##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294589_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5571849 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.193879087534498 31.0 31.0 34.0 28.0 34.0 2 31.419351637131587 31.0 31.0 34.0 28.0 34.0 3 31.493692847742285 31.0 31.0 34.0 28.0 34.0 4 35.12728072853374 37.0 35.0 37.0 32.0 37.0 5 35.30020662799728 37.0 35.0 37.0 32.0 37.0 6 34.81823376764159 37.0 35.0 37.0 32.0 37.0 7 34.67086455501576 37.0 35.0 37.0 31.0 37.0 8 34.68302156070633 37.0 35.0 37.0 31.0 37.0 9 36.16283822479755 39.0 35.0 39.0 31.0 39.0 10 35.91608064037629 39.0 35.0 39.0 30.0 39.0 11 36.08827644108805 39.0 35.0 39.0 30.0 39.0 12 36.04397283558833 39.0 35.0 39.0 30.0 39.0 13 35.90462447923481 39.0 35.0 39.0 30.0 39.0 14 36.97222160902063 40.0 36.0 41.0 30.0 41.0 15 37.01160566268038 40.0 36.0 41.0 30.0 41.0 16 37.045152156851344 40.0 36.0 41.0 30.0 41.0 17 37.08534186766368 40.0 36.0 41.0 30.0 41.0 18 37.09072571780032 40.0 36.0 41.0 30.0 41.0 19 37.09897253138052 40.0 36.0 41.0 30.0 41.0 20 37.17335412355934 40.0 37.0 41.0 30.0 41.0 21 37.00647738300159 40.0 36.0 41.0 30.0 41.0 22 36.996585155125345 40.0 36.0 41.0 30.0 41.0 23 37.00657950350055 40.0 36.0 41.0 30.0 41.0 24 36.94192412608454 40.0 36.0 41.0 30.0 41.0 25 35.80862815916225 39.0 34.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5189655390125552 1101 2 0.5651070252687695 1101 3 0.4492080549877606 1101 4 0.4250319428032512 1101 5 0.4301478950274884 1101 6 0.518346795859145 1101 7 0.5747580884223211 1101 8 0.5495618456600582 1101 9 0.7332858781610909 1101 10 0.8555455850003852 1101 11 0.7519512489567646 1101 12 0.8957212931997702 1101 13 0.8740942784874122 1101 14 1.0857976839095969 1101 15 1.023085394996322 1101 16 1.0831728254691342 1101 17 1.111055834785553 1101 18 1.0332255565194188 1101 19 1.2200065354404188 1101 20 1.1228349374167195 1101 21 1.4058671587814544 1101 22 1.3699425034699075 1101 23 1.256378174021556 1101 24 1.350757257633532 1101 25 1.3247486616234525 1102 1 0.44400669563367146 1102 2 0.44529257457690363 1102 3 0.34778163903886394 1102 4 0.30846732599901117 1102 5 0.377485789852372 1102 6 0.38863695050822855 1102 7 0.45474595804977724 1102 8 0.49854725924444665 1102 9 0.5930724894348174 1102 10 0.7649689810206866 1102 11 0.6598738342583985 1102 12 0.6770944002114945 1102 13 0.729489805246395 1102 14 0.897206315583766 1102 15 0.893743510966651 1102 16 0.852417252726589 1102 17 0.8151872836854466 1102 18 0.8190545127493891 1102 19 0.9223806853331524 1102 20 0.9180113901993678 1102 21 1.1634452873962573 1102 22 1.1676156888638545 1102 23 1.0539224569659424 1102 24 1.1032338335631522 1102 25 1.0387603955081062 1103 1 0.307242298007548 1103 2 0.296823274104689 1103 3 0.19277940432503726 1103 4 0.21516098455185784 1103 5 0.22646986362277488 1103 6 0.3679580869974757 1103 7 0.3487165697638872 1103 8 0.3491996077625785 1103 9 0.27306811710898415 1103 10 0.5508119288577049 1103 11 0.5489391223259048 1103 12 0.5573447285231907 1103 13 0.6624951887677284 1103 14 0.8181031200107114 1103 15 0.761981653876397 1103 16 0.7176500633656389 1103 17 0.7007957381540351 1103 18 0.7040859239485329 1103 19 0.8900676961998855 1103 20 0.7323558639202119 1103 21 0.9476960398047893 1103 22 0.8828128059542308 1103 23 0.8818642479573882 1103 24 0.9437838664171139 1103 25 0.8752970517500671 1104 1 0.21543447927388826 1104 2 0.18817492666140367 1104 3 0.08652292687905572 1104 4 0.1263984505286473 1104 5 0.1799700627662446 1104 6 0.19462459418918598 1104 7 0.29774037479410964 1104 8 0.25489280833429717 1104 9 -0.01460620730465223 1104 10 0.38820184346000985 1104 11 0.41866005235331016 1104 12 0.45729199042521174 1104 13 0.00523187383607393 1104 14 0.42522622076990046 1104 15 0.5721813842415457 1104 16 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0.2761586949893271 2207 5 0.20375156611456902 2207 6 0.36199488512777833 2207 7 0.3290408503415918 2207 8 0.38001586569568246 2207 9 0.5076465582260781 2207 10 0.66579278206585 2207 11 0.5264966260627943 2207 12 0.5192267936004811 2207 13 0.5084958679743607 2207 14 0.5774705087016372 2207 15 0.5463263699554588 2207 16 0.6454405323922145 2207 17 0.6872873872309881 2207 18 0.6762216787395019 2207 19 0.8097541855797914 2207 20 0.6440849253332246 2207 21 0.9737406583229244 2207 22 0.9284575552296772 2207 23 0.7888667343660956 2207 24 0.8701513813437955 2207 25 0.9034625854528429 2208 1 0.21294538840064448 2208 2 0.16513029770728593 2208 3 0.17106698628485617 2208 4 0.1738948083664411 2208 5 0.08537459045943052 2208 6 0.2270896792589454 2208 7 0.21123221170906703 2208 8 0.14157099065531753 2208 9 0.3000935291932265 2208 10 0.389562311478727 2208 11 -0.01632517295276159 2208 12 -0.1973988173141379 2208 13 0.07604740279970912 2208 14 -0.4028131066046683 2208 15 0.22904515966636296 2208 16 0.3335454653307508 2208 17 0.22214662722465306 2208 18 0.3064375462181772 2208 19 0.5076793779382385 2208 20 0.4136639367194732 2208 21 0.7044742077649886 2208 22 0.7315126318531213 2208 23 0.6848807577568579 2208 24 0.7323179244626701 2208 25 0.7375436068766348 2209 1 0.038684850923512926 2209 2 0.04813607395855257 2209 3 0.10935482916404737 2209 4 -0.5354590380864437 2209 5 -0.004443828115242354 2209 6 0.15509448911793555 2209 7 0.1917763916683981 2209 8 0.23294051194393006 2209 9 0.30359956589230563 2209 10 0.32586205893134945 2209 11 0.3431131642443148 2209 12 0.35827417967915665 2209 13 0.41374977498315957 2209 14 0.46753413781357267 2209 15 -0.17333894497549807 2209 16 -0.1624731317247381 2209 17 0.21685546099060815 2209 18 -0.12331891038736131 2209 19 0.32420101783617383 2209 20 0.2865381205960986 2209 21 0.6223343362379481 2209 22 0.6913855947679579 2209 23 0.5355587371959771 2209 24 0.6520471402490458 2209 25 0.6891946093146402 2210 1 -0.14785997271641804 2210 2 -0.19821957988430938 2210 3 -0.11950011670447935 2210 4 -0.04943485333642883 2210 5 -0.1058419612062238 2210 6 -0.6200166002106968 2210 7 -0.19287998824016483 2210 8 -0.34433507201080005 2210 9 -0.11648247875964302 2210 10 0.053577067624814845 2210 11 0.02304559425964925 2210 12 -0.05587283377227692 2210 13 0.039869560301070806 2210 14 0.017832148913917933 2210 15 -0.03604497653862637 2210 16 0.08315340704585594 2210 17 -1.059514609211135 2210 18 -1.5835988553379252 2210 19 -0.3578985858400259 2210 20 -0.21751819807288086 2210 21 -0.4519908424812513 2210 22 -0.344702721346799 2210 23 -0.26440743605026285 2210 24 0.059381675575984616 2210 25 0.0554353751151524 2211 1 -0.24648255153214294 2211 2 -0.2265657938808232 2211 3 -0.17656880101595007 2211 4 -0.11262040802770201 2211 5 -0.08450449052308784 2211 6 -0.08084209528279729 2211 7 -0.16616478419250313 2211 8 -0.16106024431670107 2211 9 -0.09296930622413413 2211 10 -0.07748841519082816 2211 11 -0.38959759051294185 2211 12 -0.2806019516805236 2211 13 -0.22803537191200718 2211 14 -0.24484771168128106 2211 15 -0.32866251110498723 2211 16 -0.29575119382596426 2211 17 -0.4142011348711705 2211 18 -0.45568111862495897 2211 19 -0.26532979029168047 2211 20 -0.41201075496800144 2211 21 -0.07414798411338097 2211 22 -0.14334627013739265 2211 23 -0.8988057026422283 2211 24 -0.297668865524777 2211 25 -0.9888027915494604 2212 1 -0.19486266661253637 2212 2 -0.31942862251415605 2212 3 -0.1954286414284141 2212 4 -0.08970649906314065 2212 5 -0.115190221420292 2212 6 -0.17195252009038597 2212 7 -0.2506826483115816 2212 8 -0.4200292143307962 2212 9 -0.18685965994691145 2212 10 -0.48229493343144014 2212 11 -0.17591319013354934 2212 12 -0.31110034149215693 2212 13 -0.261963558338401 2212 14 -0.2770190181319734 2212 15 -0.13128842848925615 2212 16 -0.3472307994032633 2212 17 -0.5428040226060276 2212 18 -0.32845856363217507 2212 19 -0.39170250736194845 2212 20 -0.7061987300327175 2212 21 -0.1393281610499031 2212 22 -0.4761235069681007 2212 23 -0.38452406662646865 2212 24 -0.6024176591934847 2212 25 -0.31199938004111516 2213 1 -0.19675254104815565 2213 2 -0.20112329155915631 2213 3 -0.1356382739820745 2213 4 -0.012731289979903693 2213 5 -0.06348234591350632 2213 6 -0.27643123487506216 2213 7 -0.34982453347105746 2213 8 -0.35327917122478425 2213 9 -0.25274144113316055 2213 10 -0.24825350435646953 2213 11 -0.017395174871730035 2213 12 0.11869606079604722 2213 13 0.12578750557904783 2213 14 -0.6996557404880477 2213 15 -0.23549931378758515 2213 16 -0.3045477209167444 2213 17 -0.209632839090375 2213 18 -0.11907476600002553 2213 19 -0.22090126194148496 2213 20 -0.30959723864095423 2213 21 -0.012986000011942167 2213 22 -0.32335098922597183 2213 23 -0.48265616481357654 2213 24 -0.6160706687561515 2213 25 -0.37104435677912306 2214 1 -0.26995051142781534 2214 2 -0.2574450990701038 2214 3 -0.21181548359938773 2214 4 -0.1287280867204572 2214 5 -0.14931649601783903 2214 6 -0.21271785910887075 2214 7 -0.2288182493697235 2214 8 -0.19393594534947312 2214 9 -0.4057163447489458 2214 10 -0.2098068379984639 2214 11 -0.12186653504851819 2214 12 -0.3052646651400721 2214 13 -0.3855996619219155 2214 14 -0.44405680473607845 2214 15 -0.1634608966466189 2214 16 -0.3423602486274504 2214 17 -0.5004534447904803 2214 18 -0.4048348770233403 2214 19 -0.5209495030915221 2214 20 -0.57970102675813 2214 21 -0.588521027520585 2214 22 -0.9511349489160708 2214 23 -1.2270768276418806 2214 24 -0.708770933287937 2214 25 -1.0643452716934263 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 9.0 9 73.0 10 156.0 11 368.0 12 983.0 13 2408.0 14 5052.0 15 8719.0 16 12905.0 17 16574.0 18 19736.0 19 22096.0 20 24491.0 21 26345.0 22 28980.0 23 32438.0 24 36109.0 25 41068.0 26 46987.0 27 52881.0 28 62916.0 29 78097.0 30 99188.0 31 128744.0 32 171769.0 33 229403.0 34 312522.0 35 431801.0 36 613836.0 37 967723.0 38 2097469.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.706685857674195 20.946642196455365 12.157378717239714 25.18929322863072 2 14.851245968797791 21.87273919303987 39.2041133921612 24.07190144600114 3 17.889038887175648 27.012954245011418 29.953016511073393 25.144990356739537 4 12.031898483156333 17.451007633220573 38.28622118067036 32.23087270295274 5 13.394360071036301 37.68058186147347 34.93217067309462 13.992887394395614 6 30.984062889731167 38.68536610407765 17.109561619974915 13.22100938621627 7 25.74787674950958 33.4800233507291 23.474429802768196 17.297670096993127 8 24.250268722818067 37.13277240810828 21.26414250315395 17.3528163659197 9 24.75727928819154 16.07665767621301 22.07301148131424 37.09305155428121 10 15.146321937272141 29.561119777115607 33.23322731717619 22.059330968436065 11 32.73309357505029 25.703480326256116 22.841466090892407 18.721960007801183 12 21.00102929682067 27.30316156550034 31.954614022666068 19.74119511501293 13 26.890567327237687 23.472651613127855 27.144791869696327 22.491989189938128 14 20.422338167386396 22.622169424517182 28.68117271448265 28.274319693613776 15 23.449271398234814 29.490100808217427 24.42879890189254 22.631828891655218 16 21.80510731990798 30.563210110257895 25.994024242593927 21.6376583272402 17 20.176070847589525 29.66498727172744 28.927960705317158 21.230981175365876 18 20.555774713711447 29.061262860685165 29.682190362728484 20.7007720628749 19 21.075834798742672 28.113371173738145 30.13059282941917 20.68020119810001 20 22.481320799111387 27.361056197065274 29.163602896637897 20.994020107185438 21 22.535771428981654 28.415970681113134 28.359310711821152 20.68894717808406 22 21.30357364961747 28.052484529404765 29.404567619320428 21.23937420165734 23 21.146242328164067 28.402784645146912 29.33183125649135 21.11914177019768 24 21.23596673204891 27.608178182861735 29.311239410831124 21.844615674258222 25 21.875986564328706 27.444359790980727 28.600480819708764 22.079172824981804 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2222.0 1 2222.0 2 2545.0 3 2868.0 4 2868.0 5 2868.0 6 6546.5 7 10225.0 8 10225.0 9 10225.0 10 14384.5 11 18544.0 12 18544.0 13 18544.0 14 28370.5 15 38197.0 16 38197.0 17 38197.0 18 66396.0 19 94595.0 20 94595.0 21 94595.0 22 143338.5 23 192082.0 24 192082.0 25 192082.0 26 257568.0 27 323054.0 28 323054.0 29 323054.0 30 393723.0 31 464392.0 32 464392.0 33 464392.0 34 524143.5 35 583895.0 36 583895.0 37 583895.0 38 620288.0 39 656681.0 40 656681.0 41 656681.0 42 656485.5 43 656290.0 44 656290.0 45 656290.0 46 673122.5 47 689955.0 48 689955.0 49 689955.0 50 642392.0 51 594829.0 52 594829.0 53 594829.0 54 529380.5 55 463932.0 56 463932.0 57 463932.0 58 398694.0 59 333456.0 60 333456.0 61 333456.0 62 281326.5 63 229197.0 64 229197.0 65 229197.0 66 175719.0 67 122241.0 68 122241.0 69 122241.0 70 90980.0 71 59719.0 72 59719.0 73 59719.0 74 41807.0 75 23895.0 76 23895.0 77 23895.0 78 16069.0 79 8243.0 80 8243.0 81 8243.0 82 5336.5 83 2430.0 84 2430.0 85 2430.0 86 1545.0 87 660.0 88 660.0 89 660.0 90 426.5 91 193.0 92 193.0 93 193.0 94 118.0 95 43.0 96 43.0 97 43.0 98 27.0 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09607223742064797 2 0.0 3 0.13175159628338817 4 0.012114470438807656 5 0.021106099608944894 6 0.012581101892746914 7 0.020549731336940394 8 0.018898573884539943 9 2.3331572696962894E-4 10 0.3960265254855255 11 0.06215172019198654 12 0.0015075785434960638 13 5.3842090839145136E-5 14 0.001040947089556806 15 0.0014537364526569189 16 5.3842090839145136E-5 17 1.794736361304838E-5 18 4.1278936310011274E-4 19 4.845788175523063E-4 20 1.7947363613048377E-4 21 2.512630905826773E-4 22 0.0026921045419572567 23 1.4357890890438703E-4 24 0.0 25 0.0020459994518875154 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 5571849.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.885437881637436 #Duplication Level Percentage of deduplicated Percentage of total 1 76.77189351055192 45.975184598818416 2 14.166141645434768 16.96691191060322 3 4.487806193803601 8.062627171315597 4 1.8597558239345315 4.454891674769793 5 0.8948759670351948 2.6795019567828193 6 0.5120663518939796 1.8399190624574169 7 0.29758435037798914 1.247467839037595 8 0.19550799967197074 0.9366465735775197 9 0.13655776848592943 0.7360039585727237 >10 0.6132810660905536 6.359702349246511 >50 0.034789281552151594 1.4273009504920524 >100 0.02483112885015412 2.933610777805598 >500 0.0022587103202570866 0.9610029575542816 >1k 0.0022285789520366233 2.6033966376657753 >5k 2.4092745426164736E-4 0.8637169444894128 >10k+ 1.8069559069623553E-4 1.952114636811385 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 27952 0.5016647077119283 No Hit GGTATCAACGCAGAGTACTTTTTTT 19603 0.3518221689065874 No Hit TATCAACGCAGAGTACTTTTTTTTT 17557 0.31510186295429043 No Hit CCCATGTACTCTGCGTTGATACCAC 17274 0.3100227590517977 No Hit GTACATGGGAAGCAGTGGTATCAAC 15035 0.2698386119221824 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 10819 0.19417252692957043 No Hit GAGTACATGGGAAGCAGTGGTATCA 7144 0.12821596565161764 No Hit ATTCCATTCCATTCCATTCCATTCC 6622 0.11884744184560637 No Hit GTACTGGTTCACTATCGGTCAGTCA 6316 0.11335554858001357 No Hit CATGTACTCTGCGTTGATACCACTG 6290 0.11288891712607431 No Hit GAATGGAATGGAATGGAATGGAATG 5644 0.10129492023204505 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.794736361304838E-5 0.0 5 0.0 0.0 0.0 5.3842090839145136E-5 0.0 6 0.0 0.0 0.0 5.3842090839145136E-5 0.0 7 0.0 0.0 0.0 1.0768418167829027E-4 0.0 8 0.0 0.0 0.0 1.2563154529133866E-4 0.0 9 0.0 0.0 0.0 1.6152627251743543E-4 0.0 10 1.794736361304838E-5 0.0 0.0 2.1536836335658054E-4 0.0 11 1.794736361304838E-5 0.0 0.0 2.3331572696962894E-4 0.0 12 1.794736361304838E-5 0.0 0.0 2.3331572696962894E-4 0.0 13 1.794736361304838E-5 0.0 0.0 2.692104541957257E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGACCG 85 5.2051844E-5 11.202161 9 TAGCCCG 60 0.005776555 11.10951 5 AATCGCC 190 0.0 10.978583 18 GTCCTAG 435 0.0 10.929077 1 GACCGTC 95 1.6068429E-4 10.023619 7 CGCCCTA 970 0.0 9.774747 16 GCTAGAC 255 0.0 9.68506 3 TAGACCG 90 0.0010906744 9.522438 5 TATACCG 140 1.0265612E-6 9.522438 5 TACACCG 190 1.0095391E-9 9.522438 5 ATCGCCG 190 1.0913936E-9 9.481674 19 ATACCGA 145 1.6547601E-6 9.194078 6 GTACTGG 2095 0.0 9.167906 1 TGTCCCG 1045 0.0 9.079158 11 ACCGACC 105 4.3913003E-4 9.068988 8 GACTCGC 95 0.001786238 9.021256 6 TACCGGT 95 0.001786238 9.021256 7 GTCCCGC 1080 0.0 8.866723 12 AAATCGC 215 1.0822987E-9 8.820004 17 CGGGGGA 495 0.0 8.811094 17 >>END_MODULE