FastQCFastQC Report
Wed 25 May 2016
SRR1294590_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294590_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3209788
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT282010.8785938510580762No Hit
CCCATGTACTCTGCGTTGATACCAC266900.8315190909804635No Hit
GTACATGGGAAGCAGTGGTATCAAC246770.768804668719554No Hit
GGTATCAACGCAGAGTACTTTTTTT212430.6618194098800294No Hit
TATCAACGCAGAGTACTTTTTTTTT176480.5498182434478539No Hit
GAGTACATGGGAAGCAGTGGTATCA122950.38304710466859493No Hit
ACGCAGAGTACTTTTTTTTTTTTTT109970.34260829687194294No Hit
CATGTACTCTGCGTTGATACCACTG100050.3117028289718823No Hit
GCGTTGATACCACTGCTTCCCATGT82580.2572755583857875No Hit
ACGCAGAGTACATGGGAAGCAGTGG74810.23306835217777622No Hit
GTATCAACGCAGAGTACATGGGAAG73920.2302955833843232No Hit
GGTATCAACGCAGAGTACATGGGAA62910.19599425257992115No Hit
TATCAACGCAGAGTACATGGGAAGC58420.18200578979047838No Hit
ACTCTGCGTTGATACCACTGCTTCC56940.1773948933699048No Hit
GCTTCCCATGTACTCTGCGTTGATA56420.17577484868159515No Hit
GTGGTATCAACGCAGAGTACATGGG50040.15589814654425777No Hit
GTACTTTTTTTTTTTTTTTTTTTTT49970.15568006360544684No Hit
GAGTACTTTTTTTTTTTTTTTTTTT49440.1540288642115928No Hit
CAGTGGTATCAACGCAGAGTACATG39620.12343494336697627No Hit
ATTCCATTCCATTCCATTCCATTCC38650.12041293692916791No Hit
GAATGGAATGGAATGGAATGGAATG32490.10122163831380764No Hit
CATGGGAAGCAGTGGTATCAACGCA32370.10084778184727464No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGAC604.1045292E-412.662863
CTACGCT550.003017760812.1184724
GGTGCGC550.003017760812.1184728
TACGACA657.875605E-411.7189624
CGGTTTT658.151566E-411.6709514
AATTCCG600.005779177411.10865
TCTAGAC2950.010.6239243
CGCCCTA2950.010.60737516
TTACACC2300.010.34962754
TAGACAG4750.010.2232525
AATCGCC750.00269005810.11450818
CTAAGAC3400.09.7764733
GTCCCGC3150.09.63316412
TGAGCGG800.00443759379.52061510
AAATCGC800.0045874839.48235117
GTCTTGG4350.09.3988331
AGAGGCG2850.09.31628319
CCCCGGT2550.09.29671219
CCGGCTA1651.031749E-79.2321110
CGGCTAA1651.0869189E-79.20088311
Produced by FastQC (version 0.11.5)