FastQCFastQC Report
Wed 25 May 2016
SRR1294586_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294586_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144205
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44240.38664400173045915No Hit
CCCATGTACTCTGCGTTGATACCAC43450.37973964455670095No Hit
GTACATGGTAAGCAGTGGTATCAAC38820.3392748677029029No Hit
ACCATGTACTCTGCGTTGATACCAC35170.307374989621615No Hit
GGTATCAACGCAGAGTACTTTTTTT30360.2653370680953151No Hit
GTGGTAAGCAGTGGTATCAACGCAG30000.2621907787503114No Hit
TATCAACGCAGAGTACTTTTTTTTT25980.22705721439776963No Hit
GGTAAGCAGTGGTATCAACGCAGAG22570.19725486254648425No Hit
CATGTACTCTGCGTTGATACCACTG22350.19533213016898193No Hit
GTGGTATCAACGCAGAGTACATGGG19340.16902565536770073No Hit
ATTCCATTCCATTCCATTCCATTCC16770.14656464532142405No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA16710.1460402637639234No Hit
CCATGTACTCTGCGTTGATACCACT15730.13747536499141325No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15690.13712577728641284No Hit
GTATCAACGCAGAGTACATGGGAAG15140.13231894634265712No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14880.13004662626015442No Hit
GAGTACATGGTAAGCAGTGGTATCA14500.1267255430626505No Hit
GGTATCAACGCAGAGTACATGGGAA13880.12130693363514407No Hit
ACTCTGCGTTGATACCACTGCTTAC13570.11859762892139084No Hit
GTACATGGGAAGCAGTGGTAAGCAG13140.11483956109263638No Hit
GAATGGAATGGAATGGAATGGAATG13090.11440257646138585No Hit
GCTTACCATGTACTCTGCGTTGATA12980.11344121027263472No Hit
GCAGTGGTATCAACGCAGAGTACAT12620.11029492092763098No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11820.10330316682762267No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG250.006027922519.0017495
CTGTCGC550.003068493212.0904369
TCAGTAC658.0143934E-411.6933833
GTCTAAC752.0610628E-411.4070321
CCTACAC855.3147985E-511.1774993
TCTATAC1553.110472E-1011.0332733
ACAGATC1303.263085E-810.9611098
CTAAGAC909.5002586E-510.5565263
GTATTAG1855.820766E-1110.2766061
GTACTGC750.002633151410.1395851
TAGACTG1606.150003E-910.0946795
GTCCTAG856.552853E-410.0650281
CCAGTAC951.6414368E-410.000923
TATACAG2500.09.880915
TAACACT1356.425307E-79.8527584
CTATACT1651.0182703E-89.7887794
AGGACAG1856.4574124E-109.7576555
GTTCTAT1106.7845045E-59.505861
GTATTAC1304.20003E-69.505861
GTATAGC1304.20003E-69.505861
Produced by FastQC (version 0.11.5)