 -0.5759808466941045 2214 3 -6.242393518609031 2214 4 -2.0843502462540577 2214 5 -1.2090209991373584 2214 6 -0.5793152427824495 2214 7 -0.4425070446440884 2214 8 -0.3508198804913931 2214 9 -0.18851901574585384 2214 10 0.040401794729667984 2214 11 -0.547100148192996 2214 12 -0.250655857419666 2214 13 -5.082616231143152 2214 14 -3.653584729775943 2214 15 -1.981725132495562 2214 16 -2.714802289067592 2214 17 -3.0778697923799854 2214 18 -0.9143854659907902 2214 19 -0.6049593151161119 2214 20 -0.2678954283650441 2214 21 -0.20412760977372812 2214 22 -0.20981461161828463 2214 23 -0.10874631304339033 2214 24 -0.150053465113956 2214 25 0.23635708194625238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1822.0 3 231.0 4 221.0 5 548.0 6 62.0 7 117.0 8 193.0 9 481.0 10 1062.0 11 2788.0 12 5706.0 13 10387.0 14 16478.0 15 22943.0 16 28761.0 17 32385.0 18 33606.0 19 34197.0 20 34996.0 21 35192.0 22 36067.0 23 37228.0 24 39402.0 25 43198.0 26 47899.0 27 54466.0 28 64224.0 29 78247.0 30 97114.0 31 121925.0 32 159788.0 33 211098.0 34 284438.0 35 387285.0 36 543131.0 37 818736.0 38 1553677.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.288377683709996 21.211535868420555 12.943624407583917 22.556462040285535 2 14.471592579255635 22.12435406672849 40.63197792943322 22.772075424582653 3 18.726932272925914 28.616881100660564 29.015505481679572 23.640681144733954 4 12.054452907693987 17.951657499677527 38.03329634566219 31.960593246966297 5 12.961798902929496 38.87034852462683 34.29580389239473 13.87204868004894 6 30.37603430003767 38.00149932414635 18.23905495440624 13.383411421409736 7 25.201439968873586 33.59999040737441 23.912844380943834 17.285725242808166 8 23.949672080040422 37.25811199804183 22.063935916063073 16.72828000585468 9 24.989192491495096 17.42633476089615 22.093793254584792 35.49067949302396 10 15.592821150436093 30.388359891840512 32.42336928105859 21.59544967666481 11 31.35181844962763 27.673201060525404 22.135149801797517 18.83983068804945 12 21.076591953986156 28.69067817371086 31.554346120538256 18.678383751764727 13 26.486716463175757 24.638749280826964 26.653281382664556 22.221252873332723 14 20.308997422609547 24.34336703904828 28.216088977960386 27.131546560381786 15 24.59760058202291 29.45579245317736 23.993621564784057 21.95298540001567 16 21.097048681457714 31.599244184604192 26.446592628732564 20.857114505205534 17 20.525172410513782 30.39109762617423 28.74441647946393 20.339313483848052 18 20.968171764467332 29.43475553109476 29.55060178393712 20.046470920500795 19 20.849584732599055 29.213050132909135 29.888667276027377 20.048697858464433 20 22.80646443062244 27.89619466724994 28.375618713373886 20.921722188753733 21 22.414552668393714 29.50187103551029 27.9905713289262 20.09300496716979 22 21.213070985207203 28.948055575483455 28.248699937004858 21.59017350230448 23 20.54763650752926 29.587562857522677 28.748807870114334 21.115992764833734 24 21.076469048537618 29.131441217590005 28.186561834903205 21.605527898969175 25 21.907666225603705 28.006264472378433 27.16562603374132 22.920443268276546 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5102.0 1 5102.0 2 5154.5 3 5207.0 4 5207.0 5 5207.0 6 7780.0 7 10353.0 8 10353.0 9 10353.0 10 14569.0 11 18785.0 12 18785.0 13 18785.0 14 29677.5 15 40570.0 16 40570.0 17 40570.0 18 65865.5 19 91161.0 20 91161.0 21 91161.0 22 131811.5 23 172462.0 24 172462.0 25 172462.0 26 228532.0 27 284602.0 28 284602.0 29 284602.0 30 341274.0 31 397946.0 32 397946.0 33 397946.0 34 448477.5 35 499009.0 36 499009.0 37 499009.0 38 531200.5 39 563392.0 40 563392.0 41 563392.0 42 563357.0 43 563322.0 44 563322.0 45 563322.0 46 630233.5 47 697145.0 48 697145.0 49 697145.0 50 626970.5 51 556796.0 52 556796.0 53 556796.0 54 465381.0 55 373966.0 56 373966.0 57 373966.0 58 310360.0 59 246754.0 60 246754.0 61 246754.0 62 206767.0 63 166780.0 64 166780.0 65 166780.0 66 125155.5 67 83531.0 68 83531.0 69 83531.0 70 61486.0 71 39441.0 72 39441.0 73 39441.0 74 27654.0 75 15867.0 76 15867.0 77 15867.0 78 10649.5 79 5432.0 80 5432.0 81 5432.0 82 3536.0 83 1640.0 84 1640.0 85 1640.0 86 1085.0 87 530.0 88 530.0 89 530.0 90 365.5 91 201.0 92 201.0 93 201.0 94 130.0 95 59.0 96 59.0 97 59.0 98 52.5 99 46.0 100 46.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09948143622682099 2 0.05216835440762679 3 0.05605257247837286 4 0.05063946006063099 5 0.047643653569895986 6 0.06720936906455839 7 0.06301523997752939 8 0.060804541394711135 9 0.0657837783896569 10 0.06545320663895511 11 0.05937895071980966 12 0.06086652359796773 13 0.06086652359796773 14 0.06272598969566531 15 0.06561849251430601 16 0.0607218984570357 17 0.0639862944952159 18 0.14398465816504993 19 0.06892421002132394 20 0.06291193630543507 21 0.06070123772261683 22 0.06648624335989821 23 0.06545320663895511 24 0.07018451482087452 25 0.06815976284782604 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4840099.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.13144433729705 #Duplication Level Percentage of deduplicated Percentage of total 1 80.03877347109795 48.92885825273952 2 12.947941646429209 15.830527480825149 3 3.7411792260816346 6.861110688451845 4 1.4104265475339808 3.4488564792967837 5 0.6530230260757739 1.9960120384762228 6 0.34438349907551297 1.2631596422650986 7 0.1904478193584314 0.8149645187788687 8 0.1316794416272447 0.6439803564961812 9 0.08146364612089127 0.4481991313517277 >10 0.395118709872654 4.349472632899539 >50 0.03255283062427374 1.3709942914191804 >100 0.025920227291418678 3.39308786491025 >500 0.0032538235045291883 1.4090849169993176 >1k 0.003185320834985482 4.195720998948784 >5k 3.767583783313552E-4 1.6620782113571833 >10k+ 2.740060933318947E-4 3.383892494784535 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 41213 0.8514908476045635 No Hit CCCATGTACTCTGCGTTGATACCAC 40235 0.8312846493429162 No Hit GAGTACATGGGAAGCAGTGGTATCA 18777 0.38794661018297355 No Hit CATGTACTCTGCGTTGATACCACTG 14620 0.30205993720376384 No Hit GCGTTGATACCACTGCTTCCCATGT 13172 0.2721431937652515 No Hit GTATCAACGCAGAGTACATGGGAAG 11988 0.2476808842133188 No Hit GTATCAACGCAGAGTACTTTTTTTT 11453 0.23662739129922755 No Hit ACGCAGAGTACATGGGAAGCAGTGG 10157 0.2098510794923823 No Hit TATCAACGCAGAGTACATGGGAAGC 9012 0.1861945385827852 No Hit ACTCTGCGTTGATACCACTGCTTCC 8569 0.1770418332352293 No Hit GGTATCAACGCAGAGTACATGGGAA 8074 0.16681476969789252 No Hit ATTCCATTCCATTCCATTCCATTCC 8057 0.16646353721277188 No Hit GTACTGGTTCACTATCGGTCAGTCA 7983 0.16493464286577608 No Hit GCTTCCCATGTACTCTGCGTTGATA 6882 0.14218717427060892 No Hit GGTATCAACGCAGAGTACTTTTTTT 6708 0.13859220648172693 No Hit GTGGTATCAACGCAGAGTACATGGG 6571 0.13576168586634282 No Hit TATCAACGCAGAGTACTTTTTTTTT 6166 0.12739408842670366 No Hit GAATGGAATGGAATGGAATGGAATG 5980 0.12355119182479532 No Hit CAGTGGTATCAACGCAGAGTACATG 5379 0.11113409043905921 No Hit GGATACCACGTGTCCCGCCCTACTC 4881 0.10084504469846589 No Hit ATACCACTGCTTCCCATGTACTCTG 4867 0.10055579441660181 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.1321468837724184E-5 7 0.0 0.0 0.0 0.0 4.1321468837724184E-5 8 0.0 0.0 0.0 0.0 4.1321468837724184E-5 9 0.0 0.0 0.0 0.0 4.1321468837724184E-5 10 2.0660734418862092E-5 0.0 0.0 2.0660734418862092E-5 4.1321468837724184E-5 11 2.0660734418862092E-5 0.0 0.0 4.1321468837724184E-5 4.1321468837724184E-5 12 2.0660734418862092E-5 0.0 0.0 4.1321468837724184E-5 4.1321468837724184E-5 13 2.0660734418862092E-5 0.0 0.0 4.1321468837724184E-5 4.1321468837724184E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGCCG 315 0.0 11.156289 19 TATTCCG 310 0.0 11.029502 5 GTCTTAG 415 0.0 10.987934 1 GTCTAGG 375 0.0 10.893315 1 AATCGCC 340 0.0 10.343567 18 AAATCGC 325 0.0 10.23541 17 GGACCGT 140 9.741598E-8 10.175923 6 CCGTACA 1000 0.0 10.169611 17 TATACCG 75 0.0026547955 10.130803 5 CCCCGTA 1020 0.0 10.063074 15 GACCGTT 85 6.614735E-4 10.056103 7 CCCGTAC 1030 0.0 9.965683 16 TGTCCCG 995 0.0 9.735255 11 GTTACGG 80 0.0045014923 9.504309 17 TGGTTCG 110 6.86144E-5 9.497627 5 CGCAGAA 200 2.6739144E-10 9.497529 2 CCGTCTG 140 1.0643453E-6 9.497136 9 AGCGACT 865 0.0 9.339205 15 GTACTGG 2280 0.0 9.333318 1 CGCCCTA 1045 0.0 9.276933 16 >>END_MODULE