FastQCFastQC Report
Wed 25 May 2016
SRR1294585_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1294585_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794919
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC184781.0294614965912112No Hit
ACCATGTACTCTGCGTTGATACCAC149160.8310124300873745No Hit
GTACATGGTAAGCAGTGGTATCAAC142820.7956905019112284No Hit
GTGGTAAGCAGTGGTATCAACGCAG117960.6571884302299993No Hit
CATGTACTCTGCGTTGATACCACTG91680.5107751380424409No Hit
GGTAAGCAGTGGTATCAACGCAGAG91000.5069866662506776No Hit
GTATCAACGCAGAGTACTTTTTTTT83280.4639763688500707No Hit
CCATGTACTCTGCGTTGATACCACT66310.3694317125173894No Hit
ACTCTGCGTTGATACCACTGCTTAC60860.33906822536281583No Hit
GAGTACATGGTAAGCAGTGGTATCA55020.30653193821002506No Hit
GTATCAACGCAGAGTACATGGGAAG52760.2939408407844588No Hit
TATCAACGCAGAGTACTTTTTTTTT52030.28987380489035997No Hit
GGTATCAACGCAGAGTACTTTTTTT51860.28892668694241913No Hit
GCAGTGGTATCAACGCAGAGTACAT51690.2879795689944783No Hit
GTACATGGGAAGCAGTGGTAAGCAG48420.26976147670173417No Hit
GCTTACCATGTACTCTGCGTTGATA47230.2631316510661484No Hit
GTGGTATCAACGCAGAGTACATGGG46180.2572818049171021No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA45420.25304763056160196No Hit
GTACTCTGCGTTGATACCACTGCTT44240.24647351774648327No Hit
GCGTTGATACCACTGCTTACCATGT43040.2397879792904304No Hit
GGTATCAACGCAGAGTACATGGGAA41870.2332695792957788No Hit
AAGCAGTGGTATCAACGCAGAGTAC38200.2128229741843504No Hit
GTACTTTTTTTTTTTTTTTTTTTTT37940.21137444085220558No Hit
CTGCTTACCATGTACTCTGCGTTGA36840.20524603060082378No Hit
GCTTACCACTGCTTCCCATGTACTC36580.20379749726867896No Hit
GCGTTGATACCACTGCTTACCACTG36380.20268324085933684No Hit
GTACATGGGAAGCAGTGGTATCAAC35010.1950505844553431No Hit
GTATCAACGCAGAGTACATGGTAAG34860.1942148921483365No Hit
GTACATGATAAGCAGTGGTATCAAC32320.1800638357496912No Hit
ATGGTAAGCAGTGGTATCAACGCAG32050.1785595895970793No Hit
TATCAACGCAGAGTACATGGGAAGC31070.17309973319130278No Hit
CAGTGGTATCAACGCAGAGTACATG30940.1723754665252304No Hit
ATCATGTACTCTGCGTTGATACCAC30580.17036980498841453No Hit
GTACATAAGCAGTGGTATCAACGCA28300.15766728192191404No Hit
ACGCAGAGTACATGGTAAGCAGTGG27810.15493735371902578No Hit
TATCAACGCAGAGTACATGGTAAGC27630.15393452295061782No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27250.15181743577286774No Hit
GAGTACTTTTTTTTTTTTTTTTTTT25970.144686194753078No Hit
GAGTAAGCAGTGGTATCAACGCAGA25510.14212340501159104No Hit
GTGATAAGCAGTGGTATCAACGCAG22920.12769378451061023No Hit
ACGCAGAGTACATGGGAAGCAGTGG22300.12423958964164956No Hit
GGTATCAACGCAGAGTACATGGTAA22030.12273534348903765No Hit
GTGGTATCAACGCAGAGTACATGGT20800.11588266657158346No Hit
GAGTACATGGGAAGCAGTGGTAAGC20760.11565981528971503No Hit
TACCACTGCTTACCATGTACTCTGC20010.11148135375468196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACA456.75456E-414.7802274
CGCTAAG551.9609054E-413.8177777
CACCGTT500.001500036713.2996097
GCACCGT604.0868574E-412.6687656
CACCGAA658.02646E-411.6919647
ACGCTAA700.001489537110.8589426
TTAGCAC2700.010.5573043
TCTAGAC1106.0337934E-610.3653553
AACGCTA750.002644274610.1350145
TAGGAGT1606.153641E-910.0954234
ATAATAC3050.09.9688653
TAGCACT3350.09.643394
TCAGGAC1002.7507887E-49.50157453
CCGGGCA900.0011145579.4991919
AGCACTG4450.09.39481455
GTCCTAG1651.0571239E-79.216731
GTACTAA1651.0571239E-79.216731
AAGGTGC1651.06256266E-79.2136486
CTACACC1651.06256266E-79.2136484
GTGCTAA2900.09.1770031
Produced by FastQC (version 0.11.5)