##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294589_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5571849
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.913399663199776	31.0	30.0	33.0	25.0	34.0
2	30.128955038085202	31.0	30.0	34.0	25.0	34.0
3	30.019436276898386	31.0	30.0	34.0	25.0	34.0
4	33.57622828615779	35.0	33.0	37.0	27.0	37.0
5	33.97527409662394	35.0	35.0	37.0	28.0	37.0
6	34.08039180530557	37.0	35.0	37.0	29.0	37.0
7	33.95068369584316	37.0	35.0	37.0	28.0	37.0
8	33.76677975300479	36.0	35.0	37.0	28.0	37.0
9	34.88843434199312	38.0	35.0	39.0	27.0	39.0
10	34.99797661422627	38.0	35.0	39.0	27.0	39.0
11	35.253944965127374	39.0	35.0	39.0	27.0	39.0
12	35.01598553729651	38.0	35.0	39.0	27.0	39.0
13	34.88260055145069	38.0	35.0	39.0	27.0	39.0
14	35.74929776453023	39.0	34.0	41.0	27.0	41.0
15	35.734312792755155	39.0	34.0	41.0	27.0	41.0
16	35.83470424270292	39.0	34.0	41.0	27.0	41.0
17	35.805231800072114	39.0	34.0	41.0	27.0	41.0
18	35.685642952635654	39.0	34.0	41.0	26.0	41.0
19	35.94636125279059	39.0	34.0	41.0	27.0	41.0
20	36.07430334167347	39.0	35.0	41.0	27.0	41.0
21	36.101627125932524	39.0	35.0	41.0	27.0	41.0
22	36.09430513999931	39.0	35.0	41.0	27.0	41.0
23	36.05383096347371	39.0	35.0	41.0	27.0	41.0
24	36.06044905380602	39.0	35.0	41.0	27.0	41.0
25	34.75425913372742	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	1.1040825812455068
1101	2	1.1226089024320416
1101	3	1.4138516190782582
1101	4	1.2177997198024855
1101	5	0.9244800115382432
1101	6	0.9055774744425236
1101	7	0.917181298014583
1101	8	1.092712151469179
1101	9	1.3934169176528712
1101	10	1.2567083273655584
1101	11	1.0833915453624456
1101	12	1.2777096433862098
1101	13	1.3316025999048904
1101	14	1.4007664084599725
1101	15	1.532992891388595
1101	16	1.4932692321945922
1101	17	1.4141713202144146
1101	18	1.441665242931073
1101	19	1.376991411424548
1101	20	1.3700433741610922
1101	21	1.266298669637294
1101	22	1.143982383532041
1101	23	1.22479180799845
1101	24	1.2717920352835108
1101	25	1.233506367483642
1102	1	0.9487106155470997
1102	2	0.9165746377032775
1102	3	1.1880750915372076
1102	4	1.0294014543680134
1102	5	0.876551184248342
1102	6	0.7343858678477844
1102	7	0.6688158701029678
1102	8	0.814943046602643
1102	9	1.2299827181527547
1102	10	1.0707648510896917
1102	11	0.8488607483979749
1102	12	1.0875589692126084
1102	13	1.085336789762401
1102	14	1.3947285575346555
1102	15	1.458559124865289
1102	16	1.3608803350089644
1102	17	1.2914243387950464
1102	18	1.3743798570456676
1102	19	1.1622488332526686
1102	20	0.4069830168430002
1102	21	0.6849931360983987
1102	22	0.7402260558073408
1102	23	0.8113143170871382
1102	24	0.8476400297030864
1102	25	0.954924474992545
1103	1	0.7629521808135458
1103	2	0.7700881351260556
1103	3	1.0472280625470383
1103	4	0.821571501834697
1103	5	0.706784436536914
1103	6	0.5637215346461062
1103	7	0.4857423935993026
1103	8	0.7112867488729933
1103	9	0.7247717716213131
1103	10	0.7219847575254974
1103	11	0.6573064061263523
1103	12	0.919054925762758
1103	13	0.7162216722634867
1103	14	0.7118644164423316
1103	15	0.9813525995557981
1103	16	1.0028317179428896
1103	17	0.9731436609514148
1103	18	0.9948352518293078
1103	19	0.8920257043376694
1103	20	-0.052473773985084904
1103	21	0.3333357394983594
1103	22	-0.15737026877640403
1103	23	0.3505515643321857
1103	24	0.5437231361844752
1103	25	0.5992488865216714
1104	1	0.2971781874714914
1104	2	0.35600333535461814
1104	3	0.6363548474757827
1104	4	0.4590887339203036
1104	5	0.27472403280297897
1104	6	0.14454930558164847
1104	7	0.2002263563131308
1104	8	0.3473015813413838
1104	9	0.6625889478576568
1104	10	0.5441047904817395
1104	11	0.2990537084121243
1104	12	0.4186598175061107
1104	13	0.414025583784813
1104	14	0.7193557903483097
1104	15	0.6725115878966221
1104	16	0.5030037395642211
1104	17	0.4899016622498422
1104	18	0.5255593656531445
1104	19	-0.2723998525746083
1104	20	0.23973146884109298
1104	21	-0.4474361883127713
1104	22	-0.09668792426626283
1104	23	-0.4614823150575873
1104	24	-0.7799564118639779
1104	25	-1.982316330384684
1105	1	-0.15756836095943783
1105	2	0.060743248515088055
1105	3	0.47816954172824566
1105	4	0.2696691891416023
1105	5	0.14073480658013438
1105	6	0.13780780034372953
1105	7	0.17532273768935625
1105	8	0.35809857093106245
1105	9	0.6442660821661548
1105	10	-1.4674955555632394
1105	11	-0.43763576732330733
1105	12	0.21757287830908467
1105	13	0.2487093832511036
1105	14	0.5752334362075331
1105	15	-0.3117502902633973
1105	16	0.09415060507214434
1105	17	-0.11699822473656951
1105	18	-0.5516669666012959
1105	19	-1.7742082389611866
1105	20	-0.45371052042901994
1105	21	-1.72642830913383
1105	22	-0.8675275387981713
1105	23	-0.3712779749690114
1105	24	-0.6756226705987345
1105	25	-0.7119012498793467
1106	1	-0.5032719059036843
1106	2	-0.3779090033490675
1106	3	0.13786279433478654
1106	4	0.011443994898321819
1106	5	-0.3053602725109812
1106	6	-0.45632987866387253
1106	7	-0.34466059987880726
1106	8	-0.16198678891792184
1106	9	0.03791302595390533
1106	10	-0.4026977740870379
1106	11	-0.619214449230455
1106	12	-0.26442369048239556
1106	13	-0.2969338961055996
1106	14	-0.7071457040633788
1106	15	-0.8400145369052225
1106	16	-0.5725049236591317
1106	17	-0.4111155717298658
1106	18	-0.548833775666516
1106	19	-0.696862944431011
1106	20	-0.700775352489579
1106	21	-0.7462267489254018
1106	22	-0.8135411308624185
1106	23	-0.9956019037999582
1106	24	-0.7241866144315878
1106	25	-0.8228193000898827
1107	1	0.44001096901007486
1107	2	0.46967021891629557
1107	3	0.6704288302840204
1107	4	0.46267610997522723
1107	5	0.2807618802517595
1107	6	0.27047356624471064
1107	7	0.1412469891941157
1107	8	0.3156352349165985
1107	9	0.49065615973252363
1107	10	-1.3451741429289115
1107	11	-0.28012092562973123
1107	12	0.13577935816850584
1107	13	0.3286464632353585
1107	14	0.513809344827834
1107	15	0.6061527645866036
1107	16	0.44818174913601183
1107	17	0.01096328149052539
1107	18	-0.23618745021162368
1107	19	0.16133887166525795
1107	20	0.18339408856822104
1107	21	0.2578593454844338
1107	22	0.2513297238960419
1107	23	0.38976912624649174
1107	24	0.297446106917441
1107	25	0.013691086092826765
1108	1	-0.05942916980581714
1108	2	0.15492568779042415
1108	3	0.46089155791354486
1108	4	-0.055283376783158644
1108	5	0.025653872068204464
1108	6	0.025487477277437165
1108	7	-0.07257656859756878
1108	8	0.21374528015169147
1108	9	0.38096529821509506
1108	10	0.39355459512002966
1108	11	0.013697975870684331
1108	12	-0.04429390405189082
1108	13	0.07367739168722665
1108	14	0.47558075843044634
1108	15	-0.06362926256753809
1108	16	0.1307520512590301
1108	17	0.22165080721336494
1108	18	0.26892132525156853
1108	19	0.1599622202307529
1108	20	0.10420724680351157
1108	21	0.11708652558472465
1108	22	-0.02765068086133482
1108	23	0.12349114545532558
1108	24	0.18789859178541946
1108	25	-0.04277173219373509
1109	1	-0.0859982170566127
1109	2	-0.09274174242432665
1109	3	0.3037737494525423
1109	4	0.2222346098921193
1109	5	0.2363795637782502
1109	6	0.11293407385705478
1109	7	-0.011350914661974798
1109	8	-0.23315868910371762
1109	9	0.1044685200341533
1109	10	0.21382804087259188
1109	11	0.021064503364208065
1109	12	0.024581263166417955
1109	13	0.12494265626105516
1109	14	0.4079707125295968
1109	15	-0.43946529544572854
1109	16	-0.10813144275685005
1109	17	0.1310637963842396
1109	18	0.23114091702988304
1109	19	0.2950371790855968
1109	20	0.27697946654122774
1109	21	0.2945496542847934
1109	22	0.16140023796576486
1109	23	0.11110685827667055
1109	24	0.359208071810194
1109	25	0.1395988487849067
1110	1	-0.038871076067316324
1110	2	0.019054190031873475
1110	3	0.150405459131985
1110	4	-0.15553986728490088
1110	5	-0.07733030602264535
1110	6	-0.11649949898189504
1110	7	-0.2575847822945647
1110	8	-0.06913199361733291
1110	9	0.2660900656166376
1110	10	0.1931095325488883
1110	11	-0.11889585459625351
1110	12	0.0482775803641502
1110	13	-0.022798702124056547
1110	14	0.04252671585796719
1110	15	0.02983913528736082
1110	16	-0.28576156544679066
1110	17	0.0016047333349931137
1110	18	-5.0819599172946965
1110	19	-2.022058460240295
1110	20	-0.9148186922211323
1110	21	-0.5459933533012773
1110	22	-0.42860364385348504
1110	23	-0.4264225006026052
1110	24	-0.15557296259191844
1110	25	-0.22125806512542567
1111	1	-0.7016711340233677
1111	2	-0.513189626602248
1111	3	-1.74875888667464
1111	4	-1.163702577646177
1111	5	-0.7235203111359993
1111	6	-0.8045584816109468
1111	7	-0.9647023260896361
1111	8	-2.413060925401197
1111	9	-0.9513243091250345
1111	10	-0.5398898501441494
1111	11	-0.8340853472799665
1111	12	-0.3638106774946408
1111	13	-1.212843828286701
1111	14	-0.595507758926658
1111	15	-0.48910570783937857
1111	16	-2.6248122561248906
1111	17	-2.425500801928912
1111	18	-1.1360993652883238
1111	19	-0.6159051684405696
1111	20	-0.5433531340655264
1111	21	-0.53273581883888
1111	22	-0.5595170209711497
1111	23	-0.5988284456929946
1111	24	-0.5481686922563327
1111	25	-0.5016369000540735
1112	1	-0.440057941441772
1112	2	-0.45858014729931895
1112	3	0.025140445881124407
1112	4	-0.5864435017500824
1112	5	-2.05032496327323
1112	6	-0.9731401270104882
1112	7	-0.8201836851052917
1112	8	-0.3718399428835184
1112	9	-0.6126795940983598
1112	10	-0.3304687551225456
1112	11	-0.9224171981412397
1112	12	-1.508695719496437
1112	13	-1.7196798725478857
1112	14	-2.9035102254741645
1112	15	-4.218982318210735
1112	16	-1.4658139692014345
1112	17	-0.8107559957637491
1112	18	-0.3766697037540254
1112	19	-0.2703273000934203
1112	20	-0.3949976630923402
1112	21	-0.33287908783122333
1112	22	-0.3171661751135417
1112	23	-0.1911402989708577
1112	24	-0.33811630103024015
1112	25	-0.21582043784034255
1113	1	-0.2775731528613008
1113	2	0.010959465114567024
1113	3	-0.41777971571407235
1113	4	-2.609039020077603
1113	5	-0.5361912504153352
1113	6	-0.45292803331326326
1113	7	-0.455683191427525
1113	8	-0.8809093517587954
1113	9	-2.302752628113055
1113	10	-0.6771295925229239
1113	11	-0.8041688923050927
1113	12	-2.2870225614862676
1113	13	-1.5193141157786982
1113	14	-0.9342170785920132
1113	15	-0.16941485161825653
1113	16	-0.24646380616403007
1113	17	-0.08853865081996304
1113	18	0.14444974151771817
1113	19	0.235767454889654
1113	20	0.2288083044776812
1113	21	0.19750753610408367
1113	22	0.15189261350277405
1113	23	0.25840461717219654
1113	24	0.23236094712369493
1113	25	0.28452043236379154
1201	1	1.0444263490315038
1201	2	1.0631347988463844
1201	3	1.3263304655969428
1201	4	1.1496494636841064
1201	5	0.8910199470343585
1201	6	0.8170542340706746
1201	7	0.778291903212839
1201	8	0.940060662188202
1201	9	1.3601052120941546
1201	10	1.237728247684288
1201	11	0.9841329606536675
1201	12	1.2174554221758171
1201	13	1.3020271872697151
1201	14	1.512435541451815
1201	15	1.498502661830635
1201	16	1.3963411350593233
1201	17	1.4064445131569272
1201	18	1.5093620746518113
1201	19	1.2300530695539322
1201	20	1.1648819247207598
1201	21	1.1562121585388354
1201	22	1.0035619699482794
1201	23	1.0464358983038906
1201	24	1.0873044617680492
1201	25	1.1599385435413154
1202	1	0.8470522958094229
1202	2	0.8241643531688219
1202	3	1.120391896463591
1202	4	0.9532386464510552
1202	5	0.7593612596297064
1202	6	0.5806852978372632
1202	7	0.7021557292082221
1202	8	0.8650664272387658
1202	9	1.1131507249406312
1202	10	0.9259252584988147
1202	11	0.8219917101642409
1202	12	1.019731739564122
1202	13	1.0783997148460998
1202	14	1.196972298712133
1202	15	1.3769376733742433
1202	16	1.154033710700297
1202	17	1.1793751840359974
1202	18	1.2407888909127607
1202	19	1.0790792755566088
1202	20	0.98902711758668
1202	21	0.8438420341919581
1202	22	0.7683183661841184
1202	23	0.7348136913831311
1202	24	0.8550177213843426
1202	25	0.8055155481452587
1203	1	0.768905964950374
1203	2	0.7026558841992454
1203	3	1.007090590207536
1203	4	0.8563603644136961
1203	5	0.6765509311073927
1203	6	0.5967913380881527
1203	7	0.6697453697878046
1203	8	0.7672633432039717
1203	9	1.111493136734417
1203	10	0.8125669417005525
1203	11	0.6874201988567918
1203	12	0.9160245581281288
1203	13	1.0230579488238405
1203	14	1.186373209183877
1203	15	1.2262101506035492
1203	16	1.31204892052412
1203	17	1.2047556350568343
1203	18	1.1597008428410476
1203	19	1.028840301014064
1203	20	0.1788208601363337
1203	21	0.6784269095199349
1203	22	0.6644544191397941
1203	23	0.7429003289926257
1203	24	0.8759783029839454
1203	25	0.8456616760681896
1204	1	0.2738282530738836
1204	2	0.27931345684277886
1204	3	0.5521480750658903
1204	4	0.40469886355548823
1204	5	0.21582804528546262
1204	6	0.28255272531720976
1204	7	0.1996642797581316
1204	8	0.3685431125297569
1204	9	-0.6834028578256621
1204	10	-0.4780980770282426
1204	11	0.13162019133779523
1204	12	0.2954222251024845
1204	13	0.4008264263722907
1204	14	-1.5802352534881265
1204	15	0.038148574705182625
1204	16	0.3443793598118745
1204	17	0.37699253019167855
1204	18	0.6494856080579936
1204	19	0.5154641115606893
1204	20	-0.5402337433804618
1204	21	-0.5417935257067228
1204	22	-1.5465197230934677
1204	23	-1.176341800026357
1204	24	-0.219145061321953
1204	25	-2.700527330748713
1205	1	-0.30157095856426963
1205	2	-0.31521973146598015
1205	3	0.11104281186750953
1205	4	0.12091010210334474
1205	5	0.25259422772890616
1205	6	0.1188641229519547
1205	7	0.13790999251852298
1205	8	0.3179181929340942
1205	9	0.5655352438097623
1205	10	0.45265803982155006
1205	11	0.242142954493076
1205	12	0.3712190838455598
1205	13	0.5090206844290037
1205	14	0.5556715375272958
1205	15	0.09749246263835687
1205	16	-0.6803126171938558
1205	17	-1.0006347758658194
1205	18	-0.6663106841498418
1205	19	-0.6118447559873985
1205	20	-0.050430051914645446
1205	21	-0.35081223281809315
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2111	21	-0.41428638438875964
2111	22	-0.47448676992426897
2111	23	-0.36492228531925264
2111	24	-0.45229722907641445
2111	25	-0.3353728216234444
2112	1	-0.9645801644172494
2112	2	-0.3037590623125368
2112	3	-0.12930167641648893
2112	4	0.01681290737483465
2112	5	-0.5913887465635668
2112	6	-0.7409233569657374
2112	7	-0.3562008239985275
2112	8	0.0016780898821764367
2112	9	-2.3650189484134216
2112	10	-0.7347133168933553
2112	11	-1.0645858810999016
2112	12	-0.6842488740004882
2112	13	-0.21192401248729453
2112	14	-0.6309240481671665
2112	15	-0.7861155420774324
2112	16	-0.9798294970489394
2112	17	-0.8965757021581169
2112	18	-0.09318694495603097
2112	19	-0.04548835665510609
2112	20	-0.0033585666565159045
2112	21	-0.08786242551165202
2112	22	-0.04911542872203256
2112	23	-0.05785170589441435
2112	24	-0.027511980471494724
2112	25	0.2310443448980024
2113	1	0.1700797609117224
2113	2	-0.26054060159430037
2113	3	-0.02256492319870773
2113	4	0.14781274529979527
2113	5	-0.025484853687181896
2113	6	-0.6165562281914276
2113	7	-0.19650909232323244
2113	8	0.08915184735563031
2113	9	-0.8362996011857504
2113	10	-0.0100652014143634
2113	11	-0.15903435105644093
2113	12	-0.42011464780854624
2113	13	0.09886741058033266
2113	14	0.31760995434812145
2113	15	-2.0396866726900313
2113	16	-0.500671480392036
2113	17	-1.4867181633737658
2113	18	-0.12051348333930889
2113	19	0.14535318029590627
2113	20	0.1867098065807653
2113	21	0.18124881755630895
2113	22	0.2129233883588455
2113	23	0.3058232097856646
2113	24	0.2873845384882614
2113	25	0.5497125614198666
2114	1	-0.45940989378087593
2114	2	-0.4197215176498439
2114	3	-2.9334535787510916
2114	4	-1.1731388157636147
2114	5	-0.9067129318004774
2114	6	-1.4011533202453421
2114	7	-2.9447243829834875
2114	8	-1.108892338534858
2114	9	-0.5320568235082277
2114	10	-0.37552272501137196
2114	11	-0.4558857701937029
2114	12	-0.28272362268869955
2114	13	-2.81997687567144
2114	14	-2.2802464817551495
2114	15	-1.0946304855714928
2114	16	-1.4757187761068806
2114	17	-1.2876874979989452
2114	18	-0.29497597582506785
2114	19	-0.24515279760855435
2114	20	0.006616525140827889
2114	21	-0.047834324262531425
2114	22	-0.1298006991974745
2114	23	-0.131226120082637
2114	24	-0.0764106503071531
2114	25	-0.02218079674722162
2201	1	1.3213088248819425
2201	2	1.2995422922581064
2201	3	1.5238460783627694
2201	4	1.3513378526542752
2201	5	1.1483101131618767
2201	6	1.066622060900741
2201	7	1.0639113266657674
2201	8	1.2547768762882399
2201	9	1.612284368526062
2201	10	1.4513575591476666
2201	11	1.2658828968961302
2201	12	1.4923635889565716
2201	13	1.5428474715612026
2201	14	1.8071298229603414
2201	15	1.9129338506737383
2201	16	1.721206322551211
2201	17	1.8029228781005742
2201	18	1.858880022956754
2201	19	1.64837951707365
2201	20	1.5679371767755441
2201	21	1.4882951193939462
2201	22	1.5145497777181944
2201	23	1.421063093464582
2201	24	1.480532123141721
2201	25	1.6613097466124174
2202	1	1.171645569842859
2202	2	1.1518811163008138
2202	3	1.3630811305316861
2202	4	1.1789868263894405
2202	5	0.9478212972274278
2202	6	0.9099395118410882
2202	7	0.9223062004143685
2202	8	1.1343763670190299
2202	9	1.4702895735358936
2202	10	1.3701286046725087
2202	11	1.0439743306401468
2202	12	1.3627505118205079
2202	13	1.2694342333689121
2202	14	-0.688971310247851
2202	15	0.8076502375801837
2202	16	1.2440353976223903
2202	17	1.3897145959534498
2202	18	1.523306971222425
2202	19	1.4210741795752284
2202	20	1.338122246717063
2202	21	1.2377404392517448
2202	22	1.1422323125332596
2202	23	1.1351381577565718
2202	24	1.2571330013857889
2202	25	1.4105890220292423
2203	1	0.8523386777011872
2203	2	0.8130846412424972
2203	3	1.1724715666457115
2203	4	0.849263656481007
2203	5	0.6804760380133672
2203	6	0.6552773602738213
2203	7	0.616698552363026
2203	8	0.8001670325862378
2203	9	1.02546323744415
2203	10	0.8432018551461269
2203	11	0.7233463727667129
2203	12	0.9096259100143058
2203	13	0.9996131669701569
2203	14	0.8065022196775828
2203	15	1.0310209807817827
2203	16	0.9907682616003513
2203	17	1.0899590487256106
2203	18	1.2145733172106432
2203	19	1.0613436964572927
2203	20	0.24077037940242008
2203	21	0.6646997658495195
2203	22	-0.22447722429351558
2203	23	0.07949071956019083
2203	24	0.47645524134045303
2203	25	0.7358739904116902
2204	1	0.6967044201609021
2204	2	0.5572743061030181
2204	3	0.8728099055064504
2204	4	0.7757294853469929
2204	5	0.6086415889590313
2204	6	0.5320506221333758
2204	7	0.5060171601320818
2204	8	0.6189335717849502
2204	9	1.0246281175417593
2204	10	-1.397831943263597
2204	11	-0.04757139332492244
2204	12	0.4956130284266038
2204	13	0.6451567584231341
2204	14	0.8652522672983523
2204	15	0.8841723726572752
2204	16	0.8211845551752361
2204	17	0.8017677051137042
2204	18	1.0514606233256174
2204	19	0.9000579873790855
2204	20	-0.17852457327236948
2204	21	0.460308008086578
2204	22	0.5508987361506783
2204	23	0.6357055270211944
2204	24	0.7101754147167796
2204	25	0.13022949884850732
2205	1	0.32083793744309475
2205	2	0.11351908123066679
2205	3	0.43671741198389213
2205	4	0.2431426371772787
2205	5	0.11259970061411195
2205	6	-0.024971073954631606
2205	7	0.10067551959645726
2205	8	0.20899772512526482
2205	9	0.3624089083978532
2205	10	0.3899200558760967
2205	11	0.19362879814950418
2205	12	0.28081872553912035
2205	13	0.18461659785912587
2205	14	0.5226306796469586
2205	15	-0.019601234273260104
2205	16	0.06072475144294032
2205	17	0.320658299370713
2205	18	-0.2677041924144561
2205	19	-0.9491890216881558
2205	20	-0.2787750849362496
2205	21	-1.1217438854172102
2205	22	-1.8429421925558813
2205	23	-1.978385280517287
2205	24	-1.1475533470897616
2205	25	-1.8203851327823628
2206	1	-0.07557234795972789
2206	2	-0.43204691164875797
2206	3	0.26359331800158614
2206	4	0.0915844317011576
2206	5	-0.18152672437052786
2206	6	-0.30297218147066474
2206	7	-0.1680392979243308
2206	8	-0.2273431520738214
2206	9	0.3546203743779728
2206	10	-0.2865476348080165
2206	11	-0.26474953164673565
2206	12	0.005281174367240737
2206	13	0.035364548084686476
2206	14	-0.18445690109734159
2206	15	-0.315013566551265
2206	16	-0.7640306962602708
2206	17	-0.4816411300555785
2206	18	-0.08035718618685195
2206	19	-0.786581140348936
2206	20	-0.6286104941373338
2206	21	-2.6869314093360757
2206	22	-1.5272409203620754
2206	23	-2.3400154165504077
2206	24	-1.2526217405536784
2206	25	-1.9860041240729913
2207	1	0.6352735987937734
2207	2	0.47044671704072627
2207	3	0.8203289731041821
2207	4	0.5002540938230737
2207	5	0.4724522966017446
2207	6	0.4653619690210178
2207	7	0.38778271585383095
2207	8	0.44094141201657067
2207	9	-1.2666698436611128
2207	10	-0.10296585526359792
2207	11	0.19942531440003108
2207	12	0.5484314697991266
2207	13	0.6980088168159568
2207	14	0.8940423335731609
2207	15	0.9392134551118616
2207	16	0.9001390430108955
2207	17	0.8418412219971785
2207	18	0.8780691494939248
2207	19	-0.33749287150539686
2207	20	0.30619882357665773
2207	21	-0.2779457898379718
2207	22	0.2940098115905343
2207	23	-0.4602268537674732
2207	24	-1.633424650795753
2207	25	-0.2632360653189707
2208	1	0.46282636586546033
2208	2	0.18692631032503826
2208	3	0.6887278731680162
2208	4	0.5444890150592272
2208	5	0.45002261942456556
2208	6	0.3490509093647418
2208	7	0.4080399830899495
2208	8	0.5746657586678054
2208	9	-0.2072218085063966
2208	10	0.06928500967321582
2208	11	0.2914956271519884
2208	12	0.2504247212520525
2208	13	0.5750801510977581
2208	14	0.9155265921009743
2208	15	0.8274754539523457
2208	16	0.5339987745181105
2208	17	0.46698496725284144
2208	18	0.7014398054348021
2208	19	-1.4362631141886766
2208	20	-0.07535589696558276
2208	21	-0.12970291416280588
2208	22	0.28287573380998765
2208	23	0.36678987427342236
2208	24	0.5725903654357438
2208	25	0.6104843424176494
2209	1	0.2428572684727115
2209	2	-1.3398251835857486
2209	3	-0.17657742136060506
2209	4	0.1568570178657609
2209	5	0.1126350418839337
2209	6	0.23402604102687263
2209	7	0.2497683470716865
2209	8	0.3889186496542081
2209	9	-2.007676608544706
2209	10	-0.2745686492227577
2209	11	0.06295604888150308
2209	12	0.38944565849737245
2209	13	0.45266398856703915
2209	14	0.7272879544993316
2209	15	0.8513052767396729
2209	16	0.695140979651292
2209	17	0.47544875309340995
2209	18	0.7190527788866774
2209	19	0.4746531134735932
2209	20	0.43352690183186127
2209	21	0.5901063797250004
2209	22	0.4435992003068989
2209	23	0.37929398712296347
2209	24	0.5345112726939121
2209	25	0.5664326397253703
2210	1	0.10404954295589874
2210	2	0.08958839618936665
2210	3	0.038892586135965246
2210	4	0.21731293600328172
2210	5	0.07023485680990404
2210	6	0.05514113724598246
2210	7	0.034069991258363075
2210	8	0.11146862901776444
2210	9	0.2956277523408204
2210	10	-1.5622010112694085
2210	11	-0.5293173154141115
2210	12	-2.427168131377101
2210	13	-0.8268765380587055
2210	14	-0.6506667411315874
2210	15	-0.8971522217131849
2210	16	-0.219664280523169
2210	17	-0.053407658364726274
2210	18	0.24568392102696635
2210	19	0.16426880435962943
2210	20	0.1730413882396178
2210	21	0.1147928105241931
2210	22	0.10642325911076966
2210	23	0.001939568630312749
2210	24	0.09057145421584778
2210	25	0.04610926131753956
2211	1	-0.35680028866002544
2211	2	-0.12089209149420554
2211	3	-1.5462759098165932
2211	4	-0.6834549837344142
2211	5	-0.5813149454793702
2211	6	-0.4901008314988218
2211	7	-0.5049724551601358
2211	8	-0.21165507228293023
2211	9	-0.010923776293708443
2211	10	0.014036055511986945
2211	11	-0.32443258455152346
2211	12	-0.3544298610163992
2211	13	-0.34489949591630875
2211	14	-0.9228931364250457
2211	15	-0.5000629893811492
2211	16	-0.2097310642684178
2211	17	-0.24124888042349113
2211	18	-0.07205421767905307
2211	19	-0.15271965671494314
2211	20	-0.20952416889190317
2211	21	-0.2730648941243601
2211	22	-0.2684605211184561
2211	23	-0.6222624113325494
2211	24	-0.41163125848053994
2211	25	-0.32630906137208626
2212	1	-0.28857454462354326
2212	2	-0.2550990834464777
2212	3	-2.2694691003420324
2212	4	-1.2376146752154256
2212	5	-0.545911752186889
2212	6	-0.30706321773578793
2212	7	-0.23507104840211923
2212	8	-0.2567990339690809
2212	9	-0.812168757010916
2212	10	-0.14096931391407708
2212	11	-0.20904130208091942
2212	12	0.08800948830629096
2212	13	-0.3221313726690056
2212	14	-0.012016648003054797
2212	15	0.20961641135189524
2212	16	-1.0355260933333739
2212	17	-1.2736851944902057
2212	18	-0.16631646486050755
2212	19	-0.03648261281553289
2212	20	-0.16762950129736964
2212	21	0.005786778735433984
2212	22	-0.3127998326816197
2212	23	-0.013021270086248649
2212	24	-0.14305019162145527
2212	25	0.0893012398262627
2213	1	-3.131342385553964
2213	2	-1.4126690334435033
2213	3	-2.4038063205916345
2213	4	-0.9701816879000376
2213	5	-0.8530764293648119
2213	6	-0.5597421992436864
2213	7	-0.38926434797289033
2213	8	-0.13982911204818294
2213	9	-0.2315593676819816
2213	10	-0.13810764683288568
2213	11	-0.45188639051309565
2213	12	-0.17911069431062288
2213	13	-0.6552215321383628
2213	14	-0.12670465964854571
2213	15	-0.27270337118017807
2213	16	-1.6360610656616004
2213	17	-1.02585903455698
2213	18	-0.23536947533870034
2213	19	-0.0972971486209886
2213	20	-0.11075249000130327
2213	21	0.07151589597005881
2213	22	0.09842176884792053
2213	23	0.11369808764177947
2213	24	0.10813713109075707
2213	25	0.144310768925493
2214	1	-0.7885318471285814
2214	2	-0.7048517698996868
2214	3	-6.571735058662789
2214	4	-2.094707064824483
2214	5	-1.2832689937537012
2214	6	-0.7397879017988416
2214	7	-0.5765940718192866
2214	8	-0.5074880214176005
2214	9	-0.11855089340496505
2214	10	-0.2187009521618961
2214	11	-0.8357430065935816
2214	12	-0.4933955678163997
2214	13	-5.13617512568225
2214	14	-3.804144516137498
2214	15	-2.2509561861954737
2214	16	-2.850735635061767
2214	17	-3.472823746126302
2214	18	-1.1638599255920496
2214	19	-0.6888489441962236
2214	20	-0.32372437633816986
2214	21	-0.4158543786925648
2214	22	-0.32947111108007476
2214	23	-0.3187330897561722
2214	24	-0.2265111773889359
2214	25	-0.04424322859633634
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2209.0
3	255.0
4	291.0
5	624.0
6	89.0
7	111.0
8	297.0
9	899.0
10	2060.0
11	4628.0
12	8839.0
13	14590.0
14	21874.0
15	28696.0
16	34790.0
17	38289.0
18	40236.0
19	41441.0
20	41801.0
21	42552.0
22	44573.0
23	46967.0
24	50112.0
25	54660.0
26	60452.0
27	69338.0
28	80081.0
29	97400.0
30	119516.0
31	147661.0
32	191636.0
33	250334.0
34	330143.0
35	446376.0
36	616512.0
37	912316.0
38	1729201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.80876061696429	20.830827086580676	12.968362721995971	23.392049574459065
2	15.033964901468655	21.43490642660218	40.7706312826179	22.76049738931127
3	18.180247697955533	27.59271254348236	29.56897711235263	24.658062646209476
4	12.30562700352599	17.32991173172881	38.38626508637562	31.978196178369565
5	13.212917122831753	38.164753810912515	34.89620265347572	13.72612641278001
6	30.588571248972922	38.80509520965881	17.577377771990715	13.028955769377557
7	25.638515502975924	33.37489083075305	23.701027389695284	17.285566276575743
8	24.098620202171126	37.188094430811844	21.600322680750068	17.11296268626696
9	24.80091004341528	16.92689372973047	21.932204351728764	36.33999187512549
10	15.225829045553729	29.837360703285654	33.18595942997225	21.750850821188365
11	32.421095845096815	26.296141122646173	22.978403439531373	18.30435959272564
12	20.890183585571112	27.631579656261263	31.860816395213163	19.61742036295446
13	26.56819709526318	24.06593116492435	26.988788246722144	22.37708349309033
14	20.356666621769058	23.208724503769155	28.298676867403888	28.135932007057907
15	23.292602459656752	30.091912959802762	24.20275232422441	22.412732256316076
16	21.8440296340355	31.044818308982215	25.737999941454586	21.3731521155277
17	20.341311969966387	29.961310385960466	28.650662220435073	21.046715423638073
18	20.78072162697218	29.49191717043003	29.186882352571146	20.540478850026645
19	21.249644165983437	28.60557067694223	29.373794991114256	20.770990165960086
20	22.757394147969343	27.966328922648724	28.209584353781935	21.066692575599998
21	22.84970596464536	28.815482713278445	27.661322561076133	20.673488761000062
22	21.659247541595413	28.41515922473286	28.68299125617228	21.24260197749945
23	21.43020830040775	28.834998290264274	28.542726870436514	21.19206653889146
24	21.545088768279612	27.99397500445894	28.474965213122722	21.98597101413873
25	22.146939838449693	27.88325992178415	27.766124723303566	22.203675516462596
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5374.0
1	5374.0
2	4991.0
3	4608.0
4	4608.0
5	4608.0
6	7149.0
7	9690.0
8	9690.0
9	9690.0
10	14537.0
11	19384.0
12	19384.0
13	19384.0
14	32148.5
15	44913.0
16	44913.0
17	44913.0
18	74086.5
19	103260.0
20	103260.0
21	103260.0
22	150687.5
23	198115.0
24	198115.0
25	198115.0
26	261731.5
27	325348.0
28	325348.0
29	325348.0
30	394268.0
31	463188.0
32	463188.0
33	463188.0
34	521365.0
35	579542.0
36	579542.0
37	579542.0
38	619086.5
39	658631.0
40	658631.0
41	658631.0
42	658521.0
43	658411.0
44	658411.0
45	658411.0
46	671981.5
47	685552.0
48	685552.0
49	685552.0
50	636722.5
51	587893.0
52	587893.0
53	587893.0
54	522313.0
55	456733.0
56	456733.0
57	456733.0
58	392963.5
59	329194.0
60	329194.0
61	329194.0
62	277042.5
63	224891.0
64	224891.0
65	224891.0
66	172709.0
67	120527.0
68	120527.0
69	120527.0
70	89936.5
71	59346.0
72	59346.0
73	59346.0
74	41911.5
75	24477.0
76	24477.0
77	24477.0
78	16633.0
79	8789.0
80	8789.0
81	8789.0
82	5787.5
83	2786.0
84	2786.0
85	2786.0
86	1783.0
87	780.0
88	780.0
89	780.0
90	531.0
91	282.0
92	282.0
93	282.0
94	184.0
95	86.0
96	86.0
97	86.0
98	67.5
99	49.0
100	49.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1033229723203195
2	0.05405745920250172
3	0.057646931925111396
4	0.05235245965926213
5	0.049821881389822305
6	0.06952808663694943
7	0.06568735082375707
8	0.06276193055483019
9	0.06753592927590106
10	0.06638729800466595
11	0.061308194102173266
12	0.06267219373676494
13	0.06274398319121713
14	0.06504124573368733
15	0.06739235036699666
16	0.06321061464515638
17	0.06618987700492242
18	0.1416764883614039
19	0.07078440208986281
20	0.06511303518813952
21	0.062295299100890926
22	0.06832561327487519
23	0.06706929782196179
24	0.07853766317069971
25	0.07042545481760185
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5571849.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.78833528489096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.3989841794535	49.22541704654389
2	13.405962463091402	16.834761318984917
3	4.050687579593328	7.63007789645548
4	1.7113104710470661	4.298013425305915
5	0.8216574184723482	2.579525074017988
6	0.4487201265549657	1.6904633853127133
7	0.25662494508439987	1.1279137166098223
8	0.17743119094605894	0.8912487285694928
9	0.11515642433098869	0.6507432162992964
>10	0.5554071907940669	6.058924848437347
>50	0.031742570313337	1.3789351098681422
>100	0.02203692379630406	2.731596623643303
>500	0.001907993146569514	0.8575287943829342
>1k	0.0020814351592496395	2.5062501183352337
>5k	2.0236175157604404E-4	0.793178473045578
>10k+	8.672646496116173E-5	0.7454222241878976
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	15327	0.2750792420971925	No Hit
CCCATGTACTCTGCGTTGATACCAC	14989	0.26901303319598213	No Hit
GTATCAACGCAGAGTACTTTTTTTT	10751	0.19295210620388314	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6928	0.12433933511119917	No Hit
GAGTACATGGGAAGCAGTGGTATCA	6826	0.12250870402266824	No Hit
GTACTGGTTCACTATCGGTCAGTCA	6606	0.11856028402779759	No Hit
ATTCCATTCCATTCCATTCCATTCC	6547	0.11750138957462775	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6139	0.110178865220504	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.794736361304838E-5	0.0
4	0.0	0.0	0.0	3.589472722609676E-5	0.0
5	0.0	0.0	0.0	7.178945445219351E-5	0.0
6	0.0	0.0	0.0	7.178945445219351E-5	0.0
7	0.0	0.0	0.0	1.2563154529133866E-4	0.0
8	0.0	0.0	0.0	1.9742099974353217E-4	0.0
9	0.0	0.0	0.0	2.1536836335658054E-4	0.0
10	0.0	0.0	0.0	2.3331572696962894E-4	0.0
11	0.0	0.0	0.0	2.3331572696962894E-4	0.0
12	0.0	0.0	0.0	2.3331572696962894E-4	0.0
13	1.794736361304838E-5	0.0	0.0	2.512630905826773E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACCG	100	2.4082148E-5	10.447328	5
ACACCGT	185	6.002665E-11	10.267274	6
AATCGCC	205	3.6379788E-12	10.200118	18
AAATCGC	225	0.0	10.137571	17
ACGGTCC	115	1.0311243E-5	9.910241	8
CGCCCTA	945	0.0	9.654656	16
GTACTGG	2175	0.0	9.522826	1
TATTCCG	265	0.0	9.318371	5
TACTGGT	2020	0.0	9.1685295	2
TATACTG	1215	0.0	9.145808	5
TGTCCCG	980	0.0	9.109179	11
GCCGCTA	115	1.10682624E-4	9.090592	16
GTCCCGC	1050	0.0	9.050995	12
ACCGTCC	105	4.5088076E-4	9.045061	8
GTCCTAG	505	0.0	9.030638	1
ATTAGAC	360	0.0	8.970009	3
CCGCCCT	1040	0.0	8.955505	15
CGGGACT	85	0.0074543124	8.939051	4
TAGACAG	750	0.0	8.864399	5
GTATTAT	1070	0.0	8.79063	1
>>END_MODULE