FastQCFastQC Report
Wed 25 May 2016
SRR1294591_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294591_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4840099
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC455470.9410344705759117No Hit
GTACATGGGAAGCAGTGGTATCAAC387010.7995910827443818No Hit
GTATCAACGCAGAGTACTTTTTTTT312650.6459578616057233No Hit
GGTATCAACGCAGAGTACTTTTTTT205090.4237310021964427No Hit
GAGTACATGGGAAGCAGTGGTATCA191470.39559108191795256No Hit
TATCAACGCAGAGTACTTTTTTTTT186750.3858392152722496No Hit
CATGTACTCTGCGTTGATACCACTG169020.3492077331476071No Hit
GCGTTGATACCACTGCTTCCCATGT146760.3032169383312201No Hit
ACGCAGAGTACTTTTTTTTTTTTTT120410.24877590313751846No Hit
ACGCAGAGTACATGGGAAGCAGTGG118230.24427186303420653No Hit
GTATCAACGCAGAGTACATGGGAAG116670.24104878846486405No Hit
ACTCTGCGTTGATACCACTGCTTCC97470.20138017838064884No Hit
TATCAACGCAGAGTACATGGGAAGC93600.19338447416054919No Hit
GGTATCAACGCAGAGTACATGGGAA80370.16605032252439467No Hit
ATTCCATTCCATTCCATTCCATTCC78010.1611743892015432No Hit
GTACTGGTTCACTATCGGTCAGTCA75740.1564844024884615No Hit
GCTTCCCATGTACTCTGCGTTGATA75130.1552240976889109No Hit
GTGGTATCAACGCAGAGTACATGGG69830.14427390844691398No Hit
GAATGGAATGGAATGGAATGGAATG67330.1391087248421985No Hit
CAGTGGTATCAACGCAGAGTACATG65230.13476997061423743No Hit
GTACTTTTTTTTTTTTTTTTTTTTT61570.1272081418169339No Hit
GAGTACTTTTTTTTTTTTTTTTTTT58700.12127851103872049No Hit
ATACCACTGCTTCCCATGTACTCTG54300.11218778789442117No Hit
GGATACCACGTGTCCCGCCCTACTC52280.10801431954181102No Hit
CATGGGAAGCAGTGGTATCAACGCA50020.10334499356314819No Hit
GCAGTGGTATCAACGCAGAGTACAT48480.10016324046264342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAGT500.001521648713.27513814
GACCGTT1001.4018224E-712.3787067
ATCGCCG1800.012.11594719
AATCGCC1950.011.18383618
AGGACCG1803.6379788E-1211.1090965
AAATCGC2000.010.90423917
CCGTACA10300.010.86281317
TAGACGT803.693673E-410.7123424
CCCCGTA10300.010.67880815
CCCGTAC10150.010.64967516
GGACCGT2001.8189894E-1210.474296
GAAATCG2250.010.11407816
CGTTCTA951.6083602E-410.0226210
TGGACCG1159.996093E-69.9360865
TGTCCCG10250.09.81170211
TACTGGT20200.09.7331392
CCCCGGT7950.09.66095219
GTCTTAG4150.09.6213331
GCGCCTT8250.09.53970913
CTGGTCG900.00109161399.5214899
Produced by FastQC (version 0.11.5)