##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1294588_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4334787 Sequences flagged as poor quality 0 Sequence length 25 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.888230032986627 31.0 30.0 33.0 25.0 34.0 2 30.08203332712772 31.0 30.0 34.0 25.0 34.0 3 30.005970074192803 31.0 30.0 34.0 25.0 34.0 4 33.67220949956711 35.0 35.0 37.0 28.0 37.0 5 33.99806311129013 35.0 35.0 37.0 28.0 37.0 6 34.09576364421135 36.0 35.0 37.0 29.0 37.0 7 34.024728550676194 37.0 35.0 37.0 29.0 37.0 8 33.90631373583062 37.0 35.0 37.0 28.0 37.0 9 35.00354504154414 38.0 35.0 39.0 27.0 39.0 10 35.143167588165234 38.0 35.0 39.0 27.0 39.0 11 35.355972507991744 39.0 35.0 39.0 28.0 39.0 12 35.14402691527865 38.0 35.0 39.0 27.0 39.0 13 34.981800028467376 38.0 35.0 39.0 27.0 39.0 14 35.87486997631026 39.0 35.0 41.0 27.0 41.0 15 35.844880959548874 39.0 34.0 41.0 27.0 41.0 16 35.954029344463756 39.0 35.0 41.0 27.0 41.0 17 35.94466463980814 39.0 35.0 41.0 27.0 41.0 18 35.84301927637967 39.0 34.0 41.0 27.0 41.0 19 36.1248536548624 39.0 35.0 41.0 27.0 41.0 20 36.23564410431239 39.0 35.0 41.0 27.0 41.0 21 36.273440655792314 39.0 35.0 41.0 27.0 41.0 22 36.28239011513138 39.0 35.0 41.0 27.0 41.0 23 36.26054428971943 39.0 35.0 41.0 27.0 41.0 24 36.2727245883131 39.0 35.0 41.0 27.0 41.0 25 35.03913502555027 38.0 33.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0067112208009483 1101 2 0.9850224855482139 1101 3 1.3572573285490712 1101 4 1.088332418820407 1101 5 0.8393566853943497 1101 6 0.7681201887985551 1101 7 0.8249460386473686 1101 8 0.9020558049107024 1101 9 1.1916526777444787 1101 10 1.1742279068698807 1101 11 0.9777912172235617 1101 12 1.1257514143919494 1101 13 1.1999927664894159 1101 14 1.3518158070610937 1101 15 1.430413400032947 1101 16 1.3961980224870487 1101 17 1.3399677934288547 1101 18 1.2967965077619397 1101 19 1.2207011381802815 1101 20 1.182526315967415 1101 21 1.0534266206248333 1101 22 0.9874796331350524 1101 23 1.024549980172651 1101 24 1.1300748492414812 1101 25 1.075545543148749 1102 1 0.9048313529436349 1102 2 0.8567175375801135 1102 3 1.2179486312638268 1102 4 1.0169041404704515 1102 5 0.8050495640454258 1102 6 0.7059570422740862 1102 7 0.6174624310227586 1102 8 0.7775872710406873 1102 9 1.16735452174553 1102 10 1.0714191902189043 1102 11 0.8165068090614795 1102 12 1.0033599962989115 1102 13 1.0339332960890886 1102 14 1.2820655465630537 1102 15 1.3594687164271662 1102 16 1.1947764228726854 1102 17 1.2228665946306094 1102 18 1.3194602770484138 1102 19 1.004947573468847 1102 20 0.3111097036493007 1102 21 0.5642010847723 1102 22 0.6390663479806378 1102 23 0.8234751301338363 1102 24 0.8622691097431883 1102 25 0.9121116212769849 1103 1 0.6867580406040297 1103 2 0.7128953491577796 1103 3 0.9812881461636884 1103 4 0.8601939141662953 1103 5 0.5933183217205027 1103 6 0.5538166135949965 1103 7 0.4435187942887211 1103 8 0.573653226239756 1103 9 0.6506476294527914 1103 10 0.7598218182061132 1103 11 0.6315182545778413 1103 12 0.8331992359137601 1103 13 0.6936721303836251 1103 14 0.5768271362983199 1103 15 0.9621825810303903 1103 16 0.9349301642475396 1103 17 0.9249659208307435 1103 18 0.9290299095246368 1103 19 0.7564046332564942 1103 20 -0.09139293300545148 1103 21 0.36336744891240613 1103 22 -0.3116273177745228 1103 23 0.3007415984100774 1103 24 0.5390601562673538 1103 25 0.5658875111281034 1104 1 0.2471539264883056 1104 2 0.2749935021758958 1104 3 0.5657036135827447 1104 4 0.4361655155739612 1104 5 0.22893444236851934 1104 6 0.08955157284485438 1104 7 0.19116809007417146 1104 8 0.34538803981649124 1104 9 0.6345612912918028 1104 10 0.5757180146041492 1104 11 0.33492853910045284 1104 12 0.4212601732819863 1104 13 0.38418708487994024 1104 14 0.6480312013451979 1104 15 0.6411366479940241 1104 16 0.5442593778426783 1104 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-1.374993559046601 2207 10 -0.1329117440688563 2207 11 0.21314053047507997 2207 12 0.5218219134197213 2207 13 0.6177844557511847 2207 14 0.8539667078235453 2207 15 0.8748759293876418 2207 16 0.7762789023069416 2207 17 0.781270724124127 2207 18 0.7985606359422732 2207 19 -0.4455878038802936 2207 20 0.23030070835725525 2207 21 -0.32149695544686097 2207 22 0.22441582982722252 2207 23 -0.5151785231920769 2207 24 -1.6819573669668344 2207 25 -0.2460459354522797 2208 1 0.5566971881405145 2208 2 0.20242299569507338 2208 3 0.7171096929959617 2208 4 0.6466111973928719 2208 5 0.5156707132868661 2208 6 0.3953340422141878 2208 7 0.5078666046821994 2208 8 0.5598211370476136 2208 9 -0.0018936967480556177 2208 10 0.11844947223104896 2208 11 0.3654623099332568 2208 12 0.35465523138066857 2208 13 0.6387166267800808 2208 14 0.9335538508680159 2208 15 0.8039879844392885 2208 16 0.6927069955646132 2208 17 0.6612377184441058 2208 18 0.8925030979254913 2208 19 -1.1782747683351147 2208 20 0.032359517787028835 2208 21 -0.1274883699231708 2208 22 0.29352145599746393 2208 23 0.5001408920638397 2208 24 0.6449536375571583 2208 25 0.7012940480304621 2209 1 0.13896575339638417 2209 2 -1.3649376226300625 2209 3 -0.21747590455754562 2209 4 0.04250154012691354 2209 5 0.1356534513087837 2209 6 0.17248506370793848 2209 7 0.1573730037757457 2209 8 0.26896337255994496 2209 9 -2.080419541156779 2209 10 -0.3193663617304807 2209 11 0.015161073985801465 2209 12 0.3410228831728972 2209 13 0.4998492920059334 2209 14 0.6651563952761137 2209 15 0.7563145000283527 2209 16 0.6844864819958545 2209 17 0.4417942900665963 2209 18 0.6404058345480195 2209 19 0.45078131767745333 2209 20 0.40139715783751484 2209 21 0.45488062115687455 2209 22 0.39204579460277245 2209 23 0.47574631872812034 2209 24 0.5460539121781167 2209 25 0.5160962279728807 2210 1 0.15095976514788845 2210 2 0.19068990662640672 2210 3 0.11396514427479687 2210 4 0.26904330604604354 2210 5 0.14338167959717651 2210 6 0.14370230319307353 2210 7 0.10266241478230853 2210 8 0.23807090615574822 2210 9 0.34323328682412324 2210 10 -1.571763620929886 2210 11 -0.4837501301795939 2210 12 -2.287354771940386 2210 13 -0.7610678190324691 2210 14 -0.4955323577206201 2210 15 -0.6796612365985908 2210 16 -0.12198629530489313 2210 17 0.04187784911575676 2210 18 0.39088226952789284 2210 19 0.2981577914058562 2210 20 0.340035768512017 2210 21 0.2624915108229757 2210 22 0.18640694633447197 2210 23 0.17034738155786755 2210 24 0.19725906416228156 2210 25 0.038640183726009525 2211 1 -0.3108595409518635 2211 2 -0.03249615612525503 2211 3 -1.485443410253236 2211 4 -0.6417983399937626 2211 5 -0.5942273700823861 2211 6 -0.5243002576084237 2211 7 -0.5346691301141178 2211 8 -0.11472754710197108 2211 9 0.04993558950789634 2211 10 0.032639391719605726 2211 11 -0.22951073505571173 2211 12 -0.21077066682700973 2211 13 -0.2746489855573202 2211 14 -0.8607156652657864 2211 15 -0.41519370687616686 2211 16 -0.10998278211932444 2211 17 -0.19021206940850988 2211 18 -0.08715959248183225 2211 19 -0.05297575340359373 2211 20 -0.19212784353550916 2211 21 -0.2686951726885596 2211 22 -0.27123420221559513 2211 23 -0.581171646315596 2211 24 -0.2948255058863509 2211 25 -0.21793160372639875 2212 1 -0.3133271281340413 2212 2 -0.34581574624197486 2212 3 -2.4091089348271346 2212 4 -1.457791983070507 2212 5 -0.6952974127134937 2212 6 -0.5074436005700917 2212 7 -0.35663431771797605 2212 8 -0.2686376019204033 2212 9 -0.9194696172977785 2212 10 -0.2093567289464744 2212 11 -0.31136857777483584 2212 12 0.05371659875928003 2212 13 -0.4619873580700755 2212 14 -0.0695411138579729 2212 15 0.10423133825258901 2212 16 -1.1057439769160666 2212 17 -1.3069372118597897 2212 18 -0.2645847210340193 2212 19 -0.12068795526405296 2212 20 -0.24136002321515093 2212 21 -0.09163459587470157 2212 22 -0.3797463510776353 2212 23 -0.09623682123274335 2212 24 -0.1372534221956201 2212 25 0.07185705065797521 2213 1 -3.117176539214338 2213 2 -1.4636274360171733 2213 3 -2.3979925727931253 2213 4 -1.103822213111357 2213 5 -0.9073584749508186 2213 6 -0.7409890271523878 2213 7 -0.49203355206028476 2213 8 -0.3204790141800018 2213 9 -0.3657290557260282 2213 10 -0.34805807328987726 2213 11 -0.5857408784507783 2213 12 -0.335968858672139 2213 13 -0.689171771239188 2213 14 -0.3614941177638187 2213 15 -0.46563646676541026 2213 16 -1.7948329198731443 2213 17 -1.0746243612836466 2213 18 -0.34994491109816295 2213 19 -0.31538108870530834 2213 20 -0.2770841296605724 2213 21 -0.023970497628852172 2213 22 -0.13152865949166426 2213 23 0.03537492202874404 2213 24 0.10129849200905738 2213 25 0.1117065276746061 2214 1 -0.9732351415392699 2214 2 -0.642830280809136 2214 3 -6.364084690156009 2214 4 -2.0406890591497095 2214 5 -1.1895302031570623 2214 6 -0.6985073928697929 2214 7 -0.5094687357296337 2214 8 -0.45356265327897205 2214 9 -0.17959260868520488 2214 10 0.02919731470739606 2214 11 -0.7182898410182474 2214 12 -0.4373295132225934 2214 13 -5.1751144128717215 2214 14 -3.882470259219552 2214 15 -2.160615540406596 2214 16 -2.690957602873951 2214 17 -3.2199459492403903 2214 18 -1.0833411523261063 2214 19 -0.6689886870701542 2214 20 -0.3870963136275236 2214 21 -0.31461117832432706 2214 22 -0.23034674775300346 2214 23 -0.20427530926579607 2214 24 -0.13936771902674394 2214 25 0.0606018459761799 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1630.0 3 200.0 4 214.0 5 425.0 6 80.0 7 73.0 8 113.0 9 303.0 10 898.0 11 2290.0 12 5108.0 13 9353.0 14 14930.0 15 21017.0 16 26598.0 17 30103.0 18 31448.0 19 32134.0 20 32493.0 21 32784.0 22 33047.0 23 34361.0 24 37186.0 25 40226.0 26 44740.0 27 51083.0 28 59148.0 29 72665.0 30 90188.0 31 112641.0 32 146288.0 33 192427.0 34 257958.0 35 350676.0 36 487969.0 37 725851.0 38 1356139.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.43325943179795 21.618727654330165 12.966521242053814 22.98149167181807 2 14.578205466728955 22.344672958285862 40.8148483936429 22.26227318134229 3 17.789457721269237 28.55106046228848 30.025300047110115 23.63418176933217 4 11.723711652391957 17.883263737004352 39.075045861565 31.317978749038684 5 12.659781232163345 38.8948884546224 35.10592996871942 13.339400344494834 6 29.653007258880848 39.596808354695995 18.003432646150046 12.74675174027311 7 24.551890657857985 34.242178717432004 24.62476541346094 16.581165211249075 8 23.111003904451167 38.251202901042994 22.14600546830908 16.491787726196755 9 24.36530142887251 17.287951547743234 22.450904509377505 35.89584251400675 10 14.691187374365539 30.646360167054016 33.62982374010045 21.032628718480005 11 31.34996826129609 27.405828380171965 23.19635293438744 18.0478504241445 12 20.05345100124833 28.62774780065149 32.51344815742239 18.80535304067779 13 25.781508601407417 25.157091157883364 27.334646292713305 21.726753947995913 14 19.63330143204911 23.97977061545472 28.87322448116555 27.513703471330626 15 22.92943845269723 30.883982071003608 24.471759660773824 21.714819815525335 16 20.97222263899818 31.9795475598178 26.426060495402215 20.622169305781803 17 19.607013934703613 30.808146328468982 29.383420643564197 20.20141909326321 18 20.077114111790053 30.295265267462142 29.788017049195474 19.83960357155233 19 20.479248766639206 29.480872317522504 29.9776562647526 20.062222651085687 20 22.06110234068441 28.736903896000772 28.754285730114365 20.447708033200456 21 22.07608443998483 29.66495414699139 28.2680006730971 19.990960739926685 22 20.840601931075113 29.1968288946122 29.251792074360267 20.710777099952423 23 20.597408383338585 29.70758614003317 29.079268567154777 20.61573690947347 24 20.777629333819068 28.80417656196866 29.00724235680987 21.410951747402404 25 21.399436965662755 28.469246067243244 28.325890461081034 21.80542650601297 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4736.0 1 4736.0 2 4779.5 3 4823.0 4 4823.0 5 4823.0 6 7501.5 7 10180.0 8 10180.0 9 10180.0 10 15387.5 11 20595.0 12 20595.0 13 20595.0 14 33683.5 15 46772.0 16 46772.0 17 46772.0 18 72570.5 19 98369.0 20 98369.0 21 98369.0 22 138964.5 23 179560.0 24 179560.0 25 179560.0 26 231712.5 27 283865.0 28 283865.0 29 283865.0 30 339622.5 31 395380.0 32 395380.0 33 395380.0 34 438090.0 35 480800.0 36 480800.0 37 480800.0 38 504354.5 39 527909.0 40 527909.0 41 527909.0 42 518398.0 43 508887.0 44 508887.0 45 508887.0 46 518469.5 47 528052.0 48 528052.0 49 528052.0 50 480834.5 51 433617.0 52 433617.0 53 433617.0 54 374794.0 55 315971.0 56 315971.0 57 315971.0 58 267057.5 59 218144.0 60 218144.0 61 218144.0 62 181117.5 63 144091.0 64 144091.0 65 144091.0 66 109633.0 67 75175.0 68 75175.0 69 75175.0 70 55573.5 71 35972.0 72 35972.0 73 35972.0 74 25237.5 75 14503.0 76 14503.0 77 14503.0 78 9785.0 79 5067.0 80 5067.0 81 5067.0 82 3374.0 83 1681.0 84 1681.0 85 1681.0 86 1062.5 87 444.0 88 444.0 89 444.0 90 285.5 91 127.0 92 127.0 93 127.0 94 80.5 95 34.0 96 34.0 97 34.0 98 33.5 99 33.0 100 33.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09804403307475086 2 0.05192873375323863 3 0.054996935258872004 4 0.050360029224042605 5 0.04766093466645535 6 0.06579331348922104 7 0.06221758993002424 8 0.05887255821335627 9 0.06390164038048467 10 0.06401698630174908 11 0.05852652044956304 12 0.059864533136230226 13 0.05972611803071292 14 0.06166392950795506 15 0.06482440775059997 16 0.059749187214965804 17 0.06286352708910495 18 0.14157558375994023 19 0.06745429475542858 20 0.061779275429219474 21 0.05938008026691968 22 0.06450143917105962 23 0.06325570322140396 24 0.07349842102968382 25 0.06694677270186516 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 4334787.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.12177593091452 #Duplication Level Percentage of deduplicated Percentage of total 1 83.51905568921177 57.729854533112665 2 11.274958190598586 15.586902673619695 3 2.746151017560549 5.694565059248195 4 0.956603846373438 2.64488626894703 5 0.44144991542372997 1.5256901069320115 6 0.24746918900878062 1.026330589948204 7 0.16233265093503435 0.7854504786941965 8 0.10466612948643167 0.5787766999933772 9 0.08423007534962515 0.5239919155464752 >10 0.41482858532764877 5.05693971478559 >50 0.02570075237157078 1.2284771711018196 >100 0.018467670726801975 2.4593344507405335 >500 0.001924945685552286 0.9242232619003166 >1k 0.0018574032300833203 2.368646959973614 >5k 2.026258069181491E-4 0.8428957345219802 >10k+ 1.0131290345907456E-4 1.0230343809344016 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 16911 0.39012297490049685 No Hit CCCATGTACTCTGCGTTGATACCAC 16521 0.38112599304187267 No Hit GTATCAACGCAGAGTACTTTTTTTT 10394 0.23978110112446127 No Hit GAGTACATGGGAAGCAGTGGTATCA 7227 0.1667209945955822 No Hit GGTATCAACGCAGAGTACTTTTTTT 6186 0.14270597378833147 No Hit TATCAACGCAGAGTACTTTTTTTTT 6065 0.13991460249373266 No Hit CATGTACTCTGCGTTGATACCACTG 5971 0.13774609917396172 No Hit ATTCCATTCCATTCCATTCCATTCC 5605 0.1293027777374067 No Hit GCGTTGATACCACTGCTTCCCATGT 5055 0.11661472639832131 No Hit GTATCAACGCAGAGTACATGGGAAG 4558 0.10514934182463867 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.6138368505765105E-5 0.0 3 0.0 0.0 0.0 6.920755275864765E-5 0.0 4 0.0 0.0 0.0 6.920755275864765E-5 0.0 5 0.0 0.0 0.0 6.920755275864765E-5 0.0 6 0.0 0.0 0.0 6.920755275864765E-5 0.0 7 0.0 0.0 0.0 1.1534592126441277E-4 0.0 8 0.0 0.0 0.0 1.384151055172953E-4 0.0 9 2.3069184252882553E-5 0.0 0.0 1.384151055172953E-4 0.0 10 2.3069184252882553E-5 0.0 0.0 1.384151055172953E-4 0.0 11 2.3069184252882553E-5 0.0 0.0 1.6148428977017786E-4 0.0 12 2.3069184252882553E-5 0.0 0.0 1.8455347402306042E-4 0.0 13 2.3069184252882553E-5 0.0 0.0 2.0762265827594298E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCGCCG 160 0.0 13.05923 19 CGTCGGT 270 0.0 10.201149 19 AGCGTCG 280 0.0 10.183312 17 TCGCGTT 85 6.615322E-4 10.055957 10 AATCGCC 210 5.456968E-12 9.957822 18 AAATCGC 225 1.8189894E-12 9.715634 17 GCGTCGG 295 0.0 9.666298 18 ACTCGCG 80 0.0045288354 9.4972925 8 CGCCCTA 475 0.0 9.404269 16 GTATTAG 605 0.0 9.263747 1 GTACTGG 1455 0.0 9.205475 1 CTAAGAC 435 0.0 9.169906 3 CGCGCGC 385 0.0 9.127269 10 TGTCGCC 125 2.7658418E-5 9.117401 10 TGTCGAA 670 0.0 9.078644 14 TACTGGT 1290 0.0 9.055767 2 CTAATAC 445 0.0 8.9638405 3 CTCGCGT 85 0.0074566444 8.938628 9 TAGACAG 660 0.0 8.921597 5 TCGCGCG 375 0.0 8.8641405 9 >>END_MODULE