FastQCFastQC Report
Wed 25 May 2016
SRR1294587_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294587_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1839494
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69260.3765165855392842No Hit
CCCATGTACTCTGCGTTGATACCAC62060.3373753869270571No Hit
GGTATCAACGCAGAGTACTTTTTTT52550.2856763870934072No Hit
GTACATGGTAAGCAGTGGTATCAAC43830.23827204655193224No Hit
ACCATGTACTCTGCGTTGATACCAC43640.2372391538107762No Hit
GTGGTAAGCAGTGGTATCAACGCAG42790.23261831786349943No Hit
TATCAACGCAGAGTACTTTTTTTTT41810.2272907658301685No Hit
GTGGTATCAACGCAGAGTACATGGG31780.1727649016523022No Hit
CATGTACTCTGCGTTGATACCACTG31230.16977494898053486No Hit
GGTAAGCAGTGGTATCAACGCAGAG27640.15025871245027164No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23960.1302532109373556No Hit
ACGCAGAGTACTTTTTTTTTTTTTT22460.1220987945598083No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA21820.1186195769053881No Hit
CCATGTACTCTGCGTTGATACCACT19680.10698594287342061No Hit
GTATCAACGCAGAGTACATGGGAAG19120.10394162742580297No Hit
GGTATCAACGCAGAGTACATGGGAA18920.10285437190879665No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCCGA456.7703566E-414.77570611
CACACCG550.003063917412.0934875
GTCGGCC600.00588249211.08177912
TAACCCG951.3572475E-511.0023465
GCTCTAG856.548415E-410.0664441
GTTACAC1054.0957126E-59.9545043
TAAGACT1701.6585545E-89.5020274
TGCACTG3900.09.2583855
GCTACAC1356.827977E-69.1500993
GTCTAGA1252.7293756E-59.1269081
CACCGCT1054.4901983E-49.0480727
CCGAATA1054.4950825E-49.0470899
TCCTATA2002.6411726E-99.0274152
GTATAGG1901.0437361E-89.0068191
ACTGGTC1701.6852027E-78.9408958
CTACACT2451.6370905E-118.9202694
AAGGTGT1606.718201E-78.908156
GCTATAC1401.08200275E-58.823313
TAGACAG2607.2759576E-128.77115
GTCTTGC3150.08.7526571
Produced by FastQC (version 0.11.5)