##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294590_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3209788 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.21559928568491 31.0 31.0 34.0 28.0 34.0 2 31.427040664367865 31.0 31.0 34.0 28.0 34.0 3 31.49334410870749 31.0 31.0 34.0 28.0 34.0 4 35.09135494306789 37.0 35.0 37.0 32.0 37.0 5 35.30694955554697 37.0 35.0 37.0 32.0 37.0 6 34.85383427192076 37.0 35.0 37.0 32.0 37.0 7 34.708895416145864 37.0 35.0 37.0 32.0 37.0 8 34.73382603461662 37.0 35.0 37.0 32.0 37.0 9 36.18573313876181 39.0 37.0 39.0 31.0 39.0 10 35.96468209115368 39.0 35.0 39.0 30.0 39.0 11 36.130683085611885 39.0 35.0 39.0 30.0 39.0 12 36.10326756782691 39.0 35.0 39.0 30.0 39.0 13 35.945589241407845 39.0 35.0 39.0 30.0 39.0 14 37.02089172244398 40.0 36.0 41.0 30.0 41.0 15 37.05161306603427 40.0 36.0 41.0 30.0 41.0 16 37.08161286664415 40.0 36.0 41.0 31.0 41.0 17 37.1595086653698 40.0 37.0 41.0 31.0 41.0 18 37.181121930794184 40.0 37.0 41.0 31.0 41.0 19 37.213662397641215 40.0 37.0 41.0 30.0 41.0 20 37.292694720025125 40.0 37.0 41.0 31.0 41.0 21 37.14413226044835 40.0 37.0 41.0 30.0 41.0 22 37.14886497176761 40.0 37.0 41.0 30.0 41.0 23 37.16385381215208 40.0 37.0 41.0 30.0 41.0 24 37.09845603510263 40.0 36.0 41.0 30.0 41.0 25 36.04010856791788 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4795364404023523 1101 2 0.5112675894306911 1101 3 0.44325850468111483 1101 4 0.356889987766003 1101 5 0.3978368474166487 1101 6 0.48820675914521416 1101 7 0.45948478084761746 1101 8 0.4400686104255911 1101 9 0.6552655874958546 1101 10 0.7261747601842146 1101 11 0.6164854397664286 1101 12 0.7570435295225693 1101 13 0.7491688360407522 1101 14 0.9844961387208642 1101 15 0.9999207589503172 1101 16 0.9438730048950319 1101 17 0.965582692604741 1101 18 0.9554186339221502 1101 19 1.1272578719182889 1101 20 0.9887738630509375 1101 21 1.2547894755140163 1101 22 1.2091694016510104 1101 23 1.161058371665682 1101 24 1.252646247795468 1101 25 1.276314929360467 1102 1 0.3500186049413969 1102 2 0.38177490969888694 1102 3 0.30171975787732563 1102 4 0.3180386419589851 1102 5 0.3536933926458943 1102 6 0.43431387944128375 1102 7 0.4458971354214114 1102 8 0.4921220356095901 1102 9 0.5489324918396647 1102 10 0.7117177846866838 1102 11 0.5872752782235366 1102 12 0.6437067555452032 1102 13 0.7276898849195348 1102 14 0.9089359461068511 1102 15 0.8088845266171987 1102 16 0.7460294901865225 1102 17 0.7280854206664102 1102 18 0.705745352221335 1102 19 0.8810686464575568 1102 20 0.8286356265630843 1102 21 1.1739135858474867 1102 22 0.9947167861532691 1102 23 0.9578870351861326 1102 24 1.0594402052276664 1102 25 0.9997844163428056 1103 1 0.21244113224165417 1103 2 0.21030207523203615 1103 3 0.16959656631661701 1103 4 0.12295561064102145 1103 5 0.14021303144591002 1103 6 0.1709247543720025 1103 7 0.21053899893671968 1103 8 0.22384256477914732 1103 9 0.11129592863833437 1103 10 0.43969037620389884 1103 11 0.4827887340668795 1103 12 0.4083916367475382 1103 13 0.4728490454084451 1103 14 0.6392219084721944 1103 15 0.49593867937183944 1103 16 0.5095283759863278 1103 17 0.5118367214747082 1103 18 0.5301344738234448 1103 19 0.6729871164246717 1103 20 0.628369323057818 1103 21 0.8019909849936724 1103 22 0.6886725906186868 1103 23 0.7133384187607561 1103 24 0.8772080454326883 1103 25 0.8311094898839784 1104 1 0.16132245873680517 1104 2 0.2117006790379392 1104 3 0.07199648633225664 1104 4 0.09549892777093305 1104 5 0.12043456298480493 1104 6 0.1306874863887657 1104 7 0.1865072452646075 1104 8 0.12445383748541161 1104 9 -0.04450347644803543 1104 10 0.3745082097232739 1104 11 0.438488936991277 1104 12 0.4347346900368194 1104 13 -0.017546395243428492 1104 14 0.34563177803961054 1104 15 0.5265697416222395 1104 16 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0.24866396171719884 2207 4 0.16713959137148038 2207 5 0.14406750551549408 2207 6 0.2601161313068445 2207 7 0.2448905736395517 2207 8 0.33402123257270233 2207 9 0.36368140452974984 2207 10 0.5631701157544953 2207 11 0.4315793346636525 2207 12 0.41351376679197216 2207 13 0.4744049602063569 2207 14 0.44046749874627267 2207 15 0.46347156624054264 2207 16 0.5838033587103126 2207 17 0.5669400049524356 2207 18 0.5409582755934679 2207 19 0.5696055038843326 2207 20 0.43877793457311043 2207 21 0.8755367850772515 2207 22 0.7944253392714273 2207 23 0.731152423827865 2207 24 0.8359599149691803 2207 25 0.7196328282157509 2208 1 0.17912228993372636 2208 2 0.14787431328311484 2208 3 0.1806056935401017 2208 4 0.1438309843493215 2208 5 0.09237730757578078 2208 6 0.21836366341197078 2208 7 0.22117301892468788 2208 8 0.23865003867503987 2208 9 0.27147405054741114 2208 10 0.30709913387729415 2208 11 0.028493351011768198 2208 12 -0.16214569664908396 2208 13 -0.008827525710074724 2208 14 -0.46758852614664903 2208 15 0.07051169580902439 2208 16 0.24697781658643692 2208 17 0.10660793114537626 2208 18 0.2528485097418951 2208 19 0.4377410449204362 2208 20 0.365785382201274 2208 21 0.6356720257541681 2208 22 0.650755597373319 2208 23 0.5799848057101116 2208 24 0.6501707249641413 2208 25 0.5953588374019247 2209 1 0.026532797510181894 2209 2 0.09045540599751689 2209 3 0.14755743491264184 2209 4 -0.4138313473430699 2209 5 0.027743426241521263 2209 6 0.13754571561553064 2209 7 0.19000424745527766 2209 8 0.23917554286653342 2209 9 0.30446484847799127 2209 10 0.3306308563609335 2209 11 0.2787451333381128 2209 12 0.306003316698515 2209 13 0.313221919741963 2209 14 0.4045624680278763 2209 15 -0.20832626084681038 2209 16 -0.14458131151539533 2209 17 0.2283577451816683 2209 18 -0.16145356218635953 2209 19 0.2818439058362969 2209 20 0.3662555151757729 2209 21 0.6101197762201238 2209 22 0.6022681718632725 2209 23 0.6023976117144372 2209 24 0.6165008460703945 2209 25 0.6680476636992836 2210 1 -0.13920056912019163 2210 2 -0.11308996952261907 2210 3 -0.026369163802634432 2210 4 0.04214342411609806 2210 5 -0.03991411929438726 2210 6 -0.4158704846525296 2210 7 -0.09577504923495894 2210 8 -0.18569021141455266 2210 9 0.03411222816583859 2210 10 0.2523501475310823 2210 11 0.12760050221312014 2210 12 0.12086592553836795 2210 13 0.16936216749217436 2210 14 0.23147969815544656 2210 15 0.10790702851004141 2210 16 0.27915005962837114 2210 17 -0.8470552788914674 2210 18 -1.3727107187464185 2210 19 -0.3378758014819496 2210 20 -0.17872760338128302 2210 21 -0.38286516965143136 2210 22 -0.2503123843270316 2210 23 -0.15822550291859727 2210 24 0.28130941210344673 2210 25 0.35593337440355555 2211 1 -0.22840237437959843 2211 2 -0.24843265331302788 2211 3 -0.09063910324317348 2211 4 -0.0946473148745639 2211 5 -0.053300786480150464 2211 6 -0.028111174309913167 2211 7 -0.16271413182546723 2211 8 -0.08198507459275106 2211 9 -0.06908218057049709 2211 10 0.009241766837831733 2211 11 -0.2068270955963598 2211 12 -0.057046630151184274 2211 13 0.035964599251606444 2211 14 -0.033535060410727624 2211 15 -0.10272210047931907 2211 16 -0.22276016177755764 2211 17 -0.297925256166792 2211 18 -0.39253230095044245 2211 19 -0.25857409646296503 2211 20 -0.31264713417204604 2211 21 0.08562474537149711 2211 22 -0.04571010560483302 2211 23 -0.6689743488491473 2211 24 -0.11476619781150532 2211 25 -0.8935251192193618 2212 1 -0.19346597113265318 2212 2 -0.2994193448928151 2212 3 -0.2086656782726628 2212 4 -0.1475597483777591 2212 5 -0.16895081642142173 2212 6 -0.1406728860867119 2212 7 -0.23453910243005538 2212 8 -0.4047153099836649 2212 9 -0.1861595893332293 2212 10 -0.4816788727579535 2212 11 -0.2180507181235356 2212 12 -0.3753033279143452 2212 13 -0.34795094359136414 2212 14 -0.4039400968628968 2212 15 -0.25482789654324023 2212 16 -0.4570207261229555 2212 17 -0.7119312569649168 2212 18 -0.44480915018997536 2212 19 -0.47940336290871244 2212 20 -0.7136575999133328 2212 21 -0.14471308232750602 2212 22 -0.5438336657407987 2212 23 -0.3611638225284679 2212 24 -0.5890866772249623 2212 25 -0.18832303768525094 2213 1 -0.1231026705166478 2213 2 -0.24553453250923596 2213 3 -0.06748317615798882 2213 4 -0.026966388214454184 2213 5 -0.09026746386079765 2213 6 -0.3156167009881088 2213 7 -0.32740511024747576 2213 8 -0.2613521407383601 2213 9 -0.24154536151465322 2213 10 -0.28643076891226116 2213 11 -0.05117080084502135 2213 12 0.10219368655647543 2213 13 0.12433299179998158 2213 14 -0.5576448744765372 2213 15 -0.1964415036290461 2213 16 -0.2525629729380867 2213 17 -0.20116352878500976 2213 18 -0.2658986698011958 2213 19 -0.31326070496017877 2213 20 -0.33187673144657737 2213 21 -0.031771014334879055 2213 22 -0.455752174091991 2213 23 -0.510479092925749 2213 24 -0.7000579681487267 2213 25 -0.44639358424924325 2214 1 -0.19555321296347472 2214 2 -0.22318483161197378 2214 3 -0.20461387261422814 2214 4 -0.06695650105057638 2214 5 -0.26001790915618983 2214 6 -0.19056245137273464 2214 7 -0.1403401052277502 2214 8 -0.22287611547689323 2214 9 -0.25641137459843577 2214 10 -0.2872788478546866 2214 11 -0.20004383001714388 2214 12 -0.25511932806729476 2214 13 -0.24902847559916808 2214 14 -0.32706216748130856 2214 15 -0.0767226601411366 2214 16 -0.30520706481817683 2214 17 -0.45187613397561677 2214 18 -0.38424782417924774 2214 19 -0.6231020152399864 2214 20 -0.5929639532367617 2214 21 -0.6382509786779522 2214 22 -0.8571657253145162 2214 23 -1.0981617312423069 2214 24 -0.6723805681300234 2214 25 -0.997016128450305 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 15.0 9 42.0 10 98.0 11 213.0 12 519.0 13 1250.0 14 2850.0 15 5001.0 16 7552.0 17 9982.0 18 11773.0 19 13396.0 20 14423.0 21 15560.0 22 16711.0 23 18019.0 24 19829.0 25 22197.0 26 25431.0 27 28424.0 28 33676.0 29 42030.0 30 53411.0 31 70243.0 32 94252.0 33 127429.0 34 175486.0 35 246467.0 36 354229.0 37 569064.0 38 1230214.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.580673327595456 21.414511843730118 12.490177001085208 23.51463782758922 2 14.321849293473587 22.45154508646677 39.74405786301151 23.482547757048128 3 18.47652022426375 28.376302867751964 29.54547936205068 23.601697545933607 4 11.520327386868756 18.194100231381803 38.41252639890024 31.873045982849206 5 12.302564949533036 38.91057422336345 34.91036232132451 13.876498505779006 6 29.942385472040282 38.53184223154248 17.677595458143 13.848176838274243 7 24.616917339157013 34.590435305057575 23.28994866885553 17.50269868692988 8 24.5262046230126 36.95187927328887 21.286194314611286 17.23572178908724 9 25.058742307109956 16.328710174086588 22.508413343959184 36.10413417484427 10 15.100430045844657 30.78779807081478 32.984976076764546 21.12679580657602 11 31.009916349506426 26.757838804451385 22.20373160013368 20.028513245908506 12 20.994928868003825 29.337575010039764 31.600190295965056 18.06730582599135 13 26.993660326993663 24.122192531778587 26.91125610515589 21.972891036071864 14 20.515066065625405 23.985914224819716 29.202249511879845 26.29677019767503 15 24.378766655533898 29.19603557168422 25.15403097567906 21.271166797102822 16 20.283639023685083 31.42675644217804 27.618088943056605 20.671515591080272 17 19.313555696997962 30.565984596485684 29.613024166401075 20.507435540115278 18 19.573597342873335 29.091187784345546 31.398633490176753 19.936581382604366 19 19.90377512677688 28.754837180074727 32.30954338515527 19.031844307993122 20 21.89369869978709 27.36459983886756 31.052358072915855 19.689343388429496 21 21.675428424734143 28.69855335969857 30.170304400735006 19.455713814832283 22 20.28713595885471 28.34807874507859 30.733442606300464 20.631342689766235 23 19.566538080731863 28.832700996265793 31.108615963805686 20.492144959196658 24 19.857915849894137 28.766105425031185 30.959334385946985 20.416644339127693 25 20.682706462391636 27.74349827477432 29.67652992078761 21.89726534204644 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2652.0 1 2652.0 2 2900.0 3 3148.0 4 3148.0 5 3148.0 6 6347.5 7 9547.0 8 9547.0 9 9547.0 10 13027.5 11 16508.0 12 16508.0 13 16508.0 14 22756.5 15 29005.0 16 29005.0 17 29005.0 18 47788.5 19 66572.0 20 66572.0 21 66572.0 22 98339.5 23 130107.0 24 130107.0 25 130107.0 26 169883.0 27 209659.0 28 209659.0 29 209659.0 30 248551.5 31 287444.0 32 287444.0 33 287444.0 34 315097.0 35 342750.0 36 342750.0 37 342750.0 38 353224.0 39 363698.0 40 363698.0 41 363698.0 42 362365.0 43 361032.0 44 361032.0 45 361032.0 46 401359.5 47 441687.0 48 441687.0 49 441687.0 50 401925.5 51 362164.0 52 362164.0 53 362164.0 54 302561.0 55 242958.0 56 242958.0 57 242958.0 58 201761.0 59 160564.0 60 160564.0 61 160564.0 62 130966.5 63 101369.0 64 101369.0 65 101369.0 66 74977.5 67 48586.0 68 48586.0 69 48586.0 70 34454.5 71 20323.0 72 20323.0 73 20323.0 74 13727.5 75 7132.0 76 7132.0 77 7132.0 78 4632.0 79 2132.0 80 2132.0 81 2132.0 82 1341.5 83 551.0 84 551.0 85 551.0 86 351.0 87 151.0 88 151.0 89 151.0 90 94.0 91 37.0 92 37.0 93 37.0 94 23.0 95 9.0 96 9.0 97 9.0 98 6.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0943364483884917 2 0.0 3 0.13879421320037336 4 0.01165185987361159 5 0.02361526680266734 6 0.016449684527451658 7 0.025546858546421136 8 0.023521802686034092 9 1.869282332664961E-4 10 0.395259749242006 11 0.06716954515376093 12 0.0015888899827652169 13 3.1154705544416016E-5 14 7.788676386104004E-4 15 0.0013708070439543048 16 1.2461882217766406E-4 17 3.1154705544416016E-5 18 1.557735277220801E-4 19 4.6732058316624025E-4 20 1.869282332664961E-4 21 1.869282332664961E-4 22 0.0027727687934530256 23 6.230941108883203E-5 24 0.0 25 0.0019627464492982093 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3209788.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.66328450093522 #Duplication Level Percentage of deduplicated Percentage of total 1 86.89053333382375 60.530799440721594 2 9.71861794527134 13.540616937546638 3 1.945742281400455 4.066403943440959 4 0.56100701454462 1.5632636504496862 5 0.2624404608194307 0.9141232243310272 6 0.13612005088199838 0.568954189852466 7 0.08682692866810456 0.42340543239040035 8 0.0621580216407298 0.34641055564587586 9 0.04077946697989095 0.2556748449014974 >10 0.2453925964977239 3.262443047842259 >50 0.02593746839461688 1.2656815492581959 >100 0.019269167111342512 2.7198192772951866 >500 0.0024751262454974932 1.177759694700969 >1k 0.001980100995054571 2.9892925455512245 >5k 3.600183627371947E-4 1.6177893753736197 >10k+ 3.600183627371947E-4 4.7575622906983766 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 28201 0.8785938510580762 No Hit CCCATGTACTCTGCGTTGATACCAC 26690 0.8315190909804635 No Hit GTACATGGGAAGCAGTGGTATCAAC 24677 0.768804668719554 No Hit GGTATCAACGCAGAGTACTTTTTTT 21243 0.6618194098800294 No Hit TATCAACGCAGAGTACTTTTTTTTT 17648 0.5498182434478539 No Hit GAGTACATGGGAAGCAGTGGTATCA 12295 0.38304710466859493 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 10997 0.34260829687194294 No Hit CATGTACTCTGCGTTGATACCACTG 10005 0.3117028289718823 No Hit GCGTTGATACCACTGCTTCCCATGT 8258 0.2572755583857875 No Hit ACGCAGAGTACATGGGAAGCAGTGG 7481 0.23306835217777622 No Hit GTATCAACGCAGAGTACATGGGAAG 7392 0.2302955833843232 No Hit GGTATCAACGCAGAGTACATGGGAA 6291 0.19599425257992115 No Hit TATCAACGCAGAGTACATGGGAAGC 5842 0.18200578979047838 No Hit ACTCTGCGTTGATACCACTGCTTCC 5694 0.1773948933699048 No Hit GCTTCCCATGTACTCTGCGTTGATA 5642 0.17577484868159515 No Hit GTGGTATCAACGCAGAGTACATGGG 5004 0.15589814654425777 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 4997 0.15568006360544684 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 4944 0.1540288642115928 No Hit CAGTGGTATCAACGCAGAGTACATG 3962 0.12343494336697627 No Hit ATTCCATTCCATTCCATTCCATTCC 3865 0.12041293692916791 No Hit GAATGGAATGGAATGGAATGGAATG 3249 0.10122163831380764 No Hit CATGGGAAGCAGTGGTATCAACGCA 3237 0.10084778184727464 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.1154705544416016E-5 0.0 4 0.0 0.0 0.0 6.230941108883203E-5 0.0 5 0.0 0.0 0.0 6.230941108883203E-5 0.0 6 0.0 0.0 0.0 6.230941108883203E-5 0.0 7 0.0 0.0 0.0 6.230941108883203E-5 0.0 8 3.1154705544416016E-5 0.0 0.0 6.230941108883203E-5 0.0 9 3.1154705544416016E-5 0.0 0.0 9.346411663324805E-5 0.0 10 3.1154705544416016E-5 0.0 0.0 9.346411663324805E-5 0.0 11 3.1154705544416016E-5 0.0 0.0 1.2461882217766406E-4 0.0 12 3.1154705544416016E-5 0.0 0.0 1.2461882217766406E-4 0.0 13 3.1154705544416016E-5 0.0 0.0 1.2461882217766406E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGAC 60 4.1045292E-4 12.66286 3 CTACGCT 55 0.0030177608 12.118472 4 GGTGCGC 55 0.0030177608 12.118472 8 TACGACA 65 7.875605E-4 11.718962 4 CGGTTTT 65 8.151566E-4 11.67095 14 AATTCCG 60 0.0057791774 11.1086 5 TCTAGAC 295 0.0 10.623924 3 CGCCCTA 295 0.0 10.607375 16 TTACACC 230 0.0 10.3496275 4 TAGACAG 475 0.0 10.223252 5 AATCGCC 75 0.002690058 10.114508 18 CTAAGAC 340 0.0 9.776473 3 GTCCCGC 315 0.0 9.633164 12 TGAGCGG 80 0.0044375937 9.520615 10 AAATCGC 80 0.004587483 9.482351 17 GTCTTGG 435 0.0 9.398833 1 AGAGGCG 285 0.0 9.316283 19 CCCCGGT 255 0.0 9.296712 19 CCGGCTA 165 1.031749E-7 9.23211 10 CGGCTAA 165 1.0869189E-7 9.200883 11 >>END_MODULE