FastQCFastQC Report
Wed 25 May 2016
SRR1294589_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294589_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5571849
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT279520.5016647077119283No Hit
GGTATCAACGCAGAGTACTTTTTTT196030.3518221689065874No Hit
TATCAACGCAGAGTACTTTTTTTTT175570.31510186295429043No Hit
CCCATGTACTCTGCGTTGATACCAC172740.3100227590517977No Hit
GTACATGGGAAGCAGTGGTATCAAC150350.2698386119221824No Hit
ACGCAGAGTACTTTTTTTTTTTTTT108190.19417252692957043No Hit
GAGTACATGGGAAGCAGTGGTATCA71440.12821596565161764No Hit
ATTCCATTCCATTCCATTCCATTCC66220.11884744184560637No Hit
GTACTGGTTCACTATCGGTCAGTCA63160.11335554858001357No Hit
CATGTACTCTGCGTTGATACCACTG62900.11288891712607431No Hit
GAATGGAATGGAATGGAATGGAATG56440.10129492023204505No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG855.2051844E-511.2021619
TAGCCCG600.00577655511.109515
AATCGCC1900.010.97858318
GTCCTAG4350.010.9290771
GACCGTC951.6068429E-410.0236197
CGCCCTA9700.09.77474716
GCTAGAC2550.09.685063
TAGACCG900.00109067449.5224385
TATACCG1401.0265612E-69.5224385
TACACCG1901.0095391E-99.5224385
ATCGCCG1901.0913936E-99.48167419
ATACCGA1451.6547601E-69.1940786
GTACTGG20950.09.1679061
TGTCCCG10450.09.07915811
ACCGACC1054.3913003E-49.0689888
GACTCGC950.0017862389.0212566
TACCGGT950.0017862389.0212567
GTCCCGC10800.08.86672312
AAATCGC2151.0822987E-98.82000417
CGGGGGA4950.08.81109417
Produced by FastQC (version 0.11.5)