FastQCFastQC Report
Wed 25 May 2016
SRR1294588_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294588_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4334787
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC169110.39012297490049685No Hit
CCCATGTACTCTGCGTTGATACCAC165210.38112599304187267No Hit
GTATCAACGCAGAGTACTTTTTTTT103940.23978110112446127No Hit
GAGTACATGGGAAGCAGTGGTATCA72270.1667209945955822No Hit
GGTATCAACGCAGAGTACTTTTTTT61860.14270597378833147No Hit
TATCAACGCAGAGTACTTTTTTTTT60650.13991460249373266No Hit
CATGTACTCTGCGTTGATACCACTG59710.13774609917396172No Hit
ATTCCATTCCATTCCATTCCATTCC56050.1293027777374067No Hit
GCGTTGATACCACTGCTTCCCATGT50550.11661472639832131No Hit
GTATCAACGCAGAGTACATGGGAAG45580.10514934182463867No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG1600.013.0592319
CGTCGGT2700.010.20114919
AGCGTCG2800.010.18331217
TCGCGTT856.615322E-410.05595710
AATCGCC2105.456968E-129.95782218
AAATCGC2251.8189894E-129.71563417
GCGTCGG2950.09.66629818
ACTCGCG800.00452883549.49729258
CGCCCTA4750.09.40426916
GTATTAG6050.09.2637471
GTACTGG14550.09.2054751
CTAAGAC4350.09.1699063
CGCGCGC3850.09.12726910
TGTCGCC1252.7658418E-59.11740110
TGTCGAA6700.09.07864414
TACTGGT12900.09.0557672
CTAATAC4450.08.96384053
CTCGCGT850.00745664448.9386289
TAGACAG6600.08.9215975
TCGCGCG3750.08.86414059
Produced by FastQC (version 0.11.5)