##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1294588_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4334787
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.221199103900606	31.0	31.0	34.0	28.0	34.0
2	31.450728259543087	31.0	31.0	34.0	28.0	34.0
3	31.527107790994112	31.0	31.0	34.0	28.0	34.0
4	35.14684550821067	37.0	35.0	37.0	32.0	37.0
5	35.33202115813303	37.0	35.0	37.0	32.0	37.0
6	34.85216759208699	37.0	35.0	37.0	32.0	37.0
7	34.712754282967076	37.0	35.0	37.0	32.0	37.0
8	34.721160693708825	37.0	35.0	37.0	32.0	37.0
9	36.20515471694457	39.0	35.0	39.0	31.0	39.0
10	35.96519690586873	39.0	35.0	39.0	30.0	39.0
11	36.149462476472316	39.0	35.0	39.0	31.0	39.0
12	36.1147518897699	39.0	35.0	39.0	30.0	39.0
13	35.96790084495501	39.0	35.0	39.0	30.0	39.0
14	37.03889464465036	40.0	36.0	41.0	30.0	41.0
15	37.077647413817566	40.0	36.0	41.0	31.0	41.0
16	37.11356959407694	40.0	36.0	41.0	31.0	41.0
17	37.160871572236424	40.0	36.0	41.0	31.0	41.0
18	37.17473661335609	40.0	36.0	41.0	31.0	41.0
19	37.18663639066925	40.0	37.0	41.0	30.0	41.0
20	37.247396930921866	40.0	37.0	41.0	31.0	41.0
21	37.09647925030688	40.0	37.0	41.0	30.0	41.0
22	37.08394645457781	40.0	36.0	41.0	30.0	41.0
23	37.09674616076868	40.0	36.0	41.0	30.0	41.0
24	37.022846335933	40.0	36.0	41.0	30.0	41.0
25	35.93577839003393	39.0	34.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.5644575433718195
1101	2	0.5413947746281949
1101	3	0.45552609617485373
1101	4	0.4186792895233893
1101	5	0.43699462319145255
1101	6	0.48872239423592134
1101	7	0.5856761352711999
1101	8	0.6430081190668062
1101	9	0.8214627233354648
1101	10	0.8496952042359283
1101	11	0.8409304674191134
1101	12	0.8618669827142753
1101	13	0.8685000496377739
1101	14	1.0883685222276185
1101	15	1.0079396552207314
1101	16	1.1233504349985566
1101	17	1.084963183038603
1101	18	1.0750936484730929
1101	19	1.169714583333473
1101	20	1.1074062716089017
1101	21	1.409462645879806
1101	22	1.3018475954340687
1101	23	1.222762161886891
1101	24	1.395986431302859
1101	25	1.341357558159288
1102	1	0.36712557777876853
1102	2	0.41116341340984164
1102	3	0.3436037317680878
1102	4	0.31816354480664444
1102	5	0.33632129235795816
1102	6	0.4153319771040458
1102	7	0.4491750108788537
1102	8	0.4968065283833809
1102	9	0.6042602531005699
1102	10	0.7550344488196359
1102	11	0.6840409308982913
1102	12	0.6794929831099452
1102	13	0.7082439115533177
1102	14	0.9014080827756246
1102	15	0.8680750704663112
1102	16	0.8546679545813589
1102	17	0.8377591868410477
1102	18	0.8342905042535662
1102	19	0.9262062633801804
1102	20	0.923633926299587
1102	21	1.1815288241131725
1102	22	1.1563813014211206
1102	23	1.036126288213758
1102	24	1.0405905256050971
1102	25	0.9850858433823149
1103	1	0.28448910480225464
1103	2	0.31986858963251663
1103	3	0.2549733831163543
1103	4	0.20804633494562808
1103	5	0.24197605503243125
1103	6	0.35015756626236083
1103	7	0.36959170023655474
1103	8	0.3907955319092906
1103	9	0.23551724560207532
1103	10	0.5663715802560176
1103	11	0.5569976278387969
1103	12	0.5433980016173265
1103	13	0.6776164944966467
1103	14	0.7951840406074737
1103	15	0.8312666386095628
1103	16	0.7304645169288833
1103	17	0.7572622447546422
1103	18	0.7022178981746308
1103	19	0.8799489830689566
1103	20	0.7835832791784654
1103	21	1.0548842871443327
1103	22	0.9631567485846873
1103	23	0.9515903154350127
1103	24	1.121300070032973
1103	25	0.9907434213456838
1104	1	0.17958126222123383
1104	2	0.1978073532510365
1104	3	0.10939456245530721
1104	4	0.0923209211189473
1104	5	0.1067218170945452
1104	6	0.11044898918211032
1104	7	0.23719744742467697
1104	8	0.18144983425002437
1104	9	-0.05223106957323154
1104	10	0.3273432583337481
1104	11	0.32202630244336916
1104	12	0.3997259654903971
1104	13	-0.005249854244816277
1104	14	0.28298123294966615
1104	15	0.4962832376787887
1104	16	0.5805944726902581
1104	17	0.5526835084306683
1104	18	0.5395692540847392
1104	19	0.5664682770909977
1104	20	0.5310646675200132
1104	21	0.7504847670989463
1104	22	0.7249894315002976
1104	23	0.6636905768799366
1104	24	0.745522635566715
1104	25	0.6739653275168536
1105	1	0.04360604603018814
1105	2	0.053014234471756794
1105	3	0.028172299070863716
1105	4	0.0012879801180361028
1105	5	0.08396294093556378
1105	6	0.014585334725694565
1105	7	-0.019749296269694128
1105	8	-0.06648782451117796
1105	9	0.201569784547452
1105	10	0.3187084493799546
1105	11	0.26453023069836235
1105	12	-0.07974485780891172
1105	13	-0.32657502783237646
1105	14	-0.7260276162469523
1105	15	-0.12447119119224226
1105	16	0.07607982301893657
1105	17	0.2611650856779377
1105	18	-0.540371073921456
1105	19	-0.4712087743209068
1105	20	-0.48800438643501565
1105	21	0.18202379263711066
1105	22	0.37478101976000033
1105	23	0.3457572397591022
1105	24	0.4256287953982181
1105	25	0.09910521064102795
1106	1	-0.4765405205196238
1106	2	-0.4891747231555321
1106	3	-0.6575295765986517
1106	4	-0.5027850816997415
1106	5	-0.47784509769020644
1106	6	-0.46743580142714336
1106	7	-0.4704623493578737
1106	8	-0.44362308615701096
1106	9	-0.34390285819085875
1106	10	-1.5017249725228226
1106	11	-1.1447041999067977
1106	12	-1.7453515431063948
1106	13	-0.7667223504464857
1106	14	-0.9830987277137524
1106	15	-0.842135249437078
1106	16	-0.8792856898319457
1106	17	-1.2138606627352289
1106	18	-0.7245689480134274
1106	19	-0.45874668310335665
1106	20	-0.6098538169000918
1106	21	-0.3272386820551816
1106	22	-0.43988347402612504
1106	23	-0.7517034197622223
1106	24	-0.4933512738620962
1106	25	-0.9644342353381106
1107	1	0.10128632474907917
1107	2	0.13439848762698148
1107	3	0.04097438644019391
1107	4	-0.04055911365981757
1107	5	0.044613823024526766
1107	6	0.0334896558936606
1107	7	0.1099874752779968
1107	8	0.12929247513064013
1107	9	0.18827897631656043
1107	10	0.25043272730929544
1107	11	0.32144977496810867
1107	12	-0.0700469346622512
1107	13	0.08401951192328738
1107	14	0.19998468792780244
1107	15	0.2874059876622965
1107	16	0.1599600267393555
1107	17	0.24048208230519208
1107	18	0.21120683078560631
1107	19	0.33486579074167366
1107	20	0.28260777032893714
1107	21	0.5224838508068856
1107	22	0.48870189932669206
1107	23	0.5300814986506026
1107	24	0.6012229137353486
1107	25	0.5148183107748991
1108	1	0.0845910512688377
1108	2	0.10411113061728727
1108	3	0.05297890783928594
1108	4	0.05074925948571263
1108	5	0.08694425366393688
1108	6	0.10472864153766182
1108	7	0.07834118267137313
1108	8	0.08764206187271384
1108	9	0.14177505298591342
1108	10	-0.7567719396030981
1108	11	-0.034065013740836037
1108	12	0.07350663164705651
1108	13	0.212550328345543
1108	14	0.29503470364588225
1108	15	0.018084704771077043
1108	16	0.06518948416088222
1108	17	0.17553492654383973
1108	18	0.2232669211337992
1108	19	0.26028371198422917
1108	20	0.2908137367812529
1108	21	0.4395185548979583
1108	22	0.3453401985537141
1108	23	0.2944372379541562
1108	24	0.4374269023401709
1108	25	0.32836547505275604
1109	1	-0.016857025315339058
1109	2	-0.012694344666083879
1109	3	-0.047200188327533255
1109	4	-0.08108264402664389
1109	5	-0.008723902396774008
1109	6	-0.004193250158365913
1109	7	-0.2784359641030747
1109	8	-0.20735518699584077
1109	9	0.06576697905122586
1109	10	-0.3572160517846399
1109	11	0.019401566709802864
1109	12	0.08524481494174552
1109	13	0.3035514965162065
1109	14	0.1671202174545101
1109	15	-3.2896866660409216
1109	16	-1.5573262414465034
1109	17	-1.4135465617408656
1109	18	-0.666400779613646
1109	19	-0.3324542703102509
1109	20	-0.3443496677718514
1109	21	0.2465229746138604
1109	22	0.2935904399087903
1109	23	0.2345592893733155
1109	24	0.3261260728811095
1109	25	0.21240383714742705
1110	1	-0.1847727874345999
1110	2	-0.19300700846363483
1110	3	-0.20843879117367692
1110	4	-0.1715669583354824
1110	5	-0.10887555364823953
1110	6	-0.9390696670116796
1110	7	-1.0118468922311123
1110	8	-0.966003310476566
1110	9	-0.39025067228229204
1110	10	-0.1553277268940647
1110	11	-0.10406831490984558
1110	12	-0.025833845466465277
1110	13	-0.0065764316138370305
1110	14	0.05896118790776228
1110	15	0.015318470765095071
1110	16	-0.029254364032127
1110	17	-0.13796026153507768
1110	18	-0.2655798804301668
1110	19	0.08999223790964095
1110	20	-0.010565880111144565
1110	21	-1.0954042930328143
1110	22	-1.0827688771613921
1110	23	-1.2973945416282007
1110	24	-1.172276195403903
1110	25	-0.7478833879129567
1111	1	-0.36076527942005043
1111	2	-0.3192300167392901
1111	3	-0.33861996933847394
1111	4	-0.13740459000309357
1111	5	-0.24675870404564648
1111	6	-0.2248530839401468
1111	7	-0.4216970377681548
1111	8	-0.34005225243308956
1111	9	-0.2781865428282231
1111	10	-0.20133981234830856
1111	11	-0.5488634094791678
1111	12	-0.47063917695783175
1111	13	-0.23388920502763
1111	14	-0.3914605348919977
1111	15	-0.6136323573315181
1111	16	-0.7530270498511413
1111	17	-0.7477033288675372
1111	18	-1.172586944802113
1111	19	-0.5740455445222352
1111	20	-0.49398945617768675
1111	21	-0.11446620964822074
1111	22	-0.20973278266998818
1111	23	-1.352030722058558
1111	24	-1.1196078712942494
1111	25	-1.6320561339250688
1112	1	-0.24553342128481148
1112	2	-0.42154216458086324
1112	3	-0.36706703849710465
1112	4	-0.2328382552513375
1112	5	-0.20520298789247704
1112	6	-0.17975802140116315
1112	7	-0.3416394124982034
1112	8	-0.35434656410761534
1112	9	-0.5410209561267934
1112	10	-0.28820215945918903
1112	11	-0.13902149414756337
1112	12	-0.45582574283791644
1112	13	-0.18293310394878404
1112	14	-0.9195880984426239
1112	15	-0.482439289452131
1112	16	-0.41761203924135515
1112	17	-0.3913439263381093
1112	18	-0.30360945259648986
1112	19	-1.1979635915965474
1112	20	-0.8977278430125679
1112	21	-0.8226459905901464
1112	22	-0.9829123322089544
1112	23	-0.5470352322971692
1112	24	-1.030783583104153
1112	25	-0.9202711966132284
1113	1	-0.05331019207653753
1113	2	-0.04869510413534073
1113	3	-0.22886543448321817
1113	4	-0.013800136436444177
1113	5	-0.09902827108196277
1113	6	0.1306569852591366
1113	7	0.03053992833819308
1113	8	0.09106120779720328
1113	9	-0.2519847363929699
1113	10	0.07586154964214131
1113	11	0.34838056970075826
1113	12	0.11892539982091677
1113	13	0.19881449668731221
1113	14	0.16310924547961037
1113	15	0.32380956586154497
1113	16	-0.23563898186274912
1113	17	-0.1432560847147073
1113	18	0.0053403555692881355
1113	19	-0.0775488755429592
1113	20	-0.14358368991825188
1113	21	-10.49410577568986
1113	22	-2.6407591527349794
1113	23	-1.874629150642086
1113	24	-0.8636065975337957
1113	25	-1.41306421772682
1201	1	0.5067538644851588
1201	2	0.5134456893194752
1201	3	0.49394335819673785
1201	4	0.3932571411734713
1201	5	0.3725049656701813
1201	6	0.49601088707721885
1201	7	0.6601260700057736
1201	8	0.5326923631247169
1201	9	0.8218405888360891
1201	10	0.8868902978851025
1201	11	0.8329209007929421
1201	12	0.8441085232056338
1201	13	0.8816222346262066
1201	14	1.0943593445770077
1201	15	1.0610576257928912
1201	16	1.0900649716238604
1201	17	1.0626874501017411
1201	18	1.0092163135395822
1201	19	1.1897092819829567
1201	20	1.1616253263254492
1201	21	1.400509865982201
1201	22	1.2911457216853393
1201	23	1.2600047973364283
1201	24	1.3816091952488847
1201	25	1.3813810108796858
1202	1	0.3911745395761699
1202	2	0.4014147815381399
1202	3	0.4269228869363708
1202	4	0.31591731482149754
1202	5	0.3441556631531668
1202	6	0.39435408937423233
1202	7	0.4762328557043034
1202	8	0.4999303231523484
1202	9	0.607583223249172
1202	10	0.7749063054503509
1202	11	0.6857157937573533
1202	12	0.7219175030234553
1202	13	0.7781454767093976
1202	14	0.8484992870536487
1202	15	0.8363861557133703
1202	16	0.8470781422193312
1202	17	0.8060070967299637
1202	18	0.8289714105306274
1202	19	0.9053680444957592
1202	20	0.8457863745833407
1202	21	1.093424239962438
1202	22	1.095754388897518
1202	23	1.0111670852374175
1202	24	1.1346564266309613
1202	25	1.1512924106964348
1203	1	0.3292096660895858
1203	2	0.33193396608054115
1203	3	0.36193306120182456
1203	4	0.26978013282885627
1203	5	0.2360786366355967
1203	6	0.34459009591608947
1203	7	0.36564717203157926
1203	8	0.4491022033154124
1203	9	0.5043521141452842
1203	10	0.5284537018264217
1203	11	0.583056365838523
1203	12	0.4897236914006484
1203	13	0.7177433536803335
1203	14	0.7210109202225041
1203	15	0.7836618196524725
1203	16	0.7775854465867269
1203	17	0.7777838007813713
1203	18	0.6170789225324427
1203	19	0.8233118399251182
1203	20	0.8247969112239772
1203	21	1.046070444470395
1203	22	0.8755524945767448
1203	23	0.8194609547984655
1203	24	1.051878053683616
1203	25	0.8798226658903516
1204	1	0.20234983210781365
1204	2	0.19075012936212588
1204	3	0.1753315808552749
1204	4	0.16577389440980284
1204	5	0.14337068408239872
1204	6	0.1635720707083479
1204	7	0.23679701923659735
1204	8	0.26394919661804295
1204	9	-0.0881738442751896
1204	10	0.29211186016063806
1204	11	0.39067854101308086
1204	12	0.469913981203149
1204	13	0.36244990540433264
1204	14	0.5386518127743827
1204	15	0.5596804140611837
1204	16	0.476720699867613
1204	17	0.5066076107317343
1204	18	0.5693183676236302
1204	19	0.5893711841761515
1204	20	0.5711562187905557
1204	21	0.6438672391946199
1204	22	0.5559739188418362
1204	23	0.6258924457640163
1204	24	0.606390221362858
1204	25	0.5545707937256452
1205	1	0.11487942964173214
1205	2	0.11606619600962276
1205	3	0.1373310969826953
1205	4	0.04040330182216678
1205	5	0.14615062796052314
1205	6	0.12074687580519594
1205	7	0.02563688055803226
1205	8	-0.001901167414089855
1205	9	0.13674515560860812
1205	10	0.16448552392436966
1205	11	-0.10235207183042405
1205	12	-0.0910558139359523
1205	13	0.21204553674646576
1205	14	-0.187077040280073
1205	15	0.1355396060744809
1205	16	0.26526280255384904
1205	17	0.142941773941466
1205	18	0.07954261776171734
1205	19	0.10629390846995079
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2111	21	-0.025657508409793195
2111	22	-0.22181069163684697
2111	23	-1.3321677787374213
2111	24	-1.138216506700111
2111	25	-1.2707643353131957
2112	1	-0.3193349653372728
2112	2	-0.30931475350115534
2112	3	-0.2983442500373883
2112	4	-0.2157748144706062
2112	5	-0.2678837269954215
2112	6	-0.5193814601973159
2112	7	-0.5609907220631385
2112	8	-0.6394823228225519
2112	9	-0.4425812674180136
2112	10	-0.43855974476483084
2112	11	-0.27172289537374184
2112	12	-0.08073018030838597
2112	13	-0.06470201099839556
2112	14	-0.9674704193788486
2112	15	-1.2726186209929722
2112	16	-1.3337677847514442
2112	17	-0.9478171783001841
2112	18	-1.70749273163036
2112	19	-1.4413082239785382
2112	20	-0.9921334646119391
2112	21	-0.7141213352480023
2112	22	-1.1252058899074342
2112	23	-0.7236915754449669
2112	24	-0.40191219229200215
2112	25	-0.4669594824950849
2113	1	-0.19194835789568288
2113	2	-0.3606528018272357
2113	3	-0.1418310129338174
2113	4	-0.09702356732573492
2113	5	-0.12080298510177556
2113	6	0.02638840551049526
2113	7	-0.1590514008526256
2113	8	-0.08854380853486532
2113	9	-0.3656119119677115
2113	10	-0.17062650866487417
2113	11	-2.2270793827061652E-4
2113	12	-0.09078613094605004
2113	13	0.04924532466062459
2113	14	-0.4118920276219882
2113	15	-0.14741262206488415
2113	16	-0.03859611107580463
2113	17	-0.3470344424032561
2113	18	-0.21997264555208318
2113	19	-0.6457471094132856
2113	20	-0.13273894283936727
2113	21	0.08825662281276436
2113	22	-1.9621533912487479
2113	23	-2.4982078236441225
2113	24	-1.3176455513248797
2113	25	-1.7929390276709398
2114	1	-0.7424104373726443
2114	2	-0.8711210667781586
2114	3	-0.6582329855754985
2114	4	-0.48048949772095995
2114	5	-0.4878119162362822
2114	6	-0.3564704128182612
2114	7	-0.6513689854841118
2114	8	-0.554668651623949
2114	9	-0.9822895138656023
2114	10	-0.9470678859416921
2114	11	-0.46465713045528645
2114	12	-1.0464157273757664
2114	13	-0.5592853606535044
2114	14	-1.4559184025963816
2114	15	-0.8051604716203542
2114	16	-4.152404886368501
2114	17	-3.5622669162833347
2114	18	-2.2254459158225615
2114	19	-2.636514450772296
2114	20	-1.6246336329005757
2114	21	-1.9861426738671852
2114	22	-2.582283045209522
2114	23	-3.5297065346886924
2114	24	-7.618395085228862
2114	25	-4.086981456998121
2201	1	0.5603979145836355
2201	2	0.6187158835682887
2201	3	0.6272414075912209
2201	4	0.4501666057390281
2201	5	0.3926123677826183
2201	6	0.5518170341694884
2201	7	0.6302063434777168
2201	8	0.6739820463330375
2201	9	0.8087245076495364
2201	10	0.9962245611089813
2201	11	0.7863233943771419
2201	12	0.925636483385226
2201	13	0.8926982408180422
2201	14	1.141217052874424
2201	15	1.0828075513221194
2201	16	1.101852170669062
2201	17	1.0468971238988232
2201	18	1.0875738540778812
2201	19	1.2101878695742485
2201	20	1.1080637875119734
2201	21	1.4250340124708316
2201	22	1.299758457774928
2201	23	1.2772521157119812
2201	24	1.4713586432058037
2201	25	1.4975334858308145
2202	1	0.40242183958056543
2202	2	0.47470088548158884
2202	3	0.4518624747202189
2202	4	0.3766270500052187
2202	5	0.28138799399005165
2202	6	0.45162433592732043
2202	7	0.5397101531793993
2202	8	0.4882890226895995
2202	9	0.7078007072273138
2202	10	0.8632786014470568
2202	11	0.7981217614749667
2202	12	0.7783397223693811
2202	13	0.8162132476381458
2202	14	1.0604799052710092
2202	15	1.044227097324594
2202	16	1.0421968945274642
2202	17	0.9384973506215744
2202	18	0.9545151798491815
2202	19	1.0735779785796495
2202	20	1.0104759537580748
2202	21	1.2694364637138449
2202	22	1.1261781590710385
2202	23	1.1545196040260564
2202	24	1.323438397606978
2202	25	1.308649401473403
2203	1	0.1559788312182384
2203	2	0.30328667174359225
2203	3	-0.314228763179198
2203	4	-0.05270188468583825
2203	5	0.13240429660180553
2203	6	0.35466804306189914
2203	7	0.41429854354845475
2203	8	0.44615492397278445
2203	9	0.4932249335467844
2203	10	0.7884738008833594
2203	11	0.718842157132066
2203	12	0.7000356602934374
2203	13	0.7476572415508613
2203	14	0.7812878910584189
2203	15	0.7631082704711787
2203	16	0.7895136029934378
2203	17	0.7882445590016758
2203	18	0.7919060282360775
2203	19	0.8763559921303496
2203	20	0.9072612985357651
2203	21	1.1883080572402278
2203	22	1.0331779793357612
2203	23	1.0453387296438805
2203	24	1.1312334437458773
2203	25	1.1460161247138316
2204	1	0.16544541345535535
2204	2	0.2323589686904164
2204	3	0.17607454144025425
2204	4	0.12125305196550329
2204	5	0.051746312997821065
2204	6	0.20066554911817747
2204	7	0.285595072835072
2204	8	0.2569649072597002
2204	9	0.3537592880075522
2204	10	0.49901612246085136
2204	11	0.3415538721694418
2204	12	0.38915798819853364
2204	13	0.4832888662689001
2204	14	0.6551709105115506
2204	15	0.5949507218037908
2204	16	0.6403497642787599
2204	17	0.6618056101587442
2204	18	0.602742856616203
2204	19	0.7306351518351519
2204	20	0.6004874404488376
2204	21	0.9347505351183756
2204	22	0.8340784651513005
2204	23	0.7628227545091164
2204	24	0.9267529861672301
2204	25	0.9699937702401584
2205	1	0.02385770232588058
2205	2	0.08394809637427869
2205	3	-0.09718845970964196
2205	4	-0.02512668406042451
2205	5	-0.07432984055231628
2205	6	0.046470030276275054
2205	7	0.1319513613546377
2205	8	0.1798888311118887
2205	9	0.23458886577788007
2205	10	0.4602904713195457
2205	11	-0.03705460919583459
2205	12	0.32583334195528835
2205	13	0.09144608060753256
2205	14	0.05656706926623656
2205	15	0.23928756476975366
2205	16	0.42780800980237643
2205	17	0.4305165307422385
2205	18	0.4729028572087728
2205	19	0.5598258100638205
2205	20	0.5082160049722262
2205	21	0.8120576181544195
2205	22	0.8018848695389309
2205	23	0.6655564288490652
2205	24	0.7298360217485254
2205	25	0.6003111461773898
2206	1	-0.5419598608608389
2206	2	-0.4366103861341877
2206	3	-0.18555176264396778
2206	4	-0.2499510912655012
2206	5	-0.2337173954038292
2206	6	-0.16059664801455398
2206	7	-0.2385290383761287
2206	8	-0.25736934420791613
2206	9	-0.16939147053359704
2206	10	-0.12861088797285447
2206	11	-0.6036499608439883
2206	12	-0.25064757020082595
2206	13	-1.4279587460074836
2206	14	-0.7303833680473772
2206	15	-0.5670462614202805
2206	16	-0.1609939459955143
2206	17	-0.14588971502181636
2206	18	-0.3075819483917286
2206	19	-0.31568649337779675
2206	20	-0.19539788090931154
2206	21	0.09440487402310538
2206	22	-0.0011104717592900215
2206	23	-0.11059548309491163
2206	24	0.12947953117167543
2206	25	0.216413663881319
2207	1	0.2501593202457215
2207	2	0.26558074994544967
2207	3	0.27841602558729406
2207	4	0.23706578288648927
2207	5	0.1851753340631035
2207	6	0.36595205426986865
2207	7	0.32644915175350064
2207	8	0.31398573461071777
2207	9	0.3849735383026669
2207	10	0.6167712974966761
2207	11	0.486456221030096
2207	12	0.383832099189128
2207	13	0.5434099146185929
2207	14	0.5409285027135198
2207	15	0.485312507993342
2207	16	0.6209377912432785
2207	17	0.6327250824071413
2207	18	0.5989177549104525
2207	19	0.7194334860952623
2207	20	0.5854048685858189
2207	21	0.9367823706596852
2207	22	0.724067251539239
2207	23	0.6535969770865719
2207	24	0.8549024919136556
2207	25	0.8638662914200239
2208	1	0.19144156127074652
2208	2	0.17315530529884526
2208	3	0.2405524355576496
2208	4	0.11114057949330203
2208	5	0.12244293155988117
2208	6	0.27166205578561176
2208	7	0.22265411711556737
2208	8	0.20298741985936175
2208	9	0.39669266988889973
2208	10	0.40953324669640523
2208	11	0.010855540115940698
2208	12	-0.15513759498320923
2208	13	0.06176909151523802
2208	14	-0.3871289646925007
2208	15	0.172363457061941
2208	16	0.25464828928841854
2208	17	0.2364475142992788
2208	18	0.3496875864618616
2208	19	0.49858771495489407
2208	20	0.47790244157038586
2208	21	0.693443495666223
2208	22	0.6960564045317597
2208	23	0.6377151395493428
2208	24	0.6874713602703864
2208	25	0.7336655726109029
2209	1	-0.010237214889475155
2209	2	-0.015067292829215262
2209	3	0.07656718640999216
2209	4	-0.5106068968066424
2209	5	-0.027293776039620354
2209	6	0.09244491213326711
2209	7	0.13449928014124168
2209	8	0.20453233471554455
2209	9	0.23072590853985275
2209	10	0.31798674454382336
2209	11	0.3595375183274214
2209	12	0.30937949188877667
2209	13	0.3201747161296069
2209	14	0.3733197071094665
2209	15	-0.2536119918540862
2209	16	-0.2183378906959419
2209	17	0.1614033666450041
2209	18	-0.24329778585958195
2209	19	0.2292501963081861
2209	20	0.3050513271175461
2209	21	0.6302384353985317
2209	22	0.6081039763964995
2209	23	0.6043819930788956
2209	24	0.7020914994282137
2209	25	0.6665566222576231
2210	1	-0.16950612760541262
2210	2	-0.14883808029345857
2210	3	-0.02860452693450455
2210	4	-0.009486543536198155
2210	5	-0.060590445822249706
2210	6	-0.6079971825953194
2210	7	-0.16874202344473588
2210	8	-0.284357647524331
2210	9	-0.05021672312808789
2210	10	0.14539245378034593
2210	11	0.04403287172003445
2210	12	0.07831678250570207
2210	13	0.1296062280836523
2210	14	0.07940152970235914
2210	15	0.017328642976593756
2210	16	0.07839514826249427
2210	17	-0.8845318504400623
2210	18	-1.3686524212251143
2210	19	-0.32586258924738587
2210	20	-0.16449828063149852
2210	21	-0.4189302229186964
2210	22	-0.2960459190061613
2210	23	-0.16055494888880872
2210	24	0.2683911130798151
2210	25	0.23228566872462153
2211	1	-0.24474011339354007
2211	2	-0.2483015158199109
2211	3	-0.19459539684743277
2211	4	-0.14689416024383206
2211	5	-0.12179277325758875
2211	6	-0.0724703106472191
2211	7	-0.17544435953143278
2211	8	-0.15425891049543594
2211	9	-0.16213530604792936
2211	10	-0.016954291115069964
2211	11	-0.39927919887289676
2211	12	-0.26140281066543025
2211	13	-0.17440306434161812
2211	14	-0.2375231221941121
2211	15	-0.2599611455969537
2211	16	-0.3215993469362033
2211	17	-0.4212509661498487
2211	18	-0.5009622979810331
2211	19	-0.310261688410705
2211	20	-0.4198039370620208
2211	21	-0.07422257162567547
2211	22	-0.21872314170830975
2211	23	-0.8637755582769913
2211	24	-0.2299995141675808
2211	25	-0.9242177830329794
2212	1	-0.23680922956269157
2212	2	-0.33990259478220963
2212	3	-0.2039432233422822
2212	4	-0.12055399095199704
2212	5	-0.1080523566686793
2212	6	-0.19635549287040277
2212	7	-0.2463853946083887
2212	8	-0.4510328484379258
2212	9	-0.2122946404732744
2212	10	-0.6195153183144271
2212	11	-0.29595668027618416
2212	12	-0.4213144886325111
2212	13	-0.4224834810661946
2212	14	-0.42379353044501045
2212	15	-0.2933304173984297
2212	16	-0.4883945405341308
2212	17	-0.7521200319185439
2212	18	-0.5263729743617631
2212	19	-0.5234581351498235
2212	20	-0.6633160404597405
2212	21	-0.17899035066731273
2212	22	-0.5438952819191414
2212	23	-0.3926335997401651
2212	24	-0.5575905153483518
2212	25	-0.3361425131588973
2213	1	-0.25928930335712863
2213	2	-0.2767760281860241
2213	3	-0.19864591991137104
2213	4	-0.0899646766901867
2213	5	-0.12233488661523495
2213	6	-0.34263490092606474
2213	7	-0.44270275586687546
2213	8	-0.38311823044949733
2213	9	-0.37170248617186274
2213	10	-0.4006844580212885
2213	11	-0.15746982070091065
2213	12	0.01617733383160669
2213	13	0.0874252964844544
2213	14	-0.7464868445919279
2213	15	-0.38948949606375294
2213	16	-0.42123997446790895
2213	17	-0.41480422939385875
2213	18	-0.2839363749062258
2213	19	-0.41022558532947784
2213	20	-0.4776328891487296
2213	21	-0.1356086499645528
2213	22	-0.49534601254004684
2213	23	-0.5487038607451424
2213	24	-0.8433335902858516
2213	25	-0.8090152988768011
2214	1	-0.26321169477406414
2214	2	-0.14678280787592257
2214	3	-0.1557865035367776
2214	4	-0.12941017839028035
2214	5	-0.17177668808960078
2214	6	-0.16263869852615187
2214	7	-0.23329645343306993
2214	8	-0.1988557834196314
2214	9	-0.38345694089674254
2214	10	-0.20664325162598374
2214	11	-0.12638628141066022
2214	12	-0.31753096179451745
2214	13	-0.3639304284889704
2214	14	-0.408140882006812
2214	15	-0.16395294811874805
2214	16	-0.45238248610566956
2214	17	-0.4948044180566882
2214	18	-0.3748944960192375
2214	19	-0.596962680031524
2214	20	-0.4775184134153321
2214	21	-0.658020637235289
2214	22	-0.9891106453200251
2214	23	-1.127991421231357
2214	24	-0.5876107773596502
2214	25	-0.9158968362719051
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	17.0
9	54.0
10	135.0
11	315.0
12	757.0
13	1811.0
14	3714.0
15	6416.0
16	9595.0
17	12433.0
18	15365.0
19	16746.0
20	18424.0
21	19900.0
22	21721.0
23	24070.0
24	27078.0
25	30787.0
26	35188.0
27	39508.0
28	47069.0
29	58720.0
30	75130.0
31	97673.0
32	130617.0
33	176155.0
34	240228.0
35	335178.0
36	478570.0
37	759038.0
38	1652371.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.2792347580483	21.705146047080408	12.250618309345631	24.765000885525662
2	14.27525735405223	22.750621887534496	39.56554266680231	23.40857809161096
3	17.372667593700484	28.212241466130155	30.47440416518449	23.940686774984872
4	11.605118916026825	17.909745962244685	39.0356453913767	31.4494897303518
5	12.778824069078915	38.24025237746047	35.40423634912892	13.57668720433169
6	29.94057788611396	39.655372504323125	17.44486063893481	12.959188970628102
7	24.607798934625233	34.26730910958648	24.37959663801947	16.745295317768814
8	23.604024663721106	37.72385040345161	21.854015376639055	16.818109556188233
9	24.446027504958725	16.248498771103137	22.78871232740193	36.516761396536204
10	14.758005588539442	30.169173171768264	33.79185759299082	21.280963646701476
11	31.825460562353864	26.369210263707103	23.289807275600577	18.51552189833845
12	20.34214006177106	28.113537311325587	32.76588362420975	18.778439002693602
13	26.31838657816405	24.221005553444723	27.72182347137241	21.738784397018815
14	19.97426378649924	23.0870629619069	29.53629599690686	27.40237725468701
15	23.130884295863854	29.990516148961287	25.121085777522374	21.757513777652484
16	20.961718268250106	31.264333185767317	26.93844190124396	20.83550664473862
17	19.42670743762378	30.24223808101209	29.97177484004397	20.359279641320157
18	19.930741519284965	29.46147621007406	30.7154741098502	19.892308160790776
19	20.28789083983272	28.667139740811965	31.215119240522217	19.8298501788331
20	21.727397217295657	27.833202915214073	30.186773203505783	20.252626663984483
21	21.7130150609789	28.98692851167926	29.410733375503888	19.88932305183795
22	20.497643534027628	28.523054470825798	30.453651709046298	20.525650286100277
23	20.19798449011531	29.02054635248261	30.365365379220744	20.416103778181334
24	20.422830561877795	28.13855170705082	30.36394414856927	21.07467358250211
25	21.0663689151062	27.78649552103832	29.571782195348707	21.575353368506775
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2353.0
1	2353.0
2	2793.5
3	3234.0
4	3234.0
5	3234.0
6	7878.0
7	12522.0
8	12522.0
9	12522.0
10	16641.0
11	20760.0
12	20760.0
13	20760.0
14	30015.5
15	39271.0
16	39271.0
17	39271.0
18	65206.0
19	91141.0
20	91141.0
21	91141.0
22	134082.5
23	177024.0
24	177024.0
25	177024.0
26	232431.0
27	287838.0
28	287838.0
29	287838.0
30	343893.5
31	399949.0
32	399949.0
33	399949.0
34	442379.5
35	484810.0
36	484810.0
37	484810.0
38	503874.0
39	522938.0
40	522938.0
41	522938.0
42	513735.5
43	504533.0
44	504533.0
45	504533.0
46	517408.5
47	530284.0
48	530284.0
49	530284.0
50	484565.5
51	438847.0
52	438847.0
53	438847.0
54	379187.5
55	319528.0
56	319528.0
57	319528.0
58	269749.0
59	219970.0
60	219970.0
61	219970.0
62	183173.0
63	146376.0
64	146376.0
65	146376.0
66	111109.5
67	75843.0
68	75843.0
69	75843.0
70	56012.5
71	36182.0
72	36182.0
73	36182.0
74	25246.0
75	14310.0
76	14310.0
77	14310.0
78	9615.5
79	4921.0
80	4921.0
81	4921.0
82	3261.5
83	1602.0
84	1602.0
85	1602.0
86	1011.5
87	421.0
88	421.0
89	421.0
90	259.5
91	98.0
92	98.0
93	98.0
94	62.0
95	26.0
96	26.0
97	26.0
98	16.0
99	6.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09287653580210516
2	0.0
3	0.12842614873579716
4	0.011765283968970102
5	0.02216948606702013
6	0.012503497865062344
7	0.02106216522288177
8	0.01868603924483487
9	1.1534592126441275E-4
10	0.39268365435256686
11	0.06334797995841548
12	0.0016379120819546612
13	0.0
14	8.996981858624195E-4
15	0.0011534592126441277
16	9.227673701153021E-5
17	4.6138368505765105E-5
18	2.5376102678170806E-4
19	4.3831450080476846E-4
20	2.5376102678170806E-4
21	2.0762265827594295E-4
22	0.002722163741840141
23	6.920755275864765E-5
24	2.3069184252882553E-5
25	0.0017763271874719564
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	4334787.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.57322101341329
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.51451948134782	54.93257342247364
2	11.965434225246053	15.931549943975298
3	2.9436063102064853	5.87896060397563
4	1.029923088112008	2.7426118948679132
5	0.4560847868133703	1.518151665669099
6	0.26810400266642215	1.0709128214455481
7	0.15704721446577893	0.7318597242719852
8	0.11070972107130371	0.5896242183370591
9	0.08039993079242701	0.4817234125896638
>10	0.4210852100589425	5.018571745126488
>50	0.028370734281565908	1.3158801420006956
>100	0.020136106799571746	2.6177341800568423
>500	0.0021672495498248497	1.0090345984353961
>1k	0.001992471007798602	2.6442241387452334
>5k	2.0973379029458317E-4	0.8998930487056461
>10k+	2.0973379029458317E-4	2.616694439323964
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	28594	0.6596402545269238	No Hit
GGTATCAACGCAGAGTACTTTTTTT	19153	0.44184408599545955	No Hit
CCCATGTACTCTGCGTTGATACCAC	18772	0.43305472679511126	No Hit
TATCAACGCAGAGTACTTTTTTTTT	18502	0.42682604704683297	No Hit
GTACATGGGAAGCAGTGGTATCAAC	16250	0.37487424410934145	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	11173	0.25775199565745677	No Hit
GAGTACATGGGAAGCAGTGGTATCA	7708	0.1778172722212187	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	7247	0.16718237828063984	No Hit
CATGTACTCTGCGTTGATACCACTG	6927	0.15980023931971743	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	6095	0.14060667802131915	No Hit
GCGTTGATACCACTGCTTCCCATGT	5456	0.12586546928372722	No Hit
ATTCCATTCCATTCCATTCCATTCC	5237	0.12081331793234593	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	4904	0.11313127957613604	No Hit
GAATGGAATGGAATGGAATGGAATG	4560	0.10519548019314444	No Hit
GTATCAACGCAGAGTACATGGGAAG	4519	0.10424964363877624	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.3069184252882553E-5	0.0
6	0.0	0.0	0.0	2.3069184252882553E-5	0.0
7	0.0	0.0	0.0	4.6138368505765105E-5	0.0
8	0.0	0.0	0.0	4.6138368505765105E-5	0.0
9	2.3069184252882553E-5	0.0	0.0	6.920755275864765E-5	0.0
10	2.3069184252882553E-5	0.0	0.0	6.920755275864765E-5	0.0
11	2.3069184252882553E-5	0.0	0.0	9.227673701153021E-5	0.0
12	2.3069184252882553E-5	0.0	0.0	9.227673701153021E-5	0.0
13	2.3069184252882553E-5	0.0	0.0	1.384151055172953E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCCC	80	1.9543477E-6	13.090413	8
CTAGTAC	240	0.0	11.477645	3
TGCACCG	100	2.3451856E-5	10.47233	5
ATCGCCG	185	6.184564E-11	10.252325	19
CGTGCGG	75	0.0026042047	10.154282	10
ACCGCTC	85	6.469786E-4	10.0803175	8
AGGCGTT	90	0.0010928127	9.5203	7
AGACCGT	90	0.0010928127	9.5203	6
CGAATTA	80	0.0045855893	9.482963	15
TAGACAG	475	0.0	9.219659	5
TGTACCG	145	1.6594531E-6	9.192014	5
ACCGGGC	105	4.4006604E-4	9.066953	8
CTGTTCG	105	4.4037183E-4	9.066323	9
CCGAATT	85	0.0075474167	8.92545	14
GTCCTAG	280	0.0	8.829747	1
AATCGCC	215	1.0786607E-9	8.821564	18
GAAATCG	260	7.2759576E-12	8.753706	16
CGCGCCT	380	0.0	8.734611	12
AGGACCG	120	1.7247132E-4	8.726942	5
TACACCG	120	1.7247132E-4	8.726942	5
>>END_MODULE