FastQCFastQC Report
Wed 25 May 2016
SRR1294589_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294589_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5571849
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC153270.2750792420971925No Hit
CCCATGTACTCTGCGTTGATACCAC149890.26901303319598213No Hit
GTATCAACGCAGAGTACTTTTTTTT107510.19295210620388314No Hit
GGTATCAACGCAGAGTACTTTTTTT69280.12433933511119917No Hit
GAGTACATGGGAAGCAGTGGTATCA68260.12250870402266824No Hit
GTACTGGTTCACTATCGGTCAGTCA66060.11856028402779759No Hit
ATTCCATTCCATTCCATTCCATTCC65470.11750138957462775No Hit
TATCAACGCAGAGTACTTTTTTTTT61390.110178865220504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG1002.4082148E-510.4473285
ACACCGT1856.002665E-1110.2672746
AATCGCC2053.6379788E-1210.20011818
AAATCGC2250.010.13757117
ACGGTCC1151.0311243E-59.9102418
CGCCCTA9450.09.65465616
GTACTGG21750.09.5228261
TATTCCG2650.09.3183715
TACTGGT20200.09.16852952
TATACTG12150.09.1458085
TGTCCCG9800.09.10917911
GCCGCTA1151.10682624E-49.09059216
GTCCCGC10500.09.05099512
ACCGTCC1054.5088076E-49.0450618
GTCCTAG5050.09.0306381
ATTAGAC3600.08.9700093
CCGCCCT10400.08.95550515
CGGGACT850.00745431248.9390514
TAGACAG7500.08.8643995
GTATTAT10700.08.790631
Produced by FastQC (version 0.11.5)