##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294590_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3209788 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.620552198462953 31.0 30.0 33.0 25.0 34.0 2 29.841302603162575 31.0 30.0 34.0 25.0 34.0 3 29.79787294363366 31.0 30.0 34.0 25.0 34.0 4 33.37694639022889 35.0 33.0 37.0 26.0 37.0 5 33.74903513876929 35.0 35.0 37.0 28.0 37.0 6 33.8016177392401 36.0 35.0 37.0 28.0 37.0 7 33.686464651247995 36.0 35.0 37.0 28.0 37.0 8 33.549137824678766 36.0 35.0 37.0 27.0 37.0 9 34.621579680651806 38.0 34.0 39.0 25.0 39.0 10 34.754197473478 38.0 34.0 39.0 27.0 39.0 11 34.97297142365789 38.0 35.0 39.0 27.0 39.0 12 34.77218464272407 38.0 34.0 39.0 27.0 39.0 13 34.59512154696821 38.0 34.0 39.0 25.0 39.0 14 35.44791120161207 39.0 34.0 41.0 25.0 41.0 15 35.42763571924376 39.0 34.0 41.0 25.0 41.0 16 35.53932128850877 39.0 34.0 41.0 26.0 41.0 17 35.539083266558414 39.0 34.0 41.0 26.0 41.0 18 35.43450502026925 39.0 34.0 41.0 25.0 41.0 19 35.71330256079218 39.0 34.0 41.0 26.0 41.0 20 35.845270778007766 39.0 34.0 41.0 27.0 41.0 21 35.890444166406006 39.0 34.0 41.0 27.0 41.0 22 35.906503482472985 39.0 34.0 41.0 27.0 41.0 23 35.89596851879314 39.0 34.0 41.0 27.0 41.0 24 35.91471118964866 39.0 34.0 41.0 27.0 41.0 25 34.62107590906315 38.0 32.0 40.0 23.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0844522948810287 1101 2 1.1055324034600957 1101 3 1.4016333108454582 1101 4 1.138661072950697 1101 5 0.8756021060915415 1101 6 0.9164005798524002 1101 7 0.8294339338665466 1101 8 1.0269172877115267 1101 9 1.3496967345188082 1101 10 1.3004945422040919 1101 11 0.9745396056528719 1101 12 1.2146270465996736 1101 13 1.3062229721895307 1101 14 1.3433727751718294 1101 15 1.5879019221436792 1101 16 1.584888048477545 1101 17 1.3929904772506987 1101 18 1.4665697198313978 1101 19 1.299468732485181 1101 20 1.238126995960208 1101 21 1.1947755183479245 1101 22 1.175450997118908 1101 23 1.1598817126097245 1101 24 1.2816823178761396 1101 25 1.2476968881403607 1102 1 0.8133647517188649 1102 2 0.8004942522371792 1102 3 1.1052447133716612 1102 4 1.0753666620050453 1102 5 0.894748800244173 1102 6 0.7300508084217157 1102 7 0.7479050485004919 1102 8 0.7769339071489441 1102 9 1.2086423885910307 1102 10 0.9796388506040259 1102 11 0.8450808751329504 1102 12 1.0621259866053379 1102 13 1.161220450493019 1102 14 1.2897109509626148 1102 15 1.3518109950828006 1102 16 1.2934922386333056 1102 17 1.2095384155709112 1102 18 1.2559042050538096 1102 19 1.0391775530831922 1102 20 0.29462642070967604 1102 21 0.6406419568575998 1102 22 0.6540512853197953 1102 23 0.7803365046781465 1102 24 0.8503443784561284 1102 25 0.913794240077344 1103 1 0.5819999543031962 1103 2 0.612142114453782 1103 3 0.8430226628569599 1103 4 0.683421123337915 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0.41470859692201856 2207 9 -1.0907227615502464 2207 10 -0.027445632277036225 2207 11 0.2956284310225854 2207 12 0.5578091379997474 2207 13 0.6623937017271899 2207 14 0.9436669571210174 2207 15 0.8953364830377311 2207 16 0.9306040352997087 2207 17 0.9535108563769512 2207 18 0.8555260922170547 2207 19 -0.3381636233419272 2207 20 0.2752201066552473 2207 21 -0.2570400428554436 2207 22 0.3258784490580666 2207 23 -0.5145535855964738 2207 24 -1.5051888369715698 2207 25 -0.12064301333651883 2208 1 0.4941672637076202 2208 2 0.12872256820232408 2208 3 0.6700807647294482 2208 4 0.5577316418927936 2208 5 0.41716257604859663 2208 6 0.3009611702320001 2208 7 0.37841422598375374 2208 8 0.4983312486721019 2208 9 -0.16321113956922062 2208 10 0.026843632441412524 2208 11 0.25332116432507945 2208 12 0.23002467093593992 2208 13 0.5901064100449176 2208 14 0.9006262004868617 2208 15 0.8375862185296867 2208 16 0.5335031225164144 2208 17 0.5292237671924838 2208 18 0.7058600818490177 2208 19 -1.5706860495967874 2208 20 -0.10621084517097756 2208 21 -0.2454974801140608 2208 22 0.1424230650066889 2208 23 0.33653374854258544 2208 24 0.4956343278733044 2208 25 0.5526742130157487 2209 1 0.1686127157456987 2209 2 -1.390696013124412 2209 3 -0.1504935079226044 2209 4 0.04979370780334591 2209 5 0.18794558774133208 2209 6 0.22158078459759167 2209 7 0.3034698061487404 2209 8 0.3134697393989896 2209 9 -2.0715195384425726 2209 10 -0.2986837522819812 2209 11 -0.030133498140337167 2209 12 0.3395332670561899 2209 13 0.5285357062245097 2209 14 0.7012135532830044 2209 15 0.8669287845781426 2209 16 0.7694367439193712 2209 17 0.5306728214938872 2209 18 0.6919222062650121 2209 19 0.48079873481067636 2209 20 0.3540211288860391 2209 21 0.44713271537767696 2209 22 0.3926246998157481 2209 23 0.3396742368122503 2209 24 0.5171497022663161 2209 25 0.4939963940984313 2210 1 0.2362632054496352 2210 2 0.3622215490652074 2210 3 0.24685863784835504 2210 4 0.3653384939425379 2210 5 0.31099847008275106 2210 6 0.2751476390434675 2210 7 0.18533070251384487 2210 8 0.37293818902735865 2210 9 0.500412722066919 2210 10 -1.3065935345652804 2210 11 -0.3455191631033543 2210 12 -2.2009216131854146 2210 13 -0.6662968360121866 2210 14 -0.30852325153264104 2210 15 -0.5875910743285786 2210 16 0.08235685788712033 2210 17 0.16238656726670797 2210 18 0.4242890982381766 2210 19 0.36229803195249133 2210 20 0.4204757280622289 2210 21 0.44729490219119583 2210 22 0.23244013676931274 2210 23 0.2458041295207778 2210 24 0.3404744920414515 2210 25 0.2740223653993965 2211 1 -0.3369574960759003 2211 2 -0.07423683614771193 2211 3 -1.4430000367270992 2211 4 -0.5695387309603674 2211 5 -0.45716841007325826 2211 6 -0.49788692241532573 2211 7 -0.3534825611126493 2211 8 -0.004867023163726003 2211 9 0.21919009960322455 2211 10 0.20647447764166316 2211 11 -0.12143518356395333 2211 12 -0.12893887719253883 2211 13 -0.11640468169640883 2211 14 -0.7649512860884045 2211 15 -0.17301675337431277 2211 16 -0.026851665271564684 2211 17 -0.1646544994130963 2211 18 0.13558530592812446 2211 19 0.12401570809362994 2211 20 0.033360561545201506 2211 21 -0.1257101700919776 2211 22 -0.058545339086890635 2211 23 -0.35338666803406227 2211 24 -0.1723682613819335 2211 25 -0.05212191142775424 2212 1 -0.3504829266472207 2212 2 -0.37312450271202025 2212 3 -2.37241266976136 2212 4 -1.4385950744455478 2212 5 -0.6928365511173311 2212 6 -0.49944542497066635 2212 7 -0.39913342262694584 2212 8 -0.23687966911291625 2212 9 -1.076224239781702 2212 10 -0.2438968731348865 2212 11 -0.36391403536138256 2212 12 -0.0709872389704671 2212 13 -0.3649115087250365 2212 14 -0.09959543090846523 2212 15 -0.033552667340416065 2212 16 -1.2801959011930109 2212 17 -1.4711122097441631 2212 18 -0.2962775058943379 2212 19 -0.09233885930954955 2212 20 -0.2700409437413285 2212 21 -0.13714604117864582 2212 22 -0.3858829651642779 2212 23 -0.04974399495886672 2212 24 -0.23055067272997576 2212 25 0.024652602671125123 2213 1 -3.11807457132187 2213 2 -1.4107595504371915 2213 3 -2.3445274127955607 2213 4 -0.9413597041570512 2213 5 -0.7998170238885933 2213 6 -0.6059213204253169 2213 7 -0.33734840027289437 2213 8 -0.11222770252313552 2213 9 -0.20082558164315856 2213 10 -0.08403542346037796 2213 11 -0.48477412630158057 2213 12 -0.1692045001411273 2213 13 -0.594696222815621 2213 14 -0.22285077968779632 2213 15 -0.2758289480996581 2213 16 -1.6281541938389452 2213 17 -0.8560937238323731 2213 18 -0.29556746083492413 2213 19 -0.1404169194862348 2213 20 -0.058870326840818166 2213 21 0.15192016792541807 2213 22 0.11256628529285706 2213 23 0.1402386114112062 2213 24 0.16890054531892673 2213 25 0.10798783275664903 2214 1 -0.8488022625143472 2214 2 -0.633181647866202 2214 3 -6.273528500346593 2214 4 -2.147497514426469 2214 5 -1.3447539556086454 2214 6 -0.6187792576766 2214 7 -0.5182809097745533 2214 8 -0.3406832142008227 2214 9 -0.10751914103386895 2214 10 -0.1853288502042716 2214 11 -0.7888912890702784 2214 12 -0.35347944566954936 2214 13 -5.0432123436217715 2214 14 -3.649886426563633 2214 15 -2.03598759897654 2214 16 -2.8664077147538194 2214 17 -3.2950834302934737 2214 18 -1.09880636402562 2214 19 -0.711358276014245 2214 20 -0.28408334694580617 2214 21 -0.23601703588278156 2214 22 -0.2521223340919647 2214 23 -0.09945291351488805 2214 24 -0.066551078143398 2214 25 0.03023659653747046 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1231.0 3 141.0 4 163.0 5 298.0 6 53.0 7 50.0 8 93.0 9 309.0 10 831.0 11 2218.0 12 4824.0 13 8837.0 14 13933.0 15 19576.0 16 24129.0 17 26805.0 18 28654.0 19 28840.0 20 29229.0 21 28865.0 22 28833.0 23 29077.0 24 30859.0 25 32417.0 26 35942.0 27 40163.0 28 46666.0 29 55877.0 30 68859.0 31 85155.0 32 109998.0 33 144079.0 34 190264.0 35 256415.0 36 354358.0 37 523498.0 38 958249.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.647842676201066 21.284087524772854 13.18360212496901 21.884467674057074 2 14.407209184166478 22.083674913423085 41.14802080963053 22.361095092779905 3 18.63530167220744 29.06642228030554 28.947252757529142 23.35102328995787 4 11.65494401022388 18.210166215995073 38.547554294887284 31.587335478893763 5 12.279156954798747 39.81539606654544 34.42726525228207 13.478181726373744 6 29.53500233190925 38.720293097299226 18.255357779723113 13.489346791068414 7 24.667948547689903 34.63772209243385 23.352118875760418 17.342210484115835 8 24.036063603218068 37.59217902869427 21.589594150419217 16.782163217668447 9 24.9282670746808 17.79849438793515 22.062223402703218 35.21101513468083 10 14.876348155510028 31.58749091253715 32.74233130364409 20.793829628308742 11 30.45387948502135 28.061784968359362 22.21796190654322 19.26637364007606 12 20.565774116802082 30.07626824876679 31.235566827769546 18.122390806661578 13 26.117074055751065 25.40825414519991 26.484639169890773 21.990032629158254 14 20.044684719284945 25.1505389349986 28.47916708231914 26.32560926339731 15 23.916360217326517 30.419726483832072 24.38451380237852 21.279399496462894 16 20.343422321297478 32.35902636221289 26.832865372128133 20.46468594436151 17 19.546831316978036 31.245495310134647 28.872381045013228 20.335292327874086 18 19.82228278825464 30.219413555663916 30.18431562307254 19.773988033008905 19 20.147959939207357 29.77208994193523 30.77192626943611 19.308023849421303 20 22.296852437920382 28.55107649565935 29.324780473693007 19.827290592727266 21 22.109634007771547 29.569478670326433 28.68966957671092 19.6312177451911 22 20.738925439690345 29.21992373380154 29.224849363035087 20.81630146347303 23 20.03836373345903 29.834552112920186 29.44767259705267 20.679411556568112 24 20.29073708141773 29.665707852445287 29.34850724909694 20.695047817040038 25 21.06368909870811 28.620579250281125 28.131807013841065 22.183924637169696 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4058.0 1 4058.0 2 4391.5 3 4725.0 4 4725.0 5 4725.0 6 7106.0 7 9487.0 8 9487.0 9 9487.0 10 13724.0 11 17961.0 12 17961.0 13 17961.0 14 27400.0 15 36839.0 16 36839.0 17 36839.0 18 55937.0 19 75035.0 20 75035.0 21 75035.0 22 104431.0 23 133827.0 24 133827.0 25 133827.0 26 170609.0 27 207391.0 28 207391.0 29 207391.0 30 244854.0 31 282317.0 32 282317.0 33 282317.0 34 309229.5 35 336142.0 36 336142.0 37 336142.0 38 351049.5 39 365957.0 40 365957.0 41 365957.0 42 365812.5 43 365668.0 44 365668.0 45 365668.0 46 401202.5 47 436737.0 48 436737.0 49 436737.0 50 395845.0 51 354953.0 52 354953.0 53 354953.0 54 297332.5 55 239712.0 56 239712.0 57 239712.0 58 199413.5 59 159115.0 60 159115.0 61 159115.0 62 129671.0 63 100227.0 64 100227.0 65 100227.0 66 74259.0 67 48291.0 68 48291.0 69 48291.0 70 34399.0 71 20507.0 72 20507.0 73 20507.0 74 14026.0 75 7545.0 76 7545.0 77 7545.0 78 4953.0 79 2361.0 80 2361.0 81 2361.0 82 1521.5 83 682.0 84 682.0 85 682.0 86 419.0 87 156.0 88 156.0 89 156.0 90 107.0 91 58.0 92 58.0 93 58.0 94 36.0 95 14.0 96 14.0 97 14.0 98 18.5 99 23.0 100 23.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09823078658154372 2 0.05165450179264176 3 0.054614198819361276 4 0.05025254004314304 5 0.04716822419424585 6 0.0659233569319843 7 0.06237172049992087 8 0.059193940534390435 9 0.06405407459931933 10 0.06455254988802998 11 0.05882008406785744 12 0.059193940534390435 13 0.05984818935082317 14 0.06168631697794372 15 0.06480178753238532 16 0.059225095239934855 17 0.06293250519972035 18 0.13929268848908402 19 0.06738762809257184 20 0.06143707933358839 21 0.06022204581735616 22 0.06467716871020765 23 0.06452139518248558 24 0.0733381768515553 25 0.06726300927039419 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3209788.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.248479917444 #Duplication Level Percentage of deduplicated Percentage of total 1 88.18525866650357 64.59436148447992 2 8.745461422593447 12.811835107632344 3 1.7234572131782169 3.7872186320417582 4 0.5226560914399072 1.5313505687026325 5 0.22914849514011798 0.8392389472191721 6 0.13546669639361614 0.5953637754162165 7 0.0818270890226885 0.41955969208869104 8 0.05201890763289933 0.3048244728860641 9 0.04168473968767908 0.2748009436089157 >10 0.23733352237369723 3.2840861613423673 >50 0.023594483045253207 1.1975113415925376 >100 0.017661769724920222 2.632810119287336 >500 0.0021509239154518345 1.104407359074614 >1k 0.0016777206528340632 2.406951995308666 >5k 4.3018478277796496E-4 2.0116542603683993 >10k+ 1.72073913111186E-4 2.2040251389503305 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 24942 0.7770606656888243 No Hit CCCATGTACTCTGCGTTGATACCAC 23034 0.7176174875100786 No Hit GAGTACATGGGAAGCAGTGGTATCA 11354 0.35373052675129946 No Hit GTATCAACGCAGAGTACTTTTTTTT 10616 0.33073835405952046 No Hit CATGTACTCTGCGTTGATACCACTG 8536 0.26593656652713515 No Hit GCGTTGATACCACTGCTTCCCATGT 7695 0.23973545916428124 No Hit GTATCAACGCAGAGTACATGGGAAG 7196 0.22418926109761764 No Hit GGTATCAACGCAGAGTACTTTTTTT 7024 0.21883065174397812 No Hit ACGCAGAGTACATGGGAAGCAGTGG 6193 0.1929410914365684 No Hit TATCAACGCAGAGTACTTTTTTTTT 5966 0.18586897327798596 No Hit GGTATCAACGCAGAGTACATGGGAA 5631 0.1754321469206066 No Hit TATCAACGCAGAGTACATGGGAAGC 5511 0.17169358225527667 No Hit GCTTCCCATGTACTCTGCGTTGATA 5062 0.15770511946583388 No Hit ACTCTGCGTTGATACCACTGCTTCC 5027 0.15661470477177933 No Hit GTGGTATCAACGCAGAGTACATGGG 4708 0.1466763537031106 No Hit ATTCCATTCCATTCCATTCCATTCC 3856 0.12013254457926817 No Hit CAGTGGTATCAACGCAGAGTACATG 3254 0.10137741184152972 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 3214 0.10013122361975307 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.1154705544416016E-5 0.0 9 0.0 0.0 0.0 6.230941108883203E-5 0.0 10 0.0 0.0 0.0 6.230941108883203E-5 0.0 11 0.0 0.0 0.0 6.230941108883203E-5 0.0 12 0.0 0.0 0.0 6.230941108883203E-5 0.0 13 0.0 0.0 0.0 6.230941108883203E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAATCGC 65 5.426137E-5 13.161548 17 GAACGAT 60 4.0745072E-4 12.673884 16 TATTCCG 125 1.4206307E-9 12.155551 5 GTACTAG 180 3.6379788E-12 11.083078 1 TGTCCCG 300 0.0 10.762056 11 GGAACGA 135 5.6470526E-8 10.56157 15 CCCGTAC 325 0.0 10.236599 16 CTACACG 75 0.0026569376 10.129626 4 CCGTGTC 75 0.002657281 10.129468 9 CGCCCTA 310 0.0 10.118667 16 CTTAGAC 200 2.5465852E-11 9.971506 3 CCCCGTA 315 0.0 9.958053 15 ACGTGTC 325 0.0 9.93467 8 TGGCGCG 145 1.6058038E-7 9.833033 14 CGTACCA 195 1.6552804E-10 9.740025 5 CCGTACA 345 0.0 9.643323 17 CGTGTCC 350 0.0 9.496376 9 ACTCGGA 90 0.0011185667 9.495932 11 TCAGTAC 315 0.0 9.345932 3 CCGTACC 185 6.7484507E-9 9.2398615 4 >>END_MODULE