FastQCFastQC Report
Wed 25 May 2016
SRR1294587_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294587_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1839494
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC57020.3099765478984982No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA47950.26066951020226214No Hit
GTACATGGTAAGCAGTGGTATCAAC42210.2294652768641811No Hit
ACCATGTACTCTGCGTTGATACCAC40730.22141958603833445No Hit
GTGGTAAGCAGTGGTATCAACGCAG37500.20386040943868258No Hit
GTGGTATCAACGCAGAGTACATGGG31120.16917695844618139No Hit
CATGTACTCTGCGTTGATACCACTG29540.16058763986183155No Hit
GGTAAGCAGTGGTATCAACGCAGAG24670.13411296802272799No Hit
GTATCAACGCAGAGTACTTTTTTTT19530.10617050123566589No Hit
GTATCAACGCAGAGTACATGGGAAG18410.10008187034043059No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACCGG400.00528781714.2466566
GTCCTAA1900.012.51510051
TACGACC550.003074213212.0880724
ATACCGT550.003074213212.0880726
AGATCCG600.005887818511.0804315
ACGACCA600.005887818511.0804315
GGGTACG951.342374E-511.01328851
GGTACGA700.001490150110.8584332
GTAGCAC1201.7045566E-59.4980293
AACTGAC1201.7045566E-59.4980297
TAAACTG2451.8189894E-129.3036865
GTTCTAT1856.557457E-99.254411
AGTACTG1856.724804E-99.2408225
GTATAAC1356.742408E-69.15921
CGGGTTC1054.4724956E-49.05164818
GTAGGCC1054.501774E-49.0457423
TAGCTCC950.00182768848.9978884
GTGCAGC1804.1352905E-88.9830611
GACTATG1804.2311513E-88.970367
GCCTTAC850.0073625618.9519791
Produced by FastQC (version 0.11.5)