FastQCFastQC Report
Wed 25 May 2016
SRR1294590_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294590_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3209788
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC249420.7770606656888243No Hit
CCCATGTACTCTGCGTTGATACCAC230340.7176174875100786No Hit
GAGTACATGGGAAGCAGTGGTATCA113540.35373052675129946No Hit
GTATCAACGCAGAGTACTTTTTTTT106160.33073835405952046No Hit
CATGTACTCTGCGTTGATACCACTG85360.26593656652713515No Hit
GCGTTGATACCACTGCTTCCCATGT76950.23973545916428124No Hit
GTATCAACGCAGAGTACATGGGAAG71960.22418926109761764No Hit
GGTATCAACGCAGAGTACTTTTTTT70240.21883065174397812No Hit
ACGCAGAGTACATGGGAAGCAGTGG61930.1929410914365684No Hit
TATCAACGCAGAGTACTTTTTTTTT59660.18586897327798596No Hit
GGTATCAACGCAGAGTACATGGGAA56310.1754321469206066No Hit
TATCAACGCAGAGTACATGGGAAGC55110.17169358225527667No Hit
GCTTCCCATGTACTCTGCGTTGATA50620.15770511946583388No Hit
ACTCTGCGTTGATACCACTGCTTCC50270.15661470477177933No Hit
GTGGTATCAACGCAGAGTACATGGG47080.1466763537031106No Hit
ATTCCATTCCATTCCATTCCATTCC38560.12013254457926817No Hit
CAGTGGTATCAACGCAGAGTACATG32540.10137741184152972No Hit
ACGCAGAGTACTTTTTTTTTTTTTT32140.10013122361975307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGC655.426137E-513.16154817
GAACGAT604.0745072E-412.67388416
TATTCCG1251.4206307E-912.1555515
GTACTAG1803.6379788E-1211.0830781
TGTCCCG3000.010.76205611
GGAACGA1355.6470526E-810.5615715
CCCGTAC3250.010.23659916
CTACACG750.002656937610.1296264
CCGTGTC750.00265728110.1294689
CGCCCTA3100.010.11866716
CTTAGAC2002.5465852E-119.9715063
CCCCGTA3150.09.95805315
ACGTGTC3250.09.934678
TGGCGCG1451.6058038E-79.83303314
CGTACCA1951.6552804E-109.7400255
CCGTACA3450.09.64332317
CGTGTCC3500.09.4963769
ACTCGGA900.00111856679.49593211
TCAGTAC3150.09.3459323
CCGTACC1856.7484507E-99.23986154
Produced by FastQC (version 0.11.5)