FastQCFastQC Report
Wed 25 May 2016
SRR1294588_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294588_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4334787
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT285940.6596402545269238No Hit
GGTATCAACGCAGAGTACTTTTTTT191530.44184408599545955No Hit
CCCATGTACTCTGCGTTGATACCAC187720.43305472679511126No Hit
TATCAACGCAGAGTACTTTTTTTTT185020.42682604704683297No Hit
GTACATGGGAAGCAGTGGTATCAAC162500.37487424410934145No Hit
ACGCAGAGTACTTTTTTTTTTTTTT111730.25775199565745677No Hit
GAGTACATGGGAAGCAGTGGTATCA77080.1778172722212187No Hit
GTACTTTTTTTTTTTTTTTTTTTTT72470.16718237828063984No Hit
CATGTACTCTGCGTTGATACCACTG69270.15980023931971743No Hit
GAGTACTTTTTTTTTTTTTTTTTTT60950.14060667802131915No Hit
GCGTTGATACCACTGCTTCCCATGT54560.12586546928372722No Hit
ATTCCATTCCATTCCATTCCATTCC52370.12081331793234593No Hit
ACGCAGAGTACATGGGAAGCAGTGG49040.11313127957613604No Hit
GAATGGAATGGAATGGAATGGAATG45600.10519548019314444No Hit
GTATCAACGCAGAGTACATGGGAAG45190.10424964363877624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCCC801.9543477E-613.0904138
CTAGTAC2400.011.4776453
TGCACCG1002.3451856E-510.472335
ATCGCCG1856.184564E-1110.25232519
CGTGCGG750.002604204710.15428210
ACCGCTC856.469786E-410.08031758
AGGCGTT900.00109281279.52037
AGACCGT900.00109281279.52036
CGAATTA800.00458558939.48296315
TAGACAG4750.09.2196595
TGTACCG1451.6594531E-69.1920145
ACCGGGC1054.4006604E-49.0669538
CTGTTCG1054.4037183E-49.0663239
CCGAATT850.00754741678.9254514
GTCCTAG2800.08.8297471
AATCGCC2151.0786607E-98.82156418
GAAATCG2607.2759576E-128.75370616
CGCGCCT3800.08.73461112
AGGACCG1201.7247132E-48.7269425
TACACCG1201.7247132E-48.7269425
Produced by FastQC (version 0.11.5)