FastQCFastQC Report
Wed 25 May 2016
SRR1294586_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294586_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144205
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC43050.3762437675066968No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA40000.34958770500041514No Hit
GTACATGGTAAGCAGTGGTATCAAC34930.3052774633916125No Hit
ACCATGTACTCTGCGTTGATACCAC32980.2882350627728423No Hit
GTGGTAAGCAGTGGTATCAACGCAG24980.21831752177275923No Hit
CATGTACTCTGCGTTGATACCACTG20620.180212461927714No Hit
GTGGTATCAACGCAGAGTACATGGG19220.1679768922526995No Hit
GGTAAGCAGTGGTATCAACGCAGAG19040.1664037475801976No Hit
ATTCCATTCCATTCCATTCCATTCC15630.13660139572891222No Hit
CCATGTACTCTGCGTTGATACCACT14960.13074580167015526No Hit
GTATCAACGCAGAGTACATGGGAAG13750.1201707735938927No Hit
GCTTACCATGTACTCTGCGTTGATA13300.11623791191263802No Hit
GAGTACATGGTAAGCAGTGGTATCA13200.115363942650137No Hit
GAATGGAATGGAATGGAATGGAATG12750.11143108096888232No Hit
GGTATCAACGCAGAGTACATGGGAA12160.1062746623201262No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA12130.10601247154137589No Hit
ACTCTGCGTTGATACCACTGCTTAC12020.10505110535262475No Hit
GTATCAACGCAGAGTACTTTTTTTT11600.10138043445012039No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG802.8223165E-511.892351
GGCTTAC657.9229276E-411.7093921
TATTAGG1053.465957E-610.8563682
CTAGGAG909.53711E-510.5524953
GTCTTAG1002.3585348E-510.4652681
CTTACAC1409.713767E-810.1756213
GTATTAG1701.5170372E-910.073521
CTAGCAC951.6477752E-49.9971013
GTATTGG1054.0363433E-59.9669231
GTTTTAC1054.0363433E-59.9669231
TAGACTG1451.6201739E-79.8251675
GTCTTAC1106.722175E-59.5138811
GTTATAT2002.564775E-109.5138811
GTACTAG800.00445986269.513881
CTAGACA1002.760224E-49.4976624
TAGGACA900.00111554959.4976614
CCTATAC1106.852129E-59.4972473
CTAGGAC900.00111599569.4972463
GTGCTAA1651.0398253E-79.2255811
TGTAGGA1451.7052662E-69.1717592
Produced by FastQC (version 0.11.5)