FastQCFastQC Report
Wed 25 May 2016
SRR1294585_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294585_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794919
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC173900.9688459479229982No Hit
ACCATGTACTCTGCGTTGATACCAC137800.7677226660367403No Hit
GTACATGGTAAGCAGTGGTATCAAC135380.7542401634837004No Hit
GTGGTAAGCAGTGGTATCAACGCAG106490.593285825154227No Hit
CATGTACTCTGCGTTGATACCACTG88830.4948969842093153No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA87380.4868186252415847No Hit
GGTAAGCAGTGGTATCAACGCAGAG80340.4475967996327411No Hit
CCATGTACTCTGCGTTGATACCACT62350.34736943561241485No Hit
ACTCTGCGTTGATACCACTGCTTAC58190.32419290229809816No Hit
GAGTACATGGTAAGCAGTGGTATCA50730.282631138229636No Hit
GTATCAACGCAGAGTACATGGGAAG49630.27650272797825415No Hit
GCAGTGGTATCAACGCAGAGTACAT47320.26363306645035234No Hit
GTACTCTGCGTTGATACCACTGCTT45640.2542733126118783No Hit
GTACATGGGAAGCAGTGGTAAGCAG45120.25137624594758873No Hit
GCTTACCATGTACTCTGCGTTGATA44970.25054055364058214No Hit
GTGGTATCAACGCAGAGTACATGGG44340.24703064595115432No Hit
GCGTTGATACCACTGCTTACCATGT42370.2360552203191342No Hit
GGTATCAACGCAGAGTACATGGGAA40020.22296270750936392No Hit
CTGCTTACCATGTACTCTGCGTTGA37350.20808738444464625No Hit
GCTTACCACTGCTTCCCATGTACTC36500.2033517947049421No Hit
GCGTTGATACCACTGCTTACCACTG36330.2024046767570013No Hit
GTATCAACGCAGAGTACATGGTAAG33870.18869932292209288No Hit
GTACATGGGAAGCAGTGGTATCAAC32920.18340660497771766No Hit
AAGCAGTGGTATCAACGCAGAGTAC32300.179952410108757No Hit
GTACATGATAAGCAGTGGTATCAAC31080.17315544601176988No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA30440.169589825501875No Hit
TATCAACGCAGAGTACATGGGAAGC29250.16295999986628923No Hit
CAGTGGTATCAACGCAGAGTACATG28420.1583358357675193No Hit
ATCATGTACTCTGCGTTGATACCAC28180.15699872807630874No Hit
GTACATAAGCAGTGGTATCAACGCA27540.15343310756641387No Hit
ATGGTAAGCAGTGGTATCAACGCAG27110.15103745628632823No Hit
TATCAACGCAGAGTACATGGTAAGC26040.14507618449634774No Hit
ACGCAGAGTACATGGTAAGCAGTGG26010.14490904603494642No Hit
GTATCAACGCAGAGTACTTTTTTTT23330.12997801014976162No Hit
GAGTAAGCAGTGGTATCAACGCAGA22110.12318104605277454No Hit
GGTATCAACGCAGAGTACATGGTAA20280.11298559990729387No Hit
TACCACTGCTTACCATGTACTCTGC20270.11292988708682676No Hit
ACGCAGAGTACATGGGAAGCAGTGG20200.11253989734355702No Hit
GTGGTATCAACGCAGAGTACATGGT19590.10914141529506345No Hit
GAGTACATGGGAAGCAGTGGTAAGC19530.1088071383722608No Hit
GTGATAAGCAGTGGTATCAACGCAG19360.10786002042432No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGGT453.5440127E-516.8863124
GCGATAA350.002154856216.3032191
CGGTCTC456.773738E-414.77477
AGCCTCG1650.014.3949532
GTCTTAA1203.6379788E-1214.2653171
TCGTGGG1501.8189894E-1212.66402912
CTCGTGG1501.8189894E-1212.66367611
CGTGGGG1405.2750693E-1112.21242113
AGAACGG550.003072912312.0887295
GAACAAA5550.011.4807961
TATTCCG752.0776333E-411.39794355
CTTAGAC700.001493149610.8557893
TACAAGC1158.809511E-710.7398872
GCCGCGG1158.8316665E-710.7377933
GCTCTAC803.7340296E-410.6989881
GTGCTAG1201.50286E-610.3027291
CATAAGC6200.010.1113614
GTACAGA1909.822543E-1110.0107491
GTCTAGT1054.056993E-59.96307851
AGCCGCG1054.101996E-59.9530812
Produced by FastQC (version 0.11.5)